Jannaschia sp. (strain CCS1)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Jannaschia; unclassified Jannaschia

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4271 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q28MY1|Q28MY1_JANSC Uncharacterized protein OS=Jannaschia sp. (strain CCS1) OX=290400 GN=Jann_3014 PE=4 SV=1
MM1 pKa = 7.82NIRR4 pKa = 11.84KK5 pKa = 8.91NLTASVSIASMVMASAAPAFADD27 pKa = 4.42GEE29 pKa = 4.71LNLLTWEE36 pKa = 5.49GYY38 pKa = 10.85ADD40 pKa = 4.03PSFIDD45 pKa = 3.9AFTEE49 pKa = 4.15ATGCTVSATYY59 pKa = 10.54VGSNDD64 pKa = 3.5DD65 pKa = 3.56FAPRR69 pKa = 11.84LAAGGGVFDD78 pKa = 6.23LISPSIDD85 pKa = 2.86TTAPLIAAGFVEE97 pKa = 6.47AIDD100 pKa = 3.43TDD102 pKa = 4.11RR103 pKa = 11.84IEE105 pKa = 4.9RR106 pKa = 11.84FDD108 pKa = 4.34EE109 pKa = 4.42IYY111 pKa = 10.9DD112 pKa = 3.66AFRR115 pKa = 11.84TADD118 pKa = 4.34GINADD123 pKa = 3.67GQIYY127 pKa = 9.3GLPYY131 pKa = 9.42AWGAIVFMYY140 pKa = 10.14RR141 pKa = 11.84PDD143 pKa = 3.73MFDD146 pKa = 3.66EE147 pKa = 5.25PPTSIADD154 pKa = 3.49LWDD157 pKa = 3.93PALEE161 pKa = 4.25GRR163 pKa = 11.84VSIWDD168 pKa = 4.0DD169 pKa = 3.15KK170 pKa = 10.9SALYY174 pKa = 10.11VAARR178 pKa = 11.84RR179 pKa = 11.84NGDD182 pKa = 2.94MDD184 pKa = 3.97IYY186 pKa = 11.19NLTDD190 pKa = 3.43AQIAAAQEE198 pKa = 4.19SLLEE202 pKa = 3.79QRR204 pKa = 11.84PNIRR208 pKa = 11.84RR209 pKa = 11.84YY210 pKa = 8.58WSTAGEE216 pKa = 4.15LVDD219 pKa = 5.59LYY221 pKa = 11.4LSGEE225 pKa = 4.31VWVSNTWAGYY235 pKa = 10.0QSALLEE241 pKa = 4.35AEE243 pKa = 3.92GMEE246 pKa = 4.15VVEE249 pKa = 7.15FIPEE253 pKa = 3.85EE254 pKa = 3.97NAEE257 pKa = 3.85GWMDD261 pKa = 2.76SWMIVADD268 pKa = 5.41SPNQDD273 pKa = 3.01CAYY276 pKa = 9.56EE277 pKa = 3.96FLNMSISEE285 pKa = 4.36LGQCGVANVNGYY297 pKa = 10.4SVTNPVAARR306 pKa = 11.84NCMTDD311 pKa = 3.0EE312 pKa = 4.19QFASLHH318 pKa = 5.78QDD320 pKa = 3.01DD321 pKa = 5.1PGYY324 pKa = 10.31IDD326 pKa = 6.32SLLLWEE332 pKa = 4.56NLGPRR337 pKa = 11.84LGDD340 pKa = 4.04YY341 pKa = 10.52VSAWNAVKK349 pKa = 10.45AQQ351 pKa = 3.31

Molecular weight: 38.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q28UQ9|Q28UQ9_JANSC MOSC OS=Jannaschia sp. (strain CCS1) OX=290400 GN=Jann_0636 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNLVRR13 pKa = 11.84KK14 pKa = 8.83HH15 pKa = 4.64RR16 pKa = 11.84HH17 pKa = 3.91GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.37AGRR29 pKa = 11.84KK30 pKa = 8.54ILNARR35 pKa = 11.84RR36 pKa = 11.84ARR38 pKa = 11.84GRR40 pKa = 11.84KK41 pKa = 9.07SLSAA45 pKa = 3.93

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4271

0

4271

1324290

41

3486

310.1

33.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.575 ± 0.046

0.913 ± 0.01

6.449 ± 0.035

5.546 ± 0.037

3.753 ± 0.025

8.798 ± 0.036

2.114 ± 0.021

5.24 ± 0.027

2.551 ± 0.032

9.873 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.859 ± 0.02

2.535 ± 0.02

5.257 ± 0.025

3.169 ± 0.02

6.523 ± 0.037

5.06 ± 0.024

5.877 ± 0.037

7.32 ± 0.028

1.419 ± 0.015

2.171 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski