Streptomyces alni

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7062 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I2ITN9|A0A1I2ITN9_9ACTN Protein N-acetyltransferase RimJ/RimL family OS=Streptomyces alni OX=380248 GN=SAMN05216251_114165 PE=4 SV=1
MM1 pKa = 7.14STPPSEE7 pKa = 5.03AVTPDD12 pKa = 3.4ASSSDD17 pKa = 3.8PAQDD21 pKa = 3.97AGTTQDD27 pKa = 3.07TGTAQDD33 pKa = 3.92AGTALLAFEE42 pKa = 5.23ASDD45 pKa = 3.88VPPLNFVVPEE55 pKa = 4.26SFHH58 pKa = 6.29GLPIAATPEE67 pKa = 3.6EE68 pKa = 4.24RR69 pKa = 11.84AALAEE74 pKa = 4.14TFVRR78 pKa = 11.84DD79 pKa = 4.33LYY81 pKa = 10.82PEE83 pKa = 4.71GSDD86 pKa = 5.58DD87 pKa = 4.01LWTPAAPYY95 pKa = 10.56YY96 pKa = 11.47AMLGEE101 pKa = 4.26AMGDD105 pKa = 3.12QGLAYY110 pKa = 10.37SAMGLFSIDD119 pKa = 3.24EE120 pKa = 5.07GIAHH124 pKa = 7.24CSFTVAAIASDD135 pKa = 3.78HH136 pKa = 6.82PSPEE140 pKa = 3.81VAALGIRR147 pKa = 11.84EE148 pKa = 3.97ILVRR152 pKa = 11.84EE153 pKa = 4.15EE154 pKa = 3.98TNDD157 pKa = 3.49ARR159 pKa = 11.84WIDD162 pKa = 4.46LPCGPAVSCVTLRR175 pKa = 11.84EE176 pKa = 4.09YY177 pKa = 11.23VIGAEE182 pKa = 4.14LTASGEE188 pKa = 4.13EE189 pKa = 4.34AKK191 pKa = 10.88LQMGQIQVYY200 pKa = 9.03VPFPTGPYY208 pKa = 8.31TAVFTLDD215 pKa = 3.21TAAINYY221 pKa = 8.13WGEE224 pKa = 3.94FSEE227 pKa = 4.16MTMAILRR234 pKa = 11.84SVSFADD240 pKa = 3.76PDD242 pKa = 3.93AEE244 pKa = 5.07ADD246 pKa = 3.58AAPTAGTGSGADD258 pKa = 3.32AGTDD262 pKa = 2.97AGTALPQQADD272 pKa = 3.44RR273 pKa = 4.81

Molecular weight: 28.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I1Z643|A0A1I1Z643_9ACTN NADP-dependent 3-hydroxy acid dehydrogenase YdfG OS=Streptomyces alni OX=380248 GN=SAMN05216251_102333 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILATRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.88GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7062

0

7062

2442735

27

6537

345.9

36.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.266 ± 0.046

0.735 ± 0.008

6.189 ± 0.023

5.014 ± 0.033

2.672 ± 0.015

9.798 ± 0.028

2.246 ± 0.013

3.093 ± 0.019

2.013 ± 0.022

10.034 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.637 ± 0.012

1.821 ± 0.019

6.278 ± 0.028

2.719 ± 0.016

7.806 ± 0.038

5.189 ± 0.028

6.509 ± 0.033

8.337 ± 0.023

1.509 ± 0.012

2.134 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski