Campylobacter volucris
Average proteome isoelectric point is 6.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1515 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C8KZB2|A0A5C8KZB2_9PROT Translation initiation factor IF-3 OS=Campylobacter volucris OX=1031542 GN=infC PE=3 SV=1
MM1 pKa = 7.46 SLLITRR7 pKa = 11.84 DD8 pKa = 3.95 CISCDD13 pKa = 3.11 ACRR16 pKa = 11.84 EE17 pKa = 4.02 EE18 pKa = 5.37 CPDD21 pKa = 3.25 EE22 pKa = 5.52 AIYY25 pKa = 11.1 DD26 pKa = 3.7 NDD28 pKa = 4.81 PIYY31 pKa = 11.14 VIDD34 pKa = 5.27 PDD36 pKa = 4.29 LCTEE40 pKa = 4.33 CVNEE44 pKa = 4.25 FSEE47 pKa = 4.52 PACIVACPVDD57 pKa = 5.49 CIIPDD62 pKa = 3.95 PDD64 pKa = 3.37 NVEE67 pKa = 4.49 SIEE70 pKa = 3.95 EE71 pKa = 3.83 LRR73 pKa = 11.84 LKK75 pKa = 10.54 HH76 pKa = 6.43 KK77 pKa = 11.02 NKK79 pKa = 10.57 ANN81 pKa = 3.47
Molecular weight: 9.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.862
IPC2_protein 3.935
IPC_protein 3.872
Toseland 3.681
ProMoST 4.037
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.783
Rodwell 3.706
Grimsley 3.592
Solomon 3.846
Lehninger 3.795
Nozaki 3.986
DTASelect 4.177
Thurlkill 3.732
EMBOSS 3.795
Sillero 3.999
Patrickios 0.299
IPC_peptide 3.846
IPC2_peptide 3.973
IPC2.peptide.svr19 3.887
Protein with the highest isoelectric point:
>tr|A0A5C8L129|A0A5C8L129_9PROT DNA-deoxyinosine glycosylase OS=Campylobacter volucris OX=1031542 GN=FVD15_07445 PE=4 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.09 QPHH8 pKa = 5.34 NTPKK12 pKa = 10.47 KK13 pKa = 7.58 RR14 pKa = 11.84 THH16 pKa = 5.93 GFRR19 pKa = 11.84 VRR21 pKa = 11.84 MKK23 pKa = 9.07 TKK25 pKa = 10.39 NGRR28 pKa = 11.84 KK29 pKa = 9.3 VINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.58 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.391
IPC2_protein 11.052
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.384
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.106
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 8.993
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1515
0
1515
470377
24
1761
310.5
35.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.515 ± 0.056
1.26 ± 0.024
5.436 ± 0.047
6.857 ± 0.064
5.995 ± 0.058
5.274 ± 0.065
1.644 ± 0.025
9.242 ± 0.057
9.767 ± 0.07
10.496 ± 0.077
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.208 ± 0.03
6.548 ± 0.056
2.583 ± 0.031
3.352 ± 0.037
2.739 ± 0.039
6.327 ± 0.046
4.047 ± 0.043
5.16 ± 0.05
0.617 ± 0.017
3.935 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here