Chlamydomonas eustigma

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae; CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14139 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A250XQE6|A0A250XQE6_9CHLO Isoform of A0A250XQE7 Uncharacterized protein (Fragment) OS=Chlamydomonas eustigma OX=1157962 GN=CEUSTIGMA_g12702.t1 PE=4 SV=1
MM1 pKa = 6.85VQQHH5 pKa = 6.37DD6 pKa = 3.79VGMDD10 pKa = 3.51HH11 pKa = 6.99QPVEE15 pKa = 4.37QQQHH19 pKa = 6.52DD20 pKa = 3.95GGMDD24 pKa = 3.51HH25 pKa = 6.93QPVEE29 pKa = 4.37QQQHH33 pKa = 6.52DD34 pKa = 3.73GGMDD38 pKa = 3.44HH39 pKa = 7.17QSVEE43 pKa = 4.28QQQHH47 pKa = 6.47DD48 pKa = 3.9GGMDD52 pKa = 3.51HH53 pKa = 6.93QPVEE57 pKa = 4.22QQQQEE62 pKa = 4.3GGMDD66 pKa = 3.7HH67 pKa = 6.8QPVEE71 pKa = 4.37QQQHH75 pKa = 6.37DD76 pKa = 4.44GGTEE80 pKa = 3.74TCGPVDD86 pKa = 4.67QPWLQQPTLADD97 pKa = 3.65EE98 pKa = 4.71VGSEE102 pKa = 5.18AEE104 pKa = 3.96IEE106 pKa = 4.32NNGAHH111 pKa = 7.26PDD113 pKa = 3.56GSSAEE118 pKa = 4.23VVGNMVSPSEE128 pKa = 4.06DD129 pKa = 2.83AVIEE133 pKa = 4.21VEE135 pKa = 4.31YY136 pKa = 11.23VPAEE140 pKa = 4.18DD141 pKa = 3.44MHH143 pKa = 8.56AEE145 pKa = 3.97SDD147 pKa = 3.65QAGDD151 pKa = 3.52STEE154 pKa = 4.19GVV156 pKa = 3.42

Molecular weight: 16.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A250XC52|A0A250XC52_9CHLO Isoform of A0A250XCJ4 Uncharacterized protein (Fragment) OS=Chlamydomonas eustigma OX=1157962 GN=CEUSTIGMA_g7918.t1 PE=4 SV=1
MM1 pKa = 7.58RR2 pKa = 11.84LVAMRR7 pKa = 11.84LIVMRR12 pKa = 11.84LIVMTMIVMRR22 pKa = 11.84LVVMRR27 pKa = 11.84LVAMRR32 pKa = 11.84LIVMRR37 pKa = 11.84LIVMRR42 pKa = 11.84LVVMRR47 pKa = 11.84LIAMRR52 pKa = 11.84LIVMTMIVMRR62 pKa = 11.84LVVMRR67 pKa = 11.84LIVMRR72 pKa = 11.84LVAMRR77 pKa = 11.84LIAMRR82 pKa = 11.84LIVMRR87 pKa = 11.84LVVMRR92 pKa = 11.84LVAMRR97 pKa = 11.84LIVMRR102 pKa = 11.84LIVMRR107 pKa = 11.84LVVMRR112 pKa = 11.84LIVMTMIVV120 pKa = 3.14

Molecular weight: 14.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14046

93

14139

8159502

22

13200

577.1

62.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.535 ± 0.026

1.64 ± 0.009

4.948 ± 0.014

5.873 ± 0.023

2.892 ± 0.013

7.075 ± 0.022

2.684 ± 0.012

3.925 ± 0.013

4.532 ± 0.019

9.758 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.64 ± 0.009

3.325 ± 0.011

5.787 ± 0.03

4.832 ± 0.017

5.356 ± 0.015

9.725 ± 0.03

5.444 ± 0.014

6.63 ± 0.013

1.148 ± 0.006

2.249 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski