Amino acid dipepetide frequency for Siphoviridae sp. ctCJE6

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.559AlaAla: 6.559 ± 1.442
0.621AlaCys: 0.621 ± 0.213
5.661AlaAsp: 5.661 ± 0.806
4.695AlaGlu: 4.695 ± 0.628
3.176AlaPhe: 3.176 ± 0.464
6.766AlaGly: 6.766 ± 0.937
1.036AlaHis: 1.036 ± 0.297
4.349AlaIle: 4.349 ± 0.719
4.073AlaLys: 4.073 ± 0.615
6.42AlaLeu: 6.42 ± 0.775
2.416AlaMet: 2.416 ± 0.447
3.452AlaAsn: 3.452 ± 0.845
2.554AlaPro: 2.554 ± 0.405
3.038AlaGln: 3.038 ± 0.457
4.764AlaArg: 4.764 ± 0.495
3.659AlaSer: 3.659 ± 0.482
3.245AlaThr: 3.245 ± 0.594
5.799AlaVal: 5.799 ± 0.734
1.105AlaTrp: 1.105 ± 0.255
2.002AlaTyr: 2.002 ± 0.37
0.0AlaXaa: 0.0 ± 0.0
Cys
0.69CysAla: 0.69 ± 0.232
0.276CysCys: 0.276 ± 0.131
1.381CysAsp: 1.381 ± 0.472
1.243CysGlu: 1.243 ± 0.31
0.414CysPhe: 0.414 ± 0.185
1.381CysGly: 1.381 ± 0.327
0.414CysHis: 0.414 ± 0.153
0.759CysIle: 0.759 ± 0.298
0.552CysLys: 0.552 ± 0.188
0.759CysLeu: 0.759 ± 0.225
0.552CysMet: 0.552 ± 0.208
0.621CysAsn: 0.621 ± 0.18
0.414CysPro: 0.414 ± 0.185
0.345CysGln: 0.345 ± 0.16
0.759CysArg: 0.759 ± 0.209
0.967CysSer: 0.967 ± 0.322
0.69CysThr: 0.69 ± 0.335
0.759CysVal: 0.759 ± 0.225
0.0CysTrp: 0.0 ± 0.0
0.207CysTyr: 0.207 ± 0.13
0.0CysXaa: 0.0 ± 0.0
Asp
5.109AspAla: 5.109 ± 0.752
1.174AspCys: 1.174 ± 0.426
4.556AspAsp: 4.556 ± 0.612
4.695AspGlu: 4.695 ± 0.559
4.971AspPhe: 4.971 ± 0.638
5.661AspGly: 5.661 ± 0.937
0.69AspHis: 0.69 ± 0.187
4.971AspIle: 4.971 ± 0.596
3.521AspLys: 3.521 ± 0.594
6.213AspLeu: 6.213 ± 0.619
1.105AspMet: 1.105 ± 0.268
2.485AspAsn: 2.485 ± 0.383
2.831AspPro: 2.831 ± 0.428
2.071AspGln: 2.071 ± 0.352
2.623AspArg: 2.623 ± 0.404
3.797AspSer: 3.797 ± 0.524
3.383AspThr: 3.383 ± 0.467
4.349AspVal: 4.349 ± 0.778
0.967AspTrp: 0.967 ± 0.25
1.933AspTyr: 1.933 ± 0.33
0.0AspXaa: 0.0 ± 0.0
Glu
5.178GluAla: 5.178 ± 0.913
0.345GluCys: 0.345 ± 0.192
4.625GluAsp: 4.625 ± 0.669
5.04GluGlu: 5.04 ± 0.747
3.314GluPhe: 3.314 ± 0.499
4.418GluGly: 4.418 ± 0.559
1.243GluHis: 1.243 ± 0.309
5.247GluIle: 5.247 ± 0.589
5.523GluLys: 5.523 ± 0.723
5.661GluLeu: 5.661 ± 0.616
1.726GluMet: 1.726 ± 0.37
3.59GluAsn: 3.59 ± 0.599
2.209GluPro: 2.209 ± 0.413
1.588GluGln: 1.588 ± 0.244
3.452GluArg: 3.452 ± 0.635
4.073GluSer: 4.073 ± 0.665
2.485GluThr: 2.485 ± 0.342
4.211GluVal: 4.211 ± 0.577
1.381GluTrp: 1.381 ± 0.281
2.209GluTyr: 2.209 ± 0.494
0.0GluXaa: 0.0 ± 0.0
Phe
3.176PheAla: 3.176 ± 0.338
0.897PheCys: 0.897 ± 0.255
3.314PheAsp: 3.314 ± 0.549
3.038PheGlu: 3.038 ± 0.432
1.381PhePhe: 1.381 ± 0.279
4.625PheGly: 4.625 ± 0.598
1.174PheHis: 1.174 ± 0.282
2.071PheIle: 2.071 ± 0.301
3.245PheLys: 3.245 ± 0.431
3.659PheLeu: 3.659 ± 0.605
0.967PheMet: 0.967 ± 0.263
3.176PheAsn: 3.176 ± 0.503
1.588PhePro: 1.588 ± 0.294
1.588PheGln: 1.588 ± 0.343
2.002PheArg: 2.002 ± 0.304
2.761PheSer: 2.761 ± 0.342
2.002PheThr: 2.002 ± 0.352
3.728PheVal: 3.728 ± 0.571
0.967PheTrp: 0.967 ± 0.23
1.519PheTyr: 1.519 ± 0.35
0.0PheXaa: 0.0 ± 0.0
Gly
4.487GlyAla: 4.487 ± 0.944
1.381GlyCys: 1.381 ± 0.297
4.764GlyAsp: 4.764 ± 0.793
4.487GlyGlu: 4.487 ± 0.639
2.9GlyPhe: 2.9 ± 0.399
7.042GlyGly: 7.042 ± 0.862
1.726GlyHis: 1.726 ± 0.325
4.971GlyIle: 4.971 ± 0.663
5.592GlyLys: 5.592 ± 0.738
4.625GlyLeu: 4.625 ± 0.514
2.002GlyMet: 2.002 ± 0.385
3.659GlyAsn: 3.659 ± 0.702
1.519GlyPro: 1.519 ± 0.316
2.209GlyGln: 2.209 ± 0.395
4.695GlyArg: 4.695 ± 0.648
4.695GlySer: 4.695 ± 0.775
4.764GlyThr: 4.764 ± 1.231
5.937GlyVal: 5.937 ± 0.722
1.588GlyTrp: 1.588 ± 0.35
2.209GlyTyr: 2.209 ± 0.581
0.0GlyXaa: 0.0 ± 0.0
His
1.519HisAla: 1.519 ± 0.321
0.207HisCys: 0.207 ± 0.106
1.036HisAsp: 1.036 ± 0.263
1.243HisGlu: 1.243 ± 0.255
0.897HisPhe: 0.897 ± 0.321
1.381HisGly: 1.381 ± 0.234
0.483HisHis: 0.483 ± 0.285
0.967HisIle: 0.967 ± 0.268
0.759HisLys: 0.759 ± 0.251
0.897HisLeu: 0.897 ± 0.387
0.414HisMet: 0.414 ± 0.144
0.759HisAsn: 0.759 ± 0.259
0.69HisPro: 0.69 ± 0.187
0.897HisGln: 0.897 ± 0.26
1.243HisArg: 1.243 ± 0.324
0.897HisSer: 0.897 ± 0.311
0.621HisThr: 0.621 ± 0.219
0.967HisVal: 0.967 ± 0.199
0.207HisTrp: 0.207 ± 0.109
1.036HisTyr: 1.036 ± 0.221
0.0HisXaa: 0.0 ± 0.0
Ile
4.349IleAla: 4.349 ± 0.526
0.897IleCys: 0.897 ± 0.266
4.418IleAsp: 4.418 ± 0.62
4.902IleGlu: 4.902 ± 0.723
3.59IlePhe: 3.59 ± 0.348
4.625IleGly: 4.625 ± 0.573
1.657IleHis: 1.657 ± 0.339
4.695IleIle: 4.695 ± 0.747
5.109IleLys: 5.109 ± 0.671
5.454IleLeu: 5.454 ± 0.668
0.828IleMet: 0.828 ± 0.218
3.107IleAsn: 3.107 ± 0.482
3.383IlePro: 3.383 ± 0.523
1.795IleGln: 1.795 ± 0.416
4.004IleArg: 4.004 ± 0.568
4.073IleSer: 4.073 ± 0.679
4.142IleThr: 4.142 ± 0.577
5.178IleVal: 5.178 ± 0.629
1.243IleTrp: 1.243 ± 0.471
1.726IleTyr: 1.726 ± 0.298
0.0IleXaa: 0.0 ± 0.0
Lys
4.073LysAla: 4.073 ± 0.606
0.828LysCys: 0.828 ± 0.233
4.349LysAsp: 4.349 ± 0.532
5.109LysGlu: 5.109 ± 1.141
3.038LysPhe: 3.038 ± 0.5
3.038LysGly: 3.038 ± 0.456
1.036LysHis: 1.036 ± 0.396
6.559LysIle: 6.559 ± 0.908
7.111LysLys: 7.111 ± 1.067
4.073LysLeu: 4.073 ± 0.598
2.485LysMet: 2.485 ± 0.47
4.418LysAsn: 4.418 ± 0.751
1.105LysPro: 1.105 ± 0.308
1.381LysGln: 1.381 ± 0.229
3.452LysArg: 3.452 ± 0.432
3.59LysSer: 3.59 ± 0.394
3.107LysThr: 3.107 ± 0.44
4.211LysVal: 4.211 ± 0.57
1.174LysTrp: 1.174 ± 0.293
2.761LysTyr: 2.761 ± 0.457
0.0LysXaa: 0.0 ± 0.0
Leu
7.042LeuAla: 7.042 ± 0.801
1.243LeuCys: 1.243 ± 0.263
4.211LeuAsp: 4.211 ± 0.598
4.556LeuGlu: 4.556 ± 0.465
3.521LeuPhe: 3.521 ± 0.634
4.971LeuGly: 4.971 ± 0.598
0.828LeuHis: 0.828 ± 0.25
5.109LeuIle: 5.109 ± 0.563
5.868LeuLys: 5.868 ± 0.66
5.316LeuLeu: 5.316 ± 0.534
2.347LeuMet: 2.347 ± 0.419
3.659LeuAsn: 3.659 ± 0.447
2.14LeuPro: 2.14 ± 0.404
2.9LeuGln: 2.9 ± 0.433
4.833LeuArg: 4.833 ± 0.758
6.075LeuSer: 6.075 ± 0.771
4.971LeuThr: 4.971 ± 0.71
4.142LeuVal: 4.142 ± 0.452
1.036LeuTrp: 1.036 ± 0.286
2.416LeuTyr: 2.416 ± 0.495
0.0LeuXaa: 0.0 ± 0.0
Met
2.209MetAla: 2.209 ± 0.311
0.138MetCys: 0.138 ± 0.09
1.381MetAsp: 1.381 ± 0.306
1.657MetGlu: 1.657 ± 0.38
1.243MetPhe: 1.243 ± 0.252
2.071MetGly: 2.071 ± 0.35
0.207MetHis: 0.207 ± 0.107
1.795MetIle: 1.795 ± 0.42
1.864MetLys: 1.864 ± 0.447
1.726MetLeu: 1.726 ± 0.504
0.759MetMet: 0.759 ± 0.332
1.174MetAsn: 1.174 ± 0.261
1.105MetPro: 1.105 ± 0.296
1.036MetGln: 1.036 ± 0.222
1.726MetArg: 1.726 ± 0.454
2.347MetSer: 2.347 ± 0.31
1.588MetThr: 1.588 ± 0.342
1.243MetVal: 1.243 ± 0.292
0.207MetTrp: 0.207 ± 0.12
1.036MetTyr: 1.036 ± 0.285
0.0MetXaa: 0.0 ± 0.0
Asn
3.59AsnAla: 3.59 ± 0.704
1.105AsnCys: 1.105 ± 0.32
3.314AsnAsp: 3.314 ± 0.495
1.864AsnGlu: 1.864 ± 0.358
2.692AsnPhe: 2.692 ± 0.357
3.728AsnGly: 3.728 ± 0.51
0.967AsnHis: 0.967 ± 0.242
3.659AsnIle: 3.659 ± 0.462
3.038AsnLys: 3.038 ± 0.429
4.349AsnLeu: 4.349 ± 0.576
0.483AsnMet: 0.483 ± 0.198
2.623AsnAsn: 2.623 ± 0.475
2.347AsnPro: 2.347 ± 0.365
2.14AsnGln: 2.14 ± 0.317
2.209AsnArg: 2.209 ± 0.524
2.071AsnSer: 2.071 ± 0.487
1.657AsnThr: 1.657 ± 0.408
3.383AsnVal: 3.383 ± 0.495
0.69AsnTrp: 0.69 ± 0.21
2.002AsnTyr: 2.002 ± 0.365
0.0AsnXaa: 0.0 ± 0.0
Pro
3.521ProAla: 3.521 ± 0.404
0.207ProCys: 0.207 ± 0.115
2.969ProAsp: 2.969 ± 0.487
2.416ProGlu: 2.416 ± 0.387
1.381ProPhe: 1.381 ± 0.385
2.278ProGly: 2.278 ± 0.481
0.69ProHis: 0.69 ± 0.198
1.519ProIle: 1.519 ± 0.312
1.933ProLys: 1.933 ± 0.378
2.347ProLeu: 2.347 ± 0.402
0.759ProMet: 0.759 ± 0.174
1.105ProAsn: 1.105 ± 0.271
1.243ProPro: 1.243 ± 0.625
0.759ProGln: 0.759 ± 0.202
1.45ProArg: 1.45 ± 0.392
2.002ProSer: 2.002 ± 0.452
1.795ProThr: 1.795 ± 0.374
4.004ProVal: 4.004 ± 0.458
0.69ProTrp: 0.69 ± 0.23
0.967ProTyr: 0.967 ± 0.287
0.0ProXaa: 0.0 ± 0.0
Gln
1.726GlnAla: 1.726 ± 0.398
0.276GlnCys: 0.276 ± 0.128
2.002GlnAsp: 2.002 ± 0.33
2.14GlnGlu: 2.14 ± 0.387
1.105GlnPhe: 1.105 ± 0.245
1.933GlnGly: 1.933 ± 0.273
0.276GlnHis: 0.276 ± 0.138
3.176GlnIle: 3.176 ± 0.449
2.554GlnLys: 2.554 ± 0.406
2.416GlnLeu: 2.416 ± 0.329
1.312GlnMet: 1.312 ± 0.282
1.381GlnAsn: 1.381 ± 0.28
1.243GlnPro: 1.243 ± 0.265
1.105GlnGln: 1.105 ± 0.244
1.795GlnArg: 1.795 ± 0.311
2.278GlnSer: 2.278 ± 0.331
1.864GlnThr: 1.864 ± 0.421
1.795GlnVal: 1.795 ± 0.289
0.414GlnTrp: 0.414 ± 0.162
1.105GlnTyr: 1.105 ± 0.215
0.0GlnXaa: 0.0 ± 0.0
Arg
3.176ArgAla: 3.176 ± 0.436
0.759ArgCys: 0.759 ± 0.217
3.797ArgAsp: 3.797 ± 0.665
4.833ArgGlu: 4.833 ± 0.533
1.657ArgPhe: 1.657 ± 0.349
3.314ArgGly: 3.314 ± 0.474
0.759ArgHis: 0.759 ± 0.207
4.142ArgIle: 4.142 ± 0.635
3.728ArgLys: 3.728 ± 0.642
5.385ArgLeu: 5.385 ± 0.552
1.588ArgMet: 1.588 ± 0.307
2.761ArgAsn: 2.761 ± 0.417
1.519ArgPro: 1.519 ± 0.29
2.071ArgGln: 2.071 ± 0.305
3.935ArgArg: 3.935 ± 0.665
2.278ArgSer: 2.278 ± 0.521
2.278ArgThr: 2.278 ± 0.359
3.521ArgVal: 3.521 ± 0.449
1.174ArgTrp: 1.174 ± 0.274
1.381ArgTyr: 1.381 ± 0.387
0.0ArgXaa: 0.0 ± 0.0
Ser
5.04SerAla: 5.04 ± 0.949
0.759SerCys: 0.759 ± 0.29
4.418SerAsp: 4.418 ± 0.644
3.314SerGlu: 3.314 ± 0.465
2.761SerPhe: 2.761 ± 0.438
6.282SerGly: 6.282 ± 0.912
0.69SerHis: 0.69 ± 0.244
3.866SerIle: 3.866 ± 0.535
3.521SerLys: 3.521 ± 0.437
5.04SerLeu: 5.04 ± 0.525
2.071SerMet: 2.071 ± 0.352
2.209SerAsn: 2.209 ± 0.403
1.864SerPro: 1.864 ± 0.352
1.657SerGln: 1.657 ± 0.265
2.416SerArg: 2.416 ± 0.388
5.316SerSer: 5.316 ± 0.746
4.487SerThr: 4.487 ± 0.767
4.073SerVal: 4.073 ± 0.507
0.69SerTrp: 0.69 ± 0.2
1.795SerTyr: 1.795 ± 0.351
0.0SerXaa: 0.0 ± 0.0
Thr
5.316ThrAla: 5.316 ± 0.818
0.345ThrCys: 0.345 ± 0.187
3.452ThrAsp: 3.452 ± 0.805
2.831ThrGlu: 2.831 ± 0.398
2.969ThrPhe: 2.969 ± 0.5
4.556ThrGly: 4.556 ± 0.663
0.621ThrHis: 0.621 ± 0.202
4.418ThrIle: 4.418 ± 0.712
2.14ThrLys: 2.14 ± 0.421
3.659ThrLeu: 3.659 ± 0.561
1.726ThrMet: 1.726 ± 0.306
2.278ThrAsn: 2.278 ± 0.404
1.726ThrPro: 1.726 ± 0.346
1.381ThrGln: 1.381 ± 0.231
2.14ThrArg: 2.14 ± 0.399
3.797ThrSer: 3.797 ± 0.661
2.969ThrThr: 2.969 ± 0.52
3.038ThrVal: 3.038 ± 0.561
0.483ThrTrp: 0.483 ± 0.156
1.795ThrTyr: 1.795 ± 0.562
0.0ThrXaa: 0.0 ± 0.0
Val
5.385ValAla: 5.385 ± 0.726
0.828ValCys: 0.828 ± 0.258
4.556ValAsp: 4.556 ± 0.458
5.454ValGlu: 5.454 ± 0.62
3.521ValPhe: 3.521 ± 0.491
4.28ValGly: 4.28 ± 0.505
1.243ValHis: 1.243 ± 0.323
4.418ValIle: 4.418 ± 0.487
4.142ValLys: 4.142 ± 0.568
4.695ValLeu: 4.695 ± 0.519
1.381ValMet: 1.381 ± 0.318
3.521ValAsn: 3.521 ± 0.534
2.761ValPro: 2.761 ± 0.41
2.002ValGln: 2.002 ± 0.333
3.107ValArg: 3.107 ± 0.524
4.902ValSer: 4.902 ± 0.769
3.452ValThr: 3.452 ± 0.621
4.625ValVal: 4.625 ± 0.672
1.105ValTrp: 1.105 ± 0.294
2.347ValTyr: 2.347 ± 0.374
0.0ValXaa: 0.0 ± 0.0
Trp
1.105TrpAla: 1.105 ± 0.266
0.621TrpCys: 0.621 ± 0.256
0.759TrpAsp: 0.759 ± 0.191
0.967TrpGlu: 0.967 ± 0.25
0.483TrpPhe: 0.483 ± 0.193
1.45TrpGly: 1.45 ± 0.491
0.483TrpHis: 0.483 ± 0.232
1.312TrpIle: 1.312 ± 0.344
1.105TrpLys: 1.105 ± 0.222
1.381TrpLeu: 1.381 ± 0.283
0.483TrpMet: 0.483 ± 0.19
0.69TrpAsn: 0.69 ± 0.254
0.414TrpPro: 0.414 ± 0.183
0.483TrpGln: 0.483 ± 0.185
1.45TrpArg: 1.45 ± 0.315
0.828TrpSer: 0.828 ± 0.236
0.414TrpThr: 0.414 ± 0.138
0.897TrpVal: 0.897 ± 0.261
0.138TrpTrp: 0.138 ± 0.093
0.483TrpTyr: 0.483 ± 0.161
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.14TyrAla: 2.14 ± 0.402
0.276TyrCys: 0.276 ± 0.141
2.14TyrAsp: 2.14 ± 0.376
3.038TyrGlu: 3.038 ± 0.708
1.864TyrPhe: 1.864 ± 0.428
1.45TyrGly: 1.45 ± 0.274
0.897TyrHis: 0.897 ± 0.211
1.174TyrIle: 1.174 ± 0.238
1.519TyrLys: 1.519 ± 0.276
3.107TyrLeu: 3.107 ± 0.544
1.036TyrMet: 1.036 ± 0.271
1.243TyrAsn: 1.243 ± 0.313
1.381TyrPro: 1.381 ± 0.27
1.45TyrGln: 1.45 ± 0.279
2.071TyrArg: 2.071 ± 0.396
1.864TyrSer: 1.864 ± 0.327
1.726TyrThr: 1.726 ± 0.306
1.864TyrVal: 1.864 ± 0.304
0.69TyrTrp: 0.69 ± 0.211
1.381TyrTyr: 1.381 ± 0.326
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 64 proteins (14486 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski