Rice yellow stunt virus (RYSV) (Rice transitory yellowing virus)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Nucleorhabdovirus

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q86523|NCAP_RYSV Nucleoprotein OS=Rice yellow stunt virus OX=59380 GN=N PE=1 SV=1
MM1 pKa = 7.95SSQQEE6 pKa = 4.46TNDD9 pKa = 3.16KK10 pKa = 11.25SNTQGHH16 pKa = 7.27PEE18 pKa = 3.93TDD20 pKa = 3.56PEE22 pKa = 4.7GKK24 pKa = 8.72TGTDD28 pKa = 2.85TGNTEE33 pKa = 4.86DD34 pKa = 5.37SPPDD38 pKa = 3.56TDD40 pKa = 4.29NVPITDD46 pKa = 4.16DD47 pKa = 4.87AIMDD51 pKa = 3.94DD52 pKa = 5.39VMDD55 pKa = 4.63EE56 pKa = 4.15DD57 pKa = 4.81VKK59 pKa = 11.5EE60 pKa = 3.89EE61 pKa = 4.88DD62 pKa = 4.46IDD64 pKa = 4.15YY65 pKa = 11.23SWIEE69 pKa = 3.99DD70 pKa = 3.64MRR72 pKa = 11.84DD73 pKa = 3.14EE74 pKa = 5.4DD75 pKa = 6.05VDD77 pKa = 4.31AEE79 pKa = 4.29WLFEE83 pKa = 6.64LIDD86 pKa = 4.21EE87 pKa = 4.74CNGWPDD93 pKa = 2.93

Molecular weight: 10.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q98663|MVP_RYSV Movement protein OS=Rice yellow stunt virus OX=59380 GN=3 PE=1 SV=1
MM1 pKa = 7.54ANDD4 pKa = 3.63NVSDD8 pKa = 4.09YY9 pKa = 12.02ANAAPFARR17 pKa = 11.84FANLQNRR24 pKa = 11.84EE25 pKa = 4.06TLNPIGNEE33 pKa = 3.81AKK35 pKa = 10.16EE36 pKa = 3.9IPYY39 pKa = 10.49NRR41 pKa = 11.84DD42 pKa = 2.87QYY44 pKa = 10.44LTWLAEE50 pKa = 4.06GKK52 pKa = 10.04LFQIGALTDD61 pKa = 3.73AEE63 pKa = 5.05IVAAWTTIKK72 pKa = 9.84TAMGNNTFSEE82 pKa = 4.06THH84 pKa = 5.47MRR86 pKa = 11.84SIVKK90 pKa = 9.66IACNLRR96 pKa = 11.84GITPGSTPLLVTYY109 pKa = 9.58NPPQSATWAPAPSTDD124 pKa = 4.27AIYY127 pKa = 10.82SGTPVAGVIIPQNTGAGGEE146 pKa = 4.17DD147 pKa = 4.07TEE149 pKa = 4.8TEE151 pKa = 3.87ASKK154 pKa = 11.09ARR156 pKa = 11.84AIAFICCYY164 pKa = 10.04LLRR167 pKa = 11.84FIVKK171 pKa = 9.05TEE173 pKa = 3.73EE174 pKa = 4.0HH175 pKa = 6.15LTNSLGNLKK184 pKa = 10.04LQYY187 pKa = 10.5SRR189 pKa = 11.84LYY191 pKa = 9.64SAQSATLSNWNPSNTWASRR210 pKa = 11.84VKK212 pKa = 10.79LGFDD216 pKa = 3.24TYY218 pKa = 10.07LTLRR222 pKa = 11.84ATVAYY227 pKa = 10.08NIASADD233 pKa = 3.5ALLVPEE239 pKa = 4.13NVNYY243 pKa = 10.08GLCRR247 pKa = 11.84MLVFQHH253 pKa = 6.73LEE255 pKa = 3.81LSGLQLYY262 pKa = 9.32KK263 pKa = 10.13MAMTLIAHH271 pKa = 6.89FKK273 pKa = 10.48LIEE276 pKa = 4.36PNKK279 pKa = 8.64FLSWIYY285 pKa = 11.05DD286 pKa = 3.8PLSEE290 pKa = 4.96ASIDD294 pKa = 3.73QIYY297 pKa = 9.97KK298 pKa = 9.96IAVNYY303 pKa = 10.89DD304 pKa = 3.13NVNSKK309 pKa = 6.97THH311 pKa = 5.52KK312 pKa = 7.6HH313 pKa = 3.99WKK315 pKa = 7.39YY316 pKa = 10.75AKK318 pKa = 10.06LARR321 pKa = 11.84GQYY324 pKa = 8.37WLNTTVKK331 pKa = 10.09RR332 pKa = 11.84NQFLAYY338 pKa = 9.52ILADD342 pKa = 3.8LEE344 pKa = 4.58LKK346 pKa = 10.78YY347 pKa = 11.14GLAGKK352 pKa = 9.8SDD354 pKa = 3.66YY355 pKa = 11.23SSPKK359 pKa = 9.82RR360 pKa = 11.84MKK362 pKa = 10.02ALSGMPVEE370 pKa = 5.07RR371 pKa = 11.84MTEE374 pKa = 3.64AEE376 pKa = 4.47TISKK380 pKa = 10.27AVEE383 pKa = 3.81QMYY386 pKa = 8.57TAIEE390 pKa = 3.98SAKK393 pKa = 10.55RR394 pKa = 11.84VDD396 pKa = 3.27AGAAYY401 pKa = 10.37RR402 pKa = 11.84LAKK405 pKa = 10.54KK406 pKa = 10.46LGPPRR411 pKa = 11.84ANAHH415 pKa = 5.36SRR417 pKa = 11.84RR418 pKa = 11.84KK419 pKa = 9.5EE420 pKa = 3.95PNNSRR425 pKa = 11.84QHH427 pKa = 6.76RR428 pKa = 11.84DD429 pKa = 3.03KK430 pKa = 11.24QPNSKK435 pKa = 9.37QQGRR439 pKa = 11.84DD440 pKa = 3.04KK441 pKa = 10.99RR442 pKa = 11.84NKK444 pKa = 8.05HH445 pKa = 4.66QVLGRR450 pKa = 11.84HH451 pKa = 6.17SKK453 pKa = 9.79PQAQGPPNKK462 pKa = 9.94QQDD465 pKa = 4.09LGPHH469 pKa = 5.08NNKK472 pKa = 9.54PKK474 pKa = 9.86EE475 pKa = 4.03ASRR478 pKa = 11.84PPQQDD483 pKa = 3.1RR484 pKa = 11.84QQLAQPWRR492 pKa = 11.84LTRR495 pKa = 11.84RR496 pKa = 11.84QRR498 pKa = 11.84GARR501 pKa = 11.84GHH503 pKa = 5.69RR504 pKa = 11.84TQTLSGMFCKK514 pKa = 10.1GTCQCIQQ521 pKa = 2.97

Molecular weight: 58.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

4128

93

1967

589.7

66.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.281 ± 0.954

1.817 ± 0.247

6.105 ± 0.605

5.644 ± 0.406

2.98 ± 0.369

6.032 ± 0.212

2.398 ± 0.342

7.461 ± 0.546

6.202 ± 0.521

8.963 ± 0.714

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.004 ± 0.234

4.918 ± 0.504

4.385 ± 0.353

3.319 ± 0.494

4.869 ± 0.394

8.067 ± 0.575

6.977 ± 0.473

6.008 ± 0.481

1.55 ± 0.163

4.021 ± 0.368
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski