Sphingomonas sp. TF3

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; unclassified Sphingomonas

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4052 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S0R148|A0A3S0R148_9SPHN Acyltransferase OS=Sphingomonas sp. TF3 OX=2495580 GN=EJC47_15125 PE=4 SV=1
MM1 pKa = 7.42TYY3 pKa = 10.71VVTDD7 pKa = 3.32ACIKK11 pKa = 9.7CKK13 pKa = 10.78YY14 pKa = 7.81MDD16 pKa = 4.28CVEE19 pKa = 4.32VCPVDD24 pKa = 3.79CFYY27 pKa = 11.19EE28 pKa = 4.58GEE30 pKa = 4.18NMLVINPSEE39 pKa = 4.67CIDD42 pKa = 4.07CGVCEE47 pKa = 4.44PEE49 pKa = 4.82CPAEE53 pKa = 5.13AILPDD58 pKa = 3.66TEE60 pKa = 4.82KK61 pKa = 11.1DD62 pKa = 3.53VEE64 pKa = 4.32TWLEE68 pKa = 3.94LNATFSAQWPNVTRR82 pKa = 11.84KK83 pKa = 9.91ADD85 pKa = 3.63QTPADD90 pKa = 3.91ADD92 pKa = 3.76EE93 pKa = 5.14HH94 pKa = 7.76KK95 pKa = 11.34GEE97 pKa = 4.0ADD99 pKa = 3.28KK100 pKa = 11.39YY101 pKa = 10.55DD102 pKa = 3.76KK103 pKa = 11.23YY104 pKa = 11.24FSAEE108 pKa = 3.96PGAGDD113 pKa = 3.38

Molecular weight: 12.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S0QVI6|A0A3S0QVI6_9SPHN 10 kDa chaperonin OS=Sphingomonas sp. TF3 OX=2495580 GN=groS PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.58GFRR19 pKa = 11.84SRR21 pKa = 11.84MATPGGRR28 pKa = 11.84NVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84NRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.0KK41 pKa = 10.58LSAA44 pKa = 4.03

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4052

0

4052

1311996

25

2022

323.8

34.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.847 ± 0.068

0.723 ± 0.01

5.91 ± 0.029

4.919 ± 0.038

3.472 ± 0.025

8.829 ± 0.033

2.016 ± 0.017

4.985 ± 0.025

2.837 ± 0.034

9.958 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.229 ± 0.019

2.44 ± 0.031

5.467 ± 0.032

3.055 ± 0.023

7.307 ± 0.038

5.198 ± 0.033

5.731 ± 0.028

7.443 ± 0.027

1.414 ± 0.015

2.221 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski