Myceliophthora thermophila (strain ATCC 42464 / BCRC 31852 / DSM 1799) (Sporotrichum thermophile)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Sordariomycetidae; Sordariales; Chaetomiaceae; Thermothelomyces; Thermothelomyces thermophilus

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9079 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G2Q0U2|G2Q0U2_MYCTT Uncharacterized protein OS=Myceliophthora thermophila (strain ATCC 42464 / BCRC 31852 / DSM 1799) OX=573729 GN=MYCTH_2294307 PE=4 SV=1
MM1 pKa = 7.62KK2 pKa = 9.1NTAVFVLLGHH12 pKa = 7.21AALAQAEE19 pKa = 4.57AWWEE23 pKa = 3.99GAPEE27 pKa = 3.9CAYY30 pKa = 10.95DD31 pKa = 5.47CFNSWWSSAIAWPAPTSYY49 pKa = 10.92CSANQGASVSNCLRR63 pKa = 11.84TACSATPTAVTSYY76 pKa = 10.99SSLSASLCSRR86 pKa = 11.84WSSCSSAGSTGVYY99 pKa = 9.71TITAPAFTGRR109 pKa = 11.84WPDD112 pKa = 4.39LDD114 pKa = 6.01DD115 pKa = 4.69DD116 pKa = 5.28HH117 pKa = 8.75DD118 pKa = 4.2WDD120 pKa = 5.41DD121 pKa = 5.88LDD123 pKa = 5.38WDD125 pKa = 4.06DD126 pKa = 6.44LDD128 pKa = 5.15WNDD131 pKa = 5.49HH132 pKa = 7.4DD133 pKa = 6.93DD134 pKa = 6.16DD135 pKa = 7.63DD136 pKa = 7.66DD137 pKa = 7.67DD138 pKa = 7.31DD139 pKa = 6.7DD140 pKa = 6.02DD141 pKa = 4.88WDD143 pKa = 4.13DD144 pKa = 4.12LDD146 pKa = 4.17RR147 pKa = 11.84FRR149 pKa = 11.84SRR151 pKa = 11.84WSQFTRR157 pKa = 11.84TWTGGVYY164 pKa = 9.35TVTGCEE170 pKa = 3.51WDD172 pKa = 3.79GNVWAGGPWGYY183 pKa = 11.05GIGGAAGSPWGPWGSGWRR201 pKa = 11.84VTTVTQTITRR211 pKa = 11.84VVTVTSSGGATSLSTSVGPGLVALAVSGDD240 pKa = 3.65VTSTSVVGAAEE251 pKa = 4.18TTGTGTAGNSPSATGGSGGGSVPDD275 pKa = 3.96AAAALAGDD283 pKa = 3.95GALGVKK289 pKa = 9.61VAGAVLGGVIAVAALLL305 pKa = 3.97

Molecular weight: 31.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G2QF51|G2QF51_MYCTT Uncharacterized protein OS=Myceliophthora thermophila (strain ATCC 42464 / BCRC 31852 / DSM 1799) OX=573729 GN=MYCTH_2081554 PE=3 SV=1
MM1 pKa = 7.87PSHH4 pKa = 5.74KK5 pKa = 9.38TFRR8 pKa = 11.84VKK10 pKa = 10.53QKK12 pKa = 9.99LAKK15 pKa = 9.67AQKK18 pKa = 8.59QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.89RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.14WRR45 pKa = 11.84KK46 pKa = 7.51TRR48 pKa = 11.84LGLL51 pKa = 4.09

Molecular weight: 6.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9079

0

9079

4302111

49

9140

473.9

52.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.838 ± 0.024

1.135 ± 0.009

5.691 ± 0.02

6.362 ± 0.029

3.391 ± 0.016

7.546 ± 0.029

2.304 ± 0.011

4.107 ± 0.02

4.475 ± 0.022

8.689 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.97 ± 0.01

3.237 ± 0.014

6.695 ± 0.039

3.925 ± 0.019

6.908 ± 0.025

7.876 ± 0.027

5.731 ± 0.016

6.178 ± 0.02

1.393 ± 0.009

2.549 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski