Amino acid dipepetide frequency for Streptomyces sp. ST5x

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	26.011AlaAla: 26.011 ± 0.269	1.036AlaCys: 1.036 ± 0.025	9.189AlaAsp: 9.189 ± 0.069	9.538AlaGlu: 9.538 ± 0.1	3.641AlaPhe: 3.641 ± 0.052	15.057AlaGly: 15.057 ± 0.113	2.917AlaHis: 2.917 ± 0.048	3.18AlaIle: 3.18 ± 0.052	2.921AlaLys: 2.921 ± 0.067	15.383AlaLeu: 15.383 ± 0.154	2.52AlaMet: 2.52 ± 0.045	1.863AlaAsn: 1.863 ± 0.039	8.096AlaPro: 8.096 ± 0.088	3.659AlaGln: 3.659 ± 0.051	11.362AlaArg: 11.362 ± 0.1	5.775AlaSer: 5.775 ± 0.063	6.895AlaThr: 6.895 ± 0.071	13.225AlaVal: 13.225 ± 0.11	1.893AlaTrp: 1.893 ± 0.036	2.974AlaTyr: 2.974 ± 0.048	0.001AlaXaa: 0.001 ± 0.001
Cys	1.19CysAla: 1.19 ± 0.025	0.092CysCys: 0.092 ± 0.007	0.439CysAsp: 0.439 ± 0.014	0.364CysGlu: 0.364 ± 0.014	0.192CysPhe: 0.192 ± 0.011	0.973CysGly: 0.973 ± 0.026	0.184CysHis: 0.184 ± 0.012	0.129CysIle: 0.129 ± 0.009	0.112CysLys: 0.112 ± 0.008	0.736CysLeu: 0.736 ± 0.022	0.112CysMet: 0.112 ± 0.007	0.13CysAsn: 0.13 ± 0.008	0.496CysPro: 0.496 ± 0.019	0.137CysGln: 0.137 ± 0.008	0.628CysArg: 0.628 ± 0.019	0.38CysSer: 0.38 ± 0.017	0.493CysThr: 0.493 ± 0.017	0.681CysVal: 0.681 ± 0.017	0.127CysTrp: 0.127 ± 0.009	0.133CysTyr: 0.133 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	8.449AspAla: 8.449 ± 0.086	0.437AspCys: 0.437 ± 0.015	3.432AspAsp: 3.432 ± 0.056	3.787AspGlu: 3.787 ± 0.054	1.601AspPhe: 1.601 ± 0.033	6.715AspGly: 6.715 ± 0.068	1.375AspHis: 1.375 ± 0.029	1.578AspIle: 1.578 ± 0.029	1.177AspLys: 1.177 ± 0.033	6.045AspLeu: 6.045 ± 0.072	0.752AspMet: 0.752 ± 0.022	0.864AspAsn: 0.864 ± 0.024	4.65AspPro: 4.65 ± 0.056	1.541AspGln: 1.541 ± 0.03	5.107AspArg: 5.107 ± 0.066	2.264AspSer: 2.264 ± 0.037	3.175AspThr: 3.175 ± 0.043	4.634AspVal: 4.634 ± 0.06	1.049AspTrp: 1.049 ± 0.027	1.078AspTyr: 1.078 ± 0.024	0.0AspXaa: 0.0 ± 0.0
Glu	7.674GluAla: 7.674 ± 0.092	0.348GluCys: 0.348 ± 0.014	3.017GluAsp: 3.017 ± 0.049	3.636GluGlu: 3.636 ± 0.064	1.32GluPhe: 1.32 ± 0.031	4.368GluGly: 4.368 ± 0.058	1.502GluHis: 1.502 ± 0.036	1.989GluIle: 1.989 ± 0.037	1.437GluLys: 1.437 ± 0.041	6.802GluLeu: 6.802 ± 0.091	0.845GluMet: 0.845 ± 0.022	0.899GluAsn: 0.899 ± 0.023	3.5GluPro: 3.5 ± 0.05	2.219GluGln: 2.219 ± 0.038	5.765GluArg: 5.765 ± 0.065	2.391GluSer: 2.391 ± 0.036	2.812GluThr: 2.812 ± 0.049	4.596GluVal: 4.596 ± 0.055	0.723GluTrp: 0.723 ± 0.02	1.043GluTyr: 1.043 ± 0.024	0.0GluXaa: 0.0 ± 0.0
Phe	3.806PheAla: 3.806 ± 0.047	0.263PheCys: 0.263 ± 0.013	1.891PheAsp: 1.891 ± 0.037	1.344PheGlu: 1.344 ± 0.031	0.838PhePhe: 0.838 ± 0.025	2.855PheGly: 2.855 ± 0.043	0.589PheHis: 0.589 ± 0.018	0.592PheIle: 0.592 ± 0.02	0.458PheLys: 0.458 ± 0.018	2.487PheLeu: 2.487 ± 0.044	0.364PheMet: 0.364 ± 0.016	0.521PheAsn: 0.521 ± 0.018	1.362PhePro: 1.362 ± 0.027	0.642PheGln: 0.642 ± 0.017	1.729PheArg: 1.729 ± 0.033	1.353PheSer: 1.353 ± 0.027	2.055PheThr: 2.055 ± 0.037	2.035PheVal: 2.035 ± 0.044	0.411PheTrp: 0.411 ± 0.016	0.534PheTyr: 0.534 ± 0.018	0.0PheXaa: 0.0 ± 0.0
Gly	12.566GlyAla: 12.566 ± 0.107	0.868GlyCys: 0.868 ± 0.024	5.433GlyAsp: 5.433 ± 0.057	5.391GlyGlu: 5.391 ± 0.055	2.768GlyPhe: 2.768 ± 0.049	10.134GlyGly: 10.134 ± 0.116	2.27GlyHis: 2.27 ± 0.033	3.024GlyIle: 3.024 ± 0.043	2.304GlyLys: 2.304 ± 0.058	9.583GlyLeu: 9.583 ± 0.097	1.995GlyMet: 1.995 ± 0.033	1.621GlyAsn: 1.621 ± 0.038	5.831GlyPro: 5.831 ± 0.069	2.724GlyGln: 2.724 ± 0.049	8.668GlyArg: 8.668 ± 0.074	5.281GlySer: 5.281 ± 0.061	5.719GlyThr: 5.719 ± 0.065	7.728GlyVal: 7.728 ± 0.083	1.685GlyTrp: 1.685 ± 0.032	2.362GlyTyr: 2.362 ± 0.045	0.001GlyXaa: 0.001 ± 0.001
His	2.617HisAla: 2.617 ± 0.037	0.203HisCys: 0.203 ± 0.01	1.253HisAsp: 1.253 ± 0.028	1.093HisGlu: 1.093 ± 0.027	0.619HisPhe: 0.619 ± 0.019	2.477HisGly: 2.477 ± 0.035	0.695HisHis: 0.695 ± 0.023	0.592HisIle: 0.592 ± 0.018	0.313HisLys: 0.313 ± 0.014	2.408HisLeu: 2.408 ± 0.043	0.293HisMet: 0.293 ± 0.014	0.33HisAsn: 0.33 ± 0.015	1.856HisPro: 1.856 ± 0.03	0.66HisGln: 0.66 ± 0.016	2.225HisArg: 2.225 ± 0.043	0.905HisSer: 0.905 ± 0.024	1.332HisThr: 1.332 ± 0.026	1.611HisVal: 1.611 ± 0.028	0.378HisTrp: 0.378 ± 0.016	0.457HisTyr: 0.457 ± 0.018	0.0HisXaa: 0.0 ± 0.0
Ile	4.479IleAla: 4.479 ± 0.052	0.249IleCys: 0.249 ± 0.011	1.945IleAsp: 1.945 ± 0.041	1.76IleGlu: 1.76 ± 0.039	0.58IlePhe: 0.58 ± 0.02	3.213IleGly: 3.213 ± 0.048	0.503IleHis: 0.503 ± 0.018	0.726IleIle: 0.726 ± 0.026	0.617IleLys: 0.617 ± 0.023	2.116IleLeu: 2.116 ± 0.04	0.39IleMet: 0.39 ± 0.015	0.591IleAsn: 0.591 ± 0.02	1.544IlePro: 1.544 ± 0.033	0.642IleGln: 0.642 ± 0.021	2.052IleArg: 2.052 ± 0.042	1.384IleSer: 1.384 ± 0.028	1.979IleThr: 1.979 ± 0.041	2.414IleVal: 2.414 ± 0.04	0.316IleTrp: 0.316 ± 0.016	0.442IleTyr: 0.442 ± 0.014	0.0IleXaa: 0.0 ± 0.0
Lys	2.89LysAla: 2.89 ± 0.054	0.109LysCys: 0.109 ± 0.008	1.269LysAsp: 1.269 ± 0.037	1.121LysGlu: 1.121 ± 0.032	0.41LysPhe: 0.41 ± 0.015	1.716LysGly: 1.716 ± 0.041	0.389LysHis: 0.389 ± 0.015	0.796LysIle: 0.796 ± 0.027	0.786LysLys: 0.786 ± 0.033	2.17LysLeu: 2.17 ± 0.046	0.374LysMet: 0.374 ± 0.016	0.486LysAsn: 0.486 ± 0.02	1.276LysPro: 1.276 ± 0.035	0.634LysGln: 0.634 ± 0.024	1.404LysArg: 1.404 ± 0.033	1.032LysSer: 1.032 ± 0.028	1.25LysThr: 1.25 ± 0.034	1.801LysVal: 1.801 ± 0.041	0.211LysTrp: 0.211 ± 0.011	0.389LysTyr: 0.389 ± 0.017	0.0LysXaa: 0.0 ± 0.0
Leu	16.027LeuAla: 16.027 ± 0.153	0.845LeuCys: 0.845 ± 0.022	6.732LeuAsp: 6.732 ± 0.08	4.634LeuGlu: 4.634 ± 0.06	2.498LeuPhe: 2.498 ± 0.039	9.229LeuGly: 9.229 ± 0.081	2.259LeuHis: 2.259 ± 0.037	2.862LeuIle: 2.862 ± 0.049	1.986LeuLys: 1.986 ± 0.041	11.367LeuLeu: 11.367 ± 0.127	1.549LeuMet: 1.549 ± 0.029	1.508LeuAsn: 1.508 ± 0.032	6.672LeuPro: 6.672 ± 0.076	1.974LeuGln: 1.974 ± 0.036	8.684LeuArg: 8.684 ± 0.081	4.937LeuSer: 4.937 ± 0.058	6.817LeuThr: 6.817 ± 0.068	9.047LeuVal: 9.047 ± 0.087	1.221LeuTrp: 1.221 ± 0.03	1.81LeuTyr: 1.81 ± 0.034	0.0LeuXaa: 0.0 ± 0.0
Met	2.308MetAla: 2.308 ± 0.038	0.138MetCys: 0.138 ± 0.009	0.917MetAsp: 0.917 ± 0.025	0.749MetGlu: 0.749 ± 0.023	0.434MetPhe: 0.434 ± 0.017	1.284MetGly: 1.284 ± 0.03	0.317MetHis: 0.317 ± 0.012	0.592MetIle: 0.592 ± 0.02	0.371MetLys: 0.371 ± 0.015	1.592MetLeu: 1.592 ± 0.032	0.261MetMet: 0.261 ± 0.013	0.39MetAsn: 0.39 ± 0.016	1.055MetPro: 1.055 ± 0.023	0.42MetGln: 0.42 ± 0.016	1.363MetArg: 1.363 ± 0.03	1.225MetSer: 1.225 ± 0.027	1.45MetThr: 1.45 ± 0.028	1.276MetVal: 1.276 ± 0.027	0.21MetTrp: 0.21 ± 0.01	0.317MetTyr: 0.317 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	2.106AsnAla: 2.106 ± 0.038	0.141AsnCys: 0.141 ± 0.008	0.867AsnAsp: 0.867 ± 0.025	0.737AsnGlu: 0.737 ± 0.023	0.44AsnPhe: 0.44 ± 0.017	1.708AsnGly: 1.708 ± 0.037	0.352AsnHis: 0.352 ± 0.013	0.594AsnIle: 0.594 ± 0.017	0.383AsnLys: 0.383 ± 0.016	1.509AsnLeu: 1.509 ± 0.031	0.28AsnMet: 0.28 ± 0.013	0.369AsnAsn: 0.369 ± 0.016	1.2AsnPro: 1.2 ± 0.027	0.484AsnGln: 0.484 ± 0.017	1.15AsnArg: 1.15 ± 0.026	0.766AsnSer: 0.766 ± 0.022	1.046AsnThr: 1.046 ± 0.028	1.268AsnVal: 1.268 ± 0.03	0.241AsnTrp: 0.241 ± 0.012	0.352AsnTyr: 0.352 ± 0.015	0.0AsnXaa: 0.0 ± 0.0
Pro	10.399ProAla: 10.399 ± 0.113	0.325ProCys: 0.325 ± 0.014	4.531ProAsp: 4.531 ± 0.054	4.407ProGlu: 4.407 ± 0.055	1.505ProPhe: 1.505 ± 0.029	7.283ProGly: 7.283 ± 0.077	1.409ProHis: 1.409 ± 0.027	1.096ProIle: 1.096 ± 0.026	1.119ProLys: 1.119 ± 0.028	5.532ProLeu: 5.532 ± 0.066	0.944ProMet: 0.944 ± 0.024	0.781ProAsn: 0.781 ± 0.024	3.893ProPro: 3.893 ± 0.064	1.675ProGln: 1.675 ± 0.04	4.172ProArg: 4.172 ± 0.053	3.001ProSer: 3.001 ± 0.05	2.818ProThr: 2.818 ± 0.037	5.689ProVal: 5.689 ± 0.068	0.899ProTrp: 0.899 ± 0.025	1.425ProTyr: 1.425 ± 0.029	0.001ProXaa: 0.001 ± 0.001
Gln	3.737GlnAla: 3.737 ± 0.046	0.169GlnCys: 0.169 ± 0.011	1.377GlnAsp: 1.377 ± 0.031	1.364GlnGlu: 1.364 ± 0.035	0.62GlnPhe: 0.62 ± 0.02	2.315GlnGly: 2.315 ± 0.048	0.617GlnHis: 0.617 ± 0.02	0.965GlnIle: 0.965 ± 0.026	0.555GlnLys: 0.555 ± 0.021	2.627GlnLeu: 2.627 ± 0.038	0.426GlnMet: 0.426 ± 0.019	0.432GlnAsn: 0.432 ± 0.017	1.729GlnPro: 1.729 ± 0.035	1.181GlnGln: 1.181 ± 0.039	2.334GlnArg: 2.334 ± 0.041	1.209GlnSer: 1.209 ± 0.027	1.317GlnThr: 1.317 ± 0.034	2.355GlnVal: 2.355 ± 0.037	0.43GlnTrp: 0.43 ± 0.017	0.553GlnTyr: 0.553 ± 0.018	0.0GlnXaa: 0.0 ± 0.0
Arg	10.837ArgAla: 10.837 ± 0.099	0.604ArgCys: 0.604 ± 0.019	4.461ArgAsp: 4.461 ± 0.055	4.921ArgGlu: 4.921 ± 0.063	2.298ArgPhe: 2.298 ± 0.037	6.286ArgGly: 6.286 ± 0.061	2.159ArgHis: 2.159 ± 0.038	3.085ArgIle: 3.085 ± 0.043	1.639ArgLys: 1.639 ± 0.043	8.82ArgLeu: 8.82 ± 0.081	1.813ArgMet: 1.813 ± 0.032	1.271ArgAsn: 1.271 ± 0.028	5.524ArgPro: 5.524 ± 0.065	2.272ArgGln: 2.272 ± 0.034	8.47ArgArg: 8.47 ± 0.095	3.946ArgSer: 3.946 ± 0.045	5.675ArgThr: 5.675 ± 0.065	5.992ArgVal: 5.992 ± 0.058	1.41ArgTrp: 1.41 ± 0.027	1.786ArgTyr: 1.786 ± 0.03	0.0ArgXaa: 0.0 ± 0.0
Ser	6.743SerAla: 6.743 ± 0.071	0.375SerCys: 0.375 ± 0.016	2.412SerAsp: 2.412 ± 0.037	2.193SerGlu: 2.193 ± 0.037	1.444SerPhe: 1.444 ± 0.028	5.698SerGly: 5.698 ± 0.065	0.966SerHis: 0.966 ± 0.022	1.241SerIle: 1.241 ± 0.032	0.908SerLys: 0.908 ± 0.027	4.474SerLeu: 4.474 ± 0.051	0.969SerMet: 0.969 ± 0.024	0.756SerAsn: 0.756 ± 0.023	3.052SerPro: 3.052 ± 0.039	1.097SerGln: 1.097 ± 0.025	3.484SerArg: 3.484 ± 0.051	2.576SerSer: 2.576 ± 0.048	2.8SerThr: 2.8 ± 0.06	4.004SerVal: 4.004 ± 0.055	0.827SerTrp: 0.827 ± 0.022	1.175SerTyr: 1.175 ± 0.03	0.001SerXaa: 0.001 ± 0.001
Thr	9.574ThrAla: 9.574 ± 0.106	0.398ThrCys: 0.398 ± 0.017	3.343ThrAsp: 3.343 ± 0.044	3.13ThrGlu: 3.13 ± 0.043	1.409ThrPhe: 1.409 ± 0.033	6.908ThrGly: 6.908 ± 0.073	1.071ThrHis: 1.071 ± 0.025	1.484ThrIle: 1.484 ± 0.033	1.009ThrLys: 1.009 ± 0.03	5.53ThrLeu: 5.53 ± 0.064	0.896ThrMet: 0.896 ± 0.021	0.833ThrAsn: 0.833 ± 0.024	3.956ThrPro: 3.956 ± 0.046	1.112ThrGln: 1.112 ± 0.029	3.756ThrArg: 3.756 ± 0.047	2.866ThrSer: 2.866 ± 0.051	3.607ThrThr: 3.607 ± 0.068	5.964ThrVal: 5.964 ± 0.064	0.832ThrTrp: 0.832 ± 0.022	1.17ThrTyr: 1.17 ± 0.029	0.0ThrXaa: 0.0 ± 0.0
Val	11.028ValAla: 11.028 ± 0.092	0.804ValCys: 0.804 ± 0.02	4.94ValAsp: 4.94 ± 0.047	4.765ValGlu: 4.765 ± 0.057	2.419ValPhe: 2.419 ± 0.036	6.433ValGly: 6.433 ± 0.067	2.021ValHis: 2.021 ± 0.036	2.541ValIle: 2.541 ± 0.04	1.711ValLys: 1.711 ± 0.036	9.745ValLeu: 9.745 ± 0.097	1.365ValMet: 1.365 ± 0.028	1.621ValAsn: 1.621 ± 0.029	5.361ValPro: 5.361 ± 0.063	2.029ValGln: 2.029 ± 0.035	7.708ValArg: 7.708 ± 0.073	4.186ValSer: 4.186 ± 0.053	5.397ValThr: 5.397 ± 0.059	7.956ValVal: 7.956 ± 0.086	1.155ValTrp: 1.155 ± 0.027	1.622ValTyr: 1.622 ± 0.029	0.001ValXaa: 0.001 ± 0.001
Trp	1.677TrpAla: 1.677 ± 0.033	0.142TrpCys: 0.142 ± 0.008	0.819TrpAsp: 0.819 ± 0.021	0.702TrpGlu: 0.702 ± 0.02	0.48TrpPhe: 0.48 ± 0.016	1.033TrpGly: 1.033 ± 0.025	0.361TrpHis: 0.361 ± 0.014	0.461TrpIle: 0.461 ± 0.016	0.344TrpLys: 0.344 ± 0.017	1.684TrpLeu: 1.684 ± 0.04	0.291TrpMet: 0.291 ± 0.014	0.384TrpAsn: 0.384 ± 0.019	0.796TrpPro: 0.796 ± 0.024	0.583TrpGln: 0.583 ± 0.018	1.361TrpArg: 1.361 ± 0.03	0.892TrpSer: 0.892 ± 0.028	1.017TrpThr: 1.017 ± 0.025	0.963TrpVal: 0.963 ± 0.024	0.326TrpTrp: 0.326 ± 0.015	0.348TrpTyr: 0.348 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.02TyrAla: 3.02 ± 0.038	0.181TyrCys: 0.181 ± 0.01	1.443TyrAsp: 1.443 ± 0.033	1.249TyrGlu: 1.249 ± 0.026	0.611TyrPhe: 0.611 ± 0.019	2.27TyrGly: 2.27 ± 0.044	0.363TyrHis: 0.363 ± 0.015	0.416TyrIle: 0.416 ± 0.014	0.365TyrLys: 0.365 ± 0.017	2.05TyrLeu: 2.05 ± 0.036	0.23TyrMet: 0.23 ± 0.012	0.341TyrAsn: 0.341 ± 0.014	1.054TyrPro: 1.054 ± 0.025	0.627TyrGln: 0.627 ± 0.02	1.849TyrArg: 1.849 ± 0.035	0.823TyrSer: 0.823 ± 0.024	1.194TyrThr: 1.194 ± 0.027	1.568TyrVal: 1.568 ± 0.032	0.319TyrTrp: 0.319 ± 0.013	0.387TyrTyr: 0.387 ± 0.015	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 5564 proteins (1765270 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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