Corynebacterium phage Cruella

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Ceetrepovirus; unclassified Ceetrepovirus

Average proteome isoelectric point is 5.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 92 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G3LX90|A0A3G3LX90_9CAUD Uncharacterized protein OS=Corynebacterium phage Cruella OX=2483718 GN=3 PE=4 SV=1
MM1 pKa = 8.02PDD3 pKa = 3.09FDD5 pKa = 5.34SFHH8 pKa = 6.6TEE10 pKa = 3.28NDD12 pKa = 3.59VVDD15 pKa = 4.03TSTNLAYY22 pKa = 10.55LDD24 pKa = 3.75YY25 pKa = 11.23LNRR28 pKa = 4.36

Molecular weight: 3.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G3LW64|A0A3G3LW64_9CAUD Lysin A OS=Corynebacterium phage Cruella OX=2483718 GN=36 PE=4 SV=1
MM1 pKa = 7.61GKK3 pKa = 9.83LVWPNYY9 pKa = 9.37GPPRR13 pKa = 11.84YY14 pKa = 10.0DD15 pKa = 3.92IINKK19 pKa = 9.82GYY21 pKa = 10.71LKK23 pKa = 10.75ARR25 pKa = 11.84LEE27 pKa = 4.27AQSSHH32 pKa = 5.11KK33 pKa = 10.38HH34 pKa = 5.15PVYY37 pKa = 10.76SSDD40 pKa = 2.46WFSFQEE46 pKa = 4.22SVLNNNPYY54 pKa = 9.84VEE56 pKa = 4.11RR57 pKa = 11.84ALFKK61 pKa = 10.53LAQKK65 pKa = 10.74AVFALKK71 pKa = 10.46RR72 pKa = 11.84EE73 pKa = 4.05IPIGDD78 pKa = 3.63NSKK81 pKa = 10.96DD82 pKa = 2.97GHH84 pKa = 6.61LRR86 pKa = 11.84NKK88 pKa = 8.91VKK90 pKa = 10.5AYY92 pKa = 9.71RR93 pKa = 11.84VRR95 pKa = 11.84KK96 pKa = 9.92GGLAKK101 pKa = 10.57DD102 pKa = 3.29RR103 pKa = 11.84TVYY106 pKa = 9.65EE107 pKa = 4.13VHH109 pKa = 6.98AGGHH113 pKa = 4.57TKK115 pKa = 9.9EE116 pKa = 5.51DD117 pKa = 3.33RR118 pKa = 11.84SKK120 pKa = 9.85WHH122 pKa = 6.12AALYY126 pKa = 10.1RR127 pKa = 11.84STYY130 pKa = 10.89SSAEE134 pKa = 3.86DD135 pKa = 3.45YY136 pKa = 11.15KK137 pKa = 11.22NRR139 pKa = 11.84KK140 pKa = 7.4LTEE143 pKa = 3.97TGDD146 pKa = 3.7RR147 pKa = 11.84IGWLRR152 pKa = 11.84SAEE155 pKa = 3.95RR156 pKa = 11.84RR157 pKa = 11.84LSDD160 pKa = 3.23GG161 pKa = 3.62

Molecular weight: 18.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

92

0

92

20226

23

2143

219.8

24.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.983 ± 0.541

0.707 ± 0.115

6.838 ± 0.244

7.792 ± 0.345

3.802 ± 0.213

7.5 ± 0.456

1.775 ± 0.159

5.691 ± 0.153

5.068 ± 0.197

7.841 ± 0.256

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.615 ± 0.143

4.282 ± 0.198

4.381 ± 0.142

3.065 ± 0.116

5.854 ± 0.263

6.462 ± 0.23

5.646 ± 0.272

6.66 ± 0.176

1.646 ± 0.125

3.392 ± 0.225
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski