Circoviridae 10 LDMD-2013
Average proteome isoelectric point is 5.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S5T687|S5T687_9CIRC Uncharacterized protein OS=Circoviridae 10 LDMD-2013 OX=1379714 PE=4 SV=1
MM1 pKa = 8.1 DD2 pKa = 5.99 PGDD5 pKa = 4.43 PDD7 pKa = 3.72 FDD9 pKa = 4.91 AYY11 pKa = 10.77 AGLPIQANVGSPAEE25 pKa = 4.11 PADD28 pKa = 3.35 PHH30 pKa = 7.19 NLVNQGVRR38 pKa = 11.84 ALILAGGEE46 pKa = 4.08 LVRR49 pKa = 11.84 QYY51 pKa = 11.72 LFGRR55 pKa = 11.84 EE56 pKa = 4.0 LLPAEE61 pKa = 4.68 RR62 pKa = 11.84 LIEE65 pKa = 4.59 DD66 pKa = 4.93 PPPPPYY72 pKa = 10.57 DD73 pKa = 4.03 PSDD76 pKa = 3.78 SDD78 pKa = 3.95 SSSSSSEE85 pKa = 4.1 DD86 pKa = 3.19 EE87 pKa = 4.35 GPYY90 pKa = 10.22 PLNTTDD96 pKa = 3.91 QIGYY100 pKa = 9.22 KK101 pKa = 9.52 YY102 pKa = 10.32 AGRR105 pKa = 11.84 APTVEE110 pKa = 4.02 QYY112 pKa = 10.88 LARR115 pKa = 11.84 NVQKK119 pKa = 9.87 TSS121 pKa = 2.82
Molecular weight: 13.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.133
IPC2_protein 4.113
IPC_protein 4.05
Toseland 3.846
ProMoST 4.202
Dawson 4.024
Bjellqvist 4.19
Wikipedia 3.961
Rodwell 3.884
Grimsley 3.757
Solomon 4.024
Lehninger 3.973
Nozaki 4.151
DTASelect 4.355
Thurlkill 3.897
EMBOSS 3.961
Sillero 4.164
Patrickios 3.249
IPC_peptide 4.024
IPC2_peptide 4.151
IPC2.peptide.svr19 4.089
Protein with the highest isoelectric point:
>tr|S5TND6|S5TND6_9CIRC ATP-dependent helicase Rep (Fragment) OS=Circoviridae 10 LDMD-2013 OX=1379714 PE=3 SV=1
KKK2 pKa = 9.92 DD3 pKa = 3.5 YY4 pKa = 9.32 KKK6 pKa = 10.54 KK7 pKa = 10.53 PGPALGTKKK16 pKa = 9.63 YYY18 pKa = 9.56 NWIFTSYYY26 pKa = 11.11 DD27 pKa = 3.48 DDD29 pKa = 3.43 NPYYY33 pKa = 8.35 KK34 pKa = 10.36 FSGSPWAQYYY44 pKa = 9.27 ICQAEEE50 pKa = 4.26 KK51 pKa = 10.76 PTTGRR56 pKa = 11.84 VHHH59 pKa = 5.01 QGYYY63 pKa = 6.08 HHH65 pKa = 6.98 NTPVGLATLQRR76 pKa = 11.84 EEE78 pKa = 4.13 GLPGVHHH85 pKa = 6.55 EEE87 pKa = 4.12 RR88 pKa = 11.84 RR89 pKa = 11.84 GNHHH93 pKa = 5.35 KK94 pKa = 9.09 EE95 pKa = 3.27 RR96 pKa = 11.84 QYYY99 pKa = 10.42 RR100 pKa = 11.84 KKK102 pKa = 9.9 EE103 pKa = 3.93 EE104 pKa = 4.03 RR105 pKa = 11.84 VPGSLPIEEE114 pKa = 4.67 GDDD117 pKa = 3.95 PQQGSRR123 pKa = 11.84 TDDD126 pKa = 4.3 DDD128 pKa = 3.73 DD129 pKa = 4.61 KKK131 pKa = 10.89 ILVEEE136 pKa = 4.33 GLLGAFEEE144 pKa = 5.0 DDD146 pKa = 3.56 SSTVRR151 pKa = 11.84 FYYY154 pKa = 11.21 GFEEE158 pKa = 4.09 YYY160 pKa = 9.26 SLVGQPRR167 pKa = 11.84 TNDDD171 pKa = 3.34 AQEEE175 pKa = 3.86 YYY177 pKa = 10.59 YY178 pKa = 10.36 YYY180 pKa = 10.96 PPGTGKKK187 pKa = 7.98 RR188 pKa = 11.84 AVWEEE193 pKa = 4.44 VPDDD197 pKa = 4.0 DDD199 pKa = 5.45 VYYY202 pKa = 11.24 CPTSSGKKK210 pKa = 9.43 WFDDD214 pKa = 3.54 YYY216 pKa = 11.18 PGYYY220 pKa = 8.05 HH221 pKa = 5.29 VILVDDD227 pKa = 4.56 DD228 pKa = 4.81 YY229 pKa = 11.3 GQWPVPFFLQFLDDD243 pKa = 3.84 YYY245 pKa = 10.6 KK246 pKa = 11.13 LLPVKKK252 pKa = 9.95 SHHH255 pKa = 5.24 HHH257 pKa = 5.57 GRR259 pKa = 11.84 AKKK262 pKa = 10.14 YYY264 pKa = 7.8 TSNEEE269 pKa = 4.33 IEEE272 pKa = 4.62 IYYY275 pKa = 11.26 DDD277 pKa = 3.68 PAVTIAAIRR286 pKa = 11.84 RR287 pKa = 11.84 RR288 pKa = 11.84 FKKK291 pKa = 10.41 EE292 pKa = 3.6 KKK294 pKa = 10.23 HH295 pKa = 5.91 NTL
Molecular weight: 34.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.766
IPC2_protein 8.829
IPC_protein 8.799
Toseland 9.107
ProMoST 9.165
Dawson 9.502
Bjellqvist 9.341
Wikipedia 9.721
Rodwell 9.589
Grimsley 9.604
Solomon 9.545
Lehninger 9.487
Nozaki 9.297
DTASelect 9.282
Thurlkill 9.311
EMBOSS 9.589
Sillero 9.472
Patrickios 4.368
IPC_peptide 9.531
IPC2_peptide 8.126
IPC2.peptide.svr19 7.813
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
786
121
367
262.0
29.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.489 ± 0.835
0.763 ± 0.308
6.489 ± 0.835
5.344 ± 0.388
4.326 ± 0.875
8.906 ± 0.26
2.163 ± 0.422
4.326 ± 0.33
4.962 ± 1.033
6.616 ± 0.92
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.272 ± 0.547
4.326 ± 1.029
8.142 ± 1.727
3.308 ± 0.76
5.344 ± 1.503
7.506 ± 1.352
5.98 ± 0.829
6.489 ± 0.529
1.527 ± 0.616
5.598 ± 0.422
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here