Streptomyces sp. 57

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7533 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A498AY68|A0A498AY68_9ACTN HxlR family transcriptional regulator OS=Streptomyces sp. 57 OX=2035243 GN=CLZ79_7219 PE=4 SV=1
MM1 pKa = 7.66SDD3 pKa = 4.13DD4 pKa = 3.9VLSVIPTDD12 pKa = 3.58PHH14 pKa = 5.26WQPEE18 pKa = 3.82QAAADD23 pKa = 4.04RR24 pKa = 11.84AASIVEE30 pKa = 3.96GLAPGLPDD38 pKa = 4.51GVDD41 pKa = 3.19AEE43 pKa = 5.15IDD45 pKa = 4.29VTWHH49 pKa = 7.07DD50 pKa = 3.82ALTVVDD56 pKa = 5.57CGDD59 pKa = 3.57NLQKK63 pKa = 10.3IGCPHH68 pKa = 7.07CGASIDD74 pKa = 4.15TEE76 pKa = 3.7WWADD80 pKa = 3.52LLEE83 pKa = 4.16AHH85 pKa = 7.01GDD87 pKa = 3.67DD88 pKa = 5.34GFTTLAAVVPCCGAATSLDD107 pKa = 3.5ALDD110 pKa = 4.68YY111 pKa = 10.93DD112 pKa = 4.43WPCGFARR119 pKa = 11.84FEE121 pKa = 3.94IAVWNPEE128 pKa = 3.87RR129 pKa = 11.84DD130 pKa = 3.3WFSDD134 pKa = 3.64EE135 pKa = 3.92EE136 pKa = 4.1LTAIGEE142 pKa = 4.34ALGHH146 pKa = 5.45QVQQVRR152 pKa = 11.84ARR154 pKa = 11.84II155 pKa = 3.61

Molecular weight: 16.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A498BJN6|A0A498BJN6_9ACTN Diaminopimelate decarboxylase OS=Streptomyces sp. 57 OX=2035243 GN=CLZ79_0487 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.51GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7533

0

7533

2468910

29

6674

327.7

35.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.703 ± 0.042

0.796 ± 0.007

6.067 ± 0.025

5.618 ± 0.027

2.69 ± 0.017

9.595 ± 0.029

2.346 ± 0.015

3.002 ± 0.021

2.099 ± 0.024

10.223 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.698 ± 0.012

1.723 ± 0.015

6.198 ± 0.031

2.705 ± 0.014

8.233 ± 0.038

5.122 ± 0.022

6.127 ± 0.02

8.466 ± 0.026

1.523 ± 0.014

2.065 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski