Rhizobium phage RR1-B

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9U430|R9U430_9CAUD AAA domain-containing protein OS=Rhizobium phage RR1-B OX=929834 GN=RHYG_00008 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 10.16ALLSSYY8 pKa = 10.09PIVADD13 pKa = 3.57IDD15 pKa = 3.65DD16 pKa = 4.19AEE18 pKa = 4.14IRR20 pKa = 11.84NAIEE24 pKa = 4.23GFASEE29 pKa = 5.34LEE31 pKa = 4.04MAEE34 pKa = 4.21RR35 pKa = 11.84AEE37 pKa = 4.14SAANIWYY44 pKa = 9.68EE45 pKa = 3.82EE46 pKa = 4.14TFYY49 pKa = 11.01EE50 pKa = 4.81FPVDD54 pKa = 2.88IHH56 pKa = 6.29FRR58 pKa = 11.84AGQIAAFVSGAISMARR74 pKa = 11.84DD75 pKa = 3.5LGHH78 pKa = 7.21LPEE81 pKa = 5.11GCAAA85 pKa = 4.37

Molecular weight: 9.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9U0S7|R9U0S7_9CAUD Uncharacterized protein OS=Rhizobium phage RR1-B OX=929834 GN=RHYG_00014 PE=4 SV=1
MM1 pKa = 6.97QRR3 pKa = 11.84DD4 pKa = 3.8GSTLHH9 pKa = 6.84FDD11 pKa = 4.85RR12 pKa = 11.84IFEE15 pKa = 4.46ALDD18 pKa = 3.19GTACLTLDD26 pKa = 3.49TLEE29 pKa = 4.56EE30 pKa = 4.24KK31 pKa = 10.79SGLTRR36 pKa = 11.84TQLARR41 pKa = 11.84VTAKK45 pKa = 9.66MVTAALIEE53 pKa = 4.0RR54 pKa = 11.84RR55 pKa = 11.84KK56 pKa = 9.08MGCYY60 pKa = 9.58QLTAVGQKK68 pKa = 10.23AKK70 pKa = 9.55RR71 pKa = 11.84TGNIPMPVQPIRR83 pKa = 11.84PAAPPSDD90 pKa = 3.93SFRR93 pKa = 11.84QRR95 pKa = 11.84LWSIMRR101 pKa = 11.84MSGTFMAAEE110 pKa = 4.2LVMAANWPLKK120 pKa = 10.26RR121 pKa = 11.84PEE123 pKa = 4.05FEE125 pKa = 3.94AGKK128 pKa = 10.61YY129 pKa = 10.17LLALKK134 pKa = 10.28RR135 pKa = 11.84AGYY138 pKa = 10.05LIEE141 pKa = 4.68LPKK144 pKa = 10.73GPRR147 pKa = 11.84GQMRR151 pKa = 11.84YY152 pKa = 9.87RR153 pKa = 11.84LVRR156 pKa = 11.84NSGLLAPVVSSVDD169 pKa = 3.26GSVYY173 pKa = 10.8DD174 pKa = 3.9PNTRR178 pKa = 11.84EE179 pKa = 4.25AMPCAKK185 pKa = 9.93QAA187 pKa = 3.29

Molecular weight: 20.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

11458

53

826

220.3

24.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.568 ± 0.645

0.628 ± 0.105

5.786 ± 0.254

6.572 ± 0.363

3.657 ± 0.184

7.322 ± 0.494

1.702 ± 0.133

5.577 ± 0.178

5.132 ± 0.259

8.562 ± 0.267

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.583 ± 0.174

3.212 ± 0.203

4.73 ± 0.289

3.055 ± 0.159

6.336 ± 0.358

6.162 ± 0.284

5.813 ± 0.288

6.825 ± 0.275

1.501 ± 0.137

2.278 ± 0.163
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski