Apple green crinkle associated virus
Average proteome isoelectric point is 6.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K0M7W4|K0M7W4_9VIRU Movement protein TGB2 OS=Apple green crinkle associated virus OX=1211388 GN=TGB-2 PE=3 SV=1
MM1 pKa = 6.96 EE2 pKa = 4.71 TVLSLLNEE10 pKa = 4.49 FGFEE14 pKa = 3.91 RR15 pKa = 11.84 TVEE18 pKa = 4.05 PLSDD22 pKa = 4.94 PIVVHH27 pKa = 6.55 AVPGSGKK34 pKa = 7.26 TTLIKK39 pKa = 10.32 QALLRR44 pKa = 11.84 NHH46 pKa = 6.73 NIEE49 pKa = 3.94 AVTFGVPEE57 pKa = 3.99 KK58 pKa = 11.17 ANIHH62 pKa = 4.48 GTYY65 pKa = 9.33 IKK67 pKa = 10.43 KK68 pKa = 10.18 ARR70 pKa = 11.84 QGQRR74 pKa = 11.84 GRR76 pKa = 11.84 GNFSILDD83 pKa = 3.72 EE84 pKa = 4.39 YY85 pKa = 11.62 LSGEE89 pKa = 4.15 YY90 pKa = 9.06 STGFNCLFSDD100 pKa = 5.21 PYY102 pKa = 10.8 QNHH105 pKa = 6.32 GDD107 pKa = 3.82 CLRR110 pKa = 11.84 AHH112 pKa = 7.05 FIGRR116 pKa = 11.84 CSHH119 pKa = 6.71 RR120 pKa = 11.84 FGNQTVQLLQGLGYY134 pKa = 10.58 NIASSKK140 pKa = 10.06 QDD142 pKa = 2.73 IVEE145 pKa = 4.22 RR146 pKa = 11.84 KK147 pKa = 10.26 NIFQLVDD154 pKa = 3.38 PEE156 pKa = 4.41 GVIICLEE163 pKa = 3.91 EE164 pKa = 4.15 SVEE167 pKa = 5.95 DD168 pKa = 3.84 FLKK171 pKa = 9.77 WHH173 pKa = 5.32 SVEE176 pKa = 4.66 YY177 pKa = 10.58 KK178 pKa = 10.51 LPCQVRR184 pKa = 11.84 GATYY188 pKa = 10.22 DD189 pKa = 2.86 IVTFIHH195 pKa = 6.51 EE196 pKa = 4.52 KK197 pKa = 9.96 PLEE200 pKa = 4.12 EE201 pKa = 4.75 LVSPDD206 pKa = 3.79 LFVALTRR213 pKa = 11.84 HH214 pKa = 4.74 KK215 pKa = 9.34 TKK217 pKa = 10.8 LVLVTNN223 pKa = 4.96
Molecular weight: 25.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.975
IPC2_protein 5.931
IPC_protein 6.008
Toseland 6.402
ProMoST 6.389
Dawson 6.326
Bjellqvist 6.3
Wikipedia 6.351
Rodwell 6.326
Grimsley 6.547
Solomon 6.326
Lehninger 6.326
Nozaki 6.605
DTASelect 6.795
Thurlkill 6.825
EMBOSS 6.781
Sillero 6.707
Patrickios 4.101
IPC_peptide 6.351
IPC2_peptide 6.678
IPC2.peptide.svr19 6.639
Protein with the highest isoelectric point:
>tr|K0M866|K0M866_9VIRU Movement protein TGBp3 OS=Apple green crinkle associated virus OX=1211388 GN=TGB-3 PE=3 SV=1
MM1 pKa = 7.24 FLKK4 pKa = 10.62 SGLGLAIAAASAAYY18 pKa = 9.83 LVLLLAQQLLMNSQKK33 pKa = 10.36 QCTIVITGEE42 pKa = 4.01 SVSIVGCVYY51 pKa = 10.63 SDD53 pKa = 3.74 AFIEE57 pKa = 4.21 LVKK60 pKa = 10.67 GLKK63 pKa = 9.64 PYY65 pKa = 9.38 YY66 pKa = 10.08 HH67 pKa = 7.57 PLGG70 pKa = 4.07
Molecular weight: 7.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.043
IPC2_protein 6.942
IPC_protein 7.468
Toseland 7.0
ProMoST 7.658
Dawson 7.951
Bjellqvist 7.775
Wikipedia 7.878
Rodwell 7.907
Grimsley 6.942
Solomon 8.214
Lehninger 8.229
Nozaki 8.097
DTASelect 7.98
Thurlkill 8.009
EMBOSS 8.2
Sillero 8.302
Patrickios 0.782
IPC_peptide 8.2
IPC2_peptide 7.497
IPC2.peptide.svr19 7.684
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
2999
70
2183
599.8
67.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.169 ± 0.921
2.401 ± 0.466
4.968 ± 1.198
6.435 ± 0.675
6.069 ± 0.935
6.736 ± 0.749
2.834 ± 0.432
6.335 ± 0.35
6.235 ± 1.278
9.637 ± 1.59
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.034 ± 0.443
4.168 ± 0.272
4.635 ± 1.187
3.434 ± 1.202
4.968 ± 0.575
8.036 ± 1.019
5.035 ± 0.671
6.469 ± 0.896
0.9 ± 0.245
2.501 ± 0.485
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here