Novosphingobium sp. Gsoil 351

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Novosphingobium; unclassified Novosphingobium

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3350 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I5X545|A0A6I5X545_9SPHN Ribonuclease T OS=Novosphingobium sp. Gsoil 351 OX=2675225 GN=GKE62_11665 PE=3 SV=1
MM1 pKa = 7.37SRR3 pKa = 11.84PFTTLCAVALGMIATSPALAATVLFTGGSSALDD36 pKa = 3.38GTNGNVRR43 pKa = 11.84AFSSGGISVQASAWTWNNDD62 pKa = 3.22VLEE65 pKa = 4.04QAYY68 pKa = 10.38LGYY71 pKa = 10.12YY72 pKa = 10.25SSGLGVTSNSDD83 pKa = 2.97GTGASNNSHH92 pKa = 5.84TVDD95 pKa = 3.61NVGPDD100 pKa = 3.65DD101 pKa = 5.37FILLVFNQLVNISSARR117 pKa = 11.84LTQFDD122 pKa = 3.94VSSTIDD128 pKa = 3.75DD129 pKa = 3.64NDD131 pKa = 3.49ATVSYY136 pKa = 9.08ATVANAFVSPTPATVPINSPLFGALQIGGALQNNDD171 pKa = 3.42YY172 pKa = 9.23TVSGHH177 pKa = 6.22NSAPYY182 pKa = 9.14DD183 pKa = 3.55TMLNSAGLVGNVV195 pKa = 3.16

Molecular weight: 19.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I5X0N0|A0A6I5X0N0_9SPHN Transcriptional regulator OS=Novosphingobium sp. Gsoil 351 OX=2675225 GN=GKE62_14345 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.32GFRR19 pKa = 11.84TRR21 pKa = 11.84SATPGGRR28 pKa = 11.84KK29 pKa = 8.63ILRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84IKK41 pKa = 10.86LSAA44 pKa = 3.84

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3350

0

3350

1048074

41

3106

312.9

33.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.772 ± 0.076

0.87 ± 0.013

5.768 ± 0.036

5.356 ± 0.045

3.614 ± 0.03

9.184 ± 0.065

1.96 ± 0.02

4.785 ± 0.028

2.851 ± 0.035

9.963 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.262 ± 0.021

2.497 ± 0.032

5.407 ± 0.033

2.969 ± 0.022

7.483 ± 0.052

5.219 ± 0.041

5.295 ± 0.05

7.127 ± 0.028

1.457 ± 0.018

2.161 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski