Alteromonadaceae bacterium M269

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae; unclassified Alteromonadaceae

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4871 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V2ZHA3|A0A4V2ZHA3_9ALTE Uncharacterized protein OS=Alteromonadaceae bacterium M269 OX=2546219 GN=EYS14_05510 PE=4 SV=1
MM1 pKa = 6.48TTLSIGLIMAFQSQADD17 pKa = 3.74QTQMVDD23 pKa = 3.08QPGSVLVGYY32 pKa = 7.51WHH34 pKa = 6.7NFCNADD40 pKa = 3.35GYY42 pKa = 11.21QRR44 pKa = 11.84GNAPCISLTSTNPLYY59 pKa = 11.1NVVNVSFMRR68 pKa = 11.84VNDD71 pKa = 3.82TSQGRR76 pKa = 11.84IPTFEE81 pKa = 5.09LDD83 pKa = 3.26PGAGLSEE90 pKa = 4.06QEE92 pKa = 4.93FINQIGQLNAQGRR105 pKa = 11.84SVLIALGGADD115 pKa = 3.95AHH117 pKa = 7.34IEE119 pKa = 4.04LQTGDD124 pKa = 3.59EE125 pKa = 4.0QAFADD130 pKa = 4.53EE131 pKa = 4.98IIRR134 pKa = 11.84LVEE137 pKa = 4.05TYY139 pKa = 10.83GFDD142 pKa = 4.08GLDD145 pKa = 3.15IDD147 pKa = 5.55LEE149 pKa = 4.31QAAITARR156 pKa = 11.84DD157 pKa = 3.8NQTVIPAALRR167 pKa = 11.84MVKK170 pKa = 10.09DD171 pKa = 3.78HH172 pKa = 6.74YY173 pKa = 10.58RR174 pKa = 11.84AQGKK178 pKa = 9.06NFMITMAPEE187 pKa = 4.17FPYY190 pKa = 9.93LTGAGAYY197 pKa = 9.57VPYY200 pKa = 10.58LQGLEE205 pKa = 4.24GYY207 pKa = 10.35YY208 pKa = 10.88DD209 pKa = 4.57FINPQFYY216 pKa = 10.67NQGGDD221 pKa = 3.18GLYY224 pKa = 9.73IDD226 pKa = 4.17GVGWIAQNNDD236 pKa = 2.95ALKK239 pKa = 10.84EE240 pKa = 3.9EE241 pKa = 4.81FIYY244 pKa = 10.84YY245 pKa = 10.05ISDD248 pKa = 4.59AIANGSRR255 pKa = 11.84GFAQIPAEE263 pKa = 3.8RR264 pKa = 11.84LVFGIPANNDD274 pKa = 2.72AAATGFVADD283 pKa = 4.15PADD286 pKa = 4.28LYY288 pKa = 11.09RR289 pKa = 11.84AFGRR293 pKa = 11.84MRR295 pKa = 11.84EE296 pKa = 4.01QGQPLRR302 pKa = 11.84GIMTWSINWDD312 pKa = 3.3VGTDD316 pKa = 3.68SSGQAYY322 pKa = 10.42NSQFVNDD329 pKa = 4.24YY330 pKa = 8.8GTFIQSQSIPSLDD343 pKa = 3.27GAPILRR349 pKa = 11.84GVADD353 pKa = 3.27IRR355 pKa = 11.84IAQGSSFDD363 pKa = 3.85EE364 pKa = 4.3LSGVSAVDD372 pKa = 3.91NEE374 pKa = 4.79DD375 pKa = 3.35GDD377 pKa = 4.04LTAQIVLTGTVNTQVIDD394 pKa = 4.45DD395 pKa = 3.91YY396 pKa = 11.11TLTYY400 pKa = 10.21TVTDD404 pKa = 3.26SDD406 pKa = 4.54GNQATQARR414 pKa = 11.84VVTVFNNPPTLSGITDD430 pKa = 3.58TAIQTNDD437 pKa = 3.33NFDD440 pKa = 3.79ALTGVTASDD449 pKa = 4.56AEE451 pKa = 4.68DD452 pKa = 4.43GDD454 pKa = 4.23LTSQITVSGNVDD466 pKa = 3.03NSVEE470 pKa = 4.06GQYY473 pKa = 11.51VLTYY477 pKa = 10.08QVSDD481 pKa = 3.66SNGQSVTEE489 pKa = 3.93QRR491 pKa = 11.84TVTVSDD497 pKa = 3.66TPVNCTTEE505 pKa = 3.79WDD507 pKa = 3.64SNAIYY512 pKa = 8.89TQDD515 pKa = 4.69DD516 pKa = 3.65LADD519 pKa = 3.83YY520 pKa = 11.43DD521 pKa = 4.04GMTWRR526 pKa = 11.84AKK528 pKa = 8.34WWTQGNTPSDD538 pKa = 3.98DD539 pKa = 3.96PNGPWEE545 pKa = 4.0LTGDD549 pKa = 4.11SGCTTPNPDD558 pKa = 3.98PTPDD562 pKa = 4.57PDD564 pKa = 3.88PTPDD568 pKa = 4.54PDD570 pKa = 3.63PTPDD574 pKa = 3.37QGNGNYY580 pKa = 10.55ANYY583 pKa = 11.0AEE585 pKa = 4.34GTNYY589 pKa = 9.87QAGDD593 pKa = 3.53RR594 pKa = 11.84VSHH597 pKa = 5.94QGQDD601 pKa = 4.09FEE603 pKa = 5.38CKK605 pKa = 9.82PFPFSGWCSGSASAYY620 pKa = 10.39APNTGTNWQDD630 pKa = 2.25AWIRR634 pKa = 11.84LL635 pKa = 3.79

Molecular weight: 68.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R5H075|A0A4R5H075_9ALTE Protein Smg homolog OS=Alteromonadaceae bacterium M269 OX=2546219 GN=smg PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNIKK11 pKa = 10.11RR12 pKa = 11.84KK13 pKa = 9.04RR14 pKa = 11.84SHH16 pKa = 6.18GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37NGRR28 pKa = 11.84KK29 pKa = 9.2VLASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.27GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.91

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4871

0

4871

1643643

25

9140

337.4

37.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.989 ± 0.034

0.937 ± 0.012

5.847 ± 0.036

6.562 ± 0.046

4.405 ± 0.027

6.64 ± 0.035

2.108 ± 0.018

6.413 ± 0.027

5.376 ± 0.04

10.161 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.288 ± 0.017

4.706 ± 0.027

3.854 ± 0.022

4.522 ± 0.025

4.547 ± 0.021

7.12 ± 0.03

5.384 ± 0.03

6.795 ± 0.025

1.243 ± 0.014

3.104 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski