Beihai picorna-like virus 117

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KHE6|A0A1L3KHE6_9VIRU Uncharacterized protein OS=Beihai picorna-like virus 117 OX=1922546 PE=4 SV=1
MM1 pKa = 8.18ASGQPLATAAPVVDD15 pKa = 4.8SAATPAGPLSLEE27 pKa = 4.36TGRR30 pKa = 11.84TTHH33 pKa = 6.08QPLSTVTGNFNSLDD47 pKa = 3.55PFLYY51 pKa = 9.81RR52 pKa = 11.84HH53 pKa = 5.94NFIIANFRR61 pKa = 11.84WNADD65 pKa = 3.39KK66 pKa = 11.34APGNLIWQLPLSPYY80 pKa = 10.61NLGGRR85 pKa = 11.84IAEE88 pKa = 3.98RR89 pKa = 11.84AAAYY93 pKa = 9.1LYY95 pKa = 10.09WRR97 pKa = 11.84GQISFEE103 pKa = 4.42VIVVATGFHH112 pKa = 6.71AGQLFIVEE120 pKa = 4.7TPPDD124 pKa = 4.09YY125 pKa = 11.11KK126 pKa = 10.66PGSTQSISDD135 pKa = 3.98LSTFHH140 pKa = 7.19HH141 pKa = 6.22EE142 pKa = 4.21QVDD145 pKa = 4.05VKK147 pKa = 11.13VGVPIQFSTRR157 pKa = 11.84DD158 pKa = 3.11ICPTQFHH165 pKa = 5.97TVFDD169 pKa = 4.11SQSTLKK175 pKa = 9.24PTEE178 pKa = 4.42SVTGMQSIGGYY189 pKa = 9.78ISMFNLTKK197 pKa = 10.01LTAANTAPEE206 pKa = 3.49ISIIVKK212 pKa = 10.36ACLDD216 pKa = 3.54PSFEE220 pKa = 4.13VSIMRR225 pKa = 11.84PLSFDD230 pKa = 3.39NEE232 pKa = 4.01RR233 pKa = 11.84DD234 pKa = 3.55EE235 pKa = 5.23VEE237 pKa = 3.91TGRR240 pKa = 11.84WQLLAEE246 pKa = 4.45RR247 pKa = 11.84LNDD250 pKa = 3.38NAKK253 pKa = 10.73DD254 pKa = 3.9YY255 pKa = 11.03GVKK258 pKa = 10.64DD259 pKa = 3.4SDD261 pKa = 3.86VAGLFLVGGSTEE273 pKa = 3.39AWRR276 pKa = 11.84GVDD279 pKa = 3.74RR280 pKa = 11.84LGNAANPYY288 pKa = 8.73TGRR291 pKa = 11.84NMEE294 pKa = 4.66KK295 pKa = 10.69LLMAPCSITGNVNKK309 pKa = 10.29EE310 pKa = 4.11DD311 pKa = 3.94IYY313 pKa = 11.45LSDD316 pKa = 5.15DD317 pKa = 3.59YY318 pKa = 11.69TPITHH323 pKa = 6.71PVGRR327 pKa = 11.84FNMKK331 pKa = 9.93IVDD334 pKa = 3.73PEE336 pKa = 4.02FNAVKK341 pKa = 10.05NQTYY345 pKa = 8.95FYY347 pKa = 8.8PIAYY351 pKa = 6.49GTSGSATYY359 pKa = 9.31TKK361 pKa = 9.74YY362 pKa = 9.54TNTDD366 pKa = 2.6WYY368 pKa = 10.07LGRR371 pKa = 11.84VSSDD375 pKa = 2.48KK376 pKa = 11.34YY377 pKa = 10.0YY378 pKa = 11.16VCTNPTRR385 pKa = 11.84TSFHH389 pKa = 6.35KK390 pKa = 10.85VITPSGYY397 pKa = 9.78CLHH400 pKa = 6.57VKK402 pKa = 10.2PSDD405 pKa = 3.18ITGPSVTALNGEE417 pKa = 3.99NLLYY421 pKa = 9.85TRR423 pKa = 11.84CGTGSVAGTLTEE435 pKa = 4.32SDD437 pKa = 3.68SLCFKK442 pKa = 10.6EE443 pKa = 3.96ITEE446 pKa = 4.25FCIQHH451 pKa = 7.0KK452 pKa = 8.77ITLTSNTPVFRR463 pKa = 11.84ILDD466 pKa = 3.72RR467 pKa = 11.84VTKK470 pKa = 10.27QVLGHH475 pKa = 6.59ARR477 pKa = 11.84LNKK480 pKa = 10.01QGFLSTNFPSEE491 pKa = 3.99TVVFYY496 pKa = 10.9EE497 pKa = 4.18DD498 pKa = 2.98VMLAFEE504 pKa = 4.56GMYY507 pKa = 10.3SEE509 pKa = 6.34LEE511 pKa = 4.1DD512 pKa = 3.98LPEE515 pKa = 5.37ISVTASTYY523 pKa = 10.92LLQKK527 pKa = 11.03AMTRR531 pKa = 11.84SFEE534 pKa = 4.46RR535 pKa = 11.84MVNKK539 pKa = 10.46NKK541 pKa = 9.65TKK543 pKa = 10.25SKK545 pKa = 10.85NGISSSSYY553 pKa = 8.16GSSRR557 pKa = 11.84RR558 pKa = 11.84FRR560 pKa = 11.84GRR562 pKa = 11.84RR563 pKa = 11.84APVVYY568 pKa = 10.07HH569 pKa = 5.77RR570 pKa = 11.84QSYY573 pKa = 10.93DD574 pKa = 3.02GGKK577 pKa = 9.91EE578 pKa = 3.65EE579 pKa = 4.01PTYY582 pKa = 11.04DD583 pKa = 3.47RR584 pKa = 11.84EE585 pKa = 4.4SSQEE589 pKa = 3.75RR590 pKa = 11.84YY591 pKa = 8.51EE592 pKa = 4.32SKK594 pKa = 10.16RR595 pKa = 11.84SQRR598 pKa = 11.84RR599 pKa = 11.84FKK601 pKa = 10.95RR602 pKa = 11.84KK603 pKa = 8.05TNTLHH608 pKa = 6.67SKK610 pKa = 10.4RR611 pKa = 11.84SEE613 pKa = 4.06ATDD616 pKa = 4.0DD617 pKa = 4.2GSDD620 pKa = 3.6SEE622 pKa = 5.22FEE624 pKa = 3.64ILEE627 pKa = 4.13YY628 pKa = 10.3PEE630 pKa = 4.67CSNPEE635 pKa = 3.67RR636 pKa = 11.84TEE638 pKa = 4.17CPEE641 pKa = 4.22CPAPGWPHH649 pKa = 7.01ADD651 pKa = 3.14VDD653 pKa = 4.3PEE655 pKa = 4.39SGEE658 pKa = 3.95LTDD661 pKa = 4.99VSNEE665 pKa = 3.82PTGEE669 pKa = 4.04RR670 pKa = 11.84RR671 pKa = 11.84DD672 pKa = 3.76SSIDD676 pKa = 3.32ALLEE680 pKa = 3.96RR681 pKa = 11.84LTQLGARR688 pKa = 11.84ATRR691 pKa = 11.84VFGRR695 pKa = 11.84RR696 pKa = 11.84NLQDD700 pKa = 3.07VV701 pKa = 3.66

Molecular weight: 78.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KHQ4|A0A1L3KHQ4_9VIRU Uncharacterized protein OS=Beihai picorna-like virus 117 OX=1922546 PE=4 SV=1
MM1 pKa = 7.52ASAAALMGAAGVSAGGGLLSSIIDD25 pKa = 3.82NPMMEE30 pKa = 4.19AKK32 pKa = 10.28KK33 pKa = 10.56NRR35 pKa = 11.84LTIEE39 pKa = 4.06NQAKK43 pKa = 10.7NDD45 pKa = 3.78MKK47 pKa = 11.37VNAANEE53 pKa = 4.14GLSEE57 pKa = 4.24KK58 pKa = 9.32LTHH61 pKa = 5.35YY62 pKa = 7.63TANEE66 pKa = 3.83AKK68 pKa = 10.26QLMTAQTANSKK79 pKa = 10.36SLNTQNAQIQKK90 pKa = 9.74EE91 pKa = 4.23LNAQSAQLQAGLMQTSTQNQEE112 pKa = 3.68NLQTFQMNQLEE123 pKa = 4.35KK124 pKa = 11.02DD125 pKa = 4.02GIPRR129 pKa = 11.84SMLYY133 pKa = 10.66LSGSRR138 pKa = 11.84NSVPEE143 pKa = 3.45QRR145 pKa = 11.84AYY147 pKa = 11.13LGGGTFKK154 pKa = 10.56TFNGLSGNTAYY165 pKa = 9.75TGTQAQQMLNMGNPLAFRR183 pKa = 11.84YY184 pKa = 9.4QSRR187 pKa = 11.84NSNFGTRR194 pKa = 11.84TNTASLQKK202 pKa = 10.82GFDD205 pKa = 3.4KK206 pKa = 10.89ASQYY210 pKa = 10.59IGQFKK215 pKa = 9.78PNKK218 pKa = 9.81SKK220 pKa = 11.05GNQVAAYY227 pKa = 8.34SAKK230 pKa = 10.2YY231 pKa = 9.67DD232 pKa = 3.92DD233 pKa = 4.66VMLVPP238 pKa = 5.46

Molecular weight: 25.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

3218

238

2279

1072.7

120.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.433 ± 0.971

1.336 ± 0.231

5.221 ± 0.486

5.5 ± 0.365

4.071 ± 0.2

5.718 ± 0.796

2.859 ± 0.673

5.904 ± 1.021

6.37 ± 0.774

7.955 ± 0.202

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.268 ± 0.48

5.252 ± 0.865

5.065 ± 0.449

4.817 ± 0.934

5.003 ± 0.479

7.427 ± 0.749

8.359 ± 0.227

6.091 ± 0.652

0.622 ± 0.143

3.729 ± 0.141
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski