Amino acid dipepetide frequency for Sphingomonas sp. Leaf343

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.865AlaAla: 21.865 ± 0.26
1.005AlaCys: 1.005 ± 0.035
8.27AlaAsp: 8.27 ± 0.1
7.152AlaGlu: 7.152 ± 0.093
4.368AlaPhe: 4.368 ± 0.075
12.917AlaGly: 12.917 ± 0.145
2.161AlaHis: 2.161 ± 0.048
7.09AlaIle: 7.09 ± 0.092
3.833AlaLys: 3.833 ± 0.075
13.828AlaLeu: 13.828 ± 0.165
3.972AlaMet: 3.972 ± 0.068
3.188AlaAsn: 3.188 ± 0.057
6.592AlaPro: 6.592 ± 0.101
4.311AlaGln: 4.311 ± 0.07
10.383AlaArg: 10.383 ± 0.139
6.429AlaSer: 6.429 ± 0.08
8.291AlaThr: 8.291 ± 0.106
9.614AlaVal: 9.614 ± 0.098
1.712AlaTrp: 1.712 ± 0.045
2.548AlaTyr: 2.548 ± 0.047
0.002AlaXaa: 0.002 ± 0.001
Cys
0.893CysAla: 0.893 ± 0.031
0.075CysCys: 0.075 ± 0.009
0.485CysAsp: 0.485 ± 0.022
0.317CysGlu: 0.317 ± 0.019
0.261CysPhe: 0.261 ± 0.017
0.76CysGly: 0.76 ± 0.03
0.167CysHis: 0.167 ± 0.012
0.326CysIle: 0.326 ± 0.02
0.134CysLys: 0.134 ± 0.012
0.552CysLeu: 0.552 ± 0.024
0.127CysMet: 0.127 ± 0.011
0.162CysAsn: 0.162 ± 0.014
0.376CysPro: 0.376 ± 0.022
0.149CysGln: 0.149 ± 0.012
0.499CysArg: 0.499 ± 0.025
0.371CysSer: 0.371 ± 0.02
0.351CysThr: 0.351 ± 0.018
0.489CysVal: 0.489 ± 0.025
0.087CysTrp: 0.087 ± 0.01
0.155CysTyr: 0.155 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
8.904AspAla: 8.904 ± 0.104
0.396AspCys: 0.396 ± 0.02
3.643AspAsp: 3.643 ± 0.075
3.108AspGlu: 3.108 ± 0.064
2.064AspPhe: 2.064 ± 0.04
6.135AspGly: 6.135 ± 0.101
1.337AspHis: 1.337 ± 0.037
2.75AspIle: 2.75 ± 0.057
1.394AspLys: 1.394 ± 0.038
5.899AspLeu: 5.899 ± 0.081
1.332AspMet: 1.332 ± 0.038
1.254AspAsn: 1.254 ± 0.036
3.96AspPro: 3.96 ± 0.076
1.773AspGln: 1.773 ± 0.038
5.695AspArg: 5.695 ± 0.078
2.079AspSer: 2.079 ± 0.048
3.224AspThr: 3.224 ± 0.059
4.6AspVal: 4.6 ± 0.065
1.152AspTrp: 1.152 ± 0.031
1.611AspTyr: 1.611 ± 0.046
0.001AspXaa: 0.001 ± 0.001
Glu
7.048GluAla: 7.048 ± 0.087
0.254GluCys: 0.254 ± 0.017
2.484GluAsp: 2.484 ± 0.054
2.279GluGlu: 2.279 ± 0.059
1.304GluPhe: 1.304 ± 0.04
4.063GluGly: 4.063 ± 0.066
1.057GluHis: 1.057 ± 0.034
2.296GluIle: 2.296 ± 0.049
1.454GluLys: 1.454 ± 0.043
4.332GluLeu: 4.332 ± 0.071
1.199GluMet: 1.199 ± 0.035
1.127GluAsn: 1.127 ± 0.037
2.53GluPro: 2.53 ± 0.061
1.817GluGln: 1.817 ± 0.042
4.863GluArg: 4.863 ± 0.079
1.859GluSer: 1.859 ± 0.045
2.9GluThr: 2.9 ± 0.056
3.517GluVal: 3.517 ± 0.064
0.763GluTrp: 0.763 ± 0.025
0.923GluTyr: 0.923 ± 0.03
0.0GluXaa: 0.0 ± 0.0
Phe
5.029PheAla: 5.029 ± 0.08
0.271PheCys: 0.271 ± 0.018
2.673PheAsp: 2.673 ± 0.055
1.756PheGlu: 1.756 ± 0.044
1.221PhePhe: 1.221 ± 0.041
3.652PheGly: 3.652 ± 0.059
0.669PheHis: 0.669 ± 0.025
1.153PheIle: 1.153 ± 0.034
0.693PheLys: 0.693 ± 0.027
2.877PheLeu: 2.877 ± 0.066
0.668PheMet: 0.668 ± 0.026
0.885PheAsn: 0.885 ± 0.035
1.391PhePro: 1.391 ± 0.037
0.878PheGln: 0.878 ± 0.029
2.345PheArg: 2.345 ± 0.045
1.745PheSer: 1.745 ± 0.044
2.073PheThr: 2.073 ± 0.047
2.847PheVal: 2.847 ± 0.057
0.489PheTrp: 0.489 ± 0.021
0.86PheTyr: 0.86 ± 0.031
0.0PheXaa: 0.0 ± 0.0
Gly
10.425GlyAla: 10.425 ± 0.098
0.776GlyCys: 0.776 ± 0.03
5.534GlyAsp: 5.534 ± 0.096
4.694GlyGlu: 4.694 ± 0.064
3.698GlyPhe: 3.698 ± 0.064
9.035GlyGly: 9.035 ± 0.156
1.9GlyHis: 1.9 ± 0.045
4.512GlyIle: 4.512 ± 0.063
2.816GlyLys: 2.816 ± 0.062
8.587GlyLeu: 8.587 ± 0.105
2.365GlyMet: 2.365 ± 0.055
2.377GlyAsn: 2.377 ± 0.071
3.807GlyPro: 3.807 ± 0.054
3.098GlyGln: 3.098 ± 0.062
7.291GlyArg: 7.291 ± 0.09
4.888GlySer: 4.888 ± 0.088
5.48GlyThr: 5.48 ± 0.107
6.873GlyVal: 6.873 ± 0.098
1.76GlyTrp: 1.76 ± 0.047
2.433GlyTyr: 2.433 ± 0.052
0.0GlyXaa: 0.0 ± 0.0
His
2.473HisAla: 2.473 ± 0.058
0.178HisCys: 0.178 ± 0.014
1.235HisAsp: 1.235 ± 0.029
0.908HisGlu: 0.908 ± 0.033
0.654HisPhe: 0.654 ± 0.024
1.913HisGly: 1.913 ± 0.043
0.533HisHis: 0.533 ± 0.027
0.755HisIle: 0.755 ± 0.027
0.327HisLys: 0.327 ± 0.017
1.797HisLeu: 1.797 ± 0.04
0.383HisMet: 0.383 ± 0.022
0.392HisAsn: 0.392 ± 0.02
1.242HisPro: 1.242 ± 0.035
0.491HisGln: 0.491 ± 0.022
1.568HisArg: 1.568 ± 0.05
0.76HisSer: 0.76 ± 0.026
0.713HisThr: 0.713 ± 0.027
1.488HisVal: 1.488 ± 0.038
0.353HisTrp: 0.353 ± 0.018
0.476HisTyr: 0.476 ± 0.02
0.0HisXaa: 0.0 ± 0.0
Ile
8.12IleAla: 8.12 ± 0.093
0.348IleCys: 0.348 ± 0.02
4.035IleAsp: 4.035 ± 0.059
2.917IleGlu: 2.917 ± 0.056
1.286IlePhe: 1.286 ± 0.036
5.351IleGly: 5.351 ± 0.087
0.799IleHis: 0.799 ± 0.026
1.511IleIle: 1.511 ± 0.04
0.933IleLys: 0.933 ± 0.035
3.737IleLeu: 3.737 ± 0.07
0.793IleMet: 0.793 ± 0.025
1.086IleAsn: 1.086 ± 0.036
2.108IlePro: 2.108 ± 0.044
1.032IleGln: 1.032 ± 0.037
3.325IleArg: 3.325 ± 0.057
1.995IleSer: 1.995 ± 0.048
2.364IleThr: 2.364 ± 0.048
4.679IleVal: 4.679 ± 0.073
0.532IleTrp: 0.532 ± 0.023
0.859IleTyr: 0.859 ± 0.03
0.0IleXaa: 0.0 ± 0.0
Lys
3.5LysAla: 3.5 ± 0.064
0.106LysCys: 0.106 ± 0.009
1.356LysAsp: 1.356 ± 0.039
0.914LysGlu: 0.914 ± 0.03
0.7LysPhe: 0.7 ± 0.029
2.245LysGly: 2.245 ± 0.048
0.448LysHis: 0.448 ± 0.022
1.156LysIle: 1.156 ± 0.034
0.86LysLys: 0.86 ± 0.04
2.79LysLeu: 2.79 ± 0.053
0.644LysMet: 0.644 ± 0.028
0.593LysAsn: 0.593 ± 0.022
1.851LysPro: 1.851 ± 0.05
0.796LysGln: 0.796 ± 0.028
2.013LysArg: 2.013 ± 0.045
1.327LysSer: 1.327 ± 0.039
1.639LysThr: 1.639 ± 0.042
1.89LysVal: 1.89 ± 0.05
0.3LysTrp: 0.3 ± 0.016
0.549LysTyr: 0.549 ± 0.023
0.0LysXaa: 0.0 ± 0.0
Leu
14.369LeuAla: 14.369 ± 0.168
0.641LeuCys: 0.641 ± 0.024
6.401LeuAsp: 6.401 ± 0.091
4.098LeuGlu: 4.098 ± 0.074
3.465LeuPhe: 3.465 ± 0.073
8.145LeuGly: 8.145 ± 0.099
1.681LeuHis: 1.681 ± 0.043
4.594LeuIle: 4.594 ± 0.071
2.715LeuLys: 2.715 ± 0.054
8.963LeuLeu: 8.963 ± 0.143
2.042LeuMet: 2.042 ± 0.041
2.289LeuAsn: 2.289 ± 0.049
5.563LeuPro: 5.563 ± 0.07
2.278LeuGln: 2.278 ± 0.051
6.729LeuArg: 6.729 ± 0.087
5.697LeuSer: 5.697 ± 0.08
6.066LeuThr: 6.066 ± 0.093
7.466LeuVal: 7.466 ± 0.101
1.246LeuTrp: 1.246 ± 0.041
1.998LeuTyr: 1.998 ± 0.041
0.001LeuXaa: 0.001 ± 0.001
Met
3.131MetAla: 3.131 ± 0.055
0.118MetCys: 0.118 ± 0.009
1.037MetAsp: 1.037 ± 0.038
0.868MetGlu: 0.868 ± 0.029
0.647MetPhe: 0.647 ± 0.025
1.778MetGly: 1.778 ± 0.045
0.378MetHis: 0.378 ± 0.019
1.425MetIle: 1.425 ± 0.038
0.861MetLys: 0.861 ± 0.03
2.675MetLeu: 2.675 ± 0.054
0.641MetMet: 0.641 ± 0.027
0.611MetAsn: 0.611 ± 0.025
1.456MetPro: 1.456 ± 0.038
0.693MetGln: 0.693 ± 0.026
1.754MetArg: 1.754 ± 0.043
1.241MetSer: 1.241 ± 0.035
1.906MetThr: 1.906 ± 0.044
1.531MetVal: 1.531 ± 0.04
0.228MetTrp: 0.228 ± 0.015
0.288MetTyr: 0.288 ± 0.016
0.0MetXaa: 0.0 ± 0.0
Asn
3.117AsnAla: 3.117 ± 0.058
0.189AsnCys: 0.189 ± 0.014
1.44AsnAsp: 1.44 ± 0.044
0.948AsnGlu: 0.948 ± 0.029
0.86AsnPhe: 0.86 ± 0.037
2.427AsnGly: 2.427 ± 0.068
0.455AsnHis: 0.455 ± 0.02
1.182AsnIle: 1.182 ± 0.043
0.522AsnLys: 0.522 ± 0.024
2.28AsnLeu: 2.28 ± 0.055
0.47AsnMet: 0.47 ± 0.024
0.653AsnAsn: 0.653 ± 0.033
1.598AsnPro: 1.598 ± 0.041
0.693AsnGln: 0.693 ± 0.025
1.795AsnArg: 1.795 ± 0.042
1.123AsnSer: 1.123 ± 0.04
1.22AsnThr: 1.22 ± 0.04
1.897AsnVal: 1.897 ± 0.047
0.382AsnTrp: 0.382 ± 0.021
0.67AsnTyr: 0.67 ± 0.026
0.0AsnXaa: 0.0 ± 0.0
Pro
7.779ProAla: 7.779 ± 0.116
0.286ProCys: 0.286 ± 0.017
4.02ProAsp: 4.02 ± 0.07
3.014ProGlu: 3.014 ± 0.056
1.89ProPhe: 1.89 ± 0.044
5.046ProGly: 5.046 ± 0.076
0.993ProHis: 0.993 ± 0.035
2.429ProIle: 2.429 ± 0.05
1.38ProLys: 1.38 ± 0.034
4.966ProLeu: 4.966 ± 0.072
1.166ProMet: 1.166 ± 0.031
1.221ProAsn: 1.221 ± 0.036
2.931ProPro: 2.931 ± 0.082
1.589ProGln: 1.589 ± 0.045
3.199ProArg: 3.199 ± 0.059
2.527ProSer: 2.527 ± 0.051
2.967ProThr: 2.967 ± 0.06
4.711ProVal: 4.711 ± 0.076
0.685ProTrp: 0.685 ± 0.026
1.086ProTyr: 1.086 ± 0.033
0.0ProXaa: 0.0 ± 0.0
Gln
4.093GlnAla: 4.093 ± 0.079
0.167GlnCys: 0.167 ± 0.015
1.387GlnAsp: 1.387 ± 0.04
1.056GlnGlu: 1.056 ± 0.035
1.002GlnPhe: 1.002 ± 0.032
2.48GlnGly: 2.48 ± 0.053
0.556GlnHis: 0.556 ± 0.024
1.462GlnIle: 1.462 ± 0.034
0.739GlnLys: 0.739 ± 0.028
2.848GlnLeu: 2.848 ± 0.057
0.718GlnMet: 0.718 ± 0.027
0.685GlnAsn: 0.685 ± 0.027
1.876GlnPro: 1.876 ± 0.038
1.135GlnGln: 1.135 ± 0.037
2.615GlnArg: 2.615 ± 0.057
1.562GlnSer: 1.562 ± 0.039
1.745GlnThr: 1.745 ± 0.042
2.362GlnVal: 2.362 ± 0.05
0.381GlnTrp: 0.381 ± 0.019
0.614GlnTyr: 0.614 ± 0.026
0.0GlnXaa: 0.0 ± 0.0
Arg
9.373ArgAla: 9.373 ± 0.134
0.465ArgCys: 0.465 ± 0.022
4.825ArgAsp: 4.825 ± 0.082
3.679ArgGlu: 3.679 ± 0.065
3.235ArgPhe: 3.235 ± 0.056
5.656ArgGly: 5.656 ± 0.082
1.69ArgHis: 1.69 ± 0.046
4.237ArgIle: 4.237 ± 0.067
1.722ArgLys: 1.722 ± 0.041
8.422ArgLeu: 8.422 ± 0.108
2.014ArgMet: 2.014 ± 0.047
1.87ArgAsn: 1.87 ± 0.047
4.085ArgPro: 4.085 ± 0.065
2.528ArgGln: 2.528 ± 0.048
6.45ArgArg: 6.45 ± 0.09
3.814ArgSer: 3.814 ± 0.065
4.163ArgThr: 4.163 ± 0.055
5.533ArgVal: 5.533 ± 0.07
1.383ArgTrp: 1.383 ± 0.042
2.053ArgTyr: 2.053 ± 0.048
0.001ArgXaa: 0.001 ± 0.001
Ser
6.127SerAla: 6.127 ± 0.094
0.326SerCys: 0.326 ± 0.019
2.921SerAsp: 2.921 ± 0.054
2.007SerGlu: 2.007 ± 0.046
2.006SerPhe: 2.006 ± 0.049
5.247SerGly: 5.247 ± 0.088
0.825SerHis: 0.825 ± 0.032
2.578SerIle: 2.578 ± 0.051
1.165SerLys: 1.165 ± 0.037
4.681SerLeu: 4.681 ± 0.066
1.051SerMet: 1.051 ± 0.039
1.252SerAsn: 1.252 ± 0.04
2.754SerPro: 2.754 ± 0.053
1.304SerGln: 1.304 ± 0.038
3.442SerArg: 3.442 ± 0.064
2.527SerSer: 2.527 ± 0.061
2.77SerThr: 2.77 ± 0.059
3.726SerVal: 3.726 ± 0.063
0.723SerTrp: 0.723 ± 0.029
1.302SerTyr: 1.302 ± 0.037
0.0SerXaa: 0.0 ± 0.0
Thr
7.347ThrAla: 7.347 ± 0.115
0.351ThrCys: 0.351 ± 0.018
3.282ThrAsp: 3.282 ± 0.061
2.288ThrGlu: 2.288 ± 0.051
1.893ThrPhe: 1.893 ± 0.042
6.135ThrGly: 6.135 ± 0.102
0.895ThrHis: 0.895 ± 0.033
3.386ThrIle: 3.386 ± 0.067
1.293ThrLys: 1.293 ± 0.033
6.603ThrLeu: 6.603 ± 0.087
1.314ThrMet: 1.314 ± 0.04
1.339ThrAsn: 1.339 ± 0.043
3.83ThrPro: 3.83 ± 0.066
1.558ThrGln: 1.558 ± 0.04
4.078ThrArg: 4.078 ± 0.053
2.78ThrSer: 2.78 ± 0.058
3.376ThrThr: 3.376 ± 0.071
4.828ThrVal: 4.828 ± 0.081
0.662ThrTrp: 0.662 ± 0.026
1.274ThrTyr: 1.274 ± 0.043
0.0ThrXaa: 0.0 ± 0.0
Val
11.568ValAla: 11.568 ± 0.105
0.478ValCys: 0.478 ± 0.021
4.913ValAsp: 4.913 ± 0.067
4.509ValGlu: 4.509 ± 0.077
2.269ValPhe: 2.269 ± 0.046
6.206ValGly: 6.206 ± 0.079
1.322ValHis: 1.322 ± 0.042
3.466ValIle: 3.466 ± 0.07
1.896ValLys: 1.896 ± 0.048
6.895ValLeu: 6.895 ± 0.088
1.641ValMet: 1.641 ± 0.043
1.926ValAsn: 1.926 ± 0.048
4.167ValPro: 4.167 ± 0.062
2.116ValGln: 2.116 ± 0.05
5.635ValArg: 5.635 ± 0.066
3.966ValSer: 3.966 ± 0.056
5.182ValThr: 5.182 ± 0.069
6.23ValVal: 6.23 ± 0.09
0.903ValTrp: 0.903 ± 0.029
1.435ValTyr: 1.435 ± 0.035
0.001ValXaa: 0.001 ± 0.001
Trp
1.467TrpAla: 1.467 ± 0.044
0.117TrpCys: 0.117 ± 0.011
0.744TrpAsp: 0.744 ± 0.027
0.534TrpGlu: 0.534 ± 0.025
0.569TrpPhe: 0.569 ± 0.022
1.015TrpGly: 1.015 ± 0.032
0.331TrpHis: 0.331 ± 0.02
0.67TrpIle: 0.67 ± 0.026
0.417TrpLys: 0.417 ± 0.019
1.696TrpLeu: 1.696 ± 0.047
0.308TrpMet: 0.308 ± 0.019
0.422TrpAsn: 0.422 ± 0.02
0.733TrpPro: 0.733 ± 0.03
0.573TrpGln: 0.573 ± 0.022
1.478TrpArg: 1.478 ± 0.044
0.937TrpSer: 0.937 ± 0.029
0.968TrpThr: 0.968 ± 0.032
0.809TrpVal: 0.809 ± 0.028
0.315TrpTrp: 0.315 ± 0.02
0.324TrpTyr: 0.324 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.902TyrAla: 2.902 ± 0.057
0.192TyrCys: 0.192 ± 0.014
1.624TyrAsp: 1.624 ± 0.041
1.023TyrGlu: 1.023 ± 0.034
0.783TyrPhe: 0.783 ± 0.03
2.143TyrGly: 2.143 ± 0.06
0.442TyrHis: 0.442 ± 0.02
0.833TyrIle: 0.833 ± 0.03
0.522TyrLys: 0.522 ± 0.024
2.019TyrLeu: 2.019 ± 0.044
0.417TyrMet: 0.417 ± 0.023
0.592TyrAsn: 0.592 ± 0.029
1.035TyrPro: 1.035 ± 0.032
0.671TyrGln: 0.671 ± 0.028
2.04TyrArg: 2.04 ± 0.049
1.106TyrSer: 1.106 ± 0.037
1.11TyrThr: 1.11 ± 0.042
1.636TyrVal: 1.636 ± 0.047
0.37TyrTrp: 0.37 ± 0.022
0.647TyrTyr: 0.647 ± 0.029
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.002XaaAla: 0.002 ± 0.001
0.0XaaCys: 0.0 ± 0.0
0.001XaaAsp: 0.001 ± 0.001
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.001XaaMet: 0.001 ± 0.001
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.002XaaThr: 0.002 ± 0.002
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3242 proteins (1059892 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski