Sphingomonas sp. Leaf343

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; unclassified Sphingomonas

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3242 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q5Q2M9|A0A0Q5Q2M9_9SPHN Uncharacterized protein OS=Sphingomonas sp. Leaf343 OX=1736345 GN=ASG07_04355 PE=3 SV=1
MM1 pKa = 7.42TYY3 pKa = 10.71VVTDD7 pKa = 3.09ACIRR11 pKa = 11.84CKK13 pKa = 11.04YY14 pKa = 7.9MDD16 pKa = 4.38CVEE19 pKa = 4.32VCPVDD24 pKa = 3.79CFYY27 pKa = 11.19EE28 pKa = 4.58GEE30 pKa = 4.18NMLVINPSEE39 pKa = 4.67CIDD42 pKa = 4.07CGVCEE47 pKa = 4.44PEE49 pKa = 4.82CPAEE53 pKa = 5.13AILPDD58 pKa = 3.88TEE60 pKa = 4.22NGQEE64 pKa = 4.04KK65 pKa = 9.39WLEE68 pKa = 3.95LNATFSAQWPNVTRR82 pKa = 11.84KK83 pKa = 8.85MDD85 pKa = 3.7QTPPDD90 pKa = 4.06ADD92 pKa = 3.27EE93 pKa = 4.26MKK95 pKa = 10.66GVEE98 pKa = 4.04GKK100 pKa = 10.1YY101 pKa = 10.79DD102 pKa = 3.35KK103 pKa = 10.91FFSPEE108 pKa = 4.16PGTGDD113 pKa = 3.05

Molecular weight: 12.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q5Q041|A0A0Q5Q041_9SPHN 2-keto-4-pentenoate hydratase OS=Sphingomonas sp. Leaf343 OX=1736345 GN=ASG07_01000 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.58GFRR19 pKa = 11.84SRR21 pKa = 11.84MATVGGRR28 pKa = 11.84SVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3242

0

3242

1059892

29

3392

326.9

35.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.953 ± 0.075

0.674 ± 0.011

6.231 ± 0.034

4.807 ± 0.044

3.418 ± 0.027

9.235 ± 0.048

1.864 ± 0.02

4.781 ± 0.029

2.568 ± 0.03

9.645 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.327 ± 0.023

2.392 ± 0.031

5.389 ± 0.034

2.948 ± 0.022

7.72 ± 0.05

4.873 ± 0.032

5.851 ± 0.048

7.672 ± 0.034

1.443 ± 0.021

2.211 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski