Amino acid dipepetide frequency for Streptomyces alni

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	24.767AlaAla: 24.767 ± 0.187	1.014AlaCys: 1.014 ± 0.021	9.484AlaAsp: 9.484 ± 0.079	8.192AlaGlu: 8.192 ± 0.092	3.472AlaPhe: 3.472 ± 0.042	13.963AlaGly: 13.963 ± 0.101	2.943AlaHis: 2.943 ± 0.037	3.406AlaIle: 3.406 ± 0.042	2.862AlaLys: 2.862 ± 0.043	14.625AlaLeu: 14.625 ± 0.116	2.487AlaMet: 2.487 ± 0.028	2.128AlaAsn: 2.128 ± 0.032	7.866AlaPro: 7.866 ± 0.081	3.72AlaGln: 3.72 ± 0.045	10.23AlaArg: 10.23 ± 0.077	6.131AlaSer: 6.131 ± 0.058	7.58AlaThr: 7.58 ± 0.069	13.066AlaVal: 13.066 ± 0.09	1.844AlaTrp: 1.844 ± 0.029	2.882AlaTyr: 2.882 ± 0.04	0.0AlaXaa: 0.0 ± 0.0
Cys	1.033CysAla: 1.033 ± 0.025	0.086CysCys: 0.086 ± 0.007	0.477CysAsp: 0.477 ± 0.016	0.329CysGlu: 0.329 ± 0.012	0.194CysPhe: 0.194 ± 0.008	0.925CysGly: 0.925 ± 0.024	0.18CysHis: 0.18 ± 0.009	0.135CysIle: 0.135 ± 0.008	0.11CysLys: 0.11 ± 0.007	0.702CysLeu: 0.702 ± 0.019	0.109CysMet: 0.109 ± 0.006	0.147CysAsn: 0.147 ± 0.009	0.422CysPro: 0.422 ± 0.016	0.148CysGln: 0.148 ± 0.008	0.533CysArg: 0.533 ± 0.016	0.409CysSer: 0.409 ± 0.015	0.479CysThr: 0.479 ± 0.014	0.659CysVal: 0.659 ± 0.017	0.124CysTrp: 0.124 ± 0.008	0.149CysTyr: 0.149 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	8.307AspAla: 8.307 ± 0.068	0.436AspCys: 0.436 ± 0.016	3.734AspAsp: 3.734 ± 0.049	3.448AspGlu: 3.448 ± 0.045	1.687AspPhe: 1.687 ± 0.031	7.045AspGly: 7.045 ± 0.063	1.414AspHis: 1.414 ± 0.026	1.979AspIle: 1.979 ± 0.03	1.2AspLys: 1.2 ± 0.023	6.272AspLeu: 6.272 ± 0.061	0.753AspMet: 0.753 ± 0.018	1.042AspAsn: 1.042 ± 0.019	4.686AspPro: 4.686 ± 0.048	1.555AspGln: 1.555 ± 0.027	4.959AspArg: 4.959 ± 0.056	2.801AspSer: 2.801 ± 0.039	3.638AspThr: 3.638 ± 0.042	4.699AspVal: 4.699 ± 0.05	1.037AspTrp: 1.037 ± 0.021	1.199AspTyr: 1.199 ± 0.026	0.0AspXaa: 0.0 ± 0.0
Glu	6.485GluAla: 6.485 ± 0.08	0.31GluCys: 0.31 ± 0.013	2.52GluAsp: 2.52 ± 0.037	2.905GluGlu: 2.905 ± 0.044	1.308GluPhe: 1.308 ± 0.025	3.65GluGly: 3.65 ± 0.048	1.423GluHis: 1.423 ± 0.027	2.022GluIle: 2.022 ± 0.033	1.079GluLys: 1.079 ± 0.025	5.991GluLeu: 5.991 ± 0.063	0.776GluMet: 0.776 ± 0.019	0.861GluAsn: 0.861 ± 0.019	3.221GluPro: 3.221 ± 0.043	2.038GluGln: 2.038 ± 0.034	4.889GluArg: 4.889 ± 0.053	2.457GluSer: 2.457 ± 0.037	2.642GluThr: 2.642 ± 0.038	3.911GluVal: 3.911 ± 0.045	0.68GluTrp: 0.68 ± 0.018	0.973GluTyr: 0.973 ± 0.024	0.0GluXaa: 0.0 ± 0.0
Phe	3.683PheAla: 3.683 ± 0.044	0.262PheCys: 0.262 ± 0.01	1.941PheAsp: 1.941 ± 0.031	1.178PheGlu: 1.178 ± 0.021	0.851PhePhe: 0.851 ± 0.024	3.045PheGly: 3.045 ± 0.039	0.62PheHis: 0.62 ± 0.016	0.668PheIle: 0.668 ± 0.018	0.481PheLys: 0.481 ± 0.017	2.487PheLeu: 2.487 ± 0.035	0.375PheMet: 0.375 ± 0.012	0.582PheAsn: 0.582 ± 0.019	1.388PhePro: 1.388 ± 0.026	0.664PheGln: 0.664 ± 0.016	1.828PheArg: 1.828 ± 0.029	1.454PheSer: 1.454 ± 0.024	2.11PheThr: 2.11 ± 0.029	2.128PheVal: 2.128 ± 0.033	0.402PheTrp: 0.402 ± 0.014	0.57PheTyr: 0.57 ± 0.017	0.0PheXaa: 0.0 ± 0.0
Gly	12.127GlyAla: 12.127 ± 0.087	0.818GlyCys: 0.818 ± 0.018	5.484GlyAsp: 5.484 ± 0.05	4.571GlyGlu: 4.571 ± 0.049	2.859GlyPhe: 2.859 ± 0.04	9.853GlyGly: 9.853 ± 0.099	2.344GlyHis: 2.344 ± 0.035	3.368GlyIle: 3.368 ± 0.041	2.345GlyLys: 2.345 ± 0.035	9.369GlyLeu: 9.369 ± 0.079	1.888GlyMet: 1.888 ± 0.03	1.866GlyAsn: 1.866 ± 0.036	5.751GlyPro: 5.751 ± 0.056	2.742GlyGln: 2.742 ± 0.046	7.897GlyArg: 7.897 ± 0.066	5.866GlySer: 5.866 ± 0.059	7.284GlyThr: 7.284 ± 0.07	7.41GlyVal: 7.41 ± 0.065	1.751GlyTrp: 1.751 ± 0.026	2.393GlyTyr: 2.393 ± 0.041	0.0GlyXaa: 0.0 ± 0.0
His	2.719HisAla: 2.719 ± 0.037	0.177HisCys: 0.177 ± 0.009	1.403HisAsp: 1.403 ± 0.024	1.071HisGlu: 1.071 ± 0.022	0.68HisPhe: 0.68 ± 0.018	2.423HisGly: 2.423 ± 0.033	0.649HisHis: 0.649 ± 0.019	0.702HisIle: 0.702 ± 0.018	0.344HisLys: 0.344 ± 0.011	2.332HisLeu: 2.332 ± 0.036	0.322HisMet: 0.322 ± 0.012	0.386HisAsn: 0.386 ± 0.012	1.763HisPro: 1.763 ± 0.032	0.607HisGln: 0.607 ± 0.015	1.966HisArg: 1.966 ± 0.03	1.033HisSer: 1.033 ± 0.022	1.406HisThr: 1.406 ± 0.025	1.622HisVal: 1.622 ± 0.028	0.378HisTrp: 0.378 ± 0.011	0.481HisTyr: 0.481 ± 0.014	0.0HisXaa: 0.0 ± 0.0
Ile	4.736IleAla: 4.736 ± 0.041	0.276IleCys: 0.276 ± 0.012	2.197IleAsp: 2.197 ± 0.032	1.745IleGlu: 1.745 ± 0.03	0.705IlePhe: 0.705 ± 0.019	3.665IleGly: 3.665 ± 0.041	0.568IleHis: 0.568 ± 0.016	0.841IleIle: 0.841 ± 0.02	0.685IleLys: 0.685 ± 0.018	2.259IleLeu: 2.259 ± 0.034	0.417IleMet: 0.417 ± 0.013	0.71IleAsn: 0.71 ± 0.02	1.706IlePro: 1.706 ± 0.029	0.68IleGln: 0.68 ± 0.019	2.157IleArg: 2.157 ± 0.033	1.684IleSer: 1.684 ± 0.026	2.291IleThr: 2.291 ± 0.035	2.71IleVal: 2.71 ± 0.038	0.366IleTrp: 0.366 ± 0.013	0.537IleTyr: 0.537 ± 0.014	0.0IleXaa: 0.0 ± 0.0
Lys	2.897LysAla: 2.897 ± 0.038	0.121LysCys: 0.121 ± 0.009	1.29LysAsp: 1.29 ± 0.032	0.95LysGlu: 0.95 ± 0.026	0.443LysPhe: 0.443 ± 0.016	1.73LysGly: 1.73 ± 0.034	0.433LysHis: 0.433 ± 0.012	0.801LysIle: 0.801 ± 0.021	0.734LysLys: 0.734 ± 0.028	1.829LysLeu: 1.829 ± 0.032	0.345LysMet: 0.345 ± 0.011	0.515LysAsn: 0.515 ± 0.018	1.24LysPro: 1.24 ± 0.027	0.689LysGln: 0.689 ± 0.018	1.289LysArg: 1.289 ± 0.027	1.133LysSer: 1.133 ± 0.026	1.211LysThr: 1.211 ± 0.028	1.78LysVal: 1.78 ± 0.03	0.257LysTrp: 0.257 ± 0.012	0.447LysTyr: 0.447 ± 0.015	0.0LysXaa: 0.0 ± 0.0
Leu	15.103LeuAla: 15.103 ± 0.12	0.771LeuCys: 0.771 ± 0.021	6.809LeuAsp: 6.809 ± 0.059	4.028LeuGlu: 4.028 ± 0.045	2.496LeuPhe: 2.496 ± 0.036	9.081LeuGly: 9.081 ± 0.07	2.231LeuHis: 2.231 ± 0.027	3.201LeuIle: 3.201 ± 0.051	1.718LeuLys: 1.718 ± 0.03	11.016LeuLeu: 11.016 ± 0.104	1.528LeuMet: 1.528 ± 0.029	1.658LeuAsn: 1.658 ± 0.026	6.585LeuPro: 6.585 ± 0.06	2.025LeuGln: 2.025 ± 0.03	8.513LeuArg: 8.513 ± 0.073	5.207LeuSer: 5.207 ± 0.045	7.007LeuThr: 7.007 ± 0.059	8.265LeuVal: 8.265 ± 0.069	1.286LeuTrp: 1.286 ± 0.028	1.813LeuTyr: 1.813 ± 0.033	0.0LeuXaa: 0.0 ± 0.0
Met	2.137MetAla: 2.137 ± 0.032	0.127MetCys: 0.127 ± 0.007	0.848MetAsp: 0.848 ± 0.017	0.64MetGlu: 0.64 ± 0.018	0.433MetPhe: 0.433 ± 0.013	1.229MetGly: 1.229 ± 0.023	0.318MetHis: 0.318 ± 0.011	0.648MetIle: 0.648 ± 0.016	0.376MetLys: 0.376 ± 0.014	1.61MetLeu: 1.61 ± 0.025	0.272MetMet: 0.272 ± 0.011	0.414MetAsn: 0.414 ± 0.013	1.066MetPro: 1.066 ± 0.02	0.414MetGln: 0.414 ± 0.012	1.342MetArg: 1.342 ± 0.024	1.302MetSer: 1.302 ± 0.022	1.486MetThr: 1.486 ± 0.023	1.197MetVal: 1.197 ± 0.023	0.223MetTrp: 0.223 ± 0.009	0.29MetTyr: 0.29 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	2.358AsnAla: 2.358 ± 0.037	0.165AsnCys: 0.165 ± 0.008	1.006AsnAsp: 1.006 ± 0.023	0.728AsnGlu: 0.728 ± 0.017	0.499AsnPhe: 0.499 ± 0.016	2.22AsnGly: 2.22 ± 0.046	0.367AsnHis: 0.367 ± 0.012	0.658AsnIle: 0.658 ± 0.018	0.398AsnLys: 0.398 ± 0.014	1.625AsnLeu: 1.625 ± 0.029	0.263AsnMet: 0.263 ± 0.012	0.493AsnAsn: 0.493 ± 0.018	1.34AsnPro: 1.34 ± 0.029	0.535AsnGln: 0.535 ± 0.018	1.182AsnArg: 1.182 ± 0.024	1.084AsnSer: 1.084 ± 0.025	1.19AsnThr: 1.19 ± 0.027	1.345AsnVal: 1.345 ± 0.027	0.317AsnTrp: 0.317 ± 0.012	0.437AsnTyr: 0.437 ± 0.013	0.0AsnXaa: 0.0 ± 0.0
Pro	9.762ProAla: 9.762 ± 0.092	0.291ProCys: 0.291 ± 0.011	4.696ProAsp: 4.696 ± 0.055	3.892ProGlu: 3.892 ± 0.046	1.46ProPhe: 1.46 ± 0.026	7.148ProGly: 7.148 ± 0.074	1.388ProHis: 1.388 ± 0.027	1.274ProIle: 1.274 ± 0.027	1.127ProLys: 1.127 ± 0.025	5.357ProLeu: 5.357 ± 0.049	0.945ProMet: 0.945 ± 0.017	0.953ProAsn: 0.953 ± 0.021	3.946ProPro: 3.946 ± 0.074	1.814ProGln: 1.814 ± 0.034	4.056ProArg: 4.056 ± 0.047	3.309ProSer: 3.309 ± 0.043	3.459ProThr: 3.459 ± 0.048	5.535ProVal: 5.535 ± 0.046	0.876ProTrp: 0.876 ± 0.021	1.496ProTyr: 1.496 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	3.668GlnAla: 3.668 ± 0.05	0.158GlnCys: 0.158 ± 0.008	1.47GlnAsp: 1.47 ± 0.025	1.279GlnGlu: 1.279 ± 0.028	0.697GlnPhe: 0.697 ± 0.019	2.217GlnGly: 2.217 ± 0.034	0.673GlnHis: 0.673 ± 0.018	1.071GlnIle: 1.071 ± 0.023	0.575GlnLys: 0.575 ± 0.017	2.828GlnLeu: 2.828 ± 0.037	0.434GlnMet: 0.434 ± 0.015	0.558GlnAsn: 0.558 ± 0.017	1.715GlnPro: 1.715 ± 0.037	1.349GlnGln: 1.349 ± 0.038	2.26GlnArg: 2.26 ± 0.031	1.355GlnSer: 1.355 ± 0.025	1.4GlnThr: 1.4 ± 0.026	2.355GlnVal: 2.355 ± 0.034	0.509GlnTrp: 0.509 ± 0.016	0.617GlnTyr: 0.617 ± 0.018	0.0GlnXaa: 0.0 ± 0.0
Arg	9.936ArgAla: 9.936 ± 0.085	0.506ArgCys: 0.506 ± 0.016	4.316ArgAsp: 4.316 ± 0.048	4.201ArgGlu: 4.201 ± 0.055	2.184ArgPhe: 2.184 ± 0.032	5.807ArgGly: 5.807 ± 0.057	1.98ArgHis: 1.98 ± 0.029	3.207ArgIle: 3.207 ± 0.037	1.513ArgLys: 1.513 ± 0.026	8.592ArgLeu: 8.592 ± 0.08	1.658ArgMet: 1.658 ± 0.028	1.258ArgAsn: 1.258 ± 0.022	5.054ArgPro: 5.054 ± 0.058	2.27ArgGln: 2.27 ± 0.031	7.487ArgArg: 7.487 ± 0.072	3.828ArgSer: 3.828 ± 0.037	5.629ArgThr: 5.629 ± 0.053	5.518ArgVal: 5.518 ± 0.051	1.331ArgTrp: 1.331 ± 0.026	1.782ArgTyr: 1.782 ± 0.03	0.0ArgXaa: 0.0 ± 0.0
Ser	7.425SerAla: 7.425 ± 0.064	0.393SerCys: 0.393 ± 0.015	2.864SerAsp: 2.864 ± 0.038	2.065SerGlu: 2.065 ± 0.029	1.556SerPhe: 1.556 ± 0.029	6.645SerGly: 6.645 ± 0.063	0.998SerHis: 0.998 ± 0.023	1.372SerIle: 1.372 ± 0.029	0.995SerLys: 0.995 ± 0.024	4.585SerLeu: 4.585 ± 0.046	1.03SerMet: 1.03 ± 0.023	0.917SerAsn: 0.917 ± 0.022	3.183SerPro: 3.183 ± 0.04	1.258SerGln: 1.258 ± 0.025	3.565SerArg: 3.565 ± 0.043	3.056SerSer: 3.056 ± 0.055	3.37SerThr: 3.37 ± 0.046	4.356SerVal: 4.356 ± 0.046	0.926SerTrp: 0.926 ± 0.021	1.335SerTyr: 1.335 ± 0.031	0.0SerXaa: 0.0 ± 0.0
Thr	10.195ThrAla: 10.195 ± 0.069	0.426ThrCys: 0.426 ± 0.014	4.019ThrAsp: 4.019 ± 0.049	2.948ThrGlu: 2.948 ± 0.038	1.714ThrPhe: 1.714 ± 0.029	7.434ThrGly: 7.434 ± 0.067	1.238ThrHis: 1.238 ± 0.023	1.722ThrIle: 1.722 ± 0.032	1.217ThrLys: 1.217 ± 0.026	5.691ThrLeu: 5.691 ± 0.053	0.932ThrMet: 0.932 ± 0.022	1.098ThrAsn: 1.098 ± 0.027	4.438ThrPro: 4.438 ± 0.057	1.387ThrGln: 1.387 ± 0.03	3.803ThrArg: 3.803 ± 0.043	3.445ThrSer: 3.445 ± 0.053	4.374ThrThr: 4.374 ± 0.075	6.564ThrVal: 6.564 ± 0.063	0.919ThrTrp: 0.919 ± 0.021	1.52ThrTyr: 1.52 ± 0.035	0.0ThrXaa: 0.0 ± 0.0
Val	10.776ValAla: 10.776 ± 0.087	0.714ValCys: 0.714 ± 0.019	4.861ValAsp: 4.861 ± 0.048	4.147ValGlu: 4.147 ± 0.052	2.333ValPhe: 2.333 ± 0.033	6.498ValGly: 6.498 ± 0.057	1.913ValHis: 1.913 ± 0.03	2.83ValIle: 2.83 ± 0.036	1.663ValLys: 1.663 ± 0.032	9.412ValLeu: 9.412 ± 0.081	1.337ValMet: 1.337 ± 0.024	1.708ValAsn: 1.708 ± 0.028	5.489ValPro: 5.489 ± 0.054	2.019ValGln: 2.019 ± 0.031	6.965ValArg: 6.965 ± 0.067	4.308ValSer: 4.308 ± 0.042	6.071ValThr: 6.071 ± 0.057	7.634ValVal: 7.634 ± 0.07	1.132ValTrp: 1.132 ± 0.022	1.554ValTyr: 1.554 ± 0.026	0.0ValXaa: 0.0 ± 0.0
Trp	1.659TrpAla: 1.659 ± 0.025	0.136TrpCys: 0.136 ± 0.007	0.851TrpAsp: 0.851 ± 0.021	0.681TrpGlu: 0.681 ± 0.016	0.497TrpPhe: 0.497 ± 0.016	1.014TrpGly: 1.014 ± 0.021	0.386TrpHis: 0.386 ± 0.013	0.533TrpIle: 0.533 ± 0.015	0.342TrpLys: 0.342 ± 0.014	1.695TrpLeu: 1.695 ± 0.035	0.269TrpMet: 0.269 ± 0.01	0.449TrpAsn: 0.449 ± 0.017	0.809TrpPro: 0.809 ± 0.019	0.625TrpGln: 0.625 ± 0.016	1.284TrpArg: 1.284 ± 0.026	1.001TrpSer: 1.001 ± 0.017	1.167TrpThr: 1.167 ± 0.023	0.942TrpVal: 0.942 ± 0.021	0.353TrpTrp: 0.353 ± 0.013	0.393TrpTyr: 0.393 ± 0.011	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.892TyrAla: 2.892 ± 0.036	0.163TyrCys: 0.163 ± 0.009	1.618TyrAsp: 1.618 ± 0.036	1.142TyrGlu: 1.142 ± 0.025	0.65TyrPhe: 0.65 ± 0.015	2.392TyrGly: 2.392 ± 0.034	0.398TyrHis: 0.398 ± 0.014	0.495TyrIle: 0.495 ± 0.015	0.37TyrLys: 0.37 ± 0.016	2.066TyrLeu: 2.066 ± 0.03	0.233TyrMet: 0.233 ± 0.01	0.469TyrAsn: 0.469 ± 0.019	1.114TyrPro: 1.114 ± 0.022	0.651TyrGln: 0.651 ± 0.019	1.852TyrArg: 1.852 ± 0.028	1.03TyrSer: 1.03 ± 0.025	1.292TyrThr: 1.292 ± 0.026	1.67TyrVal: 1.67 ± 0.03	0.373TyrTrp: 0.373 ± 0.013	0.469TyrTyr: 0.469 ± 0.015	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.004XaaXaa: 0.004 ± 0.003

Statistics based on 7062 proteins (2442735 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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