Mycoplasma pullorum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasmopsis

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 701 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L4FSJ7|A0A1L4FSJ7_9MOLU Uncharacterized protein OS=Mycoplasma pullorum OX=48003 GN=BLA55_02750 PE=4 SV=1
MM1 pKa = 7.55YY2 pKa = 10.12KK3 pKa = 10.09RR4 pKa = 11.84QVVDD8 pKa = 2.9LHH10 pKa = 6.88GYY12 pKa = 9.55QSDD15 pKa = 4.08EE16 pKa = 3.92IVGIIYY22 pKa = 9.89NLIYY26 pKa = 10.36EE27 pKa = 4.18FHH29 pKa = 7.04NDD31 pKa = 3.03GNLEE35 pKa = 4.01LLFITGHH42 pKa = 5.41GRR44 pKa = 11.84GAVKK48 pKa = 9.26YY49 pKa = 7.91TLLNILEE56 pKa = 4.41KK57 pKa = 10.94EE58 pKa = 4.12KK59 pKa = 10.43IDD61 pKa = 3.68YY62 pKa = 10.86QEE64 pKa = 4.25INQGGAFLIKK74 pKa = 9.28PQIYY78 pKa = 10.03SSYY81 pKa = 11.42NFADD85 pKa = 3.5WDD87 pKa = 5.46DD88 pKa = 3.71EE89 pKa = 4.87DD90 pKa = 4.53EE91 pKa = 4.1WDD93 pKa = 4.0EE94 pKa = 5.07QISQNDD100 pKa = 3.37LDD102 pKa = 4.62VIFNEE107 pKa = 3.93YY108 pKa = 10.79LKK110 pKa = 10.21TKK112 pKa = 9.39EE113 pKa = 3.84

Molecular weight: 13.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L4FS38|A0A1L4FS38_9MOLU 30S ribosomal protein S14 type Z OS=Mycoplasma pullorum OX=48003 GN=rpsN PE=3 SV=1
MM1 pKa = 7.34ARR3 pKa = 11.84KK4 pKa = 9.46ALIEE8 pKa = 3.93KK9 pKa = 10.2AKK11 pKa = 10.03RR12 pKa = 11.84HH13 pKa = 5.81PKK15 pKa = 10.03FSTRR19 pKa = 11.84AYY21 pKa = 8.48TRR23 pKa = 11.84CEE25 pKa = 3.8LCGRR29 pKa = 11.84PHH31 pKa = 7.07AVLRR35 pKa = 11.84KK36 pKa = 9.32YY37 pKa = 9.94KK38 pKa = 10.01VCRR41 pKa = 11.84ICFRR45 pKa = 11.84NLAHH49 pKa = 6.85EE50 pKa = 4.47GKK52 pKa = 10.15IPGMKK57 pKa = 9.27KK58 pKa = 10.56ASWW61 pKa = 3.19

Molecular weight: 7.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

701

0

701

275436

37

5949

392.9

44.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.655 ± 0.15

0.457 ± 0.025

5.863 ± 0.108

6.977 ± 0.089

5.283 ± 0.116

4.43 ± 0.113

1.425 ± 0.043

8.877 ± 0.126

9.583 ± 0.106

9.604 ± 0.079

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.651 ± 0.055

7.978 ± 0.156

2.653 ± 0.059

3.773 ± 0.088

2.975 ± 0.057

6.757 ± 0.077

5.219 ± 0.094

5.768 ± 0.093

0.997 ± 0.027

4.076 ± 0.077
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski