Sulfitobacter sp. HI0023
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3900 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A166QYQ0|A0A166QYQ0_9RHOB Diaminopimelate decarboxylase OS=Sulfitobacter sp. HI0023 OX=1822225 GN=lysA PE=3 SV=1
MM1 pKa = 7.6 VYY3 pKa = 10.65 VSVQIDD9 pKa = 3.32 THH11 pKa = 7.11 GEE13 pKa = 3.45 SDD15 pKa = 3.0 AAAFEE20 pKa = 4.23 KK21 pKa = 10.81 AIIDD25 pKa = 3.55 LDD27 pKa = 4.42 EE28 pKa = 4.51 IVTCHH33 pKa = 5.41 SVGGGSDD40 pKa = 4.63 FILLVACRR48 pKa = 11.84 DD49 pKa = 3.09 LDD51 pKa = 3.89 AYY53 pKa = 11.05 VSVVRR58 pKa = 11.84 DD59 pKa = 4.13 FGTDD63 pKa = 3.02 CGLSS67 pKa = 3.33
Molecular weight: 7.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.754
IPC2_protein 3.923
IPC_protein 3.859
Toseland 3.643
ProMoST 4.05
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.846
Rodwell 3.694
Grimsley 3.554
Solomon 3.846
Lehninger 3.808
Nozaki 3.999
DTASelect 4.266
Thurlkill 3.719
EMBOSS 3.859
Sillero 3.986
Patrickios 0.782
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.862
Protein with the highest isoelectric point:
>tr|A0A166TZ75|A0A166TZ75_9RHOB sn-glycerol-3-phosphate import ATP-binding protein UgpC OS=Sulfitobacter sp. HI0023 OX=1822225 GN=ugpC PE=3 SV=1
MM1 pKa = 7.25 ATADD5 pKa = 3.91 HH6 pKa = 7.33 PIAPLRR12 pKa = 11.84 HH13 pKa = 5.32 RR14 pKa = 11.84 TLKK17 pKa = 9.83 QRR19 pKa = 11.84 LRR21 pKa = 11.84 RR22 pKa = 11.84 FLAWVRR28 pKa = 11.84 KK29 pKa = 7.13 TVPEE33 pKa = 4.17 WLRR36 pKa = 11.84 LPLGILLMLLGLLGFLPILGFWMIPVGFAVAAMGMRR72 pKa = 11.84 PIRR75 pKa = 11.84 ARR77 pKa = 11.84 WNKK80 pKa = 9.05 RR81 pKa = 11.84 RR82 pKa = 11.84 RR83 pKa = 11.84 GG84 pKa = 3.51
Molecular weight: 9.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.358
IPC2_protein 10.76
IPC_protein 12.369
Toseland 12.53
ProMoST 13.027
Dawson 12.53
Bjellqvist 12.53
Wikipedia 12.998
Rodwell 12.135
Grimsley 12.574
Solomon 13.027
Lehninger 12.925
Nozaki 12.53
DTASelect 12.53
Thurlkill 12.53
EMBOSS 13.027
Sillero 12.53
Patrickios 11.871
IPC_peptide 13.027
IPC2_peptide 12.018
IPC2.peptide.svr19 9.141
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3900
0
3900
1190183
33
1938
305.2
33.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.235 ± 0.058
0.876 ± 0.011
6.147 ± 0.034
6.211 ± 0.038
3.669 ± 0.025
8.735 ± 0.039
2.054 ± 0.018
5.083 ± 0.031
3.133 ± 0.03
9.945 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.821 ± 0.019
2.55 ± 0.021
5.087 ± 0.027
3.157 ± 0.022
7.022 ± 0.039
5.052 ± 0.024
5.407 ± 0.022
7.2 ± 0.031
1.362 ± 0.016
2.254 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here