Amino acid dipepetide frequency for Streptococcus phage phi-SsUD.1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.221AlaAla: 4.221 ± 0.932
0.389AlaCys: 0.389 ± 0.135
4.11AlaAsp: 4.11 ± 0.505
4.665AlaGlu: 4.665 ± 0.447
2.999AlaPhe: 2.999 ± 0.338
4.11AlaGly: 4.11 ± 0.516
0.555AlaHis: 0.555 ± 0.166
5.609AlaIle: 5.609 ± 0.848
5.109AlaLys: 5.109 ± 0.449
5.664AlaLeu: 5.664 ± 0.646
1.666AlaMet: 1.666 ± 0.269
2.999AlaAsn: 2.999 ± 0.43
1.555AlaPro: 1.555 ± 0.354
2.443AlaGln: 2.443 ± 0.515
2.443AlaArg: 2.443 ± 0.337
3.832AlaSer: 3.832 ± 0.57
3.832AlaThr: 3.832 ± 0.638
3.998AlaVal: 3.998 ± 0.508
0.611AlaTrp: 0.611 ± 0.201
2.832AlaTyr: 2.832 ± 0.403
0.0AlaXaa: 0.0 ± 0.0
Cys
0.611CysAla: 0.611 ± 0.196
0.278CysCys: 0.278 ± 0.123
0.389CysAsp: 0.389 ± 0.134
0.555CysGlu: 0.555 ± 0.169
0.444CysPhe: 0.444 ± 0.143
0.777CysGly: 0.777 ± 0.191
0.222CysHis: 0.222 ± 0.11
0.555CysIle: 0.555 ± 0.182
0.722CysLys: 0.722 ± 0.265
0.833CysLeu: 0.833 ± 0.23
0.222CysMet: 0.222 ± 0.15
0.389CysAsn: 0.389 ± 0.149
0.333CysPro: 0.333 ± 0.11
0.722CysGln: 0.722 ± 0.218
0.777CysArg: 0.777 ± 0.227
0.666CysSer: 0.666 ± 0.227
0.333CysThr: 0.333 ± 0.112
0.944CysVal: 0.944 ± 0.236
0.0CysTrp: 0.0 ± 0.0
0.611CysTyr: 0.611 ± 0.21
0.0CysXaa: 0.0 ± 0.0
Asp
2.999AspAla: 2.999 ± 0.337
0.777AspCys: 0.777 ± 0.248
3.11AspAsp: 3.11 ± 0.56
5.387AspGlu: 5.387 ± 0.578
3.221AspPhe: 3.221 ± 0.365
4.554AspGly: 4.554 ± 0.5
0.833AspHis: 0.833 ± 0.216
4.943AspIle: 4.943 ± 0.471
3.943AspLys: 3.943 ± 0.389
5.331AspLeu: 5.331 ± 0.793
1.944AspMet: 1.944 ± 0.304
3.221AspAsn: 3.221 ± 0.469
1.555AspPro: 1.555 ± 0.332
1.277AspGln: 1.277 ± 0.266
1.999AspArg: 1.999 ± 0.424
3.554AspSer: 3.554 ± 0.498
2.832AspThr: 2.832 ± 0.357
2.61AspVal: 2.61 ± 0.37
0.777AspTrp: 0.777 ± 0.192
2.943AspTyr: 2.943 ± 0.465
0.0AspXaa: 0.0 ± 0.0
Glu
3.943GluAla: 3.943 ± 0.447
0.777GluCys: 0.777 ± 0.209
4.221GluAsp: 4.221 ± 0.536
5.998GluGlu: 5.998 ± 0.745
2.277GluPhe: 2.277 ± 0.397
3.943GluGly: 3.943 ± 0.448
1.333GluHis: 1.333 ± 0.29
4.776GluIle: 4.776 ± 0.559
7.219GluLys: 7.219 ± 0.701
8.33GluLeu: 8.33 ± 0.701
2.277GluMet: 2.277 ± 0.475
4.498GluAsn: 4.498 ± 0.525
1.777GluPro: 1.777 ± 0.389
3.887GluGln: 3.887 ± 0.404
3.165GluArg: 3.165 ± 0.39
3.61GluSer: 3.61 ± 0.326
4.498GluThr: 4.498 ± 0.542
4.221GluVal: 4.221 ± 0.394
0.944GluTrp: 0.944 ± 0.248
2.055GluTyr: 2.055 ± 0.38
0.0GluXaa: 0.0 ± 0.0
Phe
2.499PheAla: 2.499 ± 0.339
0.555PheCys: 0.555 ± 0.152
3.165PheAsp: 3.165 ± 0.542
3.165PheGlu: 3.165 ± 0.405
1.722PhePhe: 1.722 ± 0.296
2.721PheGly: 2.721 ± 0.336
1.166PheHis: 1.166 ± 0.227
2.666PheIle: 2.666 ± 0.324
2.832PheLys: 2.832 ± 0.499
3.499PheLeu: 3.499 ± 0.551
0.833PheMet: 0.833 ± 0.227
2.388PheAsn: 2.388 ± 0.311
0.944PhePro: 0.944 ± 0.25
1.499PheGln: 1.499 ± 0.368
1.555PheArg: 1.555 ± 0.258
1.722PheSer: 1.722 ± 0.276
1.999PheThr: 1.999 ± 0.252
2.221PheVal: 2.221 ± 0.328
0.777PheTrp: 0.777 ± 0.204
1.833PheTyr: 1.833 ± 0.288
0.0PheXaa: 0.0 ± 0.0
Gly
3.165GlyAla: 3.165 ± 0.499
0.5GlyCys: 0.5 ± 0.164
3.277GlyAsp: 3.277 ± 0.52
4.054GlyGlu: 4.054 ± 0.458
2.888GlyPhe: 2.888 ± 0.387
3.776GlyGly: 3.776 ± 0.646
1.833GlyHis: 1.833 ± 0.355
5.664GlyIle: 5.664 ± 0.589
4.443GlyLys: 4.443 ± 0.374
5.442GlyLeu: 5.442 ± 0.697
1.722GlyMet: 1.722 ± 0.342
3.499GlyAsn: 3.499 ± 0.462
0.666GlyPro: 0.666 ± 0.16
3.11GlyGln: 3.11 ± 0.442
3.054GlyArg: 3.054 ± 0.404
3.665GlySer: 3.665 ± 0.434
4.054GlyThr: 4.054 ± 0.443
4.11GlyVal: 4.11 ± 0.552
0.611GlyTrp: 0.611 ± 0.156
2.832GlyTyr: 2.832 ± 0.327
0.0GlyXaa: 0.0 ± 0.0
His
1.0HisAla: 1.0 ± 0.243
0.222HisCys: 0.222 ± 0.111
1.0HisAsp: 1.0 ± 0.25
1.055HisGlu: 1.055 ± 0.242
0.944HisPhe: 0.944 ± 0.223
1.388HisGly: 1.388 ± 0.237
0.555HisHis: 0.555 ± 0.175
1.388HisIle: 1.388 ± 0.271
1.055HisLys: 1.055 ± 0.22
2.055HisLeu: 2.055 ± 0.321
0.444HisMet: 0.444 ± 0.182
0.722HisAsn: 0.722 ± 0.194
0.944HisPro: 0.944 ± 0.234
0.944HisGln: 0.944 ± 0.232
0.889HisArg: 0.889 ± 0.201
0.666HisSer: 0.666 ± 0.161
1.333HisThr: 1.333 ± 0.277
1.333HisVal: 1.333 ± 0.283
0.222HisTrp: 0.222 ± 0.104
0.722HisTyr: 0.722 ± 0.182
0.0HisXaa: 0.0 ± 0.0
Ile
4.887IleAla: 4.887 ± 0.463
0.389IleCys: 0.389 ± 0.133
5.276IleAsp: 5.276 ± 0.596
4.887IleGlu: 4.887 ± 0.588
2.11IlePhe: 2.11 ± 0.385
5.054IleGly: 5.054 ± 0.578
1.388IleHis: 1.388 ± 0.285
3.776IleIle: 3.776 ± 0.405
5.387IleLys: 5.387 ± 0.548
5.942IleLeu: 5.942 ± 0.531
1.111IleMet: 1.111 ± 0.219
3.054IleAsn: 3.054 ± 0.35
2.388IlePro: 2.388 ± 0.234
2.888IleGln: 2.888 ± 0.324
3.11IleArg: 3.11 ± 0.383
5.553IleSer: 5.553 ± 0.73
4.609IleThr: 4.609 ± 0.779
4.776IleVal: 4.776 ± 0.53
0.944IleTrp: 0.944 ± 0.29
2.555IleTyr: 2.555 ± 0.372
0.0IleXaa: 0.0 ± 0.0
Lys
5.331LysAla: 5.331 ± 0.435
0.5LysCys: 0.5 ± 0.157
3.887LysAsp: 3.887 ± 0.578
5.498LysGlu: 5.498 ± 0.525
2.721LysPhe: 2.721 ± 0.378
4.831LysGly: 4.831 ± 0.442
1.666LysHis: 1.666 ± 0.304
5.72LysIle: 5.72 ± 0.488
5.498LysLys: 5.498 ± 0.761
7.275LysLeu: 7.275 ± 0.575
1.999LysMet: 1.999 ± 0.297
3.499LysAsn: 3.499 ± 0.423
2.443LysPro: 2.443 ± 0.317
3.554LysGln: 3.554 ± 0.528
3.554LysArg: 3.554 ± 0.474
4.11LysSer: 4.11 ± 0.561
3.887LysThr: 3.887 ± 0.445
5.276LysVal: 5.276 ± 0.475
0.944LysTrp: 0.944 ± 0.276
2.832LysTyr: 2.832 ± 0.46
0.0LysXaa: 0.0 ± 0.0
Leu
6.442LeuAla: 6.442 ± 0.7
0.944LeuCys: 0.944 ± 0.244
5.165LeuAsp: 5.165 ± 0.534
7.775LeuGlu: 7.775 ± 0.696
3.887LeuPhe: 3.887 ± 0.522
4.887LeuGly: 4.887 ± 0.486
1.722LeuHis: 1.722 ± 0.221
4.665LeuIle: 4.665 ± 0.421
7.33LeuLys: 7.33 ± 0.485
6.997LeuLeu: 6.997 ± 0.608
1.944LeuMet: 1.944 ± 0.258
4.443LeuAsn: 4.443 ± 0.624
3.388LeuPro: 3.388 ± 0.427
3.221LeuGln: 3.221 ± 0.475
3.998LeuArg: 3.998 ± 0.434
7.608LeuSer: 7.608 ± 0.593
6.442LeuThr: 6.442 ± 0.639
6.053LeuVal: 6.053 ± 0.518
0.666LeuTrp: 0.666 ± 0.152
4.165LeuTyr: 4.165 ± 0.588
0.056LeuXaa: 0.056 ± 0.05
Met
1.444MetAla: 1.444 ± 0.296
0.111MetCys: 0.111 ± 0.08
1.833MetAsp: 1.833 ± 0.275
1.555MetGlu: 1.555 ± 0.322
0.889MetPhe: 0.889 ± 0.207
1.722MetGly: 1.722 ± 0.259
0.222MetHis: 0.222 ± 0.099
1.999MetIle: 1.999 ± 0.347
1.666MetLys: 1.666 ± 0.302
1.833MetLeu: 1.833 ± 0.344
0.889MetMet: 0.889 ± 0.212
1.166MetAsn: 1.166 ± 0.262
0.611MetPro: 0.611 ± 0.183
0.777MetGln: 0.777 ± 0.182
1.277MetArg: 1.277 ± 0.232
2.221MetSer: 2.221 ± 0.374
1.444MetThr: 1.444 ± 0.309
1.111MetVal: 1.111 ± 0.244
0.222MetTrp: 0.222 ± 0.089
0.666MetTyr: 0.666 ± 0.167
0.0MetXaa: 0.0 ± 0.0
Asn
3.499AsnAla: 3.499 ± 0.599
0.5AsnCys: 0.5 ± 0.148
2.443AsnAsp: 2.443 ± 0.323
2.777AsnGlu: 2.777 ± 0.384
2.055AsnPhe: 2.055 ± 0.374
4.054AsnGly: 4.054 ± 0.561
1.222AsnHis: 1.222 ± 0.228
3.054AsnIle: 3.054 ± 0.371
3.277AsnLys: 3.277 ± 0.424
4.831AsnLeu: 4.831 ± 0.684
1.055AsnMet: 1.055 ± 0.224
2.166AsnAsn: 2.166 ± 0.368
2.055AsnPro: 2.055 ± 0.249
2.332AsnGln: 2.332 ± 0.34
2.499AsnArg: 2.499 ± 0.349
2.999AsnSer: 2.999 ± 0.318
2.332AsnThr: 2.332 ± 0.415
2.832AsnVal: 2.832 ± 0.384
1.166AsnTrp: 1.166 ± 0.27
1.888AsnTyr: 1.888 ± 0.378
0.0AsnXaa: 0.0 ± 0.0
Pro
1.499ProAla: 1.499 ± 0.242
0.5ProCys: 0.5 ± 0.15
1.499ProAsp: 1.499 ± 0.275
2.332ProGlu: 2.332 ± 0.372
1.222ProPhe: 1.222 ± 0.226
1.055ProGly: 1.055 ± 0.309
0.555ProHis: 0.555 ± 0.18
2.277ProIle: 2.277 ± 0.419
2.666ProLys: 2.666 ± 0.359
2.999ProLeu: 2.999 ± 0.403
0.5ProMet: 0.5 ± 0.147
1.61ProAsn: 1.61 ± 0.301
1.055ProPro: 1.055 ± 0.222
0.944ProGln: 0.944 ± 0.225
1.722ProArg: 1.722 ± 0.284
2.332ProSer: 2.332 ± 0.327
2.11ProThr: 2.11 ± 0.362
1.999ProVal: 1.999 ± 0.346
0.333ProTrp: 0.333 ± 0.116
1.666ProTyr: 1.666 ± 0.293
0.0ProXaa: 0.0 ± 0.0
Gln
3.887GlnAla: 3.887 ± 0.54
0.5GlnCys: 0.5 ± 0.161
2.277GlnAsp: 2.277 ± 0.325
2.777GlnGlu: 2.777 ± 0.349
1.499GlnPhe: 1.499 ± 0.301
2.166GlnGly: 2.166 ± 0.32
0.611GlnHis: 0.611 ± 0.142
2.555GlnIle: 2.555 ± 0.382
2.555GlnLys: 2.555 ± 0.483
4.276GlnLeu: 4.276 ± 0.445
1.055GlnMet: 1.055 ± 0.236
2.332GlnAsn: 2.332 ± 0.371
1.499GlnPro: 1.499 ± 0.307
1.388GlnGln: 1.388 ± 0.312
1.999GlnArg: 1.999 ± 0.298
2.499GlnSer: 2.499 ± 0.423
3.054GlnThr: 3.054 ± 0.618
3.832GlnVal: 3.832 ± 0.558
0.833GlnTrp: 0.833 ± 0.244
1.055GlnTyr: 1.055 ± 0.244
0.0GlnXaa: 0.0 ± 0.0
Arg
2.332ArgAla: 2.332 ± 0.36
0.666ArgCys: 0.666 ± 0.23
2.555ArgAsp: 2.555 ± 0.437
3.554ArgGlu: 3.554 ± 0.428
1.444ArgPhe: 1.444 ± 0.318
2.166ArgGly: 2.166 ± 0.285
0.666ArgHis: 0.666 ± 0.202
3.443ArgIle: 3.443 ± 0.468
4.276ArgLys: 4.276 ± 0.628
4.609ArgLeu: 4.609 ± 0.493
0.833ArgMet: 0.833 ± 0.229
2.277ArgAsn: 2.277 ± 0.32
1.388ArgPro: 1.388 ± 0.236
2.332ArgGln: 2.332 ± 0.311
1.999ArgArg: 1.999 ± 0.342
2.721ArgSer: 2.721 ± 0.328
2.721ArgThr: 2.721 ± 0.381
2.832ArgVal: 2.832 ± 0.415
0.777ArgTrp: 0.777 ± 0.212
2.055ArgTyr: 2.055 ± 0.37
0.0ArgXaa: 0.0 ± 0.0
Ser
4.443SerAla: 4.443 ± 0.55
0.611SerCys: 0.611 ± 0.162
3.332SerAsp: 3.332 ± 0.453
4.387SerGlu: 4.387 ± 0.379
2.61SerPhe: 2.61 ± 0.459
4.11SerGly: 4.11 ± 0.475
1.555SerHis: 1.555 ± 0.281
5.387SerIle: 5.387 ± 0.618
4.165SerLys: 4.165 ± 0.463
5.387SerLeu: 5.387 ± 0.519
1.444SerMet: 1.444 ± 0.254
3.054SerAsn: 3.054 ± 0.499
2.166SerPro: 2.166 ± 0.344
2.943SerGln: 2.943 ± 0.58
3.388SerArg: 3.388 ± 0.489
4.554SerSer: 4.554 ± 0.806
3.943SerThr: 3.943 ± 0.424
3.665SerVal: 3.665 ± 0.461
1.111SerTrp: 1.111 ± 0.16
2.832SerTyr: 2.832 ± 0.461
0.0SerXaa: 0.0 ± 0.0
Thr
4.387ThrAla: 4.387 ± 0.573
0.333ThrCys: 0.333 ± 0.123
3.332ThrAsp: 3.332 ± 0.482
4.387ThrGlu: 4.387 ± 0.457
1.999ThrPhe: 1.999 ± 0.426
4.387ThrGly: 4.387 ± 0.637
0.777ThrHis: 0.777 ± 0.19
4.11ThrIle: 4.11 ± 0.641
4.609ThrLys: 4.609 ± 0.463
5.664ThrLeu: 5.664 ± 0.509
1.277ThrMet: 1.277 ± 0.252
2.332ThrAsn: 2.332 ± 0.276
2.499ThrPro: 2.499 ± 0.466
2.277ThrGln: 2.277 ± 0.497
2.388ThrArg: 2.388 ± 0.341
4.72ThrSer: 4.72 ± 0.693
4.665ThrThr: 4.665 ± 0.778
5.109ThrVal: 5.109 ± 0.618
0.666ThrTrp: 0.666 ± 0.211
2.61ThrTyr: 2.61 ± 0.468
0.0ThrXaa: 0.0 ± 0.0
Val
3.554ValAla: 3.554 ± 0.555
0.611ValCys: 0.611 ± 0.194
3.665ValAsp: 3.665 ± 0.516
4.887ValGlu: 4.887 ± 0.589
2.555ValPhe: 2.555 ± 0.469
3.832ValGly: 3.832 ± 0.558
0.944ValHis: 0.944 ± 0.181
4.332ValIle: 4.332 ± 0.479
4.609ValLys: 4.609 ± 0.502
5.776ValLeu: 5.776 ± 0.497
1.111ValMet: 1.111 ± 0.232
2.555ValAsn: 2.555 ± 0.351
2.11ValPro: 2.11 ± 0.31
2.777ValGln: 2.777 ± 0.412
3.11ValArg: 3.11 ± 0.433
4.665ValSer: 4.665 ± 0.61
4.776ValThr: 4.776 ± 0.466
4.11ValVal: 4.11 ± 0.388
0.889ValTrp: 0.889 ± 0.225
2.443ValTyr: 2.443 ± 0.328
0.0ValXaa: 0.0 ± 0.0
Trp
0.889TrpAla: 0.889 ± 0.239
0.222TrpCys: 0.222 ± 0.102
0.389TrpAsp: 0.389 ± 0.147
1.222TrpGlu: 1.222 ± 0.263
0.722TrpPhe: 0.722 ± 0.2
0.611TrpGly: 0.611 ± 0.147
0.278TrpHis: 0.278 ± 0.121
0.722TrpIle: 0.722 ± 0.177
0.722TrpLys: 0.722 ± 0.216
0.944TrpLeu: 0.944 ± 0.195
0.444TrpMet: 0.444 ± 0.136
1.277TrpAsn: 1.277 ± 0.281
0.056TrpPro: 0.056 ± 0.052
0.777TrpGln: 0.777 ± 0.221
0.555TrpArg: 0.555 ± 0.215
0.833TrpSer: 0.833 ± 0.278
1.111TrpThr: 1.111 ± 0.261
0.611TrpVal: 0.611 ± 0.174
0.222TrpTrp: 0.222 ± 0.115
0.333TrpTyr: 0.333 ± 0.126
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.61TyrAla: 2.61 ± 0.37
1.0TyrCys: 1.0 ± 0.255
2.888TyrAsp: 2.888 ± 0.484
2.888TyrGlu: 2.888 ± 0.405
1.555TyrPhe: 1.555 ± 0.344
2.221TyrGly: 2.221 ± 0.325
0.777TyrHis: 0.777 ± 0.18
2.443TyrIle: 2.443 ± 0.417
2.943TyrLys: 2.943 ± 0.461
4.11TyrLeu: 4.11 ± 0.479
0.833TyrMet: 0.833 ± 0.226
1.555TyrAsn: 1.555 ± 0.335
1.444TyrPro: 1.444 ± 0.251
2.499TyrGln: 2.499 ± 0.352
2.166TyrArg: 2.166 ± 0.267
2.555TyrSer: 2.555 ± 0.357
2.555TyrThr: 2.555 ± 0.445
1.666TyrVal: 1.666 ± 0.259
0.222TyrTrp: 0.222 ± 0.098
1.777TyrTyr: 1.777 ± 0.344
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.056XaaGln: 0.056 ± 0.05
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 66 proteins (18008 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski