Amino acid dipepetide frequency for Rhizobium phage RR1-B

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.377AlaAla: 19.377 ± 2.561
0.524AlaCys: 0.524 ± 0.2
6.808AlaAsp: 6.808 ± 0.774
8.641AlaGlu: 8.641 ± 0.827
3.404AlaPhe: 3.404 ± 0.465
10.387AlaGly: 10.387 ± 1.464
1.309AlaHis: 1.309 ± 0.362
6.895AlaIle: 6.895 ± 0.738
7.768AlaLys: 7.768 ± 0.979
11.434AlaLeu: 11.434 ± 1.184
4.539AlaMet: 4.539 ± 0.72
4.015AlaAsn: 4.015 ± 0.69
4.888AlaPro: 4.888 ± 0.651
3.666AlaGln: 3.666 ± 0.52
6.895AlaArg: 6.895 ± 0.661
6.459AlaSer: 6.459 ± 1.036
6.895AlaThr: 6.895 ± 0.998
8.205AlaVal: 8.205 ± 0.931
1.309AlaTrp: 1.309 ± 0.403
2.182AlaTyr: 2.182 ± 0.472
0.0AlaXaa: 0.0 ± 0.0
Cys
0.349CysAla: 0.349 ± 0.184
0.087CysCys: 0.087 ± 0.08
0.349CysAsp: 0.349 ± 0.16
0.698CysGlu: 0.698 ± 0.255
0.262CysPhe: 0.262 ± 0.201
0.611CysGly: 0.611 ± 0.255
0.087CysHis: 0.087 ± 0.077
0.349CysIle: 0.349 ± 0.2
0.524CysLys: 0.524 ± 0.219
0.786CysLeu: 0.786 ± 0.327
0.087CysMet: 0.087 ± 0.086
0.0CysAsn: 0.0 ± 0.0
0.349CysPro: 0.349 ± 0.207
0.262CysGln: 0.262 ± 0.152
0.175CysArg: 0.175 ± 0.118
0.349CysSer: 0.349 ± 0.16
0.175CysThr: 0.175 ± 0.113
0.436CysVal: 0.436 ± 0.218
0.0CysTrp: 0.0 ± 0.0
0.349CysTyr: 0.349 ± 0.158
0.0CysXaa: 0.0 ± 0.0
Asp
7.506AspAla: 7.506 ± 0.803
0.349AspCys: 0.349 ± 0.17
2.968AspAsp: 2.968 ± 0.642
3.142AspGlu: 3.142 ± 0.562
3.055AspPhe: 3.055 ± 0.564
5.499AspGly: 5.499 ± 0.554
0.873AspHis: 0.873 ± 0.232
3.491AspIle: 3.491 ± 0.481
1.92AspLys: 1.92 ± 0.439
6.721AspLeu: 6.721 ± 1.019
1.571AspMet: 1.571 ± 0.405
0.611AspAsn: 0.611 ± 0.236
3.753AspPro: 3.753 ± 0.552
1.309AspGln: 1.309 ± 0.287
4.015AspArg: 4.015 ± 0.555
1.92AspSer: 1.92 ± 0.359
3.055AspThr: 3.055 ± 0.702
3.928AspVal: 3.928 ± 0.518
0.96AspTrp: 0.96 ± 0.256
1.222AspTyr: 1.222 ± 0.321
0.0AspXaa: 0.0 ± 0.0
Glu
8.816GluAla: 8.816 ± 0.8
0.436GluCys: 0.436 ± 0.197
3.317GluAsp: 3.317 ± 0.493
4.102GluGlu: 4.102 ± 0.677
2.88GluPhe: 2.88 ± 0.371
3.753GluGly: 3.753 ± 0.484
1.135GluHis: 1.135 ± 0.319
4.626GluIle: 4.626 ± 0.607
4.102GluLys: 4.102 ± 0.61
4.801GluLeu: 4.801 ± 0.705
2.095GluMet: 2.095 ± 0.514
3.229GluAsn: 3.229 ± 0.524
2.182GluPro: 2.182 ± 0.379
2.182GluGln: 2.182 ± 0.418
4.539GluArg: 4.539 ± 0.744
1.571GluSer: 1.571 ± 0.351
5.062GluThr: 5.062 ± 0.602
3.928GluVal: 3.928 ± 0.644
1.222GluTrp: 1.222 ± 0.353
1.746GluTyr: 1.746 ± 0.32
0.0GluXaa: 0.0 ± 0.0
Phe
5.062PheAla: 5.062 ± 0.678
0.262PheCys: 0.262 ± 0.151
2.618PheAsp: 2.618 ± 0.36
2.618PheGlu: 2.618 ± 0.406
1.135PhePhe: 1.135 ± 0.296
3.317PheGly: 3.317 ± 0.543
0.611PheHis: 0.611 ± 0.202
2.269PheIle: 2.269 ± 0.456
1.047PheLys: 1.047 ± 0.268
2.269PheLeu: 2.269 ± 0.395
0.524PheMet: 0.524 ± 0.216
1.047PheAsn: 1.047 ± 0.253
2.182PhePro: 2.182 ± 0.369
1.833PheGln: 1.833 ± 0.321
2.357PheArg: 2.357 ± 0.473
2.706PheSer: 2.706 ± 0.525
1.135PheThr: 1.135 ± 0.261
1.92PheVal: 1.92 ± 0.354
0.786PheTrp: 0.786 ± 0.225
0.873PheTyr: 0.873 ± 0.275
0.0PheXaa: 0.0 ± 0.0
Gly
7.768GlyAla: 7.768 ± 1.111
0.524GlyCys: 0.524 ± 0.223
4.015GlyAsp: 4.015 ± 0.734
4.713GlyGlu: 4.713 ± 0.672
3.055GlyPhe: 3.055 ± 0.353
6.459GlyGly: 6.459 ± 1.374
1.135GlyHis: 1.135 ± 0.281
3.579GlyIle: 3.579 ± 0.491
4.277GlyLys: 4.277 ± 0.682
5.673GlyLeu: 5.673 ± 0.67
2.182GlyMet: 2.182 ± 0.409
2.357GlyAsn: 2.357 ± 0.494
1.833GlyPro: 1.833 ± 0.41
2.444GlyGln: 2.444 ± 0.413
4.364GlyArg: 4.364 ± 0.695
5.062GlySer: 5.062 ± 0.736
5.15GlyThr: 5.15 ± 0.953
5.412GlyVal: 5.412 ± 0.761
1.309GlyTrp: 1.309 ± 0.319
1.92GlyTyr: 1.92 ± 0.414
0.0GlyXaa: 0.0 ± 0.0
His
2.357HisAla: 2.357 ± 0.532
0.087HisCys: 0.087 ± 0.084
1.397HisAsp: 1.397 ± 0.359
0.698HisGlu: 0.698 ± 0.284
0.698HisPhe: 0.698 ± 0.192
1.746HisGly: 1.746 ± 0.408
0.087HisHis: 0.087 ± 0.077
0.611HisIle: 0.611 ± 0.204
1.222HisLys: 1.222 ± 0.356
1.833HisLeu: 1.833 ± 0.323
0.436HisMet: 0.436 ± 0.243
0.175HisAsn: 0.175 ± 0.115
0.524HisPro: 0.524 ± 0.219
0.873HisGln: 0.873 ± 0.227
1.135HisArg: 1.135 ± 0.328
0.786HisSer: 0.786 ± 0.275
0.611HisThr: 0.611 ± 0.205
0.873HisVal: 0.873 ± 0.258
0.262HisTrp: 0.262 ± 0.155
0.611HisTyr: 0.611 ± 0.258
0.0HisXaa: 0.0 ± 0.0
Ile
8.205IleAla: 8.205 ± 0.879
0.436IleCys: 0.436 ± 0.188
3.928IleAsp: 3.928 ± 0.517
5.15IleGlu: 5.15 ± 0.698
1.746IlePhe: 1.746 ± 0.351
2.88IleGly: 2.88 ± 0.59
1.047IleHis: 1.047 ± 0.292
2.008IleIle: 2.008 ± 0.576
2.269IleLys: 2.269 ± 0.388
4.277IleLeu: 4.277 ± 0.563
1.309IleMet: 1.309 ± 0.331
2.269IleAsn: 2.269 ± 0.54
2.618IlePro: 2.618 ± 0.422
1.484IleGln: 1.484 ± 0.367
3.579IleArg: 3.579 ± 0.552
4.626IleSer: 4.626 ± 0.551
3.055IleThr: 3.055 ± 0.511
3.055IleVal: 3.055 ± 0.629
0.436IleTrp: 0.436 ± 0.173
1.397IleTyr: 1.397 ± 0.284
0.0IleXaa: 0.0 ± 0.0
Lys
7.594LysAla: 7.594 ± 0.93
0.087LysCys: 0.087 ± 0.081
1.92LysAsp: 1.92 ± 0.451
2.531LysGlu: 2.531 ± 0.752
1.833LysPhe: 1.833 ± 0.383
3.404LysGly: 3.404 ± 0.416
0.698LysHis: 0.698 ± 0.215
3.055LysIle: 3.055 ± 0.448
2.182LysLys: 2.182 ± 0.451
4.975LysLeu: 4.975 ± 0.655
1.047LysMet: 1.047 ± 0.286
1.833LysAsn: 1.833 ± 0.381
3.142LysPro: 3.142 ± 0.535
1.309LysGln: 1.309 ± 0.316
3.753LysArg: 3.753 ± 0.596
3.753LysSer: 3.753 ± 0.816
3.055LysThr: 3.055 ± 0.459
3.579LysVal: 3.579 ± 0.518
0.524LysTrp: 0.524 ± 0.221
1.047LysTyr: 1.047 ± 0.356
0.0LysXaa: 0.0 ± 0.0
Leu
9.252LeuAla: 9.252 ± 1.328
0.262LeuCys: 0.262 ± 0.154
4.975LeuAsp: 4.975 ± 0.604
6.197LeuGlu: 6.197 ± 0.654
2.793LeuPhe: 2.793 ± 0.408
5.586LeuGly: 5.586 ± 0.747
1.484LeuHis: 1.484 ± 0.273
4.277LeuIle: 4.277 ± 0.628
5.324LeuLys: 5.324 ± 0.66
7.419LeuLeu: 7.419 ± 0.834
1.658LeuMet: 1.658 ± 0.402
2.793LeuAsn: 2.793 ± 0.408
5.324LeuPro: 5.324 ± 0.759
2.357LeuGln: 2.357 ± 0.458
5.15LeuArg: 5.15 ± 0.563
6.808LeuSer: 6.808 ± 0.934
6.372LeuThr: 6.372 ± 0.785
5.237LeuVal: 5.237 ± 0.647
1.484LeuTrp: 1.484 ± 0.429
0.873LeuTyr: 0.873 ± 0.228
0.0LeuXaa: 0.0 ± 0.0
Met
3.928MetAla: 3.928 ± 0.721
0.087MetCys: 0.087 ± 0.08
1.833MetAsp: 1.833 ± 0.351
2.008MetGlu: 2.008 ± 0.466
0.436MetPhe: 0.436 ± 0.189
1.658MetGly: 1.658 ± 0.322
0.524MetHis: 0.524 ± 0.215
1.309MetIle: 1.309 ± 0.309
1.135MetLys: 1.135 ± 0.303
1.397MetLeu: 1.397 ± 0.262
0.786MetMet: 0.786 ± 0.216
0.873MetAsn: 0.873 ± 0.222
1.571MetPro: 1.571 ± 0.361
0.873MetGln: 0.873 ± 0.295
2.269MetArg: 2.269 ± 0.494
1.92MetSer: 1.92 ± 0.361
1.658MetThr: 1.658 ± 0.373
1.397MetVal: 1.397 ± 0.315
0.087MetTrp: 0.087 ± 0.078
0.087MetTyr: 0.087 ± 0.085
0.0MetXaa: 0.0 ± 0.0
Asn
3.928AsnAla: 3.928 ± 0.632
0.087AsnCys: 0.087 ± 0.081
2.357AsnAsp: 2.357 ± 0.365
1.571AsnGlu: 1.571 ± 0.254
1.135AsnPhe: 1.135 ± 0.364
2.618AsnGly: 2.618 ± 0.513
0.611AsnHis: 0.611 ± 0.252
1.484AsnIle: 1.484 ± 0.299
1.484AsnLys: 1.484 ± 0.313
2.095AsnLeu: 2.095 ± 0.434
0.436AsnMet: 0.436 ± 0.178
0.96AsnAsn: 0.96 ± 0.233
2.968AsnPro: 2.968 ± 0.569
1.047AsnGln: 1.047 ± 0.251
2.531AsnArg: 2.531 ± 0.378
1.484AsnSer: 1.484 ± 0.327
1.833AsnThr: 1.833 ± 0.358
2.444AsnVal: 2.444 ± 0.434
0.524AsnTrp: 0.524 ± 0.182
0.524AsnTyr: 0.524 ± 0.256
0.0AsnXaa: 0.0 ± 0.0
Pro
5.761ProAla: 5.761 ± 0.822
0.349ProCys: 0.349 ± 0.24
2.793ProAsp: 2.793 ± 0.7
4.19ProGlu: 4.19 ± 0.736
1.571ProPhe: 1.571 ± 0.375
2.793ProGly: 2.793 ± 0.588
1.047ProHis: 1.047 ± 0.346
2.793ProIle: 2.793 ± 0.462
2.618ProLys: 2.618 ± 0.439
3.055ProLeu: 3.055 ± 0.679
0.873ProMet: 0.873 ± 0.199
1.746ProAsn: 1.746 ± 0.48
2.095ProPro: 2.095 ± 0.371
1.484ProGln: 1.484 ± 0.355
1.92ProArg: 1.92 ± 0.372
3.491ProSer: 3.491 ± 0.449
2.095ProThr: 2.095 ± 0.461
4.451ProVal: 4.451 ± 0.636
0.698ProTrp: 0.698 ± 0.254
1.484ProTyr: 1.484 ± 0.336
0.0ProXaa: 0.0 ± 0.0
Gln
3.666GlnAla: 3.666 ± 0.518
0.175GlnCys: 0.175 ± 0.126
0.873GlnAsp: 0.873 ± 0.266
1.309GlnGlu: 1.309 ± 0.304
1.222GlnPhe: 1.222 ± 0.384
1.571GlnGly: 1.571 ± 0.379
0.873GlnHis: 0.873 ± 0.259
2.182GlnIle: 2.182 ± 0.385
1.92GlnLys: 1.92 ± 0.467
2.618GlnLeu: 2.618 ± 0.482
0.524GlnMet: 0.524 ± 0.18
1.222GlnAsn: 1.222 ± 0.383
1.746GlnPro: 1.746 ± 0.42
1.135GlnGln: 1.135 ± 0.383
2.531GlnArg: 2.531 ± 0.379
2.095GlnSer: 2.095 ± 0.442
2.008GlnThr: 2.008 ± 0.434
1.484GlnVal: 1.484 ± 0.402
0.698GlnTrp: 0.698 ± 0.195
0.698GlnTyr: 0.698 ± 0.248
0.0GlnXaa: 0.0 ± 0.0
Arg
6.895ArgAla: 6.895 ± 0.696
0.436ArgCys: 0.436 ± 0.208
4.277ArgAsp: 4.277 ± 0.616
4.801ArgGlu: 4.801 ± 0.609
2.095ArgPhe: 2.095 ± 0.496
3.84ArgGly: 3.84 ± 0.413
1.571ArgHis: 1.571 ± 0.38
4.015ArgIle: 4.015 ± 0.505
3.404ArgLys: 3.404 ± 0.579
6.721ArgLeu: 6.721 ± 1.047
1.92ArgMet: 1.92 ± 0.395
1.571ArgAsn: 1.571 ± 0.548
2.531ArgPro: 2.531 ± 0.466
1.571ArgGln: 1.571 ± 0.365
4.451ArgArg: 4.451 ± 0.583
3.229ArgSer: 3.229 ± 0.594
2.793ArgThr: 2.793 ± 0.391
3.753ArgVal: 3.753 ± 0.512
1.309ArgTrp: 1.309 ± 0.315
2.182ArgTyr: 2.182 ± 0.417
0.0ArgXaa: 0.0 ± 0.0
Ser
6.983SerAla: 6.983 ± 0.911
0.524SerCys: 0.524 ± 0.278
4.19SerAsp: 4.19 ± 0.589
3.579SerGlu: 3.579 ± 0.607
3.229SerPhe: 3.229 ± 0.653
6.197SerGly: 6.197 ± 0.781
0.873SerHis: 0.873 ± 0.267
3.055SerIle: 3.055 ± 0.466
2.618SerLys: 2.618 ± 0.422
4.713SerLeu: 4.713 ± 0.62
1.658SerMet: 1.658 ± 0.351
1.658SerAsn: 1.658 ± 0.384
2.618SerPro: 2.618 ± 0.431
1.658SerGln: 1.658 ± 0.35
3.666SerArg: 3.666 ± 0.585
4.539SerSer: 4.539 ± 0.666
2.968SerThr: 2.968 ± 0.444
4.364SerVal: 4.364 ± 0.581
0.786SerTrp: 0.786 ± 0.225
1.746SerTyr: 1.746 ± 0.408
0.0SerXaa: 0.0 ± 0.0
Thr
8.117ThrAla: 8.117 ± 0.832
0.349ThrCys: 0.349 ± 0.178
2.793ThrAsp: 2.793 ± 0.511
2.269ThrGlu: 2.269 ± 0.411
2.793ThrPhe: 2.793 ± 0.434
4.888ThrGly: 4.888 ± 0.69
1.484ThrHis: 1.484 ± 0.386
4.015ThrIle: 4.015 ± 0.624
1.92ThrLys: 1.92 ± 0.336
5.673ThrLeu: 5.673 ± 0.682
1.397ThrMet: 1.397 ± 0.433
1.92ThrAsn: 1.92 ± 0.442
2.357ThrPro: 2.357 ± 0.42
1.484ThrGln: 1.484 ± 0.327
2.618ThrArg: 2.618 ± 0.359
3.055ThrSer: 3.055 ± 0.564
2.88ThrThr: 2.88 ± 0.436
5.848ThrVal: 5.848 ± 0.82
0.524ThrTrp: 0.524 ± 0.166
1.746ThrTyr: 1.746 ± 0.324
0.0ThrXaa: 0.0 ± 0.0
Val
7.332ValAla: 7.332 ± 0.838
0.611ValCys: 0.611 ± 0.243
4.102ValAsp: 4.102 ± 0.547
4.364ValGlu: 4.364 ± 0.668
1.92ValPhe: 1.92 ± 0.503
3.928ValGly: 3.928 ± 0.519
0.96ValHis: 0.96 ± 0.285
4.19ValIle: 4.19 ± 0.639
3.666ValLys: 3.666 ± 0.531
5.761ValLeu: 5.761 ± 0.659
1.571ValMet: 1.571 ± 0.339
2.269ValAsn: 2.269 ± 0.617
2.88ValPro: 2.88 ± 0.495
2.269ValGln: 2.269 ± 0.464
4.015ValArg: 4.015 ± 0.554
5.237ValSer: 5.237 ± 0.773
5.15ValThr: 5.15 ± 0.655
5.15ValVal: 5.15 ± 0.862
1.484ValTrp: 1.484 ± 0.364
1.397ValTyr: 1.397 ± 0.299
0.0ValXaa: 0.0 ± 0.0
Trp
0.96TrpAla: 0.96 ± 0.323
0.349TrpCys: 0.349 ± 0.176
0.698TrpAsp: 0.698 ± 0.228
1.571TrpGlu: 1.571 ± 0.545
0.524TrpPhe: 0.524 ± 0.206
0.349TrpGly: 0.349 ± 0.163
0.087TrpHis: 0.087 ± 0.086
0.524TrpIle: 0.524 ± 0.231
0.786TrpLys: 0.786 ± 0.247
1.222TrpLeu: 1.222 ± 0.35
0.698TrpMet: 0.698 ± 0.246
0.873TrpAsn: 0.873 ± 0.318
0.524TrpPro: 0.524 ± 0.241
0.698TrpGln: 0.698 ± 0.253
1.222TrpArg: 1.222 ± 0.303
1.135TrpSer: 1.135 ± 0.335
0.96TrpThr: 0.96 ± 0.259
1.135TrpVal: 1.135 ± 0.35
0.349TrpTrp: 0.349 ± 0.142
0.349TrpTyr: 0.349 ± 0.149
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.833TyrAla: 1.833 ± 0.427
0.262TyrCys: 0.262 ± 0.131
1.658TyrAsp: 1.658 ± 0.314
1.571TyrGlu: 1.571 ± 0.358
0.786TyrPhe: 0.786 ± 0.288
1.746TyrGly: 1.746 ± 0.411
0.524TyrHis: 0.524 ± 0.254
1.047TyrIle: 1.047 ± 0.222
1.135TyrLys: 1.135 ± 0.33
2.182TyrLeu: 2.182 ± 0.386
0.436TyrMet: 0.436 ± 0.225
0.698TyrAsn: 0.698 ± 0.21
1.222TyrPro: 1.222 ± 0.408
0.611TyrGln: 0.611 ± 0.224
2.182TyrArg: 2.182 ± 0.349
1.397TyrSer: 1.397 ± 0.315
1.222TyrThr: 1.222 ± 0.373
1.658TyrVal: 1.658 ± 0.328
0.262TyrTrp: 0.262 ± 0.147
0.349TyrTyr: 0.349 ± 0.164
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 52 proteins (11458 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski