Circoviridae 10 LDMD-2013

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; environmental samples

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|S5T687|S5T687_9CIRC Uncharacterized protein OS=Circoviridae 10 LDMD-2013 OX=1379714 PE=4 SV=1
MM1 pKa = 8.1DD2 pKa = 5.99PGDD5 pKa = 4.43PDD7 pKa = 3.72FDD9 pKa = 4.91AYY11 pKa = 10.77AGLPIQANVGSPAEE25 pKa = 4.11PADD28 pKa = 3.35PHH30 pKa = 7.19NLVNQGVRR38 pKa = 11.84ALILAGGEE46 pKa = 4.08LVRR49 pKa = 11.84QYY51 pKa = 11.72LFGRR55 pKa = 11.84EE56 pKa = 4.0LLPAEE61 pKa = 4.68RR62 pKa = 11.84LIEE65 pKa = 4.59DD66 pKa = 4.93PPPPPYY72 pKa = 10.57DD73 pKa = 4.03PSDD76 pKa = 3.78SDD78 pKa = 3.95SSSSSSEE85 pKa = 4.1DD86 pKa = 3.19EE87 pKa = 4.35GPYY90 pKa = 10.22PLNTTDD96 pKa = 3.91QIGYY100 pKa = 9.22KK101 pKa = 9.52YY102 pKa = 10.32AGRR105 pKa = 11.84APTVEE110 pKa = 4.02QYY112 pKa = 10.88LARR115 pKa = 11.84NVQKK119 pKa = 9.87TSS121 pKa = 2.82

Molecular weight:
13.03 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|S5TND6|S5TND6_9CIRC ATP-dependent helicase Rep (Fragment) OS=Circoviridae 10 LDMD-2013 OX=1379714 PE=3 SV=1
KKK2 pKa = 9.92DD3 pKa = 3.5YY4 pKa = 9.32KKK6 pKa = 10.54KK7 pKa = 10.53PGPALGTKKK16 pKa = 9.63YYY18 pKa = 9.56NWIFTSYYY26 pKa = 11.11DD27 pKa = 3.48DDD29 pKa = 3.43NPYYY33 pKa = 8.35KK34 pKa = 10.36FSGSPWAQYYY44 pKa = 9.27ICQAEEE50 pKa = 4.26KK51 pKa = 10.76PTTGRR56 pKa = 11.84VHHH59 pKa = 5.01QGYYY63 pKa = 6.08HHH65 pKa = 6.98NTPVGLATLQRR76 pKa = 11.84EEE78 pKa = 4.13GLPGVHHH85 pKa = 6.55EEE87 pKa = 4.12RR88 pKa = 11.84RR89 pKa = 11.84GNHHH93 pKa = 5.35KK94 pKa = 9.09EE95 pKa = 3.27RR96 pKa = 11.84QYYY99 pKa = 10.42RR100 pKa = 11.84KKK102 pKa = 9.9EE103 pKa = 3.93EE104 pKa = 4.03RR105 pKa = 11.84VPGSLPIEEE114 pKa = 4.67GDDD117 pKa = 3.95PQQGSRR123 pKa = 11.84TDDD126 pKa = 4.3DDD128 pKa = 3.73DD129 pKa = 4.61KKK131 pKa = 10.89ILVEEE136 pKa = 4.33GLLGAFEEE144 pKa = 5.0DDD146 pKa = 3.56SSTVRR151 pKa = 11.84FYYY154 pKa = 11.21GFEEE158 pKa = 4.09YYY160 pKa = 9.26SLVGQPRR167 pKa = 11.84TNDDD171 pKa = 3.34AQEEE175 pKa = 3.86YYY177 pKa = 10.59YY178 pKa = 10.36YYY180 pKa = 10.96PPGTGKKK187 pKa = 7.98RR188 pKa = 11.84AVWEEE193 pKa = 4.44VPDDD197 pKa = 4.0DDD199 pKa = 5.45VYYY202 pKa = 11.24CPTSSGKKK210 pKa = 9.43WFDDD214 pKa = 3.54YYY216 pKa = 11.18PGYYY220 pKa = 8.05HH221 pKa = 5.29VILVDDD227 pKa = 4.56DD228 pKa = 4.81YY229 pKa = 11.3GQWPVPFFLQFLDDD243 pKa = 3.84YYY245 pKa = 10.6KK246 pKa = 11.13LLPVKKK252 pKa = 9.95SHHH255 pKa = 5.24HHH257 pKa = 5.57GRR259 pKa = 11.84AKKK262 pKa = 10.14YYY264 pKa = 7.8TSNEEE269 pKa = 4.33IEEE272 pKa = 4.62IYYY275 pKa = 11.26DDD277 pKa = 3.68PAVTIAAIRR286 pKa = 11.84RR287 pKa = 11.84RR288 pKa = 11.84FKKK291 pKa = 10.41EE292 pKa = 3.6KKK294 pKa = 10.23HH295 pKa = 5.91NTL

Molecular weight:
34.15 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

786

121

367

262.0

29.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.489 ± 0.835

0.763 ± 0.308

6.489 ± 0.835

5.344 ± 0.388

4.326 ± 0.875

8.906 ± 0.26

2.163 ± 0.422

4.326 ± 0.33

4.962 ± 1.033

6.616 ± 0.92

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.272 ± 0.547

4.326 ± 1.029

8.142 ± 1.727

3.308 ± 0.76

5.344 ± 1.503

7.506 ± 1.352

5.98 ± 0.829

6.489 ± 0.529

1.527 ± 0.616

5.598 ± 0.422

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski