Amino acid dipepetide frequency for Streptomyces sp. 57

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.747AlaAla: 21.747 ± 0.145	1.111AlaCys: 1.111 ± 0.023	8.512AlaAsp: 8.512 ± 0.068	8.748AlaGlu: 8.748 ± 0.082	3.532AlaPhe: 3.532 ± 0.043	13.486AlaGly: 13.486 ± 0.078	2.957AlaHis: 2.957 ± 0.044	3.183AlaIle: 3.183 ± 0.042	2.735AlaLys: 2.735 ± 0.052	14.355AlaLeu: 14.355 ± 0.116	2.389AlaMet: 2.389 ± 0.032	1.837AlaAsn: 1.837 ± 0.028	7.537AlaPro: 7.537 ± 0.082	3.724AlaGln: 3.724 ± 0.043	10.715AlaArg: 10.715 ± 0.074	6.138AlaSer: 6.138 ± 0.059	6.987AlaThr: 6.987 ± 0.06	12.534AlaVal: 12.534 ± 0.084	1.976AlaTrp: 1.976 ± 0.031	2.83AlaTyr: 2.83 ± 0.036	0.0AlaXaa: 0.0 ± 0.0
Cys	1.088CysAla: 1.088 ± 0.022	0.093CysCys: 0.093 ± 0.007	0.478CysAsp: 0.478 ± 0.014	0.387CysGlu: 0.387 ± 0.015	0.209CysPhe: 0.209 ± 0.009	0.988CysGly: 0.988 ± 0.019	0.204CysHis: 0.204 ± 0.009	0.171CysIle: 0.171 ± 0.009	0.125CysLys: 0.125 ± 0.007	0.759CysLeu: 0.759 ± 0.019	0.118CysMet: 0.118 ± 0.006	0.135CysAsn: 0.135 ± 0.008	0.487CysPro: 0.487 ± 0.014	0.155CysGln: 0.155 ± 0.008	0.614CysArg: 0.614 ± 0.016	0.45CysSer: 0.45 ± 0.013	0.547CysThr: 0.547 ± 0.015	0.667CysVal: 0.667 ± 0.016	0.134CysTrp: 0.134 ± 0.008	0.153CysTyr: 0.153 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	7.996AspAla: 7.996 ± 0.069	0.44AspCys: 0.44 ± 0.014	3.661AspAsp: 3.661 ± 0.048	3.942AspGlu: 3.942 ± 0.046	1.666AspPhe: 1.666 ± 0.025	6.652AspGly: 6.652 ± 0.067	1.415AspHis: 1.415 ± 0.024	1.851AspIle: 1.851 ± 0.025	1.171AspLys: 1.171 ± 0.026	6.257AspLeu: 6.257 ± 0.061	0.807AspMet: 0.807 ± 0.021	0.929AspAsn: 0.929 ± 0.021	4.524AspPro: 4.524 ± 0.047	1.472AspGln: 1.472 ± 0.026	5.063AspArg: 5.063 ± 0.056	2.578AspSer: 2.578 ± 0.032	3.291AspThr: 3.291 ± 0.04	4.812AspVal: 4.812 ± 0.047	1.046AspTrp: 1.046 ± 0.02	1.098AspTyr: 1.098 ± 0.023	0.0AspXaa: 0.0 ± 0.0
Glu	7.339GluAla: 7.339 ± 0.067	0.375GluCys: 0.375 ± 0.012	2.941GluAsp: 2.941 ± 0.038	3.534GluGlu: 3.534 ± 0.051	1.403GluPhe: 1.403 ± 0.026	4.34GluGly: 4.34 ± 0.049	1.526GluHis: 1.526 ± 0.025	2.16GluIle: 2.16 ± 0.032	1.434GluLys: 1.434 ± 0.032	6.549GluLeu: 6.549 ± 0.054	0.86GluMet: 0.86 ± 0.022	1.033GluAsn: 1.033 ± 0.022	3.399GluPro: 3.399 ± 0.05	2.237GluGln: 2.237 ± 0.031	5.442GluArg: 5.442 ± 0.062	2.612GluSer: 2.612 ± 0.035	2.877GluThr: 2.877 ± 0.036	4.322GluVal: 4.322 ± 0.045	0.725GluTrp: 0.725 ± 0.017	1.071GluTyr: 1.071 ± 0.023	0.0GluXaa: 0.0 ± 0.0
Phe	3.676PheAla: 3.676 ± 0.038	0.277PheCys: 0.277 ± 0.011	1.94PheAsp: 1.94 ± 0.03	1.416PheGlu: 1.416 ± 0.027	0.858PhePhe: 0.858 ± 0.021	2.985PheGly: 2.985 ± 0.039	0.606PheHis: 0.606 ± 0.016	0.648PheIle: 0.648 ± 0.017	0.505PheLys: 0.505 ± 0.014	2.59PheLeu: 2.59 ± 0.038	0.396PheMet: 0.396 ± 0.012	0.532PheAsn: 0.532 ± 0.015	1.377PhePro: 1.377 ± 0.027	0.632PheGln: 0.632 ± 0.016	1.832PheArg: 1.832 ± 0.03	1.434PheSer: 1.434 ± 0.024	1.994PheThr: 1.994 ± 0.029	2.24PheVal: 2.24 ± 0.031	0.399PheTrp: 0.399 ± 0.013	0.567PheTyr: 0.567 ± 0.016	0.0PheXaa: 0.0 ± 0.0
Gly	11.516GlyAla: 11.516 ± 0.091	0.802GlyCys: 0.802 ± 0.018	5.485GlyAsp: 5.485 ± 0.055	5.041GlyGlu: 5.041 ± 0.052	2.872GlyPhe: 2.872 ± 0.034	9.007GlyGly: 9.007 ± 0.078	2.382GlyHis: 2.382 ± 0.034	3.358GlyIle: 3.358 ± 0.042	2.304GlyLys: 2.304 ± 0.038	9.376GlyLeu: 9.376 ± 0.076	1.965GlyMet: 1.965 ± 0.029	1.751GlyAsn: 1.751 ± 0.035	5.497GlyPro: 5.497 ± 0.053	2.583GlyGln: 2.583 ± 0.031	8.307GlyArg: 8.307 ± 0.062	5.617GlySer: 5.617 ± 0.057	6.642GlyThr: 6.642 ± 0.059	7.506GlyVal: 7.506 ± 0.062	1.695GlyTrp: 1.695 ± 0.027	2.247GlyTyr: 2.247 ± 0.034	0.0GlyXaa: 0.0 ± 0.0
His	2.771HisAla: 2.771 ± 0.035	0.213HisCys: 0.213 ± 0.009	1.384HisAsp: 1.384 ± 0.026	1.256HisGlu: 1.256 ± 0.022	0.649HisPhe: 0.649 ± 0.019	2.532HisGly: 2.532 ± 0.033	0.725HisHis: 0.725 ± 0.022	0.668HisIle: 0.668 ± 0.017	0.348HisLys: 0.348 ± 0.012	2.465HisLeu: 2.465 ± 0.031	0.341HisMet: 0.341 ± 0.011	0.375HisAsn: 0.375 ± 0.014	1.892HisPro: 1.892 ± 0.034	0.642HisGln: 0.642 ± 0.016	2.217HisArg: 2.217 ± 0.034	0.997HisSer: 0.997 ± 0.023	1.377HisThr: 1.377 ± 0.028	1.727HisVal: 1.727 ± 0.029	0.397HisTrp: 0.397 ± 0.015	0.481HisTyr: 0.481 ± 0.014	0.0HisXaa: 0.0 ± 0.0
Ile	4.537IleAla: 4.537 ± 0.051	0.279IleCys: 0.279 ± 0.01	2.133IleAsp: 2.133 ± 0.036	1.87IleGlu: 1.87 ± 0.03	0.657IlePhe: 0.657 ± 0.018	3.393IleGly: 3.393 ± 0.044	0.601IleHis: 0.601 ± 0.016	0.809IleIle: 0.809 ± 0.022	0.684IleLys: 0.684 ± 0.019	2.339IleLeu: 2.339 ± 0.034	0.416IleMet: 0.416 ± 0.016	0.644IleAsn: 0.644 ± 0.017	1.704IlePro: 1.704 ± 0.025	0.687IleGln: 0.687 ± 0.018	2.152IleArg: 2.152 ± 0.031	1.569IleSer: 1.569 ± 0.025	2.1IleThr: 2.1 ± 0.034	2.601IleVal: 2.601 ± 0.036	0.361IleTrp: 0.361 ± 0.012	0.488IleTyr: 0.488 ± 0.014	0.0IleXaa: 0.0 ± 0.0
Lys	2.854LysAla: 2.854 ± 0.05	0.114LysCys: 0.114 ± 0.007	1.36LysAsp: 1.36 ± 0.031	1.14LysGlu: 1.14 ± 0.023	0.442LysPhe: 0.442 ± 0.013	1.804LysGly: 1.804 ± 0.035	0.42LysHis: 0.42 ± 0.013	0.821LysIle: 0.821 ± 0.021	0.89LysLys: 0.89 ± 0.031	1.932LysLeu: 1.932 ± 0.03	0.38LysMet: 0.38 ± 0.014	0.515LysAsn: 0.515 ± 0.017	1.291LysPro: 1.291 ± 0.026	0.699LysGln: 0.699 ± 0.021	1.403LysArg: 1.403 ± 0.031	1.129LysSer: 1.129 ± 0.027	1.26LysThr: 1.26 ± 0.028	1.82LysVal: 1.82 ± 0.034	0.273LysTrp: 0.273 ± 0.012	0.439LysTyr: 0.439 ± 0.015	0.0LysXaa: 0.0 ± 0.0
Leu	14.886LeuAla: 14.886 ± 0.114	0.857LeuCys: 0.857 ± 0.022	6.686LeuAsp: 6.686 ± 0.062	4.527LeuGlu: 4.527 ± 0.046	2.54LeuPhe: 2.54 ± 0.036	9.329LeuGly: 9.329 ± 0.065	2.388LeuHis: 2.388 ± 0.037	3.074LeuIle: 3.074 ± 0.043	1.992LeuLys: 1.992 ± 0.034	11.247LeuLeu: 11.247 ± 0.096	1.674LeuMet: 1.674 ± 0.033	1.642LeuAsn: 1.642 ± 0.029	6.464LeuPro: 6.464 ± 0.069	2.226LeuGln: 2.226 ± 0.033	8.68LeuArg: 8.68 ± 0.072	5.298LeuSer: 5.298 ± 0.048	6.818LeuThr: 6.818 ± 0.054	8.808LeuVal: 8.808 ± 0.074	1.29LeuTrp: 1.29 ± 0.022	1.805LeuTyr: 1.805 ± 0.03	0.0LeuXaa: 0.0 ± 0.0
Met	2.18MetAla: 2.18 ± 0.025	0.132MetCys: 0.132 ± 0.007	0.863MetAsp: 0.863 ± 0.02	0.725MetGlu: 0.725 ± 0.016	0.444MetPhe: 0.444 ± 0.016	1.26MetGly: 1.26 ± 0.026	0.363MetHis: 0.363 ± 0.012	0.623MetIle: 0.623 ± 0.015	0.418MetLys: 0.418 ± 0.013	1.62MetLeu: 1.62 ± 0.031	0.275MetMet: 0.275 ± 0.011	0.448MetAsn: 0.448 ± 0.013	1.124MetPro: 1.124 ± 0.024	0.456MetGln: 0.456 ± 0.014	1.419MetArg: 1.419 ± 0.025	1.325MetSer: 1.325 ± 0.025	1.549MetThr: 1.549 ± 0.026	1.229MetVal: 1.229 ± 0.024	0.208MetTrp: 0.208 ± 0.009	0.318MetTyr: 0.318 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.125AsnAla: 2.125 ± 0.027	0.167AsnCys: 0.167 ± 0.009	0.943AsnAsp: 0.943 ± 0.021	0.799AsnGlu: 0.799 ± 0.018	0.492AsnPhe: 0.492 ± 0.015	1.858AsnGly: 1.858 ± 0.031	0.395AsnHis: 0.395 ± 0.013	0.635AsnIle: 0.635 ± 0.018	0.402AsnLys: 0.402 ± 0.015	1.589AsnLeu: 1.589 ± 0.032	0.279AsnMet: 0.279 ± 0.011	0.397AsnAsn: 0.397 ± 0.017	1.314AsnPro: 1.314 ± 0.024	0.491AsnGln: 0.491 ± 0.015	1.271AsnArg: 1.271 ± 0.025	0.948AsnSer: 0.948 ± 0.022	1.085AsnThr: 1.085 ± 0.025	1.356AsnVal: 1.356 ± 0.03	0.287AsnTrp: 0.287 ± 0.012	0.393AsnTyr: 0.393 ± 0.013	0.0AsnXaa: 0.0 ± 0.0
Pro	9.14ProAla: 9.14 ± 0.09	0.35ProCys: 0.35 ± 0.012	4.568ProAsp: 4.568 ± 0.054	4.26ProGlu: 4.26 ± 0.044	1.526ProPhe: 1.526 ± 0.024	6.998ProGly: 6.998 ± 0.071	1.48ProHis: 1.48 ± 0.026	1.149ProIle: 1.149 ± 0.021	1.116ProLys: 1.116 ± 0.025	5.431ProLeu: 5.431 ± 0.058	0.985ProMet: 0.985 ± 0.019	0.852ProAsn: 0.852 ± 0.018	3.803ProPro: 3.803 ± 0.058	1.739ProGln: 1.739 ± 0.041	4.272ProArg: 4.272 ± 0.047	3.322ProSer: 3.322 ± 0.039	3.095ProThr: 3.095 ± 0.04	5.569ProVal: 5.569 ± 0.053	0.898ProTrp: 0.898 ± 0.02	1.429ProTyr: 1.429 ± 0.024	0.0ProXaa: 0.0 ± 0.0
Gln	3.642GlnAla: 3.642 ± 0.043	0.182GlnCys: 0.182 ± 0.009	1.481GlnAsp: 1.481 ± 0.025	1.431GlnGlu: 1.431 ± 0.024	0.638GlnPhe: 0.638 ± 0.015	2.301GlnGly: 2.301 ± 0.031	0.629GlnHis: 0.629 ± 0.016	1.028GlnIle: 1.028 ± 0.023	0.605GlnLys: 0.605 ± 0.018	2.954GlnLeu: 2.954 ± 0.034	0.469GlnMet: 0.469 ± 0.015	0.482GlnAsn: 0.482 ± 0.016	1.64GlnPro: 1.64 ± 0.039	1.228GlnGln: 1.228 ± 0.033	2.333GlnArg: 2.333 ± 0.029	1.309GlnSer: 1.309 ± 0.023	1.27GlnThr: 1.27 ± 0.022	2.345GlnVal: 2.345 ± 0.032	0.473GlnTrp: 0.473 ± 0.017	0.616GlnTyr: 0.616 ± 0.016	0.0GlnXaa: 0.0 ± 0.0
Arg	10.115ArgAla: 10.115 ± 0.078	0.599ArgCys: 0.599 ± 0.016	4.41ArgAsp: 4.41 ± 0.051	4.732ArgGlu: 4.732 ± 0.048	2.351ArgPhe: 2.351 ± 0.035	6.073ArgGly: 6.073 ± 0.06	2.303ArgHis: 2.303 ± 0.032	3.283ArgIle: 3.283 ± 0.034	1.639ArgLys: 1.639 ± 0.034	8.949ArgLeu: 8.949 ± 0.078	1.749ArgMet: 1.749 ± 0.028	1.366ArgAsn: 1.366 ± 0.027	5.26ArgPro: 5.26 ± 0.059	2.242ArgGln: 2.242 ± 0.03	8.117ArgArg: 8.117 ± 0.077	4.288ArgSer: 4.288 ± 0.042	5.766ArgThr: 5.766 ± 0.061	5.921ArgVal: 5.921 ± 0.055	1.394ArgTrp: 1.394 ± 0.026	1.777ArgTyr: 1.777 ± 0.029	0.0ArgXaa: 0.0 ± 0.0
Ser	7.055SerAla: 7.055 ± 0.059	0.41SerCys: 0.41 ± 0.013	2.796SerAsp: 2.796 ± 0.032	2.455SerGlu: 2.455 ± 0.04	1.482SerPhe: 1.482 ± 0.029	6.134SerGly: 6.134 ± 0.056	1.026SerHis: 1.026 ± 0.021	1.356SerIle: 1.356 ± 0.028	1.021SerLys: 1.021 ± 0.023	4.879SerLeu: 4.879 ± 0.051	1.06SerMet: 1.06 ± 0.021	0.86SerAsn: 0.86 ± 0.021	3.262SerPro: 3.262 ± 0.041	1.22SerGln: 1.22 ± 0.024	3.818SerArg: 3.818 ± 0.04	2.851SerSer: 2.851 ± 0.042	3.022SerThr: 3.022 ± 0.041	4.392SerVal: 4.392 ± 0.048	0.911SerTrp: 0.911 ± 0.02	1.21SerTyr: 1.21 ± 0.023	0.0SerXaa: 0.0 ± 0.0
Thr	9.113ThrAla: 9.113 ± 0.071	0.445ThrCys: 0.445 ± 0.014	3.7ThrAsp: 3.7 ± 0.042	3.227ThrGlu: 3.227 ± 0.037	1.587ThrPhe: 1.587 ± 0.025	6.866ThrGly: 6.866 ± 0.056	1.252ThrHis: 1.252 ± 0.024	1.574ThrIle: 1.574 ± 0.028	1.134ThrLys: 1.134 ± 0.023	5.701ThrLeu: 5.701 ± 0.061	0.899ThrMet: 0.899 ± 0.018	0.952ThrAsn: 0.952 ± 0.019	4.142ThrPro: 4.142 ± 0.051	1.316ThrGln: 1.316 ± 0.022	4.046ThrArg: 4.046 ± 0.045	3.192ThrSer: 3.192 ± 0.038	3.742ThrThr: 3.742 ± 0.048	6.129ThrVal: 6.129 ± 0.052	0.912ThrTrp: 0.912 ± 0.021	1.339ThrTyr: 1.339 ± 0.025	0.0ThrXaa: 0.0 ± 0.0
Val	10.759ValAla: 10.759 ± 0.072	0.781ValCys: 0.781 ± 0.018	4.947ValAsp: 4.947 ± 0.045	4.668ValGlu: 4.668 ± 0.047	2.419ValPhe: 2.419 ± 0.036	6.543ValGly: 6.543 ± 0.061	2.041ValHis: 2.041 ± 0.029	2.613ValIle: 2.613 ± 0.036	1.705ValLys: 1.705 ± 0.033	9.484ValLeu: 9.484 ± 0.082	1.401ValMet: 1.401 ± 0.025	1.659ValAsn: 1.659 ± 0.029	5.433ValPro: 5.433 ± 0.052	2.118ValGln: 2.118 ± 0.027	7.426ValArg: 7.426 ± 0.067	4.268ValSer: 4.268 ± 0.049	5.619ValThr: 5.619 ± 0.052	8.071ValVal: 8.071 ± 0.074	1.149ValTrp: 1.149 ± 0.024	1.555ValTyr: 1.555 ± 0.027	0.0ValXaa: 0.0 ± 0.0
Trp	1.677TrpAla: 1.677 ± 0.029	0.16TrpCys: 0.16 ± 0.007	0.855TrpAsp: 0.855 ± 0.022	0.734TrpGlu: 0.734 ± 0.019	0.507TrpPhe: 0.507 ± 0.013	1.072TrpGly: 1.072 ± 0.022	0.375TrpHis: 0.375 ± 0.012	0.535TrpIle: 0.535 ± 0.016	0.365TrpLys: 0.365 ± 0.013	1.74TrpLeu: 1.74 ± 0.03	0.273TrpMet: 0.273 ± 0.01	0.422TrpAsn: 0.422 ± 0.015	0.798TrpPro: 0.798 ± 0.019	0.618TrpGln: 0.618 ± 0.015	1.353TrpArg: 1.353 ± 0.027	0.967TrpSer: 0.967 ± 0.025	1.061TrpThr: 1.061 ± 0.024	0.98TrpVal: 0.98 ± 0.021	0.354TrpTrp: 0.354 ± 0.013	0.38TrpTyr: 0.38 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.815TyrAla: 2.815 ± 0.035	0.175TyrCys: 0.175 ± 0.008	1.528TyrAsp: 1.528 ± 0.027	1.29TyrGlu: 1.29 ± 0.025	0.631TyrPhe: 0.631 ± 0.017	2.332TyrGly: 2.332 ± 0.033	0.369TyrHis: 0.369 ± 0.012	0.487TyrIle: 0.487 ± 0.014	0.396TyrLys: 0.396 ± 0.014	2.014TyrLeu: 2.014 ± 0.03	0.243TyrMet: 0.243 ± 0.011	0.393TyrAsn: 0.393 ± 0.013	1.032TyrPro: 1.032 ± 0.021	0.568TyrGln: 0.568 ± 0.017	1.854TyrArg: 1.854 ± 0.031	0.929TyrSer: 0.929 ± 0.019	1.164TyrThr: 1.164 ± 0.024	1.631TyrVal: 1.631 ± 0.028	0.344TyrTrp: 0.344 ± 0.012	0.451TyrTyr: 0.451 ± 0.014	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 7533 proteins (2468910 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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