Fonticella tunisiensis
Average proteome isoelectric point is 6.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2943 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R7K958|A0A4R7K958_9CLOT Uncharacterized protein OS=Fonticella tunisiensis OX=1096341 GN=EDD71_12710 PE=4 SV=1
MM1 pKa = 7.9 DD2 pKa = 4.07 KK3 pKa = 10.2 WICTACGYY11 pKa = 10.93 VYY13 pKa = 10.44 DD14 pKa = 4.94 PEE16 pKa = 5.61 VGDD19 pKa = 4.15 PDD21 pKa = 4.65 GGIAPGTRR29 pKa = 11.84 FEE31 pKa = 6.12 DD32 pKa = 4.32 IPDD35 pKa = 3.56 DD36 pKa = 4.12 WVCPLCGLGKK46 pKa = 10.21 DD47 pKa = 3.83 AFEE50 pKa = 4.6 KK51 pKa = 10.57 MSS53 pKa = 3.39
Molecular weight: 5.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.747
IPC2_protein 3.859
IPC_protein 3.77
Toseland 3.567
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.77
Rodwell 3.605
Grimsley 3.478
Solomon 3.757
Lehninger 3.719
Nozaki 3.923
DTASelect 4.164
Thurlkill 3.656
EMBOSS 3.783
Sillero 3.91
Patrickios 0.693
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.784
Protein with the highest isoelectric point:
>tr|A0A4R7K9I4|A0A4R7K9I4_9CLOT Nucleoside ABC transporter ATP-binding protein OS=Fonticella tunisiensis OX=1096341 GN=EDD71_12530 PE=4 SV=1
MM1 pKa = 7.45 LRR3 pKa = 11.84 TYY5 pKa = 9.8 QPKK8 pKa = 9.46 KK9 pKa = 8.28 RR10 pKa = 11.84 QRR12 pKa = 11.84 KK13 pKa = 8.39 KK14 pKa = 8.49 EE15 pKa = 3.5 HH16 pKa = 6.11 GFRR19 pKa = 11.84 KK20 pKa = 9.69 RR21 pKa = 11.84 MKK23 pKa = 8.73 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 LVLKK32 pKa = 10.32 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.84 GRR39 pKa = 11.84 KK40 pKa = 9.04 RR41 pKa = 11.84 LTAA44 pKa = 4.18
Molecular weight: 5.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.359
IPC2_protein 10.921
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.281
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 12.003
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.043
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2943
0
2943
868126
27
2694
295.0
33.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.421 ± 0.05
1.021 ± 0.015
5.497 ± 0.035
7.42 ± 0.053
4.266 ± 0.03
7.088 ± 0.043
1.484 ± 0.018
9.582 ± 0.049
8.122 ± 0.043
8.928 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.742 ± 0.019
5.158 ± 0.038
3.245 ± 0.028
2.322 ± 0.022
4.581 ± 0.033
5.904 ± 0.036
4.655 ± 0.027
6.84 ± 0.035
0.701 ± 0.014
4.024 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here