Amino acid dipepetide frequency for Novosphingobium sp. Gsoil 351

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.404AlaAla: 20.404 ± 0.249	1.137AlaCys: 1.137 ± 0.035	7.475AlaAsp: 7.475 ± 0.102	7.767AlaGlu: 7.767 ± 0.106	4.524AlaPhe: 4.524 ± 0.062	12.221AlaGly: 12.221 ± 0.148	2.451AlaHis: 2.451 ± 0.055	6.865AlaIle: 6.865 ± 0.088	4.259AlaLys: 4.259 ± 0.086	14.774AlaLeu: 14.774 ± 0.151	3.877AlaMet: 3.877 ± 0.065	3.243AlaAsn: 3.243 ± 0.065	6.503AlaPro: 6.503 ± 0.1	4.65AlaGln: 4.65 ± 0.079	10.378AlaArg: 10.378 ± 0.139	6.86AlaSer: 6.86 ± 0.099	7.021AlaThr: 7.021 ± 0.129	8.924AlaVal: 8.924 ± 0.102	1.892AlaTrp: 1.892 ± 0.044	2.498AlaTyr: 2.498 ± 0.059	0.0AlaXaa: 0.0 ± 0.0
Cys	1.191CysAla: 1.191 ± 0.043	0.106CysCys: 0.106 ± 0.01	0.591CysAsp: 0.591 ± 0.024	0.469CysGlu: 0.469 ± 0.023	0.303CysPhe: 0.303 ± 0.02	1.019CysGly: 1.019 ± 0.034	0.231CysHis: 0.231 ± 0.02	0.356CysIle: 0.356 ± 0.018	0.194CysLys: 0.194 ± 0.014	0.719CysLeu: 0.719 ± 0.029	0.147CysMet: 0.147 ± 0.013	0.243CysAsn: 0.243 ± 0.016	0.491CysPro: 0.491 ± 0.027	0.212CysGln: 0.212 ± 0.014	0.637CysArg: 0.637 ± 0.026	0.467CysSer: 0.467 ± 0.021	0.414CysThr: 0.414 ± 0.019	0.604CysVal: 0.604 ± 0.026	0.127CysTrp: 0.127 ± 0.011	0.183CysTyr: 0.183 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	7.544AspAla: 7.544 ± 0.102	0.531AspCys: 0.531 ± 0.022	3.26AspAsp: 3.26 ± 0.063	3.416AspGlu: 3.416 ± 0.066	2.219AspPhe: 2.219 ± 0.049	5.566AspGly: 5.566 ± 0.093	1.213AspHis: 1.213 ± 0.04	2.558AspIle: 2.558 ± 0.053	1.691AspLys: 1.691 ± 0.044	5.595AspLeu: 5.595 ± 0.072	1.167AspMet: 1.167 ± 0.034	1.267AspAsn: 1.267 ± 0.033	4.067AspPro: 4.067 ± 0.066	1.676AspGln: 1.676 ± 0.043	4.727AspArg: 4.727 ± 0.069	2.305AspSer: 2.305 ± 0.048	2.45AspThr: 2.45 ± 0.05	3.814AspVal: 3.814 ± 0.066	1.049AspTrp: 1.049 ± 0.034	1.567AspTyr: 1.567 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	7.681GluAla: 7.681 ± 0.118	0.378GluCys: 0.378 ± 0.02	2.612GluAsp: 2.612 ± 0.051	2.611GluGlu: 2.611 ± 0.062	1.715GluPhe: 1.715 ± 0.042	4.285GluGly: 4.285 ± 0.068	1.18GluHis: 1.18 ± 0.044	2.953GluIle: 2.953 ± 0.055	1.661GluLys: 1.661 ± 0.051	5.408GluLeu: 5.408 ± 0.084	1.363GluMet: 1.363 ± 0.038	1.213GluAsn: 1.213 ± 0.037	2.661GluPro: 2.661 ± 0.062	1.851GluGln: 1.851 ± 0.048	5.182GluArg: 5.182 ± 0.078	2.166GluSer: 2.166 ± 0.044	3.042GluThr: 3.042 ± 0.05	3.748GluVal: 3.748 ± 0.07	0.881GluTrp: 0.881 ± 0.032	0.97GluTyr: 0.97 ± 0.038	0.0GluXaa: 0.0 ± 0.0
Phe	5.63PheAla: 5.63 ± 0.076	0.325PheCys: 0.325 ± 0.017	2.637PheAsp: 2.637 ± 0.057	1.947PheGlu: 1.947 ± 0.046	1.269PhePhe: 1.269 ± 0.038	3.863PheGly: 3.863 ± 0.067	0.718PheHis: 0.718 ± 0.025	1.322PheIle: 1.322 ± 0.04	0.901PheLys: 0.901 ± 0.031	2.996PheLeu: 2.996 ± 0.066	0.706PheMet: 0.706 ± 0.023	1.018PheAsn: 1.018 ± 0.036	1.571PhePro: 1.571 ± 0.04	0.864PheGln: 0.864 ± 0.026	2.168PheArg: 2.168 ± 0.042	1.943PheSer: 1.943 ± 0.048	2.021PheThr: 2.021 ± 0.052	2.807PheVal: 2.807 ± 0.056	0.481PheTrp: 0.481 ± 0.025	0.95PheTyr: 0.95 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	10.719GlyAla: 10.719 ± 0.157	0.933GlyCys: 0.933 ± 0.031	5.009GlyAsp: 5.009 ± 0.083	5.375GlyGlu: 5.375 ± 0.082	3.838GlyPhe: 3.838 ± 0.068	9.071GlyGly: 9.071 ± 0.197	1.819GlyHis: 1.819 ± 0.045	4.417GlyIle: 4.417 ± 0.066	3.29GlyLys: 3.29 ± 0.064	8.907GlyLeu: 8.907 ± 0.093	2.321GlyMet: 2.321 ± 0.043	2.491GlyAsn: 2.491 ± 0.081	3.854GlyPro: 3.854 ± 0.058	2.999GlyGln: 2.999 ± 0.05	6.533GlyArg: 6.533 ± 0.079	5.058GlySer: 5.058 ± 0.093	4.999GlyThr: 4.999 ± 0.117	6.164GlyVal: 6.164 ± 0.087	1.677GlyTrp: 1.677 ± 0.034	2.362GlyTyr: 2.362 ± 0.05	0.0GlyXaa: 0.0 ± 0.0
His	2.485HisAla: 2.485 ± 0.06	0.231HisCys: 0.231 ± 0.015	1.197HisAsp: 1.197 ± 0.035	0.999HisGlu: 0.999 ± 0.03	0.783HisPhe: 0.783 ± 0.029	1.955HisGly: 1.955 ± 0.053	0.581HisHis: 0.581 ± 0.027	0.843HisIle: 0.843 ± 0.028	0.429HisLys: 0.429 ± 0.02	1.823HisLeu: 1.823 ± 0.049	0.419HisMet: 0.419 ± 0.018	0.436HisAsn: 0.436 ± 0.018	1.329HisPro: 1.329 ± 0.035	0.578HisGln: 0.578 ± 0.022	1.589HisArg: 1.589 ± 0.041	0.909HisSer: 0.909 ± 0.029	0.738HisThr: 0.738 ± 0.028	1.348HisVal: 1.348 ± 0.039	0.373HisTrp: 0.373 ± 0.02	0.554HisTyr: 0.554 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	7.783IleAla: 7.783 ± 0.084	0.422IleCys: 0.422 ± 0.018	3.851IleAsp: 3.851 ± 0.068	3.353IleGlu: 3.353 ± 0.066	1.378IlePhe: 1.378 ± 0.036	4.853IleGly: 4.853 ± 0.066	0.877IleHis: 0.877 ± 0.029	1.549IleIle: 1.549 ± 0.052	1.179IleLys: 1.179 ± 0.036	3.689IleLeu: 3.689 ± 0.063	0.787IleMet: 0.787 ± 0.028	1.221IleAsn: 1.221 ± 0.043	2.304IlePro: 2.304 ± 0.048	1.076IleGln: 1.076 ± 0.035	2.966IleArg: 2.966 ± 0.05	2.444IleSer: 2.444 ± 0.05	2.313IleThr: 2.313 ± 0.066	4.254IleVal: 4.254 ± 0.064	0.589IleTrp: 0.589 ± 0.023	0.964IleTyr: 0.964 ± 0.029	0.0IleXaa: 0.0 ± 0.0
Lys	3.845LysAla: 3.845 ± 0.072	0.191LysCys: 0.191 ± 0.014	1.451LysAsp: 1.451 ± 0.041	1.052LysGlu: 1.052 ± 0.036	0.935LysPhe: 0.935 ± 0.032	2.494LysGly: 2.494 ± 0.058	0.524LysHis: 0.524 ± 0.027	1.373LysIle: 1.373 ± 0.041	0.885LysLys: 0.885 ± 0.035	3.132LysLeu: 3.132 ± 0.062	0.653LysMet: 0.653 ± 0.022	0.707LysAsn: 0.707 ± 0.029	2.036LysPro: 2.036 ± 0.054	0.815LysGln: 0.815 ± 0.029	2.118LysArg: 2.118 ± 0.045	1.55LysSer: 1.55 ± 0.042	1.613LysThr: 1.613 ± 0.048	2.179LysVal: 2.179 ± 0.052	0.374LysTrp: 0.374 ± 0.02	0.583LysTyr: 0.583 ± 0.024	0.0LysXaa: 0.0 ± 0.0
Leu	15.552LeuAla: 15.552 ± 0.177	0.862LeuCys: 0.862 ± 0.027	6.065LeuAsp: 6.065 ± 0.076	4.936LeuGlu: 4.936 ± 0.079	3.437LeuPhe: 3.437 ± 0.067	8.923LeuGly: 8.923 ± 0.096	1.818LeuHis: 1.818 ± 0.047	4.743LeuIle: 4.743 ± 0.073	2.895LeuLys: 2.895 ± 0.055	9.282LeuLeu: 9.282 ± 0.131	1.927LeuMet: 1.927 ± 0.049	2.376LeuAsn: 2.376 ± 0.063	5.511LeuPro: 5.511 ± 0.08	2.368LeuGln: 2.368 ± 0.052	7.159LeuArg: 7.159 ± 0.095	5.714LeuSer: 5.714 ± 0.088	5.806LeuThr: 5.806 ± 0.094	7.168LeuVal: 7.168 ± 0.086	1.19LeuTrp: 1.19 ± 0.038	1.894LeuTyr: 1.894 ± 0.047	0.0LeuXaa: 0.0 ± 0.0
Met	3.189MetAla: 3.189 ± 0.054	0.142MetCys: 0.142 ± 0.012	1.057MetAsp: 1.057 ± 0.033	0.941MetGlu: 0.941 ± 0.032	0.61MetPhe: 0.61 ± 0.02	1.799MetGly: 1.799 ± 0.044	0.397MetHis: 0.397 ± 0.021	1.272MetIle: 1.272 ± 0.041	0.774MetLys: 0.774 ± 0.031	2.418MetLeu: 2.418 ± 0.059	0.515MetMet: 0.515 ± 0.022	0.614MetAsn: 0.614 ± 0.026	1.457MetPro: 1.457 ± 0.038	0.661MetGln: 0.661 ± 0.025	1.79MetArg: 1.79 ± 0.043	1.378MetSer: 1.378 ± 0.043	1.606MetThr: 1.606 ± 0.041	1.524MetVal: 1.524 ± 0.041	0.22MetTrp: 0.22 ± 0.016	0.254MetTyr: 0.254 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	3.267AsnAla: 3.267 ± 0.072	0.266AsnCys: 0.266 ± 0.017	1.365AsnAsp: 1.365 ± 0.037	1.024AsnGlu: 1.024 ± 0.031	0.978AsnPhe: 0.978 ± 0.037	2.451AsnGly: 2.451 ± 0.073	0.468AsnHis: 0.468 ± 0.02	1.202AsnIle: 1.202 ± 0.043	0.573AsnLys: 0.573 ± 0.029	2.532AsnLeu: 2.532 ± 0.055	0.493AsnMet: 0.493 ± 0.025	0.651AsnAsn: 0.651 ± 0.032	1.778AsnPro: 1.778 ± 0.043	0.723AsnGln: 0.723 ± 0.029	1.876AsnArg: 1.876 ± 0.043	1.239AsnSer: 1.239 ± 0.038	1.184AsnThr: 1.184 ± 0.045	1.863AsnVal: 1.863 ± 0.056	0.385AsnTrp: 0.385 ± 0.019	0.655AsnTyr: 0.655 ± 0.031	0.0AsnXaa: 0.0 ± 0.0
Pro	7.363ProAla: 7.363 ± 0.109	0.349ProCys: 0.349 ± 0.02	3.547ProAsp: 3.547 ± 0.063	3.426ProGlu: 3.426 ± 0.064	2.005ProPhe: 2.005 ± 0.048	5.287ProGly: 5.287 ± 0.08	1.021ProHis: 1.021 ± 0.033	2.294ProIle: 2.294 ± 0.049	1.503ProLys: 1.503 ± 0.04	5.297ProLeu: 5.297 ± 0.082	1.123ProMet: 1.123 ± 0.039	1.288ProAsn: 1.288 ± 0.038	2.943ProPro: 2.943 ± 0.072	1.695ProGln: 1.695 ± 0.038	3.465ProArg: 3.465 ± 0.058	2.848ProSer: 2.848 ± 0.052	2.599ProThr: 2.599 ± 0.051	4.266ProVal: 4.266 ± 0.072	0.693ProTrp: 0.693 ± 0.024	1.053ProTyr: 1.053 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	4.152GlnAla: 4.152 ± 0.068	0.258GlnCys: 0.258 ± 0.018	1.324GlnAsp: 1.324 ± 0.038	1.099GlnGlu: 1.099 ± 0.04	1.177GlnPhe: 1.177 ± 0.041	2.463GlnGly: 2.463 ± 0.048	0.587GlnHis: 0.587 ± 0.024	1.624GlnIle: 1.624 ± 0.039	0.724GlnLys: 0.724 ± 0.027	3.021GlnLeu: 3.021 ± 0.055	0.699GlnMet: 0.699 ± 0.028	0.684GlnAsn: 0.684 ± 0.027	1.785GlnPro: 1.785 ± 0.047	1.024GlnGln: 1.024 ± 0.032	2.444GlnArg: 2.444 ± 0.053	1.65GlnSer: 1.65 ± 0.042	1.603GlnThr: 1.603 ± 0.038	2.297GlnVal: 2.297 ± 0.049	0.443GlnTrp: 0.443 ± 0.023	0.634GlnTyr: 0.634 ± 0.027	0.0GlnXaa: 0.0 ± 0.0
Arg	9.048ArgAla: 9.048 ± 0.114	0.649ArgCys: 0.649 ± 0.028	4.35ArgAsp: 4.35 ± 0.073	4.266ArgGlu: 4.266 ± 0.079	3.176ArgPhe: 3.176 ± 0.057	5.5ArgGly: 5.5 ± 0.074	1.696ArgHis: 1.696 ± 0.045	3.994ArgIle: 3.994 ± 0.062	2.083ArgLys: 2.083 ± 0.051	8.203ArgLeu: 8.203 ± 0.104	1.952ArgMet: 1.952 ± 0.048	1.904ArgAsn: 1.904 ± 0.048	3.837ArgPro: 3.837 ± 0.074	2.517ArgGln: 2.517 ± 0.047	6.009ArgArg: 6.009 ± 0.102	3.877ArgSer: 3.877 ± 0.07	3.671ArgThr: 3.671 ± 0.059	4.979ArgVal: 4.979 ± 0.061	1.234ArgTrp: 1.234 ± 0.038	1.882ArgTyr: 1.882 ± 0.039	0.0ArgXaa: 0.0 ± 0.0
Ser	6.621SerAla: 6.621 ± 0.1	0.428SerCys: 0.428 ± 0.022	3.049SerAsp: 3.049 ± 0.05	2.734SerGlu: 2.734 ± 0.045	2.139SerPhe: 2.139 ± 0.043	5.698SerGly: 5.698 ± 0.106	1.027SerHis: 1.027 ± 0.032	2.488SerIle: 2.488 ± 0.058	1.307SerLys: 1.307 ± 0.041	5.143SerLeu: 5.143 ± 0.077	1.083SerMet: 1.083 ± 0.028	1.332SerAsn: 1.332 ± 0.043	2.931SerPro: 2.931 ± 0.056	1.539SerGln: 1.539 ± 0.04	3.676SerArg: 3.676 ± 0.065	2.908SerSer: 2.908 ± 0.063	2.554SerThr: 2.554 ± 0.059	3.536SerVal: 3.536 ± 0.063	0.736SerTrp: 0.736 ± 0.027	1.259SerTyr: 1.259 ± 0.034	0.0SerXaa: 0.0 ± 0.0
Thr	6.81ThrAla: 6.81 ± 0.139	0.451ThrCys: 0.451 ± 0.019	2.612ThrAsp: 2.612 ± 0.055	2.166ThrGlu: 2.166 ± 0.046	1.87ThrPhe: 1.87 ± 0.043	5.42ThrGly: 5.42 ± 0.127	0.907ThrHis: 0.907 ± 0.031	2.985ThrIle: 2.985 ± 0.068	1.273ThrLys: 1.273 ± 0.04	5.801ThrLeu: 5.801 ± 0.101	1.207ThrMet: 1.207 ± 0.036	1.303ThrAsn: 1.303 ± 0.042	3.346ThrPro: 3.346 ± 0.062	1.331ThrGln: 1.331 ± 0.037	3.526ThrArg: 3.526 ± 0.058	2.815ThrSer: 2.815 ± 0.067	3.048ThrThr: 3.048 ± 0.106	4.271ThrVal: 4.271 ± 0.072	0.632ThrTrp: 0.632 ± 0.025	1.173ThrTyr: 1.173 ± 0.038	0.0ThrXaa: 0.0 ± 0.0
Val	10.198ValAla: 10.198 ± 0.105	0.663ValCys: 0.663 ± 0.028	4.053ValAsp: 4.053 ± 0.062	4.272ValGlu: 4.272 ± 0.071	2.356ValPhe: 2.356 ± 0.053	5.852ValGly: 5.852 ± 0.082	1.271ValHis: 1.271 ± 0.038	3.516ValIle: 3.516 ± 0.073	1.882ValLys: 1.882 ± 0.052	6.958ValLeu: 6.958 ± 0.079	1.475ValMet: 1.475 ± 0.038	1.925ValAsn: 1.925 ± 0.049	3.856ValPro: 3.856 ± 0.06	1.849ValGln: 1.849 ± 0.045	5.297ValArg: 5.297 ± 0.075	3.997ValSer: 3.997 ± 0.068	4.388ValThr: 4.388 ± 0.081	5.19ValVal: 5.19 ± 0.08	0.96ValTrp: 0.96 ± 0.032	1.31ValTyr: 1.31 ± 0.037	0.0ValXaa: 0.0 ± 0.0
Trp	1.488TrpAla: 1.488 ± 0.041	0.142TrpCys: 0.142 ± 0.012	0.707TrpAsp: 0.707 ± 0.026	0.61TrpGlu: 0.61 ± 0.024	0.582TrpPhe: 0.582 ± 0.024	1.028TrpGly: 1.028 ± 0.03	0.359TrpHis: 0.359 ± 0.02	0.665TrpIle: 0.665 ± 0.025	0.446TrpLys: 0.446 ± 0.02	1.845TrpLeu: 1.845 ± 0.047	0.343TrpMet: 0.343 ± 0.019	0.437TrpAsn: 0.437 ± 0.021	0.724TrpPro: 0.724 ± 0.027	0.584TrpGln: 0.584 ± 0.025	1.453TrpArg: 1.453 ± 0.044	0.917TrpSer: 0.917 ± 0.029	0.835TrpThr: 0.835 ± 0.028	0.856TrpVal: 0.856 ± 0.03	0.28TrpTrp: 0.28 ± 0.019	0.274TrpTyr: 0.274 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.754TyrAla: 2.754 ± 0.056	0.239TyrCys: 0.239 ± 0.017	1.467TyrAsp: 1.467 ± 0.039	1.098TyrGlu: 1.098 ± 0.037	0.843TyrPhe: 0.843 ± 0.031	2.088TyrGly: 2.088 ± 0.051	0.459TyrHis: 0.459 ± 0.022	0.832TyrIle: 0.832 ± 0.026	0.563TyrLys: 0.563 ± 0.026	2.082TyrLeu: 2.082 ± 0.05	0.36TyrMet: 0.36 ± 0.016	0.618TyrAsn: 0.618 ± 0.027	1.081TyrPro: 1.081 ± 0.035	0.678TyrGln: 0.678 ± 0.03	1.831TyrArg: 1.831 ± 0.051	1.146TyrSer: 1.146 ± 0.033	1.042TyrThr: 1.042 ± 0.038	1.477TyrVal: 1.477 ± 0.041	0.361TyrTrp: 0.361 ± 0.02	0.593TyrTyr: 0.593 ± 0.024	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3350 proteins (1048074 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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