Amino acid dipepetide frequency for Chlamydomonas eustigma

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	14.503AlaAla: 14.503 ± 0.11	1.584AlaCys: 1.584 ± 0.018	4.143AlaAsp: 4.143 ± 0.03	5.464AlaGlu: 5.464 ± 0.042	2.635AlaPhe: 2.635 ± 0.02	6.972AlaGly: 6.972 ± 0.045	1.95AlaHis: 1.95 ± 0.018	3.293AlaIle: 3.293 ± 0.022	3.52AlaLys: 3.52 ± 0.031	9.203AlaLeu: 9.203 ± 0.05	2.433AlaMet: 2.433 ± 0.019	2.349AlaAsn: 2.349 ± 0.018	4.867AlaPro: 4.867 ± 0.039	3.476AlaGln: 3.476 ± 0.024	4.16AlaArg: 4.16 ± 0.027	9.438AlaSer: 9.438 ± 0.046	5.214AlaThr: 5.214 ± 0.034	7.26AlaVal: 7.26 ± 0.034	1.081AlaTrp: 1.081 ± 0.013	1.801AlaTyr: 1.801 ± 0.016	0.0AlaXaa: 0.0 ± 0.0
Cys	1.095CysAla: 1.095 ± 0.013	0.482CysCys: 0.482 ± 0.01	0.741CysAsp: 0.741 ± 0.01	0.755CysGlu: 0.755 ± 0.01	0.573CysPhe: 0.573 ± 0.009	1.168CysGly: 1.168 ± 0.014	0.458CysHis: 0.458 ± 0.008	0.766CysIle: 0.766 ± 0.01	0.766CysLys: 0.766 ± 0.01	1.65CysLeu: 1.65 ± 0.016	0.477CysMet: 0.477 ± 0.008	0.688CysAsn: 0.688 ± 0.011	0.862CysPro: 0.862 ± 0.012	0.68CysGln: 0.68 ± 0.01	0.907CysArg: 0.907 ± 0.011	1.695CysSer: 1.695 ± 0.019	1.018CysThr: 1.018 ± 0.014	0.971CysVal: 0.971 ± 0.013	0.244CysTrp: 0.244 ± 0.005	0.407CysTyr: 0.407 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	4.382AspAla: 4.382 ± 0.031	0.814AspCys: 0.814 ± 0.012	3.227AspAsp: 3.227 ± 0.041	3.457AspGlu: 3.457 ± 0.025	1.573AspPhe: 1.573 ± 0.013	3.461AspGly: 3.461 ± 0.026	1.23AspHis: 1.23 ± 0.014	2.364AspIle: 2.364 ± 0.018	2.095AspLys: 2.095 ± 0.022	4.89AspLeu: 4.89 ± 0.031	1.483AspMet: 1.483 ± 0.015	1.594AspAsn: 1.594 ± 0.018	2.689AspPro: 2.689 ± 0.022	1.875AspGln: 1.875 ± 0.019	2.372AspArg: 2.372 ± 0.021	3.955AspSer: 3.955 ± 0.027	2.343AspThr: 2.343 ± 0.019	3.877AspVal: 3.877 ± 0.024	0.624AspTrp: 0.624 ± 0.01	1.174AspTyr: 1.174 ± 0.014	0.0AspXaa: 0.0 ± 0.0
Glu	6.451GluAla: 6.451 ± 0.046	0.75GluCys: 0.75 ± 0.01	3.872GluAsp: 3.872 ± 0.03	5.852GluGlu: 5.852 ± 0.056	1.42GluPhe: 1.42 ± 0.016	4.509GluGly: 4.509 ± 0.031	1.373GluHis: 1.373 ± 0.015	2.164GluIle: 2.164 ± 0.018	2.721GluLys: 2.721 ± 0.027	5.551GluLeu: 5.551 ± 0.038	1.457GluMet: 1.457 ± 0.016	1.618GluAsn: 1.618 ± 0.015	2.252GluPro: 2.252 ± 0.022	2.692GluGln: 2.692 ± 0.021	3.399GluArg: 3.399 ± 0.032	3.851GluSer: 3.851 ± 0.026	2.469GluThr: 2.469 ± 0.02	4.458GluVal: 4.458 ± 0.026	0.638GluTrp: 0.638 ± 0.011	1.237GluTyr: 1.237 ± 0.012	0.0GluXaa: 0.0 ± 0.0
Phe	2.018PheAla: 2.018 ± 0.019	0.546PheCys: 0.546 ± 0.009	1.517PheAsp: 1.517 ± 0.014	1.646PheGlu: 1.646 ± 0.018	1.074PhePhe: 1.074 ± 0.015	2.049PheGly: 2.049 ± 0.019	0.728PheHis: 0.728 ± 0.009	1.295PheIle: 1.295 ± 0.015	1.596PheLys: 1.596 ± 0.016	2.792PheLeu: 2.792 ± 0.024	0.83PheMet: 0.83 ± 0.011	1.21PheAsn: 1.21 ± 0.014	1.46PhePro: 1.46 ± 0.016	1.331PheGln: 1.331 ± 0.012	1.43PheArg: 1.43 ± 0.013	2.789PheSer: 2.789 ± 0.021	1.626PheThr: 1.626 ± 0.014	1.83PheVal: 1.83 ± 0.018	0.395PheTrp: 0.395 ± 0.007	0.759PheTyr: 0.759 ± 0.011	0.0PheXaa: 0.0 ± 0.0
Gly	5.958GlyAla: 5.958 ± 0.042	1.251GlyCys: 1.251 ± 0.012	3.327GlyAsp: 3.327 ± 0.025	3.27GlyGlu: 3.27 ± 0.026	2.176GlyPhe: 2.176 ± 0.019	7.095GlyGly: 7.095 ± 0.065	1.96GlyHis: 1.96 ± 0.017	2.935GlyIle: 2.935 ± 0.021	3.035GlyLys: 3.035 ± 0.024	6.532GlyLeu: 6.532 ± 0.028	1.97GlyMet: 1.97 ± 0.021	2.357GlyAsn: 2.357 ± 0.019	3.334GlyPro: 3.334 ± 0.028	2.805GlyGln: 2.805 ± 0.021	4.198GlyArg: 4.198 ± 0.031	7.38GlySer: 7.38 ± 0.042	3.948GlyThr: 3.948 ± 0.029	4.656GlyVal: 4.656 ± 0.03	0.859GlyTrp: 0.859 ± 0.011	1.711GlyTyr: 1.711 ± 0.018	0.0GlyXaa: 0.0 ± 0.0
His	2.301HisAla: 2.301 ± 0.018	0.457HisCys: 0.457 ± 0.008	1.385HisAsp: 1.385 ± 0.015	1.407HisGlu: 1.407 ± 0.016	0.782HisPhe: 0.782 ± 0.012	1.792HisGly: 1.792 ± 0.017	1.224HisHis: 1.224 ± 0.018	1.11HisIle: 1.11 ± 0.013	1.037HisLys: 1.037 ± 0.012	2.627HisLeu: 2.627 ± 0.023	0.681HisMet: 0.681 ± 0.009	1.003HisAsn: 1.003 ± 0.011	1.709HisPro: 1.709 ± 0.018	1.506HisGln: 1.506 ± 0.017	1.362HisArg: 1.362 ± 0.014	2.308HisSer: 2.308 ± 0.023	1.372HisThr: 1.372 ± 0.016	1.844HisVal: 1.844 ± 0.016	0.294HisTrp: 0.294 ± 0.006	0.639HisTyr: 0.639 ± 0.011	0.0HisXaa: 0.0 ± 0.0
Ile	3.234IleAla: 3.234 ± 0.022	0.691IleCys: 0.691 ± 0.01	1.871IleAsp: 1.871 ± 0.018	2.12IleGlu: 2.12 ± 0.019	1.255IlePhe: 1.255 ± 0.014	2.228IleGly: 2.228 ± 0.018	1.031IleHis: 1.031 ± 0.012	1.835IleIle: 1.835 ± 0.017	2.123IleLys: 2.123 ± 0.021	3.759IleLeu: 3.759 ± 0.025	1.22IleMet: 1.22 ± 0.014	1.547IleAsn: 1.547 ± 0.015	2.339IlePro: 2.339 ± 0.02	1.902IleGln: 1.902 ± 0.016	2.11IleArg: 2.11 ± 0.017	3.916IleSer: 3.916 ± 0.023	2.368IleThr: 2.368 ± 0.02	2.439IleVal: 2.439 ± 0.021	0.437IleTrp: 0.437 ± 0.008	0.827IleTyr: 0.827 ± 0.011	0.0IleXaa: 0.0 ± 0.0
Lys	4.414LysAla: 4.414 ± 0.034	0.589LysCys: 0.589 ± 0.009	2.47LysAsp: 2.47 ± 0.022	3.327LysGlu: 3.327 ± 0.027	1.144LysPhe: 1.144 ± 0.014	3.135LysGly: 3.135 ± 0.027	1.117LysHis: 1.117 ± 0.014	1.746LysIle: 1.746 ± 0.015	2.768LysLys: 2.768 ± 0.035	4.381LysLeu: 4.381 ± 0.028	1.175LysMet: 1.175 ± 0.012	1.393LysAsn: 1.393 ± 0.015	2.199LysPro: 2.199 ± 0.017	2.354LysGln: 2.354 ± 0.018	2.792LysArg: 2.792 ± 0.021	3.437LysSer: 3.437 ± 0.023	2.22LysThr: 2.22 ± 0.019	3.087LysVal: 3.087 ± 0.022	0.501LysTrp: 0.501 ± 0.008	1.07LysTyr: 1.07 ± 0.014	0.0LysXaa: 0.0 ± 0.0
Leu	7.909LeuAla: 7.909 ± 0.043	1.542LeuCys: 1.542 ± 0.016	4.833LeuAsp: 4.833 ± 0.026	5.8LeuGlu: 5.8 ± 0.037	2.63LeuPhe: 2.63 ± 0.021	6.096LeuGly: 6.096 ± 0.03	2.849LeuHis: 2.849 ± 0.022	3.627LeuIle: 3.627 ± 0.024	5.027LeuLys: 5.027 ± 0.03	10.602LeuLeu: 10.602 ± 0.057	2.605LeuMet: 2.605 ± 0.02	3.334LeuAsn: 3.334 ± 0.026	6.394LeuPro: 6.394 ± 0.037	5.626LeuGln: 5.626 ± 0.036	5.659LeuArg: 5.659 ± 0.038	9.116LeuSer: 9.116 ± 0.043	5.022LeuThr: 5.022 ± 0.031	5.788LeuVal: 5.788 ± 0.034	1.012LeuTrp: 1.012 ± 0.013	2.113LeuTyr: 2.113 ± 0.02	0.0LeuXaa: 0.0 ± 0.0
Met	2.332MetAla: 2.332 ± 0.019	0.38MetCys: 0.38 ± 0.007	1.397MetAsp: 1.397 ± 0.014	1.641MetGlu: 1.641 ± 0.014	0.735MetPhe: 0.735 ± 0.01	1.603MetGly: 1.603 ± 0.02	0.716MetHis: 0.716 ± 0.01	0.98MetIle: 0.98 ± 0.013	1.286MetLys: 1.286 ± 0.013	2.634MetLeu: 2.634 ± 0.021	0.963MetMet: 0.963 ± 0.015	0.901MetAsn: 0.901 ± 0.012	1.504MetPro: 1.504 ± 0.016	1.402MetGln: 1.402 ± 0.015	1.49MetArg: 1.49 ± 0.015	2.374MetSer: 2.374 ± 0.02	1.511MetThr: 1.511 ± 0.013	1.6MetVal: 1.6 ± 0.015	0.286MetTrp: 0.286 ± 0.005	0.662MetTyr: 0.662 ± 0.01	0.0MetXaa: 0.0 ± 0.0
Asn	2.839AsnAla: 2.839 ± 0.02	0.539AsnCys: 0.539 ± 0.008	1.497AsnAsp: 1.497 ± 0.014	1.682AsnGlu: 1.682 ± 0.015	1.006AsnPhe: 1.006 ± 0.013	2.315AsnGly: 2.315 ± 0.021	0.826AsnHis: 0.826 ± 0.011	1.667AsnIle: 1.667 ± 0.018	1.731AsnLys: 1.731 ± 0.014	3.095AsnLeu: 3.095 ± 0.023	1.022AsnMet: 1.022 ± 0.014	1.526AsnAsn: 1.526 ± 0.018	1.971AsnPro: 1.971 ± 0.019	1.448AsnGln: 1.448 ± 0.013	1.612AsnArg: 1.612 ± 0.015	3.094AsnSer: 3.094 ± 0.025	1.952AsnThr: 1.952 ± 0.019	2.268AsnVal: 2.268 ± 0.018	0.344AsnTrp: 0.344 ± 0.007	0.819AsnTyr: 0.819 ± 0.012	0.0AsnXaa: 0.0 ± 0.0
Pro	5.569ProAla: 5.569 ± 0.041	0.825ProCys: 0.825 ± 0.013	2.607ProAsp: 2.607 ± 0.02	3.186ProGlu: 3.186 ± 0.026	1.646ProPhe: 1.646 ± 0.017	4.047ProGly: 4.047 ± 0.033	1.585ProHis: 1.585 ± 0.017	1.851ProIle: 1.851 ± 0.017	2.027ProLys: 2.027 ± 0.021	5.296ProLeu: 5.296 ± 0.034	1.076ProMet: 1.076 ± 0.011	1.684ProAsn: 1.684 ± 0.017	5.873ProPro: 5.873 ± 0.127	2.506ProGln: 2.506 ± 0.023	2.458ProArg: 2.458 ± 0.018	6.945ProSer: 6.945 ± 0.06	3.194ProThr: 3.194 ± 0.023	3.621ProVal: 3.621 ± 0.028	0.639ProTrp: 0.639 ± 0.01	1.233ProTyr: 1.233 ± 0.014	0.0ProXaa: 0.0 ± 0.0
Gln	4.563GlnAla: 4.563 ± 0.033	0.639GlnCys: 0.639 ± 0.009	2.243GlnAsp: 2.243 ± 0.017	2.907GlnGlu: 2.907 ± 0.023	1.01GlnPhe: 1.01 ± 0.012	3.257GlnGly: 3.257 ± 0.025	1.722GlnHis: 1.722 ± 0.019	1.573GlnIle: 1.573 ± 0.015	1.956GlnLys: 1.956 ± 0.017	5.147GlnLeu: 5.147 ± 0.033	1.089GlnMet: 1.089 ± 0.013	1.424GlnAsn: 1.424 ± 0.014	2.751GlnPro: 2.751 ± 0.026	4.978GlnGln: 4.978 ± 0.075	2.868GlnArg: 2.868 ± 0.024	3.466GlnSer: 3.466 ± 0.024	1.995GlnThr: 1.995 ± 0.017	3.109GlnVal: 3.109 ± 0.025	0.47GlnTrp: 0.47 ± 0.008	1.147GlnTyr: 1.147 ± 0.014	0.0GlnXaa: 0.0 ± 0.0
Arg	4.365ArgAla: 4.365 ± 0.027	0.904ArgCys: 0.904 ± 0.01	2.616ArgAsp: 2.616 ± 0.021	3.122ArgGlu: 3.122 ± 0.029	1.542ArgPhe: 1.542 ± 0.014	3.714ArgGly: 3.714 ± 0.027	1.558ArgHis: 1.558 ± 0.015	2.141ArgIle: 2.141 ± 0.019	2.671ArgLys: 2.671 ± 0.023	5.395ArgLeu: 5.395 ± 0.035	1.465ArgMet: 1.465 ± 0.015	1.811ArgAsn: 1.811 ± 0.016	3.028ArgPro: 3.028 ± 0.024	2.684ArgGln: 2.684 ± 0.022	3.837ArgArg: 3.837 ± 0.033	4.894ArgSer: 4.894 ± 0.029	2.603ArgThr: 2.603 ± 0.019	3.317ArgVal: 3.317 ± 0.025	0.641ArgTrp: 0.641 ± 0.009	1.248ArgTyr: 1.248 ± 0.012	0.0ArgXaa: 0.0 ± 0.0
Ser	8.766SerAla: 8.766 ± 0.046	1.69SerCys: 1.69 ± 0.018	4.013SerAsp: 4.013 ± 0.024	4.495SerGlu: 4.495 ± 0.029	2.857SerPhe: 2.857 ± 0.021	7.052SerGly: 7.052 ± 0.042	2.485SerHis: 2.485 ± 0.021	3.644SerIle: 3.644 ± 0.024	4.181SerLys: 4.181 ± 0.028	8.658SerLeu: 8.658 ± 0.044	2.338SerMet: 2.338 ± 0.018	3.558SerAsn: 3.558 ± 0.027	5.736SerPro: 5.736 ± 0.065	4.019SerGln: 4.019 ± 0.028	5.13SerArg: 5.13 ± 0.033	13.53SerSer: 13.53 ± 0.086	6.357SerThr: 6.357 ± 0.036	5.653SerVal: 5.653 ± 0.027	1.076SerTrp: 1.076 ± 0.014	2.012SerTyr: 2.012 ± 0.019	0.0SerXaa: 0.0 ± 0.0
Thr	6.069ThrAla: 6.069 ± 0.039	0.986ThrCys: 0.986 ± 0.013	2.313ThrAsp: 2.313 ± 0.021	2.647ThrGlu: 2.647 ± 0.02	1.758ThrPhe: 1.758 ± 0.015	3.804ThrGly: 3.804 ± 0.025	1.263ThrHis: 1.263 ± 0.015	2.029ThrIle: 2.029 ± 0.017	2.062ThrLys: 2.062 ± 0.018	5.22ThrLeu: 5.22 ± 0.031	1.287ThrMet: 1.287 ± 0.016	1.662ThrAsn: 1.662 ± 0.017	3.279ThrPro: 3.279 ± 0.026	2.127ThrGln: 2.127 ± 0.017	2.419ThrArg: 2.419 ± 0.017	6.421ThrSer: 6.421 ± 0.036	3.682ThrThr: 3.682 ± 0.033	3.538ThrVal: 3.538 ± 0.02	0.667ThrTrp: 0.667 ± 0.01	1.207ThrTyr: 1.207 ± 0.014	0.0ThrXaa: 0.0 ± 0.0
Val	6.117ValAla: 6.117 ± 0.036	1.024ValCys: 1.024 ± 0.013	3.561ValAsp: 3.561 ± 0.024	4.065ValGlu: 4.065 ± 0.03	1.944ValPhe: 1.944 ± 0.018	4.103ValGly: 4.103 ± 0.028	1.837ValHis: 1.837 ± 0.014	2.724ValIle: 2.724 ± 0.02	3.107ValLys: 3.107 ± 0.023	6.789ValLeu: 6.789 ± 0.038	1.857ValMet: 1.857 ± 0.018	2.132ValAsn: 2.132 ± 0.022	3.93ValPro: 3.93 ± 0.026	3.245ValGln: 3.245 ± 0.023	3.311ValArg: 3.311 ± 0.023	5.655ValSer: 5.655 ± 0.031	3.764ValThr: 3.764 ± 0.026	4.925ValVal: 4.925 ± 0.033	0.775ValTrp: 0.775 ± 0.012	1.443ValTyr: 1.443 ± 0.014	0.0ValXaa: 0.0 ± 0.0
Trp	0.846TrpAla: 0.846 ± 0.012	0.223TrpCys: 0.223 ± 0.006	0.64TrpAsp: 0.64 ± 0.011	0.656TrpGlu: 0.656 ± 0.009	0.349TrpPhe: 0.349 ± 0.007	0.75TrpGly: 0.75 ± 0.012	0.292TrpHis: 0.292 ± 0.007	0.484TrpIle: 0.484 ± 0.009	0.569TrpLys: 0.569 ± 0.008	1.164TrpLeu: 1.164 ± 0.012	0.342TrpMet: 0.342 ± 0.007	0.418TrpAsn: 0.418 ± 0.009	0.537TrpPro: 0.537 ± 0.01	0.593TrpGln: 0.593 ± 0.009	0.82TrpArg: 0.82 ± 0.011	1.022TrpSer: 1.022 ± 0.015	0.611TrpThr: 0.611 ± 0.009	0.669TrpVal: 0.669 ± 0.011	0.208TrpTrp: 0.208 ± 0.006	0.292TrpTyr: 0.292 ± 0.006	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.616TyrAla: 1.616 ± 0.016	0.488TyrCys: 0.488 ± 0.009	1.207TyrAsp: 1.207 ± 0.015	1.236TyrGlu: 1.236 ± 0.012	0.812TyrPhe: 0.812 ± 0.011	1.604TyrGly: 1.604 ± 0.019	0.637TyrHis: 0.637 ± 0.009	1.025TyrIle: 1.025 ± 0.013	1.04TyrLys: 1.04 ± 0.013	2.197TyrLeu: 2.197 ± 0.016	0.631TyrMet: 0.631 ± 0.01	1.044TyrAsn: 1.044 ± 0.015	1.156TyrPro: 1.156 ± 0.016	1.066TyrGln: 1.066 ± 0.011	1.223TyrArg: 1.223 ± 0.014	1.964TyrSer: 1.964 ± 0.017	1.17TyrThr: 1.17 ± 0.012	1.392TyrVal: 1.392 ± 0.015	0.292TyrTrp: 0.292 ± 0.006	0.693TyrTyr: 0.693 ± 0.013	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 14139 proteins (8159502 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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