Streptococcus phage phi-SsUD.1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E4ZFJ4|E4ZFJ4_9CAUD Peptidase_S24 domain-containing protein OS=Streptococcus phage phi-SsUD.1 OX=861448 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 10.52LKK4 pKa = 10.66DD5 pKa = 3.35ILEE8 pKa = 4.55LGTYY12 pKa = 9.43GFNPNCKK19 pKa = 9.81VEE21 pKa = 4.25IFCMDD26 pKa = 3.23NFEE29 pKa = 4.53EE30 pKa = 4.31RR31 pKa = 11.84LEE33 pKa = 4.14NEE35 pKa = 4.24GFDD38 pKa = 5.31EE39 pKa = 5.48ILIPQNDD46 pKa = 3.8DD47 pKa = 3.08AKK49 pKa = 10.01IYY51 pKa = 9.91PYY53 pKa = 11.18AFLVEE58 pKa = 4.3DD59 pKa = 4.59SILIAMTEE67 pKa = 3.91EE68 pKa = 4.47DD69 pKa = 5.42DD70 pKa = 4.4NTNEE74 pKa = 4.2CC75 pKa = 4.51

Molecular weight: 8.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E4ZFP2|E4ZFP2_9CAUD RibD_C domain-containing protein OS=Streptococcus phage phi-SsUD.1 OX=861448 PE=4 SV=1
MM1 pKa = 7.22GQPPQNYY8 pKa = 8.4QRR10 pKa = 11.84LSSARR15 pKa = 11.84YY16 pKa = 8.57KK17 pKa = 10.9DD18 pKa = 3.47SSLTFYY24 pKa = 10.15ATTPHH29 pKa = 6.44TPHH32 pKa = 7.09ALSGQKK38 pKa = 9.56RR39 pKa = 11.84CRR41 pKa = 11.84TPLVSPWRR49 pKa = 11.84KK50 pKa = 9.41PIFLKK55 pKa = 10.61QSPGG59 pKa = 3.15

Molecular weight: 6.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

18008

53

1400

272.8

30.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.364 ± 0.337

1.038 ± 0.12

5.781 ± 0.246

7.38 ± 0.282

3.976 ± 0.18

6.236 ± 0.236

1.888 ± 0.135

7.008 ± 0.245

7.502 ± 0.232

9.179 ± 0.242

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.366 ± 0.14

4.903 ± 0.165

3.088 ± 0.187

4.265 ± 0.212

4.565 ± 0.193

6.508 ± 0.323

6.253 ± 0.404

6.331 ± 0.205

1.294 ± 0.087

4.07 ± 0.259
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski