Limnobacter sp. MED105
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3244 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A6GMT0|A6GMT0_9BURK YapH protein OS=Limnobacter sp. MED105 OX=391597 GN=LMED105_03025 PE=4 SV=1
MM1 pKa = 7.51 AQFDD5 pKa = 4.54 PNAPPALFMPPADD18 pKa = 3.9 MAHH21 pKa = 7.37 DD22 pKa = 4.44 IYY24 pKa = 11.47 ALSSQLEE31 pKa = 4.43 GFDD34 pKa = 3.44 HH35 pKa = 7.35 NMYY38 pKa = 10.74 QDD40 pKa = 3.31 IQQDD44 pKa = 3.54 QAVYY48 pKa = 10.85 DD49 pKa = 3.97 SFQRR53 pKa = 11.84 WPFLFGIAMASRR65 pKa = 11.84 FSEE68 pKa = 4.45 DD69 pKa = 2.62
Molecular weight: 7.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.743
IPC2_protein 3.884
IPC_protein 3.808
Toseland 3.592
ProMoST 3.897
Dawson 3.834
Bjellqvist 4.075
Wikipedia 3.821
Rodwell 3.643
Grimsley 3.503
Solomon 3.795
Lehninger 3.757
Nozaki 3.973
DTASelect 4.24
Thurlkill 3.681
EMBOSS 3.834
Sillero 3.935
Patrickios 1.926
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.894
Protein with the highest isoelectric point:
>tr|A6GL94|A6GL94_9BURK AAA_15 domain-containing protein OS=Limnobacter sp. MED105 OX=391597 GN=LMED105_04482 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.25 QPSKK9 pKa = 9.63 VRR11 pKa = 11.84 RR12 pKa = 11.84 QRR14 pKa = 11.84 THH16 pKa = 5.6 GFLVRR21 pKa = 11.84 SRR23 pKa = 11.84 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVLRR32 pKa = 11.84 ARR34 pKa = 11.84 RR35 pKa = 11.84 SKK37 pKa = 10.48 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSVV44 pKa = 3.12
Molecular weight: 5.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.461
IPC2_protein 11.184
IPC_protein 12.588
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.217
Rodwell 12.34
Grimsley 12.778
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.076
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.116
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3244
0
3244
1049918
21
3091
323.6
35.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.12 ± 0.045
0.993 ± 0.015
5.012 ± 0.029
5.931 ± 0.038
4.053 ± 0.027
7.681 ± 0.047
2.13 ± 0.026
5.159 ± 0.032
4.819 ± 0.042
10.614 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.621 ± 0.021
3.937 ± 0.036
4.62 ± 0.031
4.438 ± 0.033
5.215 ± 0.038
6.096 ± 0.035
5.307 ± 0.039
7.509 ± 0.036
1.312 ± 0.018
2.432 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here