Streptomyces sp. ST5x

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5564 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S9PQW3|A0A2S9PQW3_9ACTN ABC transporter ATP-binding protein OS=Streptomyces sp. ST5x OX=2100817 GN=C6N75_23650 PE=4 SV=1
MM1 pKa = 7.5KK2 pKa = 8.87PTVTEE7 pKa = 5.42ALAEE11 pKa = 4.19WKK13 pKa = 10.22DD14 pKa = 3.42AWDD17 pKa = 4.09EE18 pKa = 4.2LQSSAVNALCLALPGLDD35 pKa = 3.18HH36 pKa = 7.08TKK38 pKa = 10.01TPTYY42 pKa = 9.72CCPVMLNISKK52 pKa = 10.48PNDD55 pKa = 3.53LGDD58 pKa = 3.72GRR60 pKa = 11.84VCVDD64 pKa = 3.56DD65 pKa = 3.81DD66 pKa = 3.66TRR68 pKa = 11.84ATVEE72 pKa = 4.33LNDD75 pKa = 3.69VPNEE79 pKa = 4.16VIAEE83 pKa = 4.07AVDD86 pKa = 3.65AVFGIAWFDD95 pKa = 3.5QAEE98 pKa = 4.73GPLEE102 pKa = 4.21DD103 pKa = 5.15AGPGTYY109 pKa = 10.24NYY111 pKa = 10.7DD112 pKa = 3.56DD113 pKa = 4.02EE114 pKa = 4.73QTGGEE119 pKa = 4.32YY120 pKa = 10.56EE121 pKa = 4.49VVLGDD126 pKa = 3.38NGTNTGRR133 pKa = 11.84VYY135 pKa = 10.78VGYY138 pKa = 10.96VPVPYY143 pKa = 10.23AAEE146 pKa = 4.1LLDD149 pKa = 5.03AISTARR155 pKa = 11.84EE156 pKa = 3.8RR157 pKa = 11.84QVQRR161 pKa = 11.84ATAGDD166 pKa = 3.5

Molecular weight: 17.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S9PQK4|A0A2S9PQK4_9ACTN NADH-quinone oxidoreductase subunit K OS=Streptomyces sp. ST5x OX=2100817 GN=nuoK PE=3 SV=1
MM1 pKa = 7.31SGSRR5 pKa = 11.84STPTGAGGRR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84VRR18 pKa = 11.84RR19 pKa = 11.84GLTGTAAAVTAMAALTASQAPGLPAFAAAPAPAQTAAAVGGGPLRR64 pKa = 11.84TAGRR68 pKa = 11.84TAAGLPAAGPPAHH81 pKa = 7.01RR82 pKa = 11.84RR83 pKa = 11.84APVPPAPPAPPRR95 pKa = 11.84ARR97 pKa = 11.84PRR99 pKa = 11.84PTLL102 pKa = 3.53

Molecular weight: 9.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5564

0

5564

1765270

24

5184

317.3

33.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.714 ± 0.064

0.772 ± 0.008

5.971 ± 0.033

5.689 ± 0.037

2.613 ± 0.018

9.796 ± 0.032

2.232 ± 0.015

2.794 ± 0.026

2.052 ± 0.031

10.266 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.667 ± 0.014

1.599 ± 0.018

6.29 ± 0.031

2.631 ± 0.021

8.327 ± 0.04

4.719 ± 0.029

5.885 ± 0.027

8.481 ± 0.03

1.466 ± 0.015

2.036 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski