Streptococcus phage Javan273

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A7F1|A0A4D6A7F1_9CAUD Tail protein OS=Streptococcus phage Javan273 OX=2548088 GN=Javan273_0014 PE=4 SV=1
MM1 pKa = 6.92ATKK4 pKa = 10.54EE5 pKa = 3.9EE6 pKa = 4.35VIAFAKK12 pKa = 10.5NLADD16 pKa = 3.48TGQGVDD22 pKa = 4.87LDD24 pKa = 4.72GVWGTQCVDD33 pKa = 3.53LPNWITTKK41 pKa = 10.41YY42 pKa = 10.5FSVALWGNAIDD53 pKa = 5.36LLDD56 pKa = 3.79SAKK59 pKa = 10.31AQGMEE64 pKa = 4.17VVYY67 pKa = 10.06DD68 pKa = 3.81APGVNPRR75 pKa = 11.84DD76 pKa = 3.23GAIFVMVTYY85 pKa = 10.75AHH87 pKa = 7.21GYY89 pKa = 7.86GHH91 pKa = 7.22TGLVIQTSDD100 pKa = 3.4GYY102 pKa = 11.11VLYY105 pKa = 10.01TIEE108 pKa = 4.29QNVDD112 pKa = 3.0GYY114 pKa = 11.54SDD116 pKa = 3.84NNGDD120 pKa = 4.5GINDD124 pKa = 3.41QLQFGGPARR133 pKa = 11.84YY134 pKa = 9.36INRR137 pKa = 11.84PFSDD141 pKa = 3.17GTGYY145 pKa = 10.47ILGWFYY151 pKa = 10.79PPYY154 pKa = 10.67NNTPAQQLEE163 pKa = 4.23TSAPVKK169 pKa = 10.64SEE171 pKa = 3.8SDD173 pKa = 3.16GTYY176 pKa = 9.8VANPEE181 pKa = 4.2TGTFTVRR188 pKa = 11.84VAALNVRR195 pKa = 11.84SAPRR199 pKa = 11.84LDD201 pKa = 3.6AEE203 pKa = 4.28IVATYY208 pKa = 9.87GEE210 pKa = 4.27NMEE213 pKa = 4.47FNYY216 pKa = 10.8DD217 pKa = 3.03GWLDD221 pKa = 3.37SDD223 pKa = 4.15GYY225 pKa = 10.74IWVTYY230 pKa = 10.42ISATGIRR237 pKa = 11.84RR238 pKa = 11.84YY239 pKa = 10.09VAVGNSKK246 pKa = 10.33NGRR249 pKa = 11.84RR250 pKa = 11.84VTNFGTFRR258 pKa = 4.37

Molecular weight: 28.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AB52|A0A4D6AB52_9CAUD Terminase small subunit OS=Streptococcus phage Javan273 OX=2548088 GN=Javan273_0026 PE=4 SV=1
MM1 pKa = 7.84RR2 pKa = 11.84GLDD5 pKa = 3.68EE6 pKa = 5.22FIKK9 pKa = 10.62HH10 pKa = 6.16AMKK13 pKa = 10.43QKK15 pKa = 10.51QEE17 pKa = 3.98VKK19 pKa = 10.25SAVDD23 pKa = 3.71FEE25 pKa = 4.66IGQAALRR32 pKa = 11.84VEE34 pKa = 4.33RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.09ILAPVDD44 pKa = 3.77TGWLRR49 pKa = 11.84TQIYY53 pKa = 10.01NEE55 pKa = 4.27KK56 pKa = 9.58QRR58 pKa = 11.84MMHH61 pKa = 5.91YY62 pKa = 10.3KK63 pKa = 10.15VISPALYY70 pKa = 9.93SVYY73 pKa = 10.79LEE75 pKa = 4.19LGTRR79 pKa = 11.84YY80 pKa = 9.16MSAQPYY86 pKa = 10.18LDD88 pKa = 3.74PALRR92 pKa = 11.84AEE94 pKa = 4.57WPVLMANLKK103 pKa = 10.68KK104 pKa = 10.07MFRR107 pKa = 11.84RR108 pKa = 3.88

Molecular weight: 12.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

11584

37

1364

236.4

26.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.674 ± 0.728

0.423 ± 0.11

6.259 ± 0.498

6.967 ± 0.591

3.626 ± 0.172

6.215 ± 0.459

1.148 ± 0.163

7.096 ± 0.341

8.106 ± 0.526

7.907 ± 0.299

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.616 ± 0.274

6.06 ± 0.335

2.823 ± 0.258

4.273 ± 0.197

4.204 ± 0.342

6.854 ± 0.85

6.673 ± 0.357

6.259 ± 0.249

1.183 ± 0.177

3.634 ± 0.385
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski