Siphoviridae sp. ctCJE6

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2W9Q8|A0A5Q2W9Q8_9CAUD Capsid maturation protease OS=Siphoviridae sp. ctCJE6 OX=2656719 PE=4 SV=1
MM1 pKa = 7.75RR2 pKa = 11.84FKK4 pKa = 11.14LLRR7 pKa = 11.84DD8 pKa = 3.29SFVFVFLMTFASLFMNLAIVGQKK31 pKa = 10.65VMGQPAPGPVLDD43 pKa = 3.58MAEE46 pKa = 5.25FIRR49 pKa = 11.84MDD51 pKa = 3.34TTNFNGHH58 pKa = 7.08LSSADD63 pKa = 3.31SDD65 pKa = 3.98VQKK68 pKa = 11.13ALEE71 pKa = 4.17TLDD74 pKa = 4.58DD75 pKa = 4.36LVAGGHH81 pKa = 6.27GNGANCSAGNFPLGVDD97 pKa = 3.31EE98 pKa = 4.93NGAVEE103 pKa = 4.49SCTDD107 pKa = 2.77AWTEE111 pKa = 4.38AEE113 pKa = 4.19NTSAGYY119 pKa = 10.49YY120 pKa = 10.3NSLSDD125 pKa = 4.05LQTAVSNDD133 pKa = 2.89FHH135 pKa = 8.09NLGGTDD141 pKa = 4.34DD142 pKa = 5.21DD143 pKa = 4.93VPDD146 pKa = 4.36SGDD149 pKa = 3.38LGLIDD154 pKa = 3.92TEE156 pKa = 4.92AEE158 pKa = 4.13FEE160 pKa = 4.5SEE162 pKa = 4.44LFPIFTPDD170 pKa = 5.18DD171 pKa = 3.86GALDD175 pKa = 4.99DD176 pKa = 6.78DD177 pKa = 6.42DD178 pKa = 4.4ITDD181 pKa = 4.26DD182 pKa = 4.43ASTSLTDD189 pKa = 3.43TGNIAYY195 pKa = 10.1LNSAEE200 pKa = 4.48NISGVWEE207 pKa = 4.16IQDD210 pKa = 3.46DD211 pKa = 4.06TLLNFGNSADD221 pKa = 3.83GGIQHH226 pKa = 6.31VSSSTTTRR234 pKa = 11.84YY235 pKa = 10.41SGVPFHH241 pKa = 7.01FGADD245 pKa = 3.41GTGADD250 pKa = 3.74VVFNSDD256 pKa = 2.7TSGAQLTYY264 pKa = 10.87DD265 pKa = 4.47PSPANATTGHH275 pKa = 7.92LIQDD279 pKa = 4.26VYY281 pKa = 10.51NGQMVYY287 pKa = 9.79WEE289 pKa = 4.56LSGSNNSTSTSSKK302 pKa = 8.9GHH304 pKa = 6.46LNIDD308 pKa = 3.21VDD310 pKa = 4.35FTGSYY315 pKa = 10.93SSLQGANALYY325 pKa = 10.51FDD327 pKa = 4.64FADD330 pKa = 3.74ARR332 pKa = 11.84NISGSFGATGTRR344 pKa = 11.84IASGKK349 pKa = 8.48IARR352 pKa = 11.84TGTHH356 pKa = 6.06SGSSTAEE363 pKa = 4.02STQMLYY369 pKa = 10.06MDD371 pKa = 4.99MGDD374 pKa = 4.18GMTLSGTPTVSRR386 pKa = 11.84TFADD390 pKa = 3.96FLGTSIQGVSSTGATSAVHH409 pKa = 6.56RR410 pKa = 11.84GVHH413 pKa = 5.47LHH415 pKa = 5.93NGYY418 pKa = 9.85IVFATGGGTHH428 pKa = 6.43TFSHH432 pKa = 6.45YY433 pKa = 11.04GVDD436 pKa = 3.13IANTFSEE443 pKa = 4.89VEE445 pKa = 3.87VSGTVNGTNIGVWYY459 pKa = 9.22HH460 pKa = 5.96PTMSSTPVAEE470 pKa = 4.51YY471 pKa = 10.92AFKK474 pKa = 10.8ADD476 pKa = 3.85RR477 pKa = 11.84SIIALKK483 pKa = 10.66SDD485 pKa = 3.48GSTGAYY491 pKa = 9.63AGGRR495 pKa = 11.84AVFGAGEE502 pKa = 4.06DD503 pKa = 3.54AEE505 pKa = 4.4IYY507 pKa = 10.89YY508 pKa = 10.73DD509 pKa = 3.91GTDD512 pKa = 4.36LIIDD516 pKa = 4.22AKK518 pKa = 10.82LVGSGAVDD526 pKa = 3.0IEE528 pKa = 4.65SEE530 pKa = 5.24LEE532 pKa = 3.92VDD534 pKa = 5.09DD535 pKa = 6.32LNTGGNAGTDD545 pKa = 3.43LCHH548 pKa = 7.1DD549 pKa = 4.08ANGRR553 pKa = 11.84LCACGSCAA561 pKa = 5.03

Molecular weight: 58.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2W9C2|A0A5Q2W9C2_9CAUD Uncharacterized protein OS=Siphoviridae sp. ctCJE6 OX=2656719 PE=4 SV=1
MM1 pKa = 8.01RR2 pKa = 11.84LTKK5 pKa = 10.44NDD7 pKa = 2.98WDD9 pKa = 3.23ILMFYY14 pKa = 10.62LRR16 pKa = 11.84AAVVANVILFSCVLMAHH33 pKa = 6.9AEE35 pKa = 4.46SVQPPHH41 pKa = 6.56NLWKK45 pKa = 10.77GLIAEE50 pKa = 4.7AVSEE54 pKa = 4.51GPEE57 pKa = 4.0GMVAVCLVYY66 pKa = 10.53RR67 pKa = 11.84NRR69 pKa = 11.84LQAGMRR75 pKa = 11.84MGCSGLKK82 pKa = 10.17RR83 pKa = 11.84RR84 pKa = 11.84DD85 pKa = 2.98LDD87 pKa = 3.54QFVARR92 pKa = 11.84QGSRR96 pKa = 11.84YY97 pKa = 8.97EE98 pKa = 4.58RR99 pKa = 11.84MAKK102 pKa = 10.35DD103 pKa = 2.87IVARR107 pKa = 11.84VFSGRR112 pKa = 11.84EE113 pKa = 3.35RR114 pKa = 11.84DD115 pKa = 3.59VTMGATHH122 pKa = 6.44YY123 pKa = 11.09EE124 pKa = 4.2NITAFGMPWWAKK136 pKa = 8.24SMRR139 pKa = 11.84ITARR143 pKa = 11.84IGSHH147 pKa = 5.25TFFKK151 pKa = 11.01

Molecular weight: 17.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

14486

29

833

226.3

25.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.538 ± 0.463

1.319 ± 0.157

6.71 ± 0.255

6.751 ± 0.424

4.687 ± 0.227

7.525 ± 0.32

1.712 ± 0.154

6.917 ± 0.304

6.689 ± 0.521

7.718 ± 0.256

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.651 ± 0.176

4.791 ± 0.172

3.403 ± 0.247

3.189 ± 0.149

5.412 ± 0.345

6.227 ± 0.329

5.164 ± 0.428

6.772 ± 0.259

1.595 ± 0.156

3.231 ± 0.223
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski