McMurdo Ice Shelf pond-associated circular DNA virus-4

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 7.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A075LYS2|A0A075LYS2_9VIRU Replication-associated protein OS=McMurdo Ice Shelf pond-associated circular DNA virus-4 OX=1521388 PE=4 SV=1
MM1 pKa = 7.3SACEE5 pKa = 3.81TGGVLVHH12 pKa = 6.94AEE14 pKa = 4.12GLASVHH20 pKa = 6.2SPGVVDD26 pKa = 4.85FDD28 pKa = 3.99VLFVCDD34 pKa = 3.1VRR36 pKa = 11.84TRR38 pKa = 11.84VAEE41 pKa = 3.97LKK43 pKa = 10.45VRR45 pKa = 11.84SEE47 pKa = 4.16RR48 pKa = 11.84LVVSEE53 pKa = 4.55DD54 pKa = 3.43TSDD57 pKa = 3.48DD58 pKa = 3.15KK59 pKa = 11.4HH60 pKa = 7.17RR61 pKa = 11.84KK62 pKa = 8.83VLTSVEE68 pKa = 3.98FLQVCIRR75 pKa = 11.84QVRR78 pKa = 11.84VVVVFHH84 pKa = 7.59DD85 pKa = 3.83SGLGKK90 pKa = 10.26LVEE93 pKa = 4.08QLEE96 pKa = 4.38RR97 pKa = 11.84RR98 pKa = 11.84SWFHH102 pKa = 5.47VVRR105 pKa = 11.84LHH107 pKa = 7.45LGDD110 pKa = 5.62LEE112 pKa = 4.54VLDD115 pKa = 4.59KK116 pKa = 11.52LDD118 pKa = 3.58VLEE121 pKa = 4.1QTVRR125 pKa = 11.84LGDD128 pKa = 3.74SAGGTLYY135 pKa = 11.06LVDD138 pKa = 4.42SVHH141 pKa = 7.2SSVTEE146 pKa = 3.97TVVVSQKK153 pKa = 10.76AALNNRR159 pKa = 11.84LEE161 pKa = 4.45CAEE164 pKa = 3.98GRR166 pKa = 11.84TDD168 pKa = 3.27LHH170 pKa = 7.5RR171 pKa = 11.84GDD173 pKa = 4.39VLADD177 pKa = 3.39SAAVVYY183 pKa = 9.96HH184 pKa = 6.31ISSMVRR190 pKa = 11.84HH191 pKa = 5.82VGYY194 pKa = 8.57LTGNLVRR201 pKa = 11.84EE202 pKa = 4.26AGIPRR207 pKa = 11.84CLGSIDD213 pKa = 3.5VVGDD217 pKa = 3.1VRR219 pKa = 11.84EE220 pKa = 3.99NVGIVRR226 pKa = 11.84RR227 pKa = 11.84HH228 pKa = 3.82QQGRR232 pKa = 11.84ARR234 pKa = 11.84CLTVGVVLAVPVAHH248 pKa = 6.2QCPCGGAVDD257 pKa = 5.35LIEE260 pKa = 5.1IEE262 pKa = 4.46EE263 pKa = 4.14VHH265 pKa = 5.9TRR267 pKa = 11.84CLVRR271 pKa = 11.84IDD273 pKa = 3.89GLVLSSYY280 pKa = 10.82DD281 pKa = 3.44VQNALSEE288 pKa = 4.51SSASSGGSSLVGAGGALAGAEE309 pKa = 4.19LSCRR313 pKa = 11.84SSTT316 pKa = 3.97

Molecular weight: 33.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A075LZ76|A0A075LZ76_9VIRU Uncharacterized protein OS=McMurdo Ice Shelf pond-associated circular DNA virus-4 OX=1521388 PE=4 SV=1
MM1 pKa = 6.94YY2 pKa = 10.17RR3 pKa = 11.84PRR5 pKa = 11.84RR6 pKa = 11.84TTNVGPTGSNPGLAIQRR23 pKa = 11.84LVFGSRR29 pKa = 11.84ARR31 pKa = 11.84EE32 pKa = 3.73EE33 pKa = 4.33DD34 pKa = 3.7SPAVSQGSQAKK45 pKa = 9.34KK46 pKa = 9.78RR47 pKa = 11.84RR48 pKa = 11.84LSRR51 pKa = 11.84SGGWAKK57 pKa = 10.53EE58 pKa = 3.6NRR60 pKa = 11.84TRR62 pKa = 11.84IPLYY66 pKa = 10.28SSDD69 pKa = 3.59MAKK72 pKa = 10.54SSIKK76 pKa = 10.3RR77 pKa = 11.84SGSRR81 pKa = 11.84IAKK84 pKa = 9.25RR85 pKa = 11.84RR86 pKa = 11.84YY87 pKa = 7.09TKK89 pKa = 10.45RR90 pKa = 11.84STKK93 pKa = 10.01GRR95 pKa = 11.84TRR97 pKa = 11.84RR98 pKa = 11.84PLSRR102 pKa = 11.84ARR104 pKa = 11.84PTRR107 pKa = 11.84KK108 pKa = 8.94FRR110 pKa = 11.84TRR112 pKa = 11.84KK113 pKa = 9.9SPARR117 pKa = 11.84SYY119 pKa = 10.98KK120 pKa = 9.93RR121 pKa = 11.84RR122 pKa = 11.84STGGRR127 pKa = 11.84GGFAKK132 pKa = 10.17RR133 pKa = 11.84VLNVVTRR140 pKa = 11.84QNQAINTYY148 pKa = 8.0KK149 pKa = 10.2TSRR152 pKa = 11.84VDD154 pKa = 5.5FFDD157 pKa = 4.62LNQVDD162 pKa = 4.1GSTARR167 pKa = 11.84ALVCYY172 pKa = 9.8RR173 pKa = 11.84NCKK176 pKa = 10.22NYY178 pKa = 10.92ANGQAPGTALLMPTDD193 pKa = 4.84DD194 pKa = 3.93PHH196 pKa = 8.83ILTDD200 pKa = 3.33IANNIDD206 pKa = 3.21ASKK209 pKa = 6.81TTRR212 pKa = 11.84YY213 pKa = 7.52TRR215 pKa = 11.84LAYY218 pKa = 9.42KK219 pKa = 10.67VSGKK223 pKa = 7.22VTNMSNHH230 pKa = 6.15AADD233 pKa = 3.65VVHH236 pKa = 5.63YY237 pKa = 9.28RR238 pKa = 11.84CRR240 pKa = 11.84VRR242 pKa = 11.84KK243 pKa = 9.41DD244 pKa = 3.32VPPVQVGSTLGTFQTIVQSGFLGNNNGLGNAAVDD278 pKa = 4.27GVNQVQGASCTIPQAYY294 pKa = 10.2SLFEE298 pKa = 3.56NVQFVKK304 pKa = 10.33HH305 pKa = 5.84FKK307 pKa = 7.46ITKK310 pKa = 9.03VKK312 pKa = 7.12THH314 pKa = 5.82HH315 pKa = 6.49MEE317 pKa = 4.2PASSFKK323 pKa = 10.85LFYY326 pKa = 10.45KK327 pKa = 10.43LSKK330 pKa = 9.87PRR332 pKa = 11.84VVKK335 pKa = 10.99HH336 pKa = 6.26NDD338 pKa = 3.05YY339 pKa = 11.11TNLTNAYY346 pKa = 9.29LQEE349 pKa = 4.56LYY351 pKa = 10.55GGQYY355 pKa = 10.22FSVFVIRR362 pKa = 11.84GVLGYY367 pKa = 10.57DD368 pKa = 3.0KK369 pKa = 11.15SLAANLQLGYY379 pKa = 9.11TGADD383 pKa = 2.68IAYY386 pKa = 6.81EE387 pKa = 3.68QHH389 pKa = 7.13IEE391 pKa = 3.96VDD393 pKa = 4.03YY394 pKa = 9.63TWTVDD399 pKa = 3.1GSKK402 pKa = 10.83AFGMNQYY409 pKa = 7.51TTGLARR415 pKa = 11.84GHH417 pKa = 5.76VPAPIVEE424 pKa = 4.23NFNQNVVVYY433 pKa = 10.6NDD435 pKa = 3.68GQAKK439 pKa = 7.8FTTGSIGQTAWSTNPDD455 pKa = 4.48PIVEE459 pKa = 5.26DD460 pKa = 4.18GDD462 pKa = 3.67QQ463 pKa = 3.24

Molecular weight: 51.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1127

316

463

375.7

41.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.808 ± 0.359

1.331 ± 0.665

5.768 ± 0.616

4.525 ± 1.165

2.484 ± 0.584

7.986 ± 0.651

2.928 ± 0.527

3.106 ± 0.288

5.146 ± 1.161

7.453 ± 1.427

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.42 ± 0.365

3.904 ± 1.286

4.348 ± 1.279

4.082 ± 0.506

8.075 ± 0.413

7.453 ± 0.873

6.921 ± 1.015

9.76 ± 2.667

1.331 ± 0.698

4.17 ± 1.072
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski