Capybara microvirus Cap3_SP_668
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W913|A0A4P8W913_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_668 OX=2585489 PE=4 SV=1
MM1 pKa = 7.29 EE2 pKa = 5.49 VINIFQYY9 pKa = 10.78 VPSEE13 pKa = 4.12 GTSFTEE19 pKa = 4.48 PSQCIPDD26 pKa = 3.53 QTLSVQEE33 pKa = 3.79 LLARR37 pKa = 11.84 FSRR40 pKa = 11.84 GYY42 pKa = 10.51 SVDD45 pKa = 3.75 VPIHH49 pKa = 7.26 DD50 pKa = 5.18 DD51 pKa = 3.68 YY52 pKa = 11.96 EE53 pKa = 4.97 SYY55 pKa = 11.03 EE56 pKa = 4.15 DD57 pKa = 4.46 SFEE60 pKa = 3.87 EE61 pKa = 4.65 DD62 pKa = 4.44 AIDD65 pKa = 5.74 GIQDD69 pKa = 3.6 LSDD72 pKa = 3.23 VDD74 pKa = 3.42 ILRR77 pKa = 11.84 EE78 pKa = 3.91 QIHH81 pKa = 5.24 MQKK84 pKa = 10.71 KK85 pKa = 9.0 LAQTSDD91 pKa = 3.27 VMISEE96 pKa = 4.42 VTDD99 pKa = 3.6 KK100 pKa = 10.92 EE101 pKa = 4.15 RR102 pKa = 11.84 SDD104 pKa = 3.97 AKK106 pKa = 11.33 GNDD109 pKa = 3.45 EE110 pKa = 4.67 SSASS114 pKa = 3.49
Molecular weight: 12.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.167
IPC2_protein 3.935
IPC_protein 3.897
Toseland 3.694
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.795
Rodwell 3.732
Grimsley 3.605
Solomon 3.872
Lehninger 3.821
Nozaki 3.999
DTASelect 4.202
Thurlkill 3.745
EMBOSS 3.808
Sillero 4.024
Patrickios 3.439
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.936
Protein with the highest isoelectric point:
>tr|A0A4P8W5F6|A0A4P8W5F6_9VIRU Major capsid protein OS=Capybara microvirus Cap3_SP_668 OX=2585489 PE=3 SV=1
MM1 pKa = 7.39 KK2 pKa = 10.02 CVSPVTIRR10 pKa = 11.84 NPNFGNISKK19 pKa = 10.13 IGYY22 pKa = 8.06 DD23 pKa = 3.02 SMYY26 pKa = 10.49 IGVPCGKK33 pKa = 9.91 CLPCLQNKK41 pKa = 9.26 RR42 pKa = 11.84 NDD44 pKa = 2.8 WYY46 pKa = 11.36 LRR48 pKa = 11.84 LLSEE52 pKa = 4.54 YY53 pKa = 10.32 RR54 pKa = 11.84 SSTNSCFVTLTYY66 pKa = 10.75 SDD68 pKa = 4.56 EE69 pKa = 4.41 NCDD72 pKa = 3.48 GSVHH76 pKa = 6.63 KK77 pKa = 10.69 KK78 pKa = 10.49 DD79 pKa = 3.14 IQEE82 pKa = 3.8 FFARR86 pKa = 11.84 LRR88 pKa = 11.84 KK89 pKa = 9.44 RR90 pKa = 11.84 LQYY93 pKa = 10.93 HH94 pKa = 7.0 LGTVGLRR101 pKa = 11.84 FFCVSEE107 pKa = 4.15 YY108 pKa = 11.02 GPKK111 pKa = 7.62 THH113 pKa = 7.15 RR114 pKa = 11.84 PHH116 pKa = 5.56 YY117 pKa = 7.76 HH118 pKa = 5.32 TLIFGYY124 pKa = 9.17 PDD126 pKa = 3.93 CDD128 pKa = 3.21 INHH131 pKa = 6.67 YY132 pKa = 9.92 IEE134 pKa = 4.58 KK135 pKa = 9.66 SWYY138 pKa = 9.88 LGFVCVTNININRR151 pKa = 11.84 IRR153 pKa = 11.84 YY154 pKa = 6.21 CAKK157 pKa = 10.09 YY158 pKa = 10.05 CISKK162 pKa = 10.73 NDD164 pKa = 3.34 VPEE167 pKa = 4.56 NMDD170 pKa = 4.04 NNFMLCSRR178 pKa = 11.84 RR179 pKa = 11.84 PAIGDD184 pKa = 3.49 SFLTNKK190 pKa = 9.39 VKK192 pKa = 10.5 SFYY195 pKa = 10.62 SKK197 pKa = 9.98 RR198 pKa = 11.84 HH199 pKa = 4.62 GKK201 pKa = 9.75 IIYY204 pKa = 9.1 NDD206 pKa = 3.28 HH207 pKa = 5.83 GVNYY211 pKa = 10.1 SLPRR215 pKa = 11.84 RR216 pKa = 11.84 WKK218 pKa = 10.4 EE219 pKa = 3.53 KK220 pKa = 10.68 LLSEE224 pKa = 4.14 KK225 pKa = 10.39 QKK227 pKa = 11.41 NEE229 pKa = 3.49 IKK231 pKa = 10.13 HH232 pKa = 5.97 NSMIYY237 pKa = 10.96 NNDD240 pKa = 3.43 CYY242 pKa = 11.13 KK243 pKa = 10.74 KK244 pKa = 9.7 YY245 pKa = 9.21 QEE247 pKa = 4.88 KK248 pKa = 9.9 YY249 pKa = 9.31 AAYY252 pKa = 10.22 DD253 pKa = 3.62 EE254 pKa = 4.92 AALAHH259 pKa = 6.48 GNPIMSQLQIDD270 pKa = 3.96 QKK272 pKa = 10.98 LEE274 pKa = 4.05 SMKK277 pKa = 10.67 KK278 pKa = 10.44 YY279 pKa = 10.38 LDD281 pKa = 3.69 KK282 pKa = 10.58 DD283 pKa = 3.87 TKK285 pKa = 10.86 FKK287 pKa = 11.17 LL288 pKa = 3.56
Molecular weight: 33.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.654
IPC2_protein 8.565
IPC_protein 8.404
Toseland 8.799
ProMoST 8.902
Dawson 9.253
Bjellqvist 9.253
Wikipedia 9.414
Rodwell 9.385
Grimsley 9.209
Solomon 9.311
Lehninger 9.268
Nozaki 9.37
DTASelect 9.092
Thurlkill 9.18
EMBOSS 9.385
Sillero 9.385
Patrickios 3.986
IPC_peptide 9.297
IPC2_peptide 8.141
IPC2.peptide.svr19 7.791
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1203
114
516
300.8
34.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.403 ± 0.906
1.413 ± 0.872
5.653 ± 1.094
5.902 ± 0.941
3.99 ± 0.66
5.819 ± 1.029
2.078 ± 0.42
5.902 ± 0.693
6.733 ± 1.097
8.562 ± 1.085
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.159 ± 0.724
5.569 ± 1.077
4.572 ± 0.647
5.653 ± 1.0
4.655 ± 0.443
9.476 ± 0.934
4.406 ± 0.431
4.572 ± 0.459
1.164 ± 0.254
5.32 ± 0.693
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here