Amino acid dipepetide frequency for Limnobacter sp. MED105

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.653AlaAla: 10.653 ± 0.147	1.228AlaCys: 1.228 ± 0.037	5.038AlaAsp: 5.038 ± 0.072	5.879AlaGlu: 5.879 ± 0.089	3.944AlaPhe: 3.944 ± 0.068	8.037AlaGly: 8.037 ± 0.108	2.015AlaHis: 2.015 ± 0.05	5.219AlaIle: 5.219 ± 0.067	4.906AlaLys: 4.906 ± 0.087	11.457AlaLeu: 11.457 ± 0.119	3.012AlaMet: 3.012 ± 0.065	3.696AlaAsn: 3.696 ± 0.076	3.924AlaPro: 3.924 ± 0.066	4.936AlaGln: 4.936 ± 0.099	5.251AlaArg: 5.251 ± 0.082	5.955AlaSer: 5.955 ± 0.08	4.901AlaThr: 4.901 ± 0.08	7.415AlaVal: 7.415 ± 0.096	1.324AlaTrp: 1.324 ± 0.04	2.412AlaTyr: 2.412 ± 0.052	0.0AlaXaa: 0.0 ± 0.0
Cys	1.04CysAla: 1.04 ± 0.03	0.14CysCys: 0.14 ± 0.012	0.538CysAsp: 0.538 ± 0.021	0.616CysGlu: 0.616 ± 0.024	0.344CysPhe: 0.344 ± 0.017	0.901CysGly: 0.901 ± 0.032	0.277CysHis: 0.277 ± 0.02	0.496CysIle: 0.496 ± 0.02	0.46CysLys: 0.46 ± 0.023	0.949CysLeu: 0.949 ± 0.032	0.265CysMet: 0.265 ± 0.016	0.38CysAsn: 0.38 ± 0.018	0.526CysPro: 0.526 ± 0.027	0.319CysGln: 0.319 ± 0.017	0.424CysArg: 0.424 ± 0.02	0.619CysSer: 0.619 ± 0.023	0.59CysThr: 0.59 ± 0.024	0.732CysVal: 0.732 ± 0.028	0.11CysTrp: 0.11 ± 0.01	0.203CysTyr: 0.203 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	4.644AspAla: 4.644 ± 0.076	0.507AspCys: 0.507 ± 0.022	2.35AspAsp: 2.35 ± 0.06	3.314AspGlu: 3.314 ± 0.056	2.291AspPhe: 2.291 ± 0.048	3.617AspGly: 3.617 ± 0.071	1.084AspHis: 1.084 ± 0.032	2.724AspIle: 2.724 ± 0.058	2.261AspLys: 2.261 ± 0.05	5.533AspLeu: 5.533 ± 0.076	1.318AspMet: 1.318 ± 0.037	1.586AspAsn: 1.586 ± 0.041	2.58AspPro: 2.58 ± 0.054	2.111AspGln: 2.111 ± 0.043	2.649AspArg: 2.649 ± 0.051	3.037AspSer: 3.037 ± 0.062	2.557AspThr: 2.557 ± 0.056	3.622AspVal: 3.622 ± 0.063	0.865AspTrp: 0.865 ± 0.03	1.47AspTyr: 1.47 ± 0.04	0.0AspXaa: 0.0 ± 0.0
Glu	5.579GluAla: 5.579 ± 0.086	0.534GluCys: 0.534 ± 0.024	2.485GluAsp: 2.485 ± 0.053	3.311GluGlu: 3.311 ± 0.07	2.61GluPhe: 2.61 ± 0.054	4.025GluGly: 4.025 ± 0.072	1.422GluHis: 1.422 ± 0.042	3.576GluIle: 3.576 ± 0.056	3.262GluLys: 3.262 ± 0.053	6.743GluLeu: 6.743 ± 0.091	1.565GluMet: 1.565 ± 0.044	2.355GluAsn: 2.355 ± 0.047	2.257GluPro: 2.257 ± 0.051	3.258GluGln: 3.258 ± 0.058	3.423GluArg: 3.423 ± 0.065	3.398GluSer: 3.398 ± 0.058	3.039GluThr: 3.039 ± 0.057	4.397GluVal: 4.397 ± 0.075	0.7GluTrp: 0.7 ± 0.027	1.369GluTyr: 1.369 ± 0.038	0.0GluXaa: 0.0 ± 0.0
Phe	3.979PheAla: 3.979 ± 0.067	0.407PheCys: 0.407 ± 0.021	2.71PheAsp: 2.71 ± 0.053	2.86PheGlu: 2.86 ± 0.054	1.627PhePhe: 1.627 ± 0.056	3.36PheGly: 3.36 ± 0.061	0.745PheHis: 0.745 ± 0.026	1.9PheIle: 1.9 ± 0.049	2.008PheLys: 2.008 ± 0.045	3.573PheLeu: 3.573 ± 0.074	1.045PheMet: 1.045 ± 0.035	1.754PheAsn: 1.754 ± 0.042	1.618PhePro: 1.618 ± 0.037	1.456PheGln: 1.456 ± 0.038	1.787PheArg: 1.787 ± 0.047	2.939PheSer: 2.939 ± 0.06	2.184PheThr: 2.184 ± 0.045	2.977PheVal: 2.977 ± 0.061	0.535PheTrp: 0.535 ± 0.027	1.061PheTyr: 1.061 ± 0.032	0.0PheXaa: 0.0 ± 0.0
Gly	6.806GlyAla: 6.806 ± 0.101	0.858GlyCys: 0.858 ± 0.031	3.646GlyAsp: 3.646 ± 0.076	4.437GlyGlu: 4.437 ± 0.068	3.656GlyPhe: 3.656 ± 0.073	6.031GlyGly: 6.031 ± 0.103	1.638GlyHis: 1.638 ± 0.043	4.164GlyIle: 4.164 ± 0.068	4.199GlyLys: 4.199 ± 0.065	8.748GlyLeu: 8.748 ± 0.114	2.188GlyMet: 2.188 ± 0.05	2.758GlyAsn: 2.758 ± 0.077	2.417GlyPro: 2.417 ± 0.054	3.539GlyGln: 3.539 ± 0.067	3.673GlyArg: 3.673 ± 0.062	4.53GlySer: 4.53 ± 0.076	4.023GlyThr: 4.023 ± 0.085	6.231GlyVal: 6.231 ± 0.091	1.023GlyTrp: 1.023 ± 0.037	2.246GlyTyr: 2.246 ± 0.058	0.0GlyXaa: 0.0 ± 0.0
His	2.174HisAla: 2.174 ± 0.054	0.31HisCys: 0.31 ± 0.018	1.052HisAsp: 1.052 ± 0.037	1.137HisGlu: 1.137 ± 0.034	0.949HisPhe: 0.949 ± 0.026	1.794HisGly: 1.794 ± 0.046	0.632HisHis: 0.632 ± 0.032	1.017HisIle: 1.017 ± 0.033	0.811HisLys: 0.811 ± 0.029	2.235HisLeu: 2.235 ± 0.048	0.535HisMet: 0.535 ± 0.022	0.691HisAsn: 0.691 ± 0.028	1.208HisPro: 1.208 ± 0.038	0.844HisGln: 0.844 ± 0.029	1.058HisArg: 1.058 ± 0.036	1.223HisSer: 1.223 ± 0.031	1.198HisThr: 1.198 ± 0.04	1.452HisVal: 1.452 ± 0.04	0.374HisTrp: 0.374 ± 0.02	0.604HisTyr: 0.604 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	5.864IleAla: 5.864 ± 0.08	0.492IleCys: 0.492 ± 0.021	3.356IleAsp: 3.356 ± 0.056	4.131IleGlu: 4.131 ± 0.062	1.589IlePhe: 1.589 ± 0.038	4.044IleGly: 4.044 ± 0.073	1.143IleHis: 1.143 ± 0.034	2.005IleIle: 2.005 ± 0.045	2.394IleLys: 2.394 ± 0.045	4.27IleLeu: 4.27 ± 0.074	1.037IleMet: 1.037 ± 0.029	2.098IleAsn: 2.098 ± 0.047	2.454IlePro: 2.454 ± 0.053	2.354IleGln: 2.354 ± 0.042	2.895IleArg: 2.895 ± 0.057	3.266IleSer: 3.266 ± 0.063	2.926IleThr: 2.926 ± 0.065	3.611IleVal: 3.611 ± 0.066	0.541IleTrp: 0.541 ± 0.025	1.119IleTyr: 1.119 ± 0.036	0.0IleXaa: 0.0 ± 0.0
Lys	5.521LysAla: 5.521 ± 0.097	0.272LysCys: 0.272 ± 0.016	2.107LysAsp: 2.107 ± 0.047	2.495LysGlu: 2.495 ± 0.054	1.544LysPhe: 1.544 ± 0.046	3.457LysGly: 3.457 ± 0.079	1.059LysHis: 1.059 ± 0.03	2.351LysIle: 2.351 ± 0.052	2.576LysLys: 2.576 ± 0.054	4.904LysLeu: 4.904 ± 0.073	1.327LysMet: 1.327 ± 0.035	1.928LysAsn: 1.928 ± 0.041	3.016LysPro: 3.016 ± 0.066	2.21LysGln: 2.21 ± 0.045	2.699LysArg: 2.699 ± 0.057	2.732LysSer: 2.732 ± 0.054	2.873LysThr: 2.873 ± 0.056	3.547LysVal: 3.547 ± 0.064	0.535LysTrp: 0.535 ± 0.024	1.032LysTyr: 1.032 ± 0.036	0.0LysXaa: 0.0 ± 0.0
Leu	11.34LeuAla: 11.34 ± 0.112	1.095LeuCys: 1.095 ± 0.039	5.506LeuAsp: 5.506 ± 0.082	6.081LeuGlu: 6.081 ± 0.083	3.913LeuPhe: 3.913 ± 0.076	8.407LeuGly: 8.407 ± 0.12	2.077LeuHis: 2.077 ± 0.046	5.541LeuIle: 5.541 ± 0.093	5.366LeuLys: 5.366 ± 0.081	10.628LeuLeu: 10.628 ± 0.143	2.67LeuMet: 2.67 ± 0.071	4.887LeuAsn: 4.887 ± 0.082	5.495LeuPro: 5.495 ± 0.077	4.433LeuGln: 4.433 ± 0.076	5.654LeuArg: 5.654 ± 0.081	7.052LeuSer: 7.052 ± 0.099	5.287LeuThr: 5.287 ± 0.086	7.36LeuVal: 7.36 ± 0.102	1.176LeuTrp: 1.176 ± 0.036	2.167LeuTyr: 2.167 ± 0.056	0.0LeuXaa: 0.0 ± 0.0
Met	2.827MetAla: 2.827 ± 0.057	0.205MetCys: 0.205 ± 0.013	1.193MetAsp: 1.193 ± 0.035	1.262MetGlu: 1.262 ± 0.037	0.906MetPhe: 0.906 ± 0.03	2.104MetGly: 2.104 ± 0.048	0.583MetHis: 0.583 ± 0.02	1.286MetIle: 1.286 ± 0.035	1.447MetLys: 1.447 ± 0.037	2.771MetLeu: 2.771 ± 0.073	0.679MetMet: 0.679 ± 0.029	1.259MetAsn: 1.259 ± 0.037	1.447MetPro: 1.447 ± 0.035	1.192MetGln: 1.192 ± 0.035	1.426MetArg: 1.426 ± 0.037	1.654MetSer: 1.654 ± 0.039	1.393MetThr: 1.393 ± 0.036	1.891MetVal: 1.891 ± 0.041	0.242MetTrp: 0.242 ± 0.015	0.446MetTyr: 0.446 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	3.912AsnAla: 3.912 ± 0.077	0.417AsnCys: 0.417 ± 0.021	1.738AsnAsp: 1.738 ± 0.043	1.973AsnGlu: 1.973 ± 0.047	1.449AsnPhe: 1.449 ± 0.038	2.901AsnGly: 2.901 ± 0.065	0.793AsnHis: 0.793 ± 0.026	2.042AsnIle: 2.042 ± 0.046	1.709AsnLys: 1.709 ± 0.043	4.042AsnLeu: 4.042 ± 0.072	0.975AsnMet: 0.975 ± 0.028	1.646AsnAsn: 1.646 ± 0.055	2.534AsnPro: 2.534 ± 0.056	1.733AsnGln: 1.733 ± 0.043	2.124AsnArg: 2.124 ± 0.044	2.497AsnSer: 2.497 ± 0.052	2.594AsnThr: 2.594 ± 0.094	2.635AsnVal: 2.635 ± 0.071	0.616AsnTrp: 0.616 ± 0.026	1.042AsnTyr: 1.042 ± 0.038	0.0AsnXaa: 0.0 ± 0.0
Pro	4.823ProAla: 4.823 ± 0.079	0.36ProCys: 0.36 ± 0.017	2.629ProAsp: 2.629 ± 0.051	3.449ProGlu: 3.449 ± 0.063	1.913ProPhe: 1.913 ± 0.045	3.609ProGly: 3.609 ± 0.061	0.893ProHis: 0.893 ± 0.033	2.217ProIle: 2.217 ± 0.05	2.198ProLys: 2.198 ± 0.049	4.546ProLeu: 4.546 ± 0.076	1.232ProMet: 1.232 ± 0.036	1.933ProAsn: 1.933 ± 0.049	1.703ProPro: 1.703 ± 0.043	1.787ProGln: 1.787 ± 0.042	1.878ProArg: 1.878 ± 0.044	2.674ProSer: 2.674 ± 0.05	2.506ProThr: 2.506 ± 0.048	4.109ProVal: 4.109 ± 0.068	0.627ProTrp: 0.627 ± 0.028	1.113ProTyr: 1.113 ± 0.038	0.0ProXaa: 0.0 ± 0.0
Gln	5.381GlnAla: 5.381 ± 0.099	0.379GlnCys: 0.379 ± 0.021	1.611GlnAsp: 1.611 ± 0.04	2.135GlnGlu: 2.135 ± 0.047	1.89GlnPhe: 1.89 ± 0.043	3.377GlnGly: 3.377 ± 0.058	1.079GlnHis: 1.079 ± 0.039	2.409GlnIle: 2.409 ± 0.048	1.783GlnLys: 1.783 ± 0.042	4.621GlnLeu: 4.621 ± 0.076	1.123GlnMet: 1.123 ± 0.039	1.534GlnAsn: 1.534 ± 0.041	1.864GlnPro: 1.864 ± 0.045	2.378GlnGln: 2.378 ± 0.053	2.499GlnArg: 2.499 ± 0.056	2.636GlnSer: 2.636 ± 0.054	2.447GlnThr: 2.447 ± 0.056	3.427GlnVal: 3.427 ± 0.066	0.711GlnTrp: 0.711 ± 0.027	1.095GlnTyr: 1.095 ± 0.033	0.0GlnXaa: 0.0 ± 0.0
Arg	4.71ArgAla: 4.71 ± 0.066	0.48ArgCys: 0.48 ± 0.021	2.622ArgAsp: 2.622 ± 0.052	3.322ArgGlu: 3.322 ± 0.059	2.419ArgPhe: 2.419 ± 0.044	3.124ArgGly: 3.124 ± 0.064	1.145ArgHis: 1.145 ± 0.032	3.114ArgIle: 3.114 ± 0.05	2.588ArgLys: 2.588 ± 0.048	5.968ArgLeu: 5.968 ± 0.089	1.586ArgMet: 1.586 ± 0.036	2.067ArgAsn: 2.067 ± 0.048	2.109ArgPro: 2.109 ± 0.05	2.284ArgGln: 2.284 ± 0.054	2.828ArgArg: 2.828 ± 0.048	2.865ArgSer: 2.865 ± 0.055	2.553ArgThr: 2.553 ± 0.053	4.081ArgVal: 4.081 ± 0.065	0.778ArgTrp: 0.778 ± 0.028	1.512ArgTyr: 1.512 ± 0.041	0.0ArgXaa: 0.0 ± 0.0
Ser	5.907SerAla: 5.907 ± 0.079	0.528SerCys: 0.528 ± 0.026	3.081SerAsp: 3.081 ± 0.084	3.539SerGlu: 3.539 ± 0.063	2.414SerPhe: 2.414 ± 0.051	5.048SerGly: 5.048 ± 0.082	1.205SerHis: 1.205 ± 0.035	3.315SerIle: 3.315 ± 0.055	3.03SerLys: 3.03 ± 0.058	6.218SerLeu: 6.218 ± 0.099	1.619SerMet: 1.619 ± 0.046	2.544SerAsn: 2.544 ± 0.066	2.843SerPro: 2.843 ± 0.057	2.385SerGln: 2.385 ± 0.05	3.191SerArg: 3.191 ± 0.06	3.978SerSer: 3.978 ± 0.091	3.635SerThr: 3.635 ± 0.07	4.355SerVal: 4.355 ± 0.072	0.734SerTrp: 0.734 ± 0.026	1.397SerTyr: 1.397 ± 0.038	0.0SerXaa: 0.0 ± 0.0
Thr	5.222ThrAla: 5.222 ± 0.09	0.467ThrCys: 0.467 ± 0.023	2.666ThrAsp: 2.666 ± 0.06	3.031ThrGlu: 3.031 ± 0.052	2.005ThrPhe: 2.005 ± 0.05	4.71ThrGly: 4.71 ± 0.112	1.199ThrHis: 1.199 ± 0.041	2.615ThrIle: 2.615 ± 0.051	1.974ThrLys: 1.974 ± 0.051	6.171ThrLeu: 6.171 ± 0.081	1.164ThrMet: 1.164 ± 0.035	1.814ThrAsn: 1.814 ± 0.053	3.156ThrPro: 3.156 ± 0.059	2.343ThrGln: 2.343 ± 0.044	2.735ThrArg: 2.735 ± 0.056	2.893ThrSer: 2.893 ± 0.059	2.811ThrThr: 2.811 ± 0.068	4.228ThrVal: 4.228 ± 0.07	0.653ThrTrp: 0.653 ± 0.022	1.215ThrTyr: 1.215 ± 0.04	0.0ThrXaa: 0.0 ± 0.0
Val	7.418ValAla: 7.418 ± 0.098	0.825ValCys: 0.825 ± 0.034	3.992ValAsp: 3.992 ± 0.063	4.476ValGlu: 4.476 ± 0.086	3.235ValPhe: 3.235 ± 0.055	5.414ValGly: 5.414 ± 0.081	1.532ValHis: 1.532 ± 0.036	3.788ValIle: 3.788 ± 0.071	3.54ValLys: 3.54 ± 0.062	8.379ValLeu: 8.379 ± 0.106	1.953ValMet: 1.953 ± 0.046	3.062ValAsn: 3.062 ± 0.061	3.338ValPro: 3.338 ± 0.061	3.125ValGln: 3.125 ± 0.064	3.77ValArg: 3.77 ± 0.067	4.698ValSer: 4.698 ± 0.076	3.547ValThr: 3.547 ± 0.065	6.178ValVal: 6.178 ± 0.076	0.99ValTrp: 0.99 ± 0.033	1.833ValTyr: 1.833 ± 0.042	0.0ValXaa: 0.0 ± 0.0
Trp	1.176TrpAla: 1.176 ± 0.038	0.13TrpCys: 0.13 ± 0.011	0.536TrpAsp: 0.536 ± 0.025	0.583TrpGlu: 0.583 ± 0.027	0.608TrpPhe: 0.608 ± 0.024	0.867TrpGly: 0.867 ± 0.031	0.299TrpHis: 0.299 ± 0.016	0.744TrpIle: 0.744 ± 0.029	0.577TrpLys: 0.577 ± 0.025	1.805TrpLeu: 1.805 ± 0.057	0.389TrpMet: 0.389 ± 0.021	0.531TrpAsn: 0.531 ± 0.024	0.571TrpPro: 0.571 ± 0.027	0.647TrpGln: 0.647 ± 0.026	0.717TrpArg: 0.717 ± 0.026	0.641TrpSer: 0.641 ± 0.024	0.616TrpThr: 0.616 ± 0.025	1.162TrpVal: 1.162 ± 0.034	0.197TrpTrp: 0.197 ± 0.015	0.323TrpTyr: 0.323 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.221TyrAla: 2.221 ± 0.055	0.295TyrCys: 0.295 ± 0.015	1.263TyrAsp: 1.263 ± 0.038	1.279TyrGlu: 1.279 ± 0.034	1.223TyrPhe: 1.223 ± 0.038	1.984TyrGly: 1.984 ± 0.053	0.479TyrHis: 0.479 ± 0.023	1.067TyrIle: 1.067 ± 0.033	1.096TyrLys: 1.096 ± 0.038	2.574TyrLeu: 2.574 ± 0.051	0.531TyrMet: 0.531 ± 0.025	0.847TyrAsn: 0.847 ± 0.039	1.14TyrPro: 1.14 ± 0.038	1.049TyrGln: 1.049 ± 0.034	1.473TyrArg: 1.473 ± 0.04	1.676TyrSer: 1.676 ± 0.038	1.394TyrThr: 1.394 ± 0.045	1.681TyrVal: 1.681 ± 0.047	0.385TyrTrp: 0.385 ± 0.017	0.666TyrTyr: 0.666 ± 0.028	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3244 proteins (1049918 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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