Verticillium dahliae chrysovirus 1
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D6QSQ6|D6QSQ6_9VIRU Ovarian tumor protease OS=Verticillium dahliae chrysovirus 1 OX=759389 PE=4 SV=1
MM1 pKa = 7.24 SAYY4 pKa = 9.42 KK5 pKa = 10.1 RR6 pKa = 11.84 VMDD9 pKa = 4.23 YY10 pKa = 10.26 MSSAPDD16 pKa = 3.37 SANHH20 pKa = 5.21 IRR22 pKa = 11.84 LWEE25 pKa = 4.15 AATMDD30 pKa = 3.42 KK31 pKa = 10.16 MRR33 pKa = 11.84 SKK35 pKa = 11.13 VSGLEE40 pKa = 4.02 LEE42 pKa = 4.73 RR43 pKa = 11.84 KK44 pKa = 9.35 SLSPGEE50 pKa = 3.96 RR51 pKa = 11.84 AIIEE55 pKa = 4.3 SLDD58 pKa = 3.63 CQAKK62 pKa = 7.46 FTRR65 pKa = 11.84 GGMVEE70 pKa = 5.22 GITLGAIVDD79 pKa = 3.77 ARR81 pKa = 11.84 SDD83 pKa = 3.56 KK84 pKa = 10.83 GDD86 pKa = 3.87 KK87 pKa = 9.98 IPRR90 pKa = 11.84 VRR92 pKa = 11.84 KK93 pKa = 9.61 IKK95 pKa = 10.15 IDD97 pKa = 3.75 FMSDD101 pKa = 3.8 RR102 pKa = 11.84 VWHH105 pKa = 6.47 LAPAVAQVDD114 pKa = 4.33 YY115 pKa = 10.07 VTRR118 pKa = 11.84 DD119 pKa = 3.23 PAFYY123 pKa = 10.21 TLDD126 pKa = 3.37 TNMKK130 pKa = 10.3 DD131 pKa = 4.37 RR132 pKa = 11.84 IISQCPSLSASQLHH146 pKa = 6.12 EE147 pKa = 4.16 LRR149 pKa = 11.84 VDD151 pKa = 3.52 AVKK154 pKa = 10.65 DD155 pKa = 3.77 ALSMPVDD162 pKa = 3.61 MRR164 pKa = 11.84 HH165 pKa = 6.37 LFMKK169 pKa = 9.28 MALMPWDD176 pKa = 3.65 YY177 pKa = 10.82 TLAATSKK184 pKa = 8.38 VQGMEE189 pKa = 3.73 MAADD193 pKa = 4.15 LFDD196 pKa = 4.45 CRR198 pKa = 11.84 HH199 pKa = 6.26 PNAPADD205 pKa = 3.5 ARR207 pKa = 11.84 MALMSSTRR215 pKa = 11.84 VVIDD219 pKa = 3.32 AEE221 pKa = 4.54 GFSRR225 pKa = 11.84 RR226 pKa = 11.84 EE227 pKa = 3.57 LGLIQLAAQQYY238 pKa = 8.29 PSVWYY243 pKa = 10.29 AGDD246 pKa = 3.59 NMYY249 pKa = 8.36 TACSMDD255 pKa = 3.96 ADD257 pKa = 4.04 DD258 pKa = 4.95 VAIVSDD264 pKa = 4.1 GQIDD268 pKa = 4.1 MDD270 pKa = 4.13 TSGMWGSPDD279 pKa = 3.57 TLYY282 pKa = 11.24 QLIWSVAAKK291 pKa = 9.13 MDD293 pKa = 3.92 SVKK296 pKa = 10.79 CLVEE300 pKa = 3.95 ALTVMRR306 pKa = 11.84 GKK308 pKa = 9.92 CRR310 pKa = 11.84 HH311 pKa = 4.65 MHH313 pKa = 6.31 DD314 pKa = 3.08 LVGRR318 pKa = 11.84 VGSVSVYY325 pKa = 10.69 SGVPLSICKK334 pKa = 9.9 SRR336 pKa = 11.84 GMGQTGPPAIVTQAPGYY353 pKa = 8.18 YY354 pKa = 9.2 SSSIALIADD363 pKa = 3.54 YY364 pKa = 10.67 LYY366 pKa = 11.43 GSMFEE371 pKa = 4.14 ISATSVAEE379 pKa = 4.13 EE380 pKa = 4.11 VGGTGDD386 pKa = 4.61 KK387 pKa = 10.58 ICGAAVASDD396 pKa = 3.35 RR397 pKa = 11.84 LYY399 pKa = 11.31 NGLLRR404 pKa = 11.84 DD405 pKa = 3.28 VGLRR409 pKa = 11.84 HH410 pKa = 6.65 EE411 pKa = 5.24 DD412 pKa = 3.29 GSVNILIKK420 pKa = 10.36 NWCSLTGSPVCWGYY434 pKa = 11.22 GGCLKK439 pKa = 10.69 DD440 pKa = 4.03 YY441 pKa = 10.67 IVALTVEE448 pKa = 4.91 MRR450 pKa = 11.84 AGWDD454 pKa = 3.23 VAIPQLVTLIPFMDD468 pKa = 3.86 TKK470 pKa = 10.93 LGAWGHH476 pKa = 4.99 SRR478 pKa = 11.84 HH479 pKa = 5.52 YY480 pKa = 10.13 RR481 pKa = 11.84 GRR483 pKa = 11.84 PDD485 pKa = 3.21 TFNADD490 pKa = 3.36 EE491 pKa = 4.12 SRR493 pKa = 11.84 EE494 pKa = 3.97 RR495 pKa = 11.84 SEE497 pKa = 4.12 TLAVGAWLMGYY508 pKa = 9.85 RR509 pKa = 11.84 GSRR512 pKa = 11.84 PKK514 pKa = 10.78 LFRR517 pKa = 11.84 NSSAKK522 pKa = 9.9 EE523 pKa = 3.94 SRR525 pKa = 11.84 NQTAAEE531 pKa = 4.11 RR532 pKa = 11.84 EE533 pKa = 4.11 LAAEE537 pKa = 4.43 AGSGLVIGQCRR548 pKa = 11.84 FWIEE552 pKa = 3.79 DD553 pKa = 3.53 SLGGRR558 pKa = 11.84 EE559 pKa = 4.55 DD560 pKa = 3.87 EE561 pKa = 4.62 LEE563 pKa = 4.14 EE564 pKa = 4.67 ASGVLFKK571 pKa = 11.33 SSFPNVKK578 pKa = 9.62 CQLMYY583 pKa = 9.83 DD584 pKa = 3.72 TTTEE588 pKa = 3.7 QWALPQEE595 pKa = 4.43 EE596 pKa = 4.48 KK597 pKa = 10.74 DD598 pKa = 3.34 YY599 pKa = 11.34 TKK601 pKa = 10.28 IVKK604 pKa = 7.96 QTTYY608 pKa = 10.18 GYY610 pKa = 10.76 KK611 pKa = 10.11 PEE613 pKa = 4.24 TEE615 pKa = 4.38 LAPMISGPAPNNRR628 pKa = 11.84 NDD630 pKa = 3.15 MFEE633 pKa = 4.18 SVKK636 pKa = 10.74 AIAKK640 pKa = 7.93 AKK642 pKa = 9.87 PIRR645 pKa = 11.84 PYY647 pKa = 10.34 KK648 pKa = 10.34 GPRR651 pKa = 11.84 PDD653 pKa = 3.38 ISATGLTYY661 pKa = 10.8 VPDD664 pKa = 3.76 YY665 pKa = 10.98 VIEE668 pKa = 4.57 GEE670 pKa = 4.56 VASTMLPLRR679 pKa = 11.84 SRR681 pKa = 11.84 APEE684 pKa = 4.07 LGGLTFKK691 pKa = 11.13 SIDD694 pKa = 3.37 VPGDD698 pKa = 3.47 GRR700 pKa = 11.84 CGLHH704 pKa = 7.6 AILEE708 pKa = 4.4 DD709 pKa = 3.94 LKK711 pKa = 10.51 SHH713 pKa = 5.82 GMISQRR719 pKa = 11.84 DD720 pKa = 3.64 SSLVMQRR727 pKa = 11.84 LDD729 pKa = 3.37 STTAAPSFHH738 pKa = 7.21 SPDD741 pKa = 3.75 DD742 pKa = 3.65 LAGAVIKK749 pKa = 10.22 MGLGLDD755 pKa = 4.29 VITDD759 pKa = 4.42 GQPHH763 pKa = 6.77 SYY765 pKa = 8.83 GTSTNHH771 pKa = 6.35 RR772 pKa = 11.84 VLLRR776 pKa = 11.84 LKK778 pKa = 9.66 DD779 pKa = 3.47 HH780 pKa = 7.42 RR781 pKa = 11.84 YY782 pKa = 7.06 TPIVEE787 pKa = 4.32 SAGGEE792 pKa = 4.14 KK793 pKa = 10.19 EE794 pKa = 4.52 VIVAKK799 pKa = 10.47 HH800 pKa = 5.89 GPNPSDD806 pKa = 3.22 ADD808 pKa = 3.84 YY809 pKa = 10.89 IKK811 pKa = 10.85 RR812 pKa = 11.84 LGEE815 pKa = 4.08 FEE817 pKa = 4.8 QMIGPAA823 pKa = 3.72
Molecular weight: 90.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.61
IPC2_protein 5.588
IPC_protein 5.626
Toseland 5.931
ProMoST 5.931
Dawson 5.83
Bjellqvist 5.868
Wikipedia 5.817
Rodwell 5.804
Grimsley 6.046
Solomon 5.83
Lehninger 5.817
Nozaki 6.071
DTASelect 6.262
Thurlkill 6.224
EMBOSS 6.186
Sillero 6.173
Patrickios 4.151
IPC_peptide 5.842
IPC2_peptide 6.16
IPC2.peptide.svr19 6.157
Protein with the highest isoelectric point:
>tr|D6QSQ6|D6QSQ6_9VIRU Ovarian tumor protease OS=Verticillium dahliae chrysovirus 1 OX=759389 PE=4 SV=1
MM1 pKa = 7.54 APSGSNFFKK10 pKa = 11.03 NLWSDD15 pKa = 3.61 AAQPKK20 pKa = 6.92 TRR22 pKa = 11.84 RR23 pKa = 11.84 RR24 pKa = 11.84 KK25 pKa = 7.69 TRR27 pKa = 11.84 GEE29 pKa = 3.67 KK30 pKa = 9.8 KK31 pKa = 9.34 AARR34 pKa = 11.84 EE35 pKa = 3.72 IRR37 pKa = 11.84 RR38 pKa = 11.84 GVVPDD43 pKa = 3.35 TAGWVSEE50 pKa = 4.27 DD51 pKa = 3.82 RR52 pKa = 11.84 PSKK55 pKa = 10.9 ANLVGIVIPSMFGKK69 pKa = 7.3 TTTANKK75 pKa = 10.13 LGWIDD80 pKa = 3.64 VDD82 pKa = 5.27 DD83 pKa = 6.33 LITQEE88 pKa = 4.71 GRR90 pKa = 11.84 AQSMADD96 pKa = 3.48 FGSKK100 pKa = 9.93 YY101 pKa = 10.6 LSGGWIRR108 pKa = 11.84 AMDD111 pKa = 4.49 EE112 pKa = 4.03 YY113 pKa = 11.71 NRR115 pKa = 11.84 MCEE118 pKa = 3.86 RR119 pKa = 11.84 ALSLLTFSEE128 pKa = 4.17 ATVILGHH135 pKa = 5.73 SVSSLVAIGVAPAMRR150 pKa = 11.84 IIPSQKK156 pKa = 9.07 FFDD159 pKa = 3.74 RR160 pKa = 11.84 VVPTMDD166 pKa = 3.37 KK167 pKa = 11.15 DD168 pKa = 3.74 EE169 pKa = 4.35 EE170 pKa = 4.21 AFALANYY177 pKa = 9.38 NAVRR181 pKa = 11.84 LEE183 pKa = 4.59 DD184 pKa = 4.18 DD185 pKa = 3.46 SSPLVVVDD193 pKa = 4.11 SYY195 pKa = 10.25 EE196 pKa = 3.89 TAGAVLAATTEE207 pKa = 4.28 KK208 pKa = 10.18 IGQKK212 pKa = 10.02 LGKK215 pKa = 8.79 TYY217 pKa = 10.96 VDD219 pKa = 3.97 RR220 pKa = 11.84 DD221 pKa = 4.12 TIIADD226 pKa = 3.58 YY227 pKa = 10.94 NGGKK231 pKa = 7.63 ITRR234 pKa = 11.84 VDD236 pKa = 3.02 ADD238 pKa = 3.36 VYY240 pKa = 10.0 MRR242 pKa = 11.84 EE243 pKa = 4.04 LGRR246 pKa = 11.84 PYY248 pKa = 10.69 KK249 pKa = 10.87 GFGRR253 pKa = 11.84 LAGSWARR260 pKa = 11.84 AVSHH264 pKa = 6.74 VGTQRR269 pKa = 11.84 LRR271 pKa = 11.84 PTKK274 pKa = 9.19 EE275 pKa = 3.55 QTPITPNLLNKK286 pKa = 10.19 AMAVDD291 pKa = 3.93 PEE293 pKa = 4.33 LTKK296 pKa = 10.36 MVVGAKK302 pKa = 9.03 QFEE305 pKa = 4.59 PQTHH309 pKa = 5.74 VEE311 pKa = 3.99 QCLLLIISMEE321 pKa = 4.21 DD322 pKa = 3.08 LPDD325 pKa = 3.58 FRR327 pKa = 11.84 EE328 pKa = 4.32 VVGSLLCTPLNCWVKK343 pKa = 10.67 VMRR346 pKa = 11.84 QVSTLARR353 pKa = 11.84 SSDD356 pKa = 3.4 NYY358 pKa = 11.14 LGFTLCGKK366 pKa = 7.39 EE367 pKa = 3.51 RR368 pKa = 11.84 KK369 pKa = 9.03 MMSEE373 pKa = 4.08 LWLLGNRR380 pKa = 11.84 HH381 pKa = 5.86 LRR383 pKa = 11.84 EE384 pKa = 3.96 LDD386 pKa = 3.33 QLVMDD391 pKa = 5.36 RR392 pKa = 11.84 LHH394 pKa = 6.86 GCGGTYY400 pKa = 10.08 KK401 pKa = 10.3 PEE403 pKa = 4.66 PSTADD408 pKa = 3.4 VLDD411 pKa = 3.76 SMVWVGLKK419 pKa = 10.43 EE420 pKa = 3.95 SGGTVRR426 pKa = 11.84 EE427 pKa = 4.01 EE428 pKa = 3.56 LTRR431 pKa = 11.84 RR432 pKa = 11.84 LRR434 pKa = 11.84 KK435 pKa = 8.72 WVGSWDD441 pKa = 3.69 VADD444 pKa = 4.1 SAGIDD449 pKa = 3.91 AINSASEE456 pKa = 3.73 ALTEE460 pKa = 5.47 GKK462 pKa = 8.26 TPWHH466 pKa = 5.5 RR467 pKa = 11.84 TCGEE471 pKa = 4.04 VYY473 pKa = 10.39 HH474 pKa = 5.99 SVKK477 pKa = 10.56 SKK479 pKa = 10.96 EE480 pKa = 3.36 IGAWLFRR487 pKa = 11.84 LVKK490 pKa = 10.08 ICQDD494 pKa = 3.59 DD495 pKa = 4.09 VVKK498 pKa = 10.74 VLWRR502 pKa = 11.84 EE503 pKa = 3.94 RR504 pKa = 11.84 IEE506 pKa = 4.15 AALSKK511 pKa = 10.23 LLCYY515 pKa = 10.43 VLAAKK520 pKa = 10.52 ASRR523 pKa = 11.84 TQLMKK528 pKa = 10.6 YY529 pKa = 9.13 SVPVMCCDD537 pKa = 4.42 DD538 pKa = 3.83 EE539 pKa = 7.06 AIAAAILSMGLRR551 pKa = 11.84 LEE553 pKa = 4.57 DD554 pKa = 4.08 KK555 pKa = 11.22 GDD557 pKa = 3.73 IGWASQGATAVAEE570 pKa = 4.22 TVSCLEE576 pKa = 3.82 SRR578 pKa = 11.84 VILVAEE584 pKa = 4.48 VAHH587 pKa = 5.8 TVASVEE593 pKa = 3.95 RR594 pKa = 11.84 DD595 pKa = 3.05 KK596 pKa = 11.83 DD597 pKa = 3.57 LVANLWHH604 pKa = 7.42 RR605 pKa = 11.84 ARR607 pKa = 11.84 ASDD610 pKa = 4.41 LSGLTWILRR619 pKa = 11.84 RR620 pKa = 11.84 EE621 pKa = 3.83 FDD623 pKa = 3.91 DD624 pKa = 5.28 NKK626 pKa = 11.31 DD627 pKa = 3.59 KK628 pKa = 11.6 VSDD631 pKa = 3.43 SHH633 pKa = 6.32 IRR635 pKa = 11.84 RR636 pKa = 11.84 YY637 pKa = 9.6 IEE639 pKa = 3.95 HH640 pKa = 7.27 ACTPKK645 pKa = 10.67 ARR647 pKa = 11.84 GGRR650 pKa = 11.84 GLMSARR656 pKa = 11.84 GEE658 pKa = 4.27 TFSPSNGGGLWVQVPSSGGRR678 pKa = 11.84 VRR680 pKa = 11.84 AVLNTRR686 pKa = 11.84 KK687 pKa = 10.12 AGTSLSVGDD696 pKa = 4.45 AKK698 pKa = 10.89 RR699 pKa = 11.84 SGNAMSFGLIGSCLTKK715 pKa = 10.78 GMDD718 pKa = 3.5 RR719 pKa = 11.84 EE720 pKa = 4.29 VLGLVVGRR728 pKa = 11.84 IASTKK733 pKa = 9.77 RR734 pKa = 11.84 KK735 pKa = 9.87 QITFDD740 pKa = 3.4 GAVPEE745 pKa = 4.29 TWEE748 pKa = 5.14 LEE750 pKa = 4.07 DD751 pKa = 5.93 LINDD755 pKa = 4.27 TLRR758 pKa = 11.84 EE759 pKa = 4.1 ALTVLL764 pKa = 4.07
Molecular weight: 84.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.383
IPC2_protein 7.805
IPC_protein 7.614
Toseland 7.278
ProMoST 8.244
Dawson 8.404
Bjellqvist 8.741
Wikipedia 8.317
Rodwell 8.448
Grimsley 7.293
Solomon 8.463
Lehninger 8.478
Nozaki 9.004
DTASelect 8.463
Thurlkill 8.551
EMBOSS 8.609
Sillero 8.873
Patrickios 4.8
IPC_peptide 8.448
IPC2_peptide 7.571
IPC2.peptide.svr19 7.809
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
3711
764
1108
927.8
103.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.3 ± 0.464
1.428 ± 0.155
6.764 ± 0.345
6.306 ± 0.357
2.964 ± 0.542
7.276 ± 0.514
2.21 ± 0.246
4.473 ± 0.312
5.039 ± 0.206
8.892 ± 0.29
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.422 ± 0.33
3.072 ± 0.452
4.069 ± 0.524
3.045 ± 0.251
7.464 ± 0.401
7.222 ± 0.578
5.659 ± 0.198
7.33 ± 0.404
1.778 ± 0.208
3.288 ± 0.398
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here