Limnobacter sp. MED105

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Limnobacter; unclassified Limnobacter

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3244 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A6GMT0|A6GMT0_9BURK YapH protein OS=Limnobacter sp. MED105 OX=391597 GN=LMED105_03025 PE=4 SV=1
MM1 pKa = 7.51AQFDD5 pKa = 4.54PNAPPALFMPPADD18 pKa = 3.9MAHH21 pKa = 7.37DD22 pKa = 4.44IYY24 pKa = 11.47ALSSQLEE31 pKa = 4.43GFDD34 pKa = 3.44HH35 pKa = 7.35NMYY38 pKa = 10.74QDD40 pKa = 3.31IQQDD44 pKa = 3.54QAVYY48 pKa = 10.85DD49 pKa = 3.97SFQRR53 pKa = 11.84WPFLFGIAMASRR65 pKa = 11.84FSEE68 pKa = 4.45DD69 pKa = 2.62

Molecular weight: 7.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A6GL94|A6GL94_9BURK AAA_15 domain-containing protein OS=Limnobacter sp. MED105 OX=391597 GN=LMED105_04482 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.25QPSKK9 pKa = 9.63VRR11 pKa = 11.84RR12 pKa = 11.84QRR14 pKa = 11.84THH16 pKa = 5.6GFLVRR21 pKa = 11.84SRR23 pKa = 11.84TRR25 pKa = 11.84GGRR28 pKa = 11.84AVLRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.48GRR39 pKa = 11.84ARR41 pKa = 11.84LSVV44 pKa = 3.12

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3244

0

3244

1049918

21

3091

323.6

35.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.12 ± 0.045

0.993 ± 0.015

5.012 ± 0.029

5.931 ± 0.038

4.053 ± 0.027

7.681 ± 0.047

2.13 ± 0.026

5.159 ± 0.032

4.819 ± 0.042

10.614 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.621 ± 0.021

3.937 ± 0.036

4.62 ± 0.031

4.438 ± 0.033

5.215 ± 0.038

6.096 ± 0.035

5.307 ± 0.039

7.509 ± 0.036

1.312 ± 0.018

2.432 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski