Opitutaceae bacterium TAV4

Taxonomy: cellular organisms; Bacteria; PVC group; Verrucomicrobia; Opitutae; Opitutales; Opitutaceae; unclassified Opitutaceae

Average proteome isoelectric point is 7.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4229 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3R8IQ57|A0A3R8IQ57_9BACT LamG domain-containing protein OS=Opitutaceae bacterium TAV4 OX=278959 GN=Ga0100231_009725 PE=4 SV=1
MM1 pKa = 7.55ANKK4 pKa = 9.83ADD6 pKa = 4.75KK7 pKa = 10.69IEE9 pKa = 3.93QNAAGKK15 pKa = 9.98FYY17 pKa = 11.41VDD19 pKa = 4.16TQCIDD24 pKa = 3.5CDD26 pKa = 4.0LCRR29 pKa = 11.84EE30 pKa = 4.11TAPKK34 pKa = 10.21FFSRR38 pKa = 11.84YY39 pKa = 9.96DD40 pKa = 3.24EE41 pKa = 4.66GGYY44 pKa = 10.41SFVQAQPTSEE54 pKa = 4.3EE55 pKa = 4.18EE56 pKa = 3.73IAQCMEE62 pKa = 4.12ALEE65 pKa = 4.6GCPVEE70 pKa = 5.78AIGSDD75 pKa = 3.39GDD77 pKa = 3.73EE78 pKa = 4.05

Molecular weight: 8.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3R8IWP2|A0A3R8IWP2_9BACT IS630 family transposase OS=Opitutaceae bacterium TAV4 OX=278959 GN=Ga0100231_002985 PE=4 SV=1
MM1 pKa = 7.15QPTFRR6 pKa = 11.84PHH8 pKa = 5.47RR9 pKa = 11.84KK10 pKa = 9.07KK11 pKa = 10.33RR12 pKa = 11.84VRR14 pKa = 11.84KK15 pKa = 8.18IGFRR19 pKa = 11.84ARR21 pKa = 11.84IATKK25 pKa = 10.29GGRR28 pKa = 11.84KK29 pKa = 9.26VIAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.73GRR39 pKa = 11.84KK40 pKa = 8.81RR41 pKa = 11.84LTVAA45 pKa = 4.08

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4229

0

4229

1440389

33

4044

340.6

37.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.278 ± 0.053

0.993 ± 0.013

5.215 ± 0.029

5.024 ± 0.041

3.806 ± 0.025

7.995 ± 0.043

2.418 ± 0.022

5.199 ± 0.032

3.505 ± 0.036

9.903 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.849 ± 0.018

3.388 ± 0.038

5.948 ± 0.042

3.181 ± 0.02

7.099 ± 0.047

5.888 ± 0.035

6.637 ± 0.066

6.483 ± 0.034

1.667 ± 0.018

2.524 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski