Natrarchaeobius chitinivorans
Average proteome isoelectric point is 4.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4237 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3N6LTQ8|A0A3N6LTQ8_9EURY Uncharacterized protein OS=Natrarchaeobius chitinivorans OX=1679083 GN=EA473_19830 PE=4 SV=1
MM1 pKa = 7.4 KK2 pKa = 10.49 RR3 pKa = 11.84 LLLVVLVAAMLALAGCADD21 pKa = 3.85 GTGTGTDD28 pKa = 4.43 DD29 pKa = 3.68 PTVDD33 pKa = 4.31 ADD35 pKa = 5.05 DD36 pKa = 5.25 DD37 pKa = 4.67 LEE39 pKa = 4.37 EE40 pKa = 4.49 TDD42 pKa = 5.63 PDD44 pKa = 3.76 ATDD47 pKa = 4.89 DD48 pKa = 4.93 DD49 pKa = 5.18 SEE51 pKa = 4.3 TDD53 pKa = 3.38 EE54 pKa = 5.19 AKK56 pKa = 10.39 PDD58 pKa = 3.67 SDD60 pKa = 4.04 EE61 pKa = 5.58 SDD63 pKa = 3.59 DD64 pKa = 5.8 AEE66 pKa = 4.73 TNDD69 pKa = 4.51 SDD71 pKa = 5.77 SGDD74 pKa = 3.88 DD75 pKa = 3.76 PNIDD79 pKa = 3.51 GEE81 pKa = 4.61 LEE83 pKa = 3.71 IHH85 pKa = 6.92 HH86 pKa = 7.0 IDD88 pKa = 4.14 VGQADD93 pKa = 4.2 ATLLIEE99 pKa = 4.42 PSGEE103 pKa = 4.02 TMLIDD108 pKa = 4.48 SGDD111 pKa = 3.24 WRR113 pKa = 11.84 QGGSEE118 pKa = 4.16 VIAYY122 pKa = 10.44 LEE124 pKa = 4.22 DD125 pKa = 3.63 QNVNRR130 pKa = 11.84 IDD132 pKa = 3.83 HH133 pKa = 6.55 LVATHH138 pKa = 5.68 GHH140 pKa = 6.58 ADD142 pKa = 3.61 HH143 pKa = 7.2 IGGHH147 pKa = 5.85 DD148 pKa = 4.36 AIIEE152 pKa = 4.24 HH153 pKa = 6.69 YY154 pKa = 7.84 EE155 pKa = 3.93 TEE157 pKa = 4.24 RR158 pKa = 11.84 NGIGAAYY165 pKa = 10.03 DD166 pKa = 3.25 SGVAHH171 pKa = 7.04 TSQTYY176 pKa = 9.15 EE177 pKa = 4.16 RR178 pKa = 11.84 YY179 pKa = 10.07 LDD181 pKa = 5.47 AIDD184 pKa = 3.6 EE185 pKa = 4.75 HH186 pKa = 8.06 NVEE189 pKa = 4.21 LLIVEE194 pKa = 4.51 EE195 pKa = 4.54 GDD197 pKa = 3.59 HH198 pKa = 7.91 IEE200 pKa = 4.81 FGDD203 pKa = 4.2 ANVDD207 pKa = 3.62 VLNPPTGDD215 pKa = 3.34 SGSDD219 pKa = 2.82 LHH221 pKa = 6.87 YY222 pKa = 11.25 NSVTLAIEE230 pKa = 4.25 FGEE233 pKa = 4.15 FSYY236 pKa = 9.84 LTTGDD241 pKa = 3.51 VEE243 pKa = 5.0 ADD245 pKa = 2.97 AEE247 pKa = 4.03 QRR249 pKa = 11.84 MVDD252 pKa = 3.4 EE253 pKa = 5.63 HH254 pKa = 7.45 DD255 pKa = 4.86 DD256 pKa = 3.77 SLEE259 pKa = 4.2 ADD261 pKa = 4.32 AYY263 pKa = 10.05 QAGHH267 pKa = 6.74 HH268 pKa = 6.31 GSSTSSTTPFMNEE281 pKa = 3.45 VTPAIAIISSAYY293 pKa = 9.72 DD294 pKa = 3.16 SQYY297 pKa = 11.13 GHH299 pKa = 6.95 PHH301 pKa = 6.9 DD302 pKa = 5.27 EE303 pKa = 4.19 VLEE306 pKa = 4.76 DD307 pKa = 3.61 YY308 pKa = 10.99 ADD310 pKa = 4.12 RR311 pKa = 11.84 GIEE314 pKa = 4.25 TYY316 pKa = 7.55 WTAVHH321 pKa = 6.6 GDD323 pKa = 3.59 VVLTTDD329 pKa = 3.27 GTDD332 pKa = 3.07 VEE334 pKa = 5.53 LEE336 pKa = 4.34 TEE338 pKa = 4.47 HH339 pKa = 6.78 EE340 pKa = 4.6 FSTEE344 pKa = 3.69 AADD347 pKa = 4.83 LLEE350 pKa = 5.01 EE351 pKa = 4.98 KK352 pKa = 10.29 PADD355 pKa = 4.52 DD356 pKa = 4.27 DD357 pKa = 4.0 TQASVTHH364 pKa = 7.05 LIDD367 pKa = 3.66 VAPAPLDD374 pKa = 3.49 GG375 pKa = 4.96
Molecular weight: 40.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.688
IPC2_protein 3.681
IPC_protein 3.719
Toseland 3.49
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.643
Rodwell 3.541
Grimsley 3.401
Solomon 3.706
Lehninger 3.668
Nozaki 3.821
DTASelect 4.062
Thurlkill 3.541
EMBOSS 3.656
Sillero 3.846
Patrickios 1.176
IPC_peptide 3.706
IPC2_peptide 3.821
IPC2.peptide.svr19 3.755
Protein with the highest isoelectric point:
>tr|A0A3N6LYG0|A0A3N6LYG0_9EURY Transcription initiation factor IIB OS=Natrarchaeobius chitinivorans OX=1679083 GN=tfb PE=3 SV=1
MM1 pKa = 7.15 VLKK4 pKa = 10.64 KK5 pKa = 10.76 LLGSRR10 pKa = 11.84 ATRR13 pKa = 11.84 SLTVFSVLAEE23 pKa = 4.12 AKK25 pKa = 9.9 RR26 pKa = 11.84 AFGRR30 pKa = 11.84 GRR32 pKa = 11.84 RR33 pKa = 11.84 TRR35 pKa = 11.84 GLLLLGVAALAWKK48 pKa = 7.3 WTVIGLVAQGIVGVLRR64 pKa = 11.84 RR65 pKa = 11.84 GRR67 pKa = 11.84 SGGSSPSS74 pKa = 3.16
Molecular weight: 7.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.399
IPC2_protein 10.847
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.281
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 12.018
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.099
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4237
0
4237
1218109
31
3074
287.5
31.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.848 ± 0.048
0.765 ± 0.013
8.635 ± 0.055
9.199 ± 0.059
3.316 ± 0.029
8.262 ± 0.043
2.038 ± 0.018
4.568 ± 0.032
1.749 ± 0.023
8.747 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.702 ± 0.015
2.357 ± 0.021
4.646 ± 0.025
2.372 ± 0.023
6.683 ± 0.044
5.856 ± 0.028
6.479 ± 0.033
8.907 ± 0.038
1.133 ± 0.014
2.738 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here