Desulfovibrio mexicanus
Average proteome isoelectric point is 6.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3215 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A239CIS2|A0A239CIS2_9DELT Bifunctional protein GlmU OS=Desulfovibrio mexicanus OX=147047 GN=glmU PE=3 SV=1
MM1 pKa = 7.96 DD2 pKa = 6.28 GILVCIALVIAIVSLRR18 pKa = 11.84 RR19 pKa = 11.84 HH20 pKa = 5.24 GHH22 pKa = 4.2 QEE24 pKa = 3.8 EE25 pKa = 4.52 PSACVAVHH33 pKa = 6.17 KK34 pKa = 11.27 VSDD37 pKa = 3.55 TDD39 pKa = 3.89 GASGASNSSLFDD51 pKa = 4.17 DD52 pKa = 4.63 EE53 pKa = 4.84 WPASSMQSSSFDD65 pKa = 3.6 TDD67 pKa = 2.07 WWTNPAYY74 pKa = 10.57 AFMAGNIYY82 pKa = 10.48 HH83 pKa = 6.0 NTFMDD88 pKa = 3.57 PTYY91 pKa = 10.02 TDD93 pKa = 2.93 WSSTSSSDD101 pKa = 3.93 DD102 pKa = 3.44 SCSSSFGDD110 pKa = 3.94 SLSNSDD116 pKa = 4.33 DD117 pKa = 3.46 WSSSTASFDD126 pKa = 4.11 DD127 pKa = 3.85 SLSSSSCDD135 pKa = 3.68 DD136 pKa = 3.22 SWSSGSFSDD145 pKa = 4.29 SFSSSSFNDD154 pKa = 3.1
Molecular weight: 16.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.781
IPC2_protein 3.554
IPC_protein 3.579
Toseland 3.338
ProMoST 3.745
Dawson 3.605
Bjellqvist 3.808
Wikipedia 3.605
Rodwell 3.401
Grimsley 3.249
Solomon 3.592
Lehninger 3.541
Nozaki 3.732
DTASelect 4.05
Thurlkill 3.427
EMBOSS 3.605
Sillero 3.706
Patrickios 0.426
IPC_peptide 3.579
IPC2_peptide 3.681
IPC2.peptide.svr19 3.717
Protein with the highest isoelectric point:
>tr|A0A239BHP4|A0A239BHP4_9DELT SpoIIIJ-associated protein OS=Desulfovibrio mexicanus OX=147047 GN=SAMN04488503_2556 PE=4 SV=1
MM1 pKa = 7.74 SKK3 pKa = 8.95 RR4 pKa = 11.84 TYY6 pKa = 9.91 QPSKK10 pKa = 9.83 IRR12 pKa = 11.84 RR13 pKa = 11.84 KK14 pKa = 8.4 RR15 pKa = 11.84 TLGFLVRR22 pKa = 11.84 SRR24 pKa = 11.84 TKK26 pKa = 10.51 NGQAVIRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 QKK38 pKa = 10.09 GRR40 pKa = 11.84 KK41 pKa = 8.85 RR42 pKa = 11.84 LALL45 pKa = 3.85
Molecular weight: 5.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.449
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.135
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.082
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3215
0
3215
1069092
37
2824
332.5
36.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.291 ± 0.07
1.416 ± 0.019
5.243 ± 0.034
6.139 ± 0.042
3.665 ± 0.028
8.67 ± 0.046
2.058 ± 0.02
4.088 ± 0.04
3.825 ± 0.04
11.317 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.503 ± 0.021
2.433 ± 0.026
5.335 ± 0.039
3.324 ± 0.027
7.205 ± 0.047
5.22 ± 0.034
4.675 ± 0.029
7.193 ± 0.037
1.194 ± 0.016
2.207 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here