Amino acid dipepetide frequency for Mycoplasma pullorum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.507AlaAla: 3.507 ± 0.2
0.301AlaCys: 0.301 ± 0.034
2.77AlaAsp: 2.77 ± 0.17
3.395AlaGlu: 3.395 ± 0.142
2.501AlaPhe: 2.501 ± 0.099
2.752AlaGly: 2.752 ± 0.135
0.89AlaHis: 0.89 ± 0.057
5.286AlaIle: 5.286 ± 0.179
6.259AlaLys: 6.259 ± 0.308
5.627AlaLeu: 5.627 ± 0.215
0.958AlaMet: 0.958 ± 0.076
4.487AlaAsn: 4.487 ± 0.21
1.38AlaPro: 1.38 ± 0.074
2.992AlaGln: 2.992 ± 0.148
1.855AlaArg: 1.855 ± 0.079
3.362AlaSer: 3.362 ± 0.146
3.115AlaThr: 3.115 ± 0.139
2.669AlaVal: 2.669 ± 0.107
0.418AlaTrp: 0.418 ± 0.041
2.03AlaTyr: 2.03 ± 0.089
0.0AlaXaa: 0.0 ± 0.0
Cys
0.298CysAla: 0.298 ± 0.037
0.04CysCys: 0.04 ± 0.012
0.341CysAsp: 0.341 ± 0.041
0.323CysGlu: 0.323 ± 0.04
0.309CysPhe: 0.309 ± 0.033
0.33CysGly: 0.33 ± 0.044
0.087CysHis: 0.087 ± 0.017
0.367CysIle: 0.367 ± 0.039
0.258CysLys: 0.258 ± 0.03
0.487CysLeu: 0.487 ± 0.045
0.084CysMet: 0.084 ± 0.018
0.196CysAsn: 0.196 ± 0.027
0.171CysPro: 0.171 ± 0.025
0.145CysGln: 0.145 ± 0.027
0.16CysArg: 0.16 ± 0.025
0.265CysSer: 0.265 ± 0.037
0.163CysThr: 0.163 ± 0.025
0.345CysVal: 0.345 ± 0.041
0.047CysTrp: 0.047 ± 0.015
0.152CysTyr: 0.152 ± 0.027
0.0CysXaa: 0.0 ± 0.0
Asp
3.565AspAla: 3.565 ± 0.244
0.218AspCys: 0.218 ± 0.03
2.984AspAsp: 2.984 ± 0.106
4.723AspGlu: 4.723 ± 0.166
3.729AspPhe: 3.729 ± 0.158
2.69AspGly: 2.69 ± 0.128
0.752AspHis: 0.752 ± 0.053
4.291AspIle: 4.291 ± 0.136
5.489AspLys: 5.489 ± 0.193
6.408AspLeu: 6.408 ± 0.198
0.661AspMet: 0.661 ± 0.047
3.798AspAsn: 3.798 ± 0.149
1.746AspPro: 1.746 ± 0.085
2.4AspGln: 2.4 ± 0.157
1.543AspArg: 1.543 ± 0.097
3.848AspSer: 3.848 ± 0.137
2.836AspThr: 2.836 ± 0.117
3.42AspVal: 3.42 ± 0.116
0.603AspTrp: 0.603 ± 0.047
2.923AspTyr: 2.923 ± 0.101
0.0AspXaa: 0.0 ± 0.0
Glu
3.885GluAla: 3.885 ± 0.177
0.229GluCys: 0.229 ± 0.031
2.908GluAsp: 2.908 ± 0.128
4.633GluGlu: 4.633 ± 0.147
3.801GluPhe: 3.801 ± 0.123
2.258GluGly: 2.258 ± 0.119
0.966GluHis: 0.966 ± 0.074
7.443GluIle: 7.443 ± 0.193
7.693GluLys: 7.693 ± 0.18
7.577GluLeu: 7.577 ± 0.211
1.318GluMet: 1.318 ± 0.086
5.983GluAsn: 5.983 ± 0.17
1.365GluPro: 1.365 ± 0.073
2.861GluGln: 2.861 ± 0.126
2.011GluArg: 2.011 ± 0.084
3.816GluSer: 3.816 ± 0.146
3.271GluThr: 3.271 ± 0.129
3.939GluVal: 3.939 ± 0.122
0.646GluTrp: 0.646 ± 0.056
3.162GluTyr: 3.162 ± 0.113
0.0GluXaa: 0.0 ± 0.0
Phe
3.097PheAla: 3.097 ± 0.137
0.305PheCys: 0.305 ± 0.038
3.743PheAsp: 3.743 ± 0.162
3.83PheGlu: 3.83 ± 0.156
2.687PhePhe: 2.687 ± 0.145
2.52PheGly: 2.52 ± 0.111
0.592PheHis: 0.592 ± 0.055
4.676PheIle: 4.676 ± 0.194
4.771PheLys: 4.771 ± 0.173
5.341PheLeu: 5.341 ± 0.208
0.773PheMet: 0.773 ± 0.062
4.164PheAsn: 4.164 ± 0.137
1.216PhePro: 1.216 ± 0.073
1.311PheGln: 1.311 ± 0.062
1.361PheArg: 1.361 ± 0.081
3.783PheSer: 3.783 ± 0.14
2.483PheThr: 2.483 ± 0.096
3.406PheVal: 3.406 ± 0.143
0.675PheTrp: 0.675 ± 0.051
2.095PheTyr: 2.095 ± 0.106
0.0PheXaa: 0.0 ± 0.0
Gly
2.705GlyAla: 2.705 ± 0.12
0.218GlyCys: 0.218 ± 0.027
2.414GlyAsp: 2.414 ± 0.109
2.531GlyGlu: 2.531 ± 0.114
2.614GlyPhe: 2.614 ± 0.114
2.596GlyGly: 2.596 ± 0.162
0.755GlyHis: 0.755 ± 0.071
4.215GlyIle: 4.215 ± 0.163
3.87GlyLys: 3.87 ± 0.154
3.968GlyLeu: 3.968 ± 0.138
0.922GlyMet: 0.922 ± 0.065
2.229GlyAsn: 2.229 ± 0.103
0.9GlyPro: 0.9 ± 0.059
1.47GlyGln: 1.47 ± 0.077
1.496GlyArg: 1.496 ± 0.106
2.821GlySer: 2.821 ± 0.107
2.814GlyThr: 2.814 ± 0.124
3.115GlyVal: 3.115 ± 0.125
0.476GlyTrp: 0.476 ± 0.05
2.171GlyTyr: 2.171 ± 0.093
0.0GlyXaa: 0.0 ± 0.0
His
0.81HisAla: 0.81 ± 0.056
0.105HisCys: 0.105 ± 0.021
0.766HisAsp: 0.766 ± 0.052
1.078HisGlu: 1.078 ± 0.064
0.922HisPhe: 0.922 ± 0.065
0.77HisGly: 0.77 ± 0.065
0.261HisHis: 0.261 ± 0.036
1.144HisIle: 1.144 ± 0.066
1.162HisLys: 1.162 ± 0.065
1.412HisLeu: 1.412 ± 0.072
0.254HisMet: 0.254 ± 0.028
1.042HisAsn: 1.042 ± 0.076
0.512HisPro: 0.512 ± 0.045
0.479HisGln: 0.479 ± 0.04
0.403HisArg: 0.403 ± 0.046
0.995HisSer: 0.995 ± 0.069
0.643HisThr: 0.643 ± 0.046
0.795HisVal: 0.795 ± 0.062
0.145HisTrp: 0.145 ± 0.026
0.548HisTyr: 0.548 ± 0.044
0.0HisXaa: 0.0 ± 0.0
Ile
5.039IleAla: 5.039 ± 0.158
0.53IleCys: 0.53 ± 0.058
5.787IleAsp: 5.787 ± 0.165
6.121IleGlu: 6.121 ± 0.197
4.734IlePhe: 4.734 ± 0.213
3.994IleGly: 3.994 ± 0.185
1.307IleHis: 1.307 ± 0.074
7.04IleIle: 7.04 ± 0.266
8.278IleLys: 8.278 ± 0.188
8.173IleLeu: 8.173 ± 0.277
1.227IleMet: 1.227 ± 0.079
7.722IleAsn: 7.722 ± 0.242
2.578IlePro: 2.578 ± 0.107
3.293IleGln: 3.293 ± 0.112
2.429IleArg: 2.429 ± 0.102
6.51IleSer: 6.51 ± 0.166
4.604IleThr: 4.604 ± 0.145
5.116IleVal: 5.116 ± 0.169
0.821IleTrp: 0.821 ± 0.059
3.467IleTyr: 3.467 ± 0.115
0.0IleXaa: 0.0 ± 0.0
Lys
5.235LysAla: 5.235 ± 0.288
0.298LysCys: 0.298 ± 0.034
5.453LysAsp: 5.453 ± 0.209
7.639LysGlu: 7.639 ± 0.19
4.143LysPhe: 4.143 ± 0.157
3.42LysGly: 3.42 ± 0.153
1.249LysHis: 1.249 ± 0.075
9.316LysIle: 9.316 ± 0.224
8.321LysLys: 8.321 ± 0.214
8.971LysLeu: 8.971 ± 0.194
2.331LysMet: 2.331 ± 0.103
8.615LysAsn: 8.615 ± 0.203
2.331LysPro: 2.331 ± 0.111
3.58LysGln: 3.58 ± 0.162
2.955LysArg: 2.955 ± 0.105
5.617LysSer: 5.617 ± 0.154
5.46LysThr: 5.46 ± 0.162
5.326LysVal: 5.326 ± 0.152
1.104LysTrp: 1.104 ± 0.069
4.469LysTyr: 4.469 ± 0.142
0.0LysXaa: 0.0 ± 0.0
Leu
5.519LeuAla: 5.519 ± 0.172
0.541LeuCys: 0.541 ± 0.05
6.107LeuAsp: 6.107 ± 0.18
6.713LeuGlu: 6.713 ± 0.164
4.956LeuPhe: 4.956 ± 0.176
4.31LeuGly: 4.31 ± 0.159
1.231LeuHis: 1.231 ± 0.08
9.138LeuIle: 9.138 ± 0.263
9.614LeuLys: 9.614 ± 0.189
7.831LeuLeu: 7.831 ± 0.267
1.652LeuMet: 1.652 ± 0.094
8.205LeuAsn: 8.205 ± 0.306
2.629LeuPro: 2.629 ± 0.098
2.625LeuGln: 2.625 ± 0.104
2.861LeuArg: 2.861 ± 0.11
7.403LeuSer: 7.403 ± 0.156
5.159LeuThr: 5.159 ± 0.191
5.918LeuVal: 5.918 ± 0.168
0.73LeuTrp: 0.73 ± 0.049
2.904LeuTyr: 2.904 ± 0.124
0.0LeuXaa: 0.0 ± 0.0
Met
0.806MetAla: 0.806 ± 0.069
0.116MetCys: 0.116 ± 0.019
0.842MetAsp: 0.842 ± 0.061
0.842MetGlu: 0.842 ± 0.055
0.886MetPhe: 0.886 ± 0.058
0.465MetGly: 0.465 ± 0.046
0.301MetHis: 0.301 ± 0.033
1.561MetIle: 1.561 ± 0.088
1.764MetLys: 1.764 ± 0.084
1.503MetLeu: 1.503 ± 0.09
0.37MetMet: 0.37 ± 0.042
1.507MetAsn: 1.507 ± 0.085
0.519MetPro: 0.519 ± 0.048
0.654MetGln: 0.654 ± 0.04
0.595MetArg: 0.595 ± 0.056
1.361MetSer: 1.361 ± 0.08
0.842MetThr: 0.842 ± 0.063
0.908MetVal: 0.908 ± 0.077
0.12MetTrp: 0.12 ± 0.021
0.548MetTyr: 0.548 ± 0.051
0.0MetXaa: 0.0 ± 0.0
Asn
4.266AsnAla: 4.266 ± 0.238
0.269AsnCys: 0.269 ± 0.034
5.083AsnAsp: 5.083 ± 0.232
5.707AsnGlu: 5.707 ± 0.174
4.179AsnPhe: 4.179 ± 0.148
3.551AsnGly: 3.551 ± 0.121
1.027AsnHis: 1.027 ± 0.065
5.947AsnIle: 5.947 ± 0.168
7.668AsnLys: 7.668 ± 0.185
8.285AsnLeu: 8.285 ± 0.262
1.078AsnMet: 1.078 ± 0.069
6.39AsnAsn: 6.39 ± 0.281
2.694AsnPro: 2.694 ± 0.119
3.536AsnGln: 3.536 ± 0.205
2.135AsnArg: 2.135 ± 0.098
5.845AsnSer: 5.845 ± 0.187
3.74AsnThr: 3.74 ± 0.177
4.135AsnVal: 4.135 ± 0.105
0.9AsnTrp: 0.9 ± 0.065
3.344AsnTyr: 3.344 ± 0.112
0.0AsnXaa: 0.0 ± 0.0
Pro
1.514ProAla: 1.514 ± 0.083
0.102ProCys: 0.102 ± 0.018
1.405ProAsp: 1.405 ± 0.068
2.193ProGlu: 2.193 ± 0.083
1.438ProPhe: 1.438 ± 0.079
1.238ProGly: 1.238 ± 0.072
0.476ProHis: 0.476 ± 0.047
2.396ProIle: 2.396 ± 0.097
2.498ProLys: 2.498 ± 0.103
2.142ProLeu: 2.142 ± 0.102
0.341ProMet: 0.341 ± 0.035
2.298ProAsn: 2.298 ± 0.099
0.436ProPro: 0.436 ± 0.045
1.027ProGln: 1.027 ± 0.062
0.715ProArg: 0.715 ± 0.048
1.783ProSer: 1.783 ± 0.082
1.467ProThr: 1.467 ± 0.079
1.674ProVal: 1.674 ± 0.105
0.251ProTrp: 0.251 ± 0.032
1.133ProTyr: 1.133 ± 0.07
0.0ProXaa: 0.0 ± 0.0
Gln
2.236GlnAla: 2.236 ± 0.169
0.102GlnCys: 0.102 ± 0.018
1.939GlnAsp: 1.939 ± 0.118
2.759GlnGlu: 2.759 ± 0.121
1.627GlnPhe: 1.627 ± 0.082
1.369GlnGly: 1.369 ± 0.065
0.512GlnHis: 0.512 ± 0.05
3.95GlnIle: 3.95 ± 0.154
4.368GlnLys: 4.368 ± 0.145
3.239GlnLeu: 3.239 ± 0.133
0.791GlnMet: 0.791 ± 0.062
3.881GlnAsn: 3.881 ± 0.164
0.94GlnPro: 0.94 ± 0.055
1.285GlnGln: 1.285 ± 0.077
1.205GlnArg: 1.205 ± 0.075
2.138GlnSer: 2.138 ± 0.106
1.906GlnThr: 1.906 ± 0.104
1.87GlnVal: 1.87 ± 0.071
0.356GlnTrp: 0.356 ± 0.034
1.256GlnTyr: 1.256 ± 0.07
0.0GlnXaa: 0.0 ± 0.0
Arg
1.619ArgAla: 1.619 ± 0.082
0.142ArgCys: 0.142 ± 0.023
1.87ArgAsp: 1.87 ± 0.084
2.364ArgGlu: 2.364 ± 0.101
1.463ArgPhe: 1.463 ± 0.074
1.278ArgGly: 1.278 ± 0.079
0.374ArgHis: 0.374 ± 0.039
2.799ArgIle: 2.799 ± 0.107
2.774ArgLys: 2.774 ± 0.122
2.868ArgLeu: 2.868 ± 0.123
0.646ArgMet: 0.646 ± 0.059
2.059ArgAsn: 2.059 ± 0.088
0.748ArgPro: 0.748 ± 0.055
1.053ArgGln: 1.053 ± 0.062
1.173ArgArg: 1.173 ± 0.07
1.783ArgSer: 1.783 ± 0.086
1.434ArgThr: 1.434 ± 0.087
1.783ArgVal: 1.783 ± 0.095
0.247ArgTrp: 0.247 ± 0.034
1.271ArgTyr: 1.271 ± 0.076
0.0ArgXaa: 0.0 ± 0.0
Ser
3.634SerAla: 3.634 ± 0.127
0.323SerCys: 0.323 ± 0.041
4.422SerAsp: 4.422 ± 0.147
4.843SerGlu: 4.843 ± 0.162
3.834SerPhe: 3.834 ± 0.124
3.46SerGly: 3.46 ± 0.143
0.955SerHis: 0.955 ± 0.065
5.657SerIle: 5.657 ± 0.182
6.386SerLys: 6.386 ± 0.15
6.811SerLeu: 6.811 ± 0.188
0.875SerMet: 0.875 ± 0.057
5.028SerAsn: 5.028 ± 0.167
1.452SerPro: 1.452 ± 0.071
2.734SerGln: 2.734 ± 0.108
1.808SerArg: 1.808 ± 0.099
4.763SerSer: 4.763 ± 0.147
3.569SerThr: 3.569 ± 0.161
3.543SerVal: 3.543 ± 0.109
0.744SerTrp: 0.744 ± 0.055
2.723SerTyr: 2.723 ± 0.11
0.0SerXaa: 0.0 ± 0.0
Thr
2.48ThrAla: 2.48 ± 0.144
0.163ThrCys: 0.163 ± 0.028
2.712ThrAsp: 2.712 ± 0.15
2.752ThrGlu: 2.752 ± 0.114
2.839ThrPhe: 2.839 ± 0.108
2.589ThrGly: 2.589 ± 0.117
0.911ThrHis: 0.911 ± 0.069
4.825ThrIle: 4.825 ± 0.154
5.097ThrLys: 5.097 ± 0.177
5.145ThrLeu: 5.145 ± 0.155
0.545ThrMet: 0.545 ± 0.045
4.346ThrAsn: 4.346 ± 0.199
1.968ThrPro: 1.968 ± 0.099
2.276ThrGln: 2.276 ± 0.134
1.652ThrArg: 1.652 ± 0.084
3.493ThrSer: 3.493 ± 0.116
2.93ThrThr: 2.93 ± 0.139
2.727ThrVal: 2.727 ± 0.109
0.537ThrTrp: 0.537 ± 0.041
2.207ThrTyr: 2.207 ± 0.101
0.0ThrXaa: 0.0 ± 0.0
Val
3.721ValAla: 3.721 ± 0.122
0.294ValCys: 0.294 ± 0.038
3.805ValAsp: 3.805 ± 0.128
4.012ValGlu: 4.012 ± 0.14
3.072ValPhe: 3.072 ± 0.124
2.545ValGly: 2.545 ± 0.132
0.886ValHis: 0.886 ± 0.065
4.869ValIle: 4.869 ± 0.159
4.89ValLys: 4.89 ± 0.131
5.203ValLeu: 5.203 ± 0.159
0.882ValMet: 0.882 ± 0.062
4.034ValAsn: 4.034 ± 0.131
1.601ValPro: 1.601 ± 0.103
2.117ValGln: 2.117 ± 0.096
1.739ValArg: 1.739 ± 0.098
4.143ValSer: 4.143 ± 0.143
3.115ValThr: 3.115 ± 0.119
4.143ValVal: 4.143 ± 0.152
0.483ValTrp: 0.483 ± 0.039
2.131ValTyr: 2.131 ± 0.09
0.0ValXaa: 0.0 ± 0.0
Trp
0.53TrpAla: 0.53 ± 0.043
0.062TrpCys: 0.062 ± 0.014
0.574TrpAsp: 0.574 ± 0.049
0.614TrpGlu: 0.614 ± 0.05
0.606TrpPhe: 0.606 ± 0.055
0.425TrpGly: 0.425 ± 0.046
0.102TrpHis: 0.102 ± 0.019
1.013TrpIle: 1.013 ± 0.069
0.831TrpLys: 0.831 ± 0.061
0.904TrpLeu: 0.904 ± 0.072
0.243TrpMet: 0.243 ± 0.034
0.882TrpAsn: 0.882 ± 0.054
0.236TrpPro: 0.236 ± 0.028
0.298TrpGln: 0.298 ± 0.037
0.301TrpArg: 0.301 ± 0.03
0.657TrpSer: 0.657 ± 0.054
0.523TrpThr: 0.523 ± 0.044
0.559TrpVal: 0.559 ± 0.046
0.171TrpTrp: 0.171 ± 0.029
0.432TrpTyr: 0.432 ± 0.039
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.088TyrAla: 2.088 ± 0.099
0.211TyrCys: 0.211 ± 0.027
2.701TyrAsp: 2.701 ± 0.106
2.694TyrGlu: 2.694 ± 0.102
2.491TyrPhe: 2.491 ± 0.126
1.743TyrGly: 1.743 ± 0.098
0.614TyrHis: 0.614 ± 0.06
2.836TyrIle: 2.836 ± 0.098
3.841TyrLys: 3.841 ± 0.125
4.15TyrLeu: 4.15 ± 0.137
0.555TyrMet: 0.555 ± 0.043
2.912TyrAsn: 2.912 ± 0.117
1.104TyrPro: 1.104 ± 0.066
1.594TyrGln: 1.594 ± 0.085
1.347TyrArg: 1.347 ± 0.07
3.18TyrSer: 3.18 ± 0.121
2.12TyrThr: 2.12 ± 0.099
2.295TyrVal: 2.295 ± 0.105
0.494TyrTrp: 0.494 ± 0.047
1.797TyrTyr: 1.797 ± 0.094
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 701 proteins (275436 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski