Thermobaculum terrenum (strain ATCC BAA-798 / YNP1)
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2827 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D1CGV8|D1CGV8_THET1 Oligopeptide/dipeptide ABC transporter ATPase subunit OS=Thermobaculum terrenum (strain ATCC BAA-798 / YNP1) OX=525904 GN=Tter_2076 PE=4 SV=1
MM1 pKa = 6.98 TRR3 pKa = 11.84 KK4 pKa = 8.58 LTGRR8 pKa = 11.84 LATLLMLLAALSMAALATGNSVPPSGLSTSTSSIGPNALKK48 pKa = 10.41 PSQCASLNLTNLLVVNGFGFSPHH71 pKa = 6.13 VPTLILGSSGPDD83 pKa = 3.25 YY84 pKa = 10.96 IFAGIDD90 pKa = 3.38 DD91 pKa = 4.36 DD92 pKa = 5.98 CIVAGAGNDD101 pKa = 4.09 FISGSDD107 pKa = 3.42 GDD109 pKa = 5.13 DD110 pKa = 3.66 IILAGSGADD119 pKa = 3.64 YY120 pKa = 11.51 VNGGPGYY127 pKa = 9.65 DD128 pKa = 2.69 ICYY131 pKa = 10.42 GVFDD135 pKa = 5.25 PGDD138 pKa = 3.44 TAVGCEE144 pKa = 3.82 VRR146 pKa = 11.84 LPP148 pKa = 3.65
Molecular weight: 14.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.776
IPC2_protein 3.872
IPC_protein 3.846
Toseland 3.617
ProMoST 4.062
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.897
Rodwell 3.681
Grimsley 3.528
Solomon 3.859
Lehninger 3.821
Nozaki 4.012
DTASelect 4.342
Thurlkill 3.706
EMBOSS 3.897
Sillero 3.986
Patrickios 0.54
IPC_peptide 3.859
IPC2_peptide 3.948
IPC2.peptide.svr19 3.849
Protein with the highest isoelectric point:
>tr|D1CCJ8|D1CCJ8_THET1 Cell division protein FtsW OS=Thermobaculum terrenum (strain ATCC BAA-798 / YNP1) OX=525904 GN=Tter_1607 PE=4 SV=1
MM1 pKa = 7.38 RR2 pKa = 11.84 QANGEE7 pKa = 4.03 GTVYY11 pKa = 10.44 RR12 pKa = 11.84 RR13 pKa = 11.84 RR14 pKa = 11.84 DD15 pKa = 3.84 GRR17 pKa = 11.84 WVAAVVHH24 pKa = 6.4 AGRR27 pKa = 11.84 RR28 pKa = 11.84 ISRR31 pKa = 11.84 YY32 pKa = 9.09 AKK34 pKa = 9.2 TRR36 pKa = 11.84 RR37 pKa = 11.84 EE38 pKa = 3.91 AQKK41 pKa = 10.09 QLRR44 pKa = 11.84 EE45 pKa = 4.08 LLEE48 pKa = 4.1 AAAEE52 pKa = 4.15 HH53 pKa = 6.88 RR54 pKa = 11.84 LVPPNKK60 pKa = 9.21 LTLEE64 pKa = 4.08 QYY66 pKa = 9.61 LAEE69 pKa = 4.01 WLEE72 pKa = 3.91 VRR74 pKa = 11.84 SRR76 pKa = 11.84 EE77 pKa = 4.08 LRR79 pKa = 11.84 VSTRR83 pKa = 11.84 VNYY86 pKa = 9.67 QRR88 pKa = 11.84 LIEE91 pKa = 4.0 QHH93 pKa = 5.95 ICPALGGRR101 pKa = 11.84 RR102 pKa = 11.84 LQALAALEE110 pKa = 4.0 LSRR113 pKa = 11.84 WLAGLGEE120 pKa = 4.3 RR121 pKa = 11.84 LPRR124 pKa = 11.84 RR125 pKa = 11.84 AQEE128 pKa = 4.3 AYY130 pKa = 10.54 ALLHH134 pKa = 6.46 KK135 pKa = 10.66 ALADD139 pKa = 3.68 AVRR142 pKa = 11.84 LGLLATNPLDD152 pKa = 3.61 RR153 pKa = 11.84 VEE155 pKa = 4.39 PPKK158 pKa = 10.21 HH159 pKa = 4.65 QRR161 pKa = 11.84 RR162 pKa = 11.84 RR163 pKa = 11.84 PTLPARR169 pKa = 11.84 DD170 pKa = 3.85 QIALLIAALEE180 pKa = 4.18 AGKK183 pKa = 10.49 AGWYY187 pKa = 10.02 SEE189 pKa = 4.82 LLLFLLGSGCRR200 pKa = 11.84 IGEE203 pKa = 4.02 ALGLEE208 pKa = 4.24 WGDD211 pKa = 3.62 VDD213 pKa = 3.51 WQEE216 pKa = 3.78 GSVRR220 pKa = 11.84 ISRR223 pKa = 11.84 QLLEE227 pKa = 4.29 VGGEE231 pKa = 4.18 VHH233 pKa = 7.04 EE234 pKa = 4.91 SPPKK238 pKa = 9.38 SRR240 pKa = 11.84 AGEE243 pKa = 3.92 RR244 pKa = 11.84 VIALPAFALEE254 pKa = 4.13 ALRR257 pKa = 11.84 QQRR260 pKa = 11.84 AKK262 pKa = 10.93 KK263 pKa = 9.55 LGKK266 pKa = 10.2 YY267 pKa = 9.77 CFLSSTGTTPCRR279 pKa = 11.84 RR280 pKa = 11.84 NVLRR284 pKa = 11.84 ALHH287 pKa = 6.19 AVCAEE292 pKa = 4.06 LGLPRR297 pKa = 11.84 LRR299 pKa = 11.84 IHH301 pKa = 7.28 DD302 pKa = 4.27 LRR304 pKa = 11.84 HH305 pKa = 4.33 VHH307 pKa = 6.99 ASLLAHH313 pKa = 6.89 AGVPPKK319 pKa = 10.35 VAQSRR324 pKa = 11.84 LGHH327 pKa = 6.01 SSPMVTLQVYY337 pKa = 7.19 QHH339 pKa = 6.17 VLDD342 pKa = 4.44 GADD345 pKa = 3.36 RR346 pKa = 11.84 EE347 pKa = 4.3 AALRR351 pKa = 11.84 LEE353 pKa = 4.41 RR354 pKa = 11.84 VLGG357 pKa = 3.87
Molecular weight: 39.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.349
IPC2_protein 9.531
IPC_protein 10.496
Toseland 10.555
ProMoST 10.35
Dawson 10.672
Bjellqvist 10.438
Wikipedia 10.921
Rodwell 10.745
Grimsley 10.73
Solomon 10.818
Lehninger 10.774
Nozaki 10.57
DTASelect 10.423
Thurlkill 10.584
EMBOSS 10.979
Sillero 10.613
Patrickios 10.409
IPC_peptide 10.818
IPC2_peptide 9.604
IPC2.peptide.svr19 8.618
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2827
0
2827
935529
31
2240
330.9
36.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.905 ± 0.044
0.891 ± 0.015
5.212 ± 0.034
6.492 ± 0.054
3.321 ± 0.029
7.857 ± 0.041
2.099 ± 0.02
6.107 ± 0.044
3.622 ± 0.038
10.798 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.291 ± 0.017
2.927 ± 0.028
5.202 ± 0.035
3.484 ± 0.028
6.83 ± 0.045
6.343 ± 0.049
4.963 ± 0.028
7.824 ± 0.04
1.608 ± 0.022
3.223 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here