Tessaracoccus flavus
Average proteome isoelectric point is 5.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2784 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Q2CGY6|A0A1Q2CGY6_9ACTN Predicted ATP-dependent carboligase ATP-grasp superfamily OS=Tessaracoccus flavus OX=1610493 GN=RPIT_11610 PE=4 SV=1
MM1 pKa = 7.22 ITFKK5 pKa = 10.97 KK6 pKa = 10.59 ALTATALASALLLTACGGDD25 pKa = 3.76 GDD27 pKa = 5.22 DD28 pKa = 4.67 PAPGATDD35 pKa = 3.41 PAMTTPAATSGEE47 pKa = 4.47 TTGGEE52 pKa = 4.03 TTGAGEE58 pKa = 4.29 APAASGTINFFTDD71 pKa = 2.93 KK72 pKa = 10.92 AAWEE76 pKa = 4.11 PSFEE80 pKa = 4.21 EE81 pKa = 4.11 MNAASGDD88 pKa = 3.27 IDD90 pKa = 5.44 LEE92 pKa = 4.18 FTGYY96 pKa = 10.11 SDD98 pKa = 3.4 PVAYY102 pKa = 8.23 DD103 pKa = 3.02 TYY105 pKa = 10.73 IRR107 pKa = 11.84 QAFRR111 pKa = 11.84 TDD113 pKa = 3.51 DD114 pKa = 3.86 KK115 pKa = 11.38 PDD117 pKa = 3.27 LFTWHH122 pKa = 6.9 TGGKK126 pKa = 8.59 LQEE129 pKa = 4.31 LAEE132 pKa = 4.15 QGLVAEE138 pKa = 4.98 TTDD141 pKa = 2.62 IWAEE145 pKa = 3.97 AEE147 pKa = 4.31 SEE149 pKa = 4.31 GLVPEE154 pKa = 4.41 GLKK157 pKa = 10.55 DD158 pKa = 3.18 QYY160 pKa = 11.38 TYY162 pKa = 11.08 DD163 pKa = 3.43 GKK165 pKa = 10.78 QYY167 pKa = 10.64 CVPLNVAYY175 pKa = 9.29 WAVYY179 pKa = 9.01 YY180 pKa = 10.53 NKK182 pKa = 10.22 QIFDD186 pKa = 4.64 DD187 pKa = 4.42 NGLTPPTTWEE197 pKa = 3.77 EE198 pKa = 4.43 MEE200 pKa = 4.55 TVMQTLRR207 pKa = 11.84 DD208 pKa = 3.54 AGVTPFHH215 pKa = 6.69 QMNIIFEE222 pKa = 4.2 FVYY225 pKa = 9.92 FQALLAGQDD234 pKa = 3.34 PDD236 pKa = 3.82 TYY238 pKa = 11.26 LGILDD243 pKa = 4.58 GSASYY248 pKa = 10.69 TDD250 pKa = 3.48 EE251 pKa = 4.48 DD252 pKa = 4.51 VNVAVDD258 pKa = 3.11 KK259 pKa = 10.26 WEE261 pKa = 3.83 EE262 pKa = 3.91 MINRR266 pKa = 11.84 GDD268 pKa = 4.03 FIDD271 pKa = 4.47 PGVTGDD277 pKa = 3.86 PQALLMNGDD286 pKa = 2.8 VAMAYY291 pKa = 10.11 FGTFFTGQLTAIDD304 pKa = 3.95 AVSGEE309 pKa = 4.41 DD310 pKa = 3.18 YY311 pKa = 11.14 GIFMFPNLNPDD322 pKa = 3.46 TEE324 pKa = 4.4 EE325 pKa = 3.98 QPMILEE331 pKa = 4.51 TGPLCVGAGSEE342 pKa = 4.5 NEE344 pKa = 4.16 DD345 pKa = 3.33 TALAYY350 pKa = 10.26 SKK352 pKa = 9.62 WWLTTDD358 pKa = 3.9 AQTAWSQSRR367 pKa = 11.84 GDD369 pKa = 3.36 VSFNPNVEE377 pKa = 3.96 IEE379 pKa = 4.21 DD380 pKa = 4.39 PEE382 pKa = 4.69 LSALVEE388 pKa = 4.07 AVNDD392 pKa = 3.88 GTRR395 pKa = 11.84 ILPRR399 pKa = 11.84 YY400 pKa = 9.88 LEE402 pKa = 4.73 GSPQPVYY409 pKa = 10.6 QLSTEE414 pKa = 4.05 LFGAFVTSPSDD425 pKa = 3.26 PRR427 pKa = 11.84 GMQEE431 pKa = 4.13 RR432 pKa = 11.84 LQAAADD438 pKa = 4.48 DD439 pKa = 4.27 YY440 pKa = 10.85 WAQQQ444 pKa = 3.0
Molecular weight: 48.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.703
IPC2_protein 3.681
IPC_protein 3.694
Toseland 3.478
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.592
Rodwell 3.516
Grimsley 3.389
Solomon 3.668
Lehninger 3.63
Nozaki 3.783
DTASelect 4.012
Thurlkill 3.528
EMBOSS 3.605
Sillero 3.808
Patrickios 1.303
IPC_peptide 3.668
IPC2_peptide 3.795
IPC2.peptide.svr19 3.735
Protein with the highest isoelectric point:
>tr|A0A1Q2CC96|A0A1Q2CC96_9ACTN Uncharacterized protein OS=Tessaracoccus flavus OX=1610493 GN=RPIT_02000 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.99 KK16 pKa = 9.77 HH17 pKa = 5.81 RR18 pKa = 11.84 KK19 pKa = 8.55 LLKK22 pKa = 8.44 KK23 pKa = 9.1 TRR25 pKa = 11.84 IQRR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 AGKK33 pKa = 9.79
Molecular weight: 4.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2784
0
2784
923675
33
12360
331.8
35.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.391 ± 0.065
0.675 ± 0.016
6.359 ± 0.041
5.908 ± 0.05
3.082 ± 0.031
8.849 ± 0.065
2.116 ± 0.03
4.291 ± 0.037
2.223 ± 0.032
10.227 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.043 ± 0.027
2.149 ± 0.027
5.421 ± 0.038
2.979 ± 0.023
7.141 ± 0.074
5.609 ± 0.034
6.079 ± 0.076
8.818 ± 0.043
1.568 ± 0.022
2.073 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here