Amino acid dipepetide frequency for Sphingomonas sp. TF3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.633AlaAla: 20.633 ± 0.209
0.969AlaCys: 0.969 ± 0.029
7.749AlaAsp: 7.749 ± 0.099
7.274AlaGlu: 7.274 ± 0.092
4.551AlaPhe: 4.551 ± 0.056
11.319AlaGly: 11.319 ± 0.101
2.575AlaHis: 2.575 ± 0.049
7.075AlaIle: 7.075 ± 0.077
4.116AlaLys: 4.116 ± 0.063
14.961AlaLeu: 14.961 ± 0.151
3.861AlaMet: 3.861 ± 0.06
3.246AlaAsn: 3.246 ± 0.054
6.847AlaPro: 6.847 ± 0.091
4.698AlaGln: 4.698 ± 0.066
10.338AlaArg: 10.338 ± 0.122
6.797AlaSer: 6.797 ± 0.077
7.778AlaThr: 7.778 ± 0.085
9.315AlaVal: 9.315 ± 0.099
1.729AlaTrp: 1.729 ± 0.044
2.642AlaTyr: 2.642 ± 0.051
0.0AlaXaa: 0.0 ± 0.0
Cys
0.994CysAla: 0.994 ± 0.031
0.084CysCys: 0.084 ± 0.008
0.455CysAsp: 0.455 ± 0.021
0.335CysGlu: 0.335 ± 0.018
0.258CysPhe: 0.258 ± 0.014
0.823CysGly: 0.823 ± 0.029
0.179CysHis: 0.179 ± 0.012
0.313CysIle: 0.313 ± 0.016
0.153CysLys: 0.153 ± 0.012
0.654CysLeu: 0.654 ± 0.024
0.137CysMet: 0.137 ± 0.011
0.175CysAsn: 0.175 ± 0.011
0.415CysPro: 0.415 ± 0.02
0.162CysGln: 0.162 ± 0.012
0.52CysArg: 0.52 ± 0.02
0.418CysSer: 0.418 ± 0.016
0.363CysThr: 0.363 ± 0.016
0.532CysVal: 0.532 ± 0.019
0.094CysTrp: 0.094 ± 0.008
0.171CysTyr: 0.171 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
8.103AspAla: 8.103 ± 0.102
0.434AspCys: 0.434 ± 0.019
3.244AspAsp: 3.244 ± 0.056
2.889AspGlu: 2.889 ± 0.056
2.065AspPhe: 2.065 ± 0.048
5.453AspGly: 5.453 ± 0.078
1.378AspHis: 1.378 ± 0.03
2.835AspIle: 2.835 ± 0.044
1.53AspLys: 1.53 ± 0.033
5.979AspLeu: 5.979 ± 0.072
1.24AspMet: 1.24 ± 0.031
1.163AspAsn: 1.163 ± 0.034
3.894AspPro: 3.894 ± 0.05
1.838AspGln: 1.838 ± 0.036
4.97AspArg: 4.97 ± 0.068
2.4AspSer: 2.4 ± 0.042
2.979AspThr: 2.979 ± 0.043
4.182AspVal: 4.182 ± 0.057
1.05AspTrp: 1.05 ± 0.031
1.477AspTyr: 1.477 ± 0.04
0.0AspXaa: 0.0 ± 0.0
Glu
7.152GluAla: 7.152 ± 0.102
0.284GluCys: 0.284 ± 0.016
2.461GluAsp: 2.461 ± 0.05
2.312GluGlu: 2.312 ± 0.051
1.31GluPhe: 1.31 ± 0.031
4.072GluGly: 4.072 ± 0.068
1.114GluHis: 1.114 ± 0.028
2.754GluIle: 2.754 ± 0.047
1.549GluLys: 1.549 ± 0.038
4.449GluLeu: 4.449 ± 0.07
1.279GluMet: 1.279 ± 0.03
1.168GluAsn: 1.168 ± 0.031
2.468GluPro: 2.468 ± 0.047
1.909GluGln: 1.909 ± 0.038
4.796GluArg: 4.796 ± 0.08
2.1GluSer: 2.1 ± 0.042
3.129GluThr: 3.129 ± 0.049
3.334GluVal: 3.334 ± 0.051
0.689GluTrp: 0.689 ± 0.026
0.862GluTyr: 0.862 ± 0.025
0.0GluXaa: 0.0 ± 0.0
Phe
4.922PheAla: 4.922 ± 0.074
0.284PheCys: 0.284 ± 0.016
2.681PheAsp: 2.681 ± 0.048
1.815PheGlu: 1.815 ± 0.04
1.231PhePhe: 1.231 ± 0.036
3.562PheGly: 3.562 ± 0.052
0.71PheHis: 0.71 ± 0.023
1.35PheIle: 1.35 ± 0.034
0.886PheLys: 0.886 ± 0.029
3.037PheLeu: 3.037 ± 0.052
0.621PheMet: 0.621 ± 0.027
0.975PheAsn: 0.975 ± 0.03
1.505PhePro: 1.505 ± 0.034
0.923PheGln: 0.923 ± 0.028
2.112PheArg: 2.112 ± 0.042
1.951PheSer: 1.951 ± 0.042
2.066PheThr: 2.066 ± 0.045
2.733PheVal: 2.733 ± 0.047
0.482PheTrp: 0.482 ± 0.018
0.87PheTyr: 0.87 ± 0.03
0.0PheXaa: 0.0 ± 0.0
Gly
10.325GlyAla: 10.325 ± 0.086
0.784GlyCys: 0.784 ± 0.027
4.893GlyAsp: 4.893 ± 0.061
4.355GlyGlu: 4.355 ± 0.064
3.626GlyPhe: 3.626 ± 0.058
8.095GlyGly: 8.095 ± 0.103
1.939GlyHis: 1.939 ± 0.038
4.361GlyIle: 4.361 ± 0.063
3.196GlyLys: 3.196 ± 0.059
8.684GlyLeu: 8.684 ± 0.087
2.168GlyMet: 2.168 ± 0.045
2.139GlyAsn: 2.139 ± 0.049
3.747GlyPro: 3.747 ± 0.061
2.924GlyGln: 2.924 ± 0.047
6.216GlyArg: 6.216 ± 0.068
4.764GlySer: 4.764 ± 0.064
5.2GlyThr: 5.2 ± 0.076
6.781GlyVal: 6.781 ± 0.086
1.686GlyTrp: 1.686 ± 0.041
2.409GlyTyr: 2.409 ± 0.045
0.0GlyXaa: 0.0 ± 0.0
His
2.589HisAla: 2.589 ± 0.048
0.184HisCys: 0.184 ± 0.012
1.264HisAsp: 1.264 ± 0.034
0.878HisGlu: 0.878 ± 0.026
0.847HisPhe: 0.847 ± 0.029
2.015HisGly: 2.015 ± 0.042
0.58HisHis: 0.58 ± 0.022
0.921HisIle: 0.921 ± 0.029
0.417HisLys: 0.417 ± 0.021
1.946HisLeu: 1.946 ± 0.038
0.421HisMet: 0.421 ± 0.02
0.457HisAsn: 0.457 ± 0.019
1.338HisPro: 1.338 ± 0.034
0.543HisGln: 0.543 ± 0.022
1.497HisArg: 1.497 ± 0.036
0.933HisSer: 0.933 ± 0.026
0.809HisThr: 0.809 ± 0.028
1.55HisVal: 1.55 ± 0.038
0.366HisTrp: 0.366 ± 0.018
0.6HisTyr: 0.6 ± 0.025
0.0HisXaa: 0.0 ± 0.0
Ile
8.488IleAla: 8.488 ± 0.084
0.373IleCys: 0.373 ± 0.017
4.04IleAsp: 4.04 ± 0.058
3.375IleGlu: 3.375 ± 0.048
1.377IlePhe: 1.377 ± 0.032
5.207IleGly: 5.207 ± 0.068
0.83IleHis: 0.83 ± 0.024
1.8IleIle: 1.8 ± 0.044
1.211IleLys: 1.211 ± 0.033
3.97IleLeu: 3.97 ± 0.061
0.741IleMet: 0.741 ± 0.027
1.216IleAsn: 1.216 ± 0.036
2.229IlePro: 2.229 ± 0.047
1.056IleGln: 1.056 ± 0.029
2.919IleArg: 2.919 ± 0.047
2.459IleSer: 2.459 ± 0.049
2.662IleThr: 2.662 ± 0.048
4.394IleVal: 4.394 ± 0.059
0.564IleTrp: 0.564 ± 0.023
0.938IleTyr: 0.938 ± 0.027
0.0IleXaa: 0.0 ± 0.0
Lys
3.916LysAla: 3.916 ± 0.07
0.139LysCys: 0.139 ± 0.01
1.462LysAsp: 1.462 ± 0.036
0.986LysGlu: 0.986 ± 0.032
0.76LysPhe: 0.76 ± 0.03
2.425LysGly: 2.425 ± 0.05
0.517LysHis: 0.517 ± 0.023
1.464LysIle: 1.464 ± 0.034
0.905LysLys: 0.905 ± 0.035
3.043LysLeu: 3.043 ± 0.051
0.678LysMet: 0.678 ± 0.022
0.679LysAsn: 0.679 ± 0.022
2.021LysPro: 2.021 ± 0.045
0.81LysGln: 0.81 ± 0.029
2.229LysArg: 2.229 ± 0.047
1.467LysSer: 1.467 ± 0.036
1.789LysThr: 1.789 ± 0.039
2.162LysVal: 2.162 ± 0.044
0.354LysTrp: 0.354 ± 0.017
0.566LysTyr: 0.566 ± 0.024
0.0LysXaa: 0.0 ± 0.0
Leu
14.709LeuAla: 14.709 ± 0.151
0.772LeuCys: 0.772 ± 0.024
6.3LeuAsp: 6.3 ± 0.071
4.473LeuGlu: 4.473 ± 0.073
3.519LeuPhe: 3.519 ± 0.063
8.716LeuGly: 8.716 ± 0.096
1.885LeuHis: 1.885 ± 0.037
4.783LeuIle: 4.783 ± 0.069
2.974LeuLys: 2.974 ± 0.054
9.453LeuLeu: 9.453 ± 0.115
1.859LeuMet: 1.859 ± 0.044
2.378LeuAsn: 2.378 ± 0.043
5.729LeuPro: 5.729 ± 0.072
2.602LeuGln: 2.602 ± 0.045
6.974LeuArg: 6.974 ± 0.084
5.941LeuSer: 5.941 ± 0.078
5.771LeuThr: 5.771 ± 0.066
7.367LeuVal: 7.367 ± 0.086
1.302LeuTrp: 1.302 ± 0.037
2.069LeuTyr: 2.069 ± 0.041
0.0LeuXaa: 0.0 ± 0.0
Met
2.996MetAla: 2.996 ± 0.056
0.136MetCys: 0.136 ± 0.009
0.906MetAsp: 0.906 ± 0.026
0.867MetGlu: 0.867 ± 0.026
0.635MetPhe: 0.635 ± 0.024
1.649MetGly: 1.649 ± 0.037
0.391MetHis: 0.391 ± 0.017
1.282MetIle: 1.282 ± 0.035
0.791MetLys: 0.791 ± 0.026
2.526MetLeu: 2.526 ± 0.045
0.565MetMet: 0.565 ± 0.023
0.608MetAsn: 0.608 ± 0.022
1.396MetPro: 1.396 ± 0.037
0.637MetGln: 0.637 ± 0.022
1.733MetArg: 1.733 ± 0.036
1.319MetSer: 1.319 ± 0.027
1.766MetThr: 1.766 ± 0.036
1.651MetVal: 1.651 ± 0.039
0.183MetTrp: 0.183 ± 0.01
0.248MetTyr: 0.248 ± 0.012
0.0MetXaa: 0.0 ± 0.0
Asn
3.313AsnAla: 3.313 ± 0.059
0.189AsnCys: 0.189 ± 0.012
1.345AsnAsp: 1.345 ± 0.035
0.977AsnGlu: 0.977 ± 0.03
0.886AsnPhe: 0.886 ± 0.031
2.401AsnGly: 2.401 ± 0.051
0.463AsnHis: 0.463 ± 0.022
1.197AsnIle: 1.197 ± 0.034
0.566AsnLys: 0.566 ± 0.022
2.389AsnLeu: 2.389 ± 0.044
0.48AsnMet: 0.48 ± 0.02
0.642AsnAsn: 0.642 ± 0.029
1.756AsnPro: 1.756 ± 0.041
0.712AsnGln: 0.712 ± 0.031
1.72AsnArg: 1.72 ± 0.037
1.15AsnSer: 1.15 ± 0.034
1.24AsnThr: 1.24 ± 0.043
1.868AsnVal: 1.868 ± 0.042
0.386AsnTrp: 0.386 ± 0.017
0.716AsnTyr: 0.716 ± 0.027
0.0AsnXaa: 0.0 ± 0.0
Pro
7.219ProAla: 7.219 ± 0.094
0.335ProCys: 0.335 ± 0.016
3.8ProAsp: 3.8 ± 0.056
3.333ProGlu: 3.333 ± 0.051
1.949ProPhe: 1.949 ± 0.045
4.968ProGly: 4.968 ± 0.063
1.059ProHis: 1.059 ± 0.032
2.654ProIle: 2.654 ± 0.046
1.652ProLys: 1.652 ± 0.044
5.047ProLeu: 5.047 ± 0.064
1.169ProMet: 1.169 ± 0.029
1.338ProAsn: 1.338 ± 0.037
3.03ProPro: 3.03 ± 0.066
1.702ProGln: 1.702 ± 0.036
3.134ProArg: 3.134 ± 0.055
2.88ProSer: 2.88 ± 0.048
3.082ProThr: 3.082 ± 0.049
4.47ProVal: 4.47 ± 0.055
0.704ProTrp: 0.704 ± 0.022
1.149ProTyr: 1.149 ± 0.028
0.0ProXaa: 0.0 ± 0.0
Gln
4.346GlnAla: 4.346 ± 0.059
0.191GlnCys: 0.191 ± 0.012
1.385GlnAsp: 1.385 ± 0.028
1.155GlnGlu: 1.155 ± 0.031
1.017GlnPhe: 1.017 ± 0.029
2.364GlnGly: 2.364 ± 0.045
0.649GlnHis: 0.649 ± 0.023
1.732GlnIle: 1.732 ± 0.035
0.825GlnLys: 0.825 ± 0.027
2.904GlnLeu: 2.904 ± 0.051
0.777GlnMet: 0.777 ± 0.024
0.768GlnAsn: 0.768 ± 0.026
1.831GlnPro: 1.831 ± 0.039
1.135GlnGln: 1.135 ± 0.036
2.612GlnArg: 2.612 ± 0.043
1.716GlnSer: 1.716 ± 0.039
1.816GlnThr: 1.816 ± 0.044
2.274GlnVal: 2.274 ± 0.047
0.426GlnTrp: 0.426 ± 0.018
0.627GlnTyr: 0.627 ± 0.026
0.0GlnXaa: 0.0 ± 0.0
Arg
9.527ArgAla: 9.527 ± 0.105
0.489ArgCys: 0.489 ± 0.022
4.481ArgAsp: 4.481 ± 0.062
3.617ArgGlu: 3.617 ± 0.062
2.986ArgPhe: 2.986 ± 0.051
5.27ArgGly: 5.27 ± 0.061
1.682ArgHis: 1.682 ± 0.04
4.115ArgIle: 4.115 ± 0.059
1.865ArgLys: 1.865 ± 0.045
7.954ArgLeu: 7.954 ± 0.097
1.821ArgMet: 1.821 ± 0.037
1.693ArgAsn: 1.693 ± 0.042
3.726ArgPro: 3.726 ± 0.06
2.342ArgGln: 2.342 ± 0.044
5.618ArgArg: 5.618 ± 0.09
3.718ArgSer: 3.718 ± 0.059
3.851ArgThr: 3.851 ± 0.06
5.143ArgVal: 5.143 ± 0.075
1.231ArgTrp: 1.231 ± 0.033
1.936ArgTyr: 1.936 ± 0.041
0.0ArgXaa: 0.0 ± 0.0
Ser
6.861SerAla: 6.861 ± 0.087
0.367SerCys: 0.367 ± 0.018
3.065SerAsp: 3.065 ± 0.048
2.358SerGlu: 2.358 ± 0.039
2.052SerPhe: 2.052 ± 0.042
5.438SerGly: 5.438 ± 0.069
0.924SerHis: 0.924 ± 0.027
2.755SerIle: 2.755 ± 0.047
1.323SerLys: 1.323 ± 0.036
4.974SerLeu: 4.974 ± 0.065
1.042SerMet: 1.042 ± 0.028
1.389SerAsn: 1.389 ± 0.036
2.955SerPro: 2.955 ± 0.05
1.45SerGln: 1.45 ± 0.034
3.331SerArg: 3.331 ± 0.052
2.78SerSer: 2.78 ± 0.056
2.815SerThr: 2.815 ± 0.05
3.941SerVal: 3.941 ± 0.056
0.771SerTrp: 0.771 ± 0.023
1.389SerTyr: 1.389 ± 0.036
0.0SerXaa: 0.0 ± 0.0
Thr
7.274ThrAla: 7.274 ± 0.082
0.376ThrCys: 0.376 ± 0.018
3.021ThrAsp: 3.021 ± 0.05
2.389ThrGlu: 2.389 ± 0.041
1.901ThrPhe: 1.901 ± 0.043
5.525ThrGly: 5.525 ± 0.068
1.038ThrHis: 1.038 ± 0.03
3.383ThrIle: 3.383 ± 0.053
1.484ThrLys: 1.484 ± 0.033
6.583ThrLeu: 6.583 ± 0.073
1.25ThrMet: 1.25 ± 0.034
1.405ThrAsn: 1.405 ± 0.042
3.802ThrPro: 3.802 ± 0.063
1.617ThrGln: 1.617 ± 0.035
3.67ThrArg: 3.67 ± 0.049
2.879ThrSer: 2.879 ± 0.057
3.304ThrThr: 3.304 ± 0.055
4.559ThrVal: 4.559 ± 0.062
0.62ThrTrp: 0.62 ± 0.021
1.229ThrTyr: 1.229 ± 0.039
0.0ThrXaa: 0.0 ± 0.0
Val
10.846ValAla: 10.846 ± 0.104
0.519ValCys: 0.519 ± 0.018
4.293ValAsp: 4.293 ± 0.053
4.236ValGlu: 4.236 ± 0.059
2.379ValPhe: 2.379 ± 0.046
5.835ValGly: 5.835 ± 0.076
1.345ValHis: 1.345 ± 0.033
3.58ValIle: 3.58 ± 0.056
1.952ValLys: 1.952 ± 0.042
7.133ValLeu: 7.133 ± 0.075
1.533ValMet: 1.533 ± 0.037
1.904ValAsn: 1.904 ± 0.041
4.2ValPro: 4.2 ± 0.052
2.197ValGln: 2.197 ± 0.039
5.381ValArg: 5.381 ± 0.076
4.168ValSer: 4.168 ± 0.059
4.907ValThr: 4.907 ± 0.064
5.739ValVal: 5.739 ± 0.075
0.896ValTrp: 0.896 ± 0.027
1.384ValTyr: 1.384 ± 0.031
0.0ValXaa: 0.0 ± 0.0
Trp
1.408TrpAla: 1.408 ± 0.034
0.127TrpCys: 0.127 ± 0.01
0.687TrpAsp: 0.687 ± 0.022
0.536TrpGlu: 0.536 ± 0.018
0.563TrpPhe: 0.563 ± 0.023
0.987TrpGly: 0.987 ± 0.03
0.405TrpHis: 0.405 ± 0.018
0.677TrpIle: 0.677 ± 0.024
0.434TrpLys: 0.434 ± 0.018
1.738TrpLeu: 1.738 ± 0.041
0.303TrpMet: 0.303 ± 0.013
0.425TrpAsn: 0.425 ± 0.021
0.701TrpPro: 0.701 ± 0.022
0.55TrpGln: 0.55 ± 0.021
1.351TrpArg: 1.351 ± 0.039
0.947TrpSer: 0.947 ± 0.028
0.87TrpThr: 0.87 ± 0.025
0.87TrpVal: 0.87 ± 0.024
0.269TrpTrp: 0.269 ± 0.014
0.294TrpTyr: 0.294 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.851TyrAla: 2.851 ± 0.046
0.197TyrCys: 0.197 ± 0.012
1.572TyrAsp: 1.572 ± 0.033
1.028TyrGlu: 1.028 ± 0.028
0.803TyrPhe: 0.803 ± 0.024
2.168TyrGly: 2.168 ± 0.051
0.495TyrHis: 0.495 ± 0.021
0.821TyrIle: 0.821 ± 0.024
0.545TyrLys: 0.545 ± 0.021
2.152TyrLeu: 2.152 ± 0.039
0.341TyrMet: 0.341 ± 0.018
0.63TyrAsn: 0.63 ± 0.026
1.081TyrPro: 1.081 ± 0.029
0.743TyrGln: 0.743 ± 0.026
1.946TyrArg: 1.946 ± 0.047
1.192TyrSer: 1.192 ± 0.037
1.11TyrThr: 1.11 ± 0.033
1.559TyrVal: 1.559 ± 0.035
0.343TyrTrp: 0.343 ± 0.018
0.633TyrTyr: 0.633 ± 0.026
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4052 proteins (1311996 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski