Marinomonas piezotolerans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Oceanospirillaceae; Marinomonas

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3665 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A370UAH2|A0A370UAH2_9GAMM RNA polymerase sigma factor OS=Marinomonas piezotolerans OX=2213058 GN=rpoE PE=3 SV=1
MM1 pKa = 7.62KK2 pKa = 10.26IILSPVASDD11 pKa = 3.02HH12 pKa = 5.25TTNVSLAGMILTIDD26 pKa = 3.65EE27 pKa = 4.06QDD29 pKa = 3.29YY30 pKa = 10.57DD31 pKa = 4.25LSVIPEE37 pKa = 4.72GGQAEE42 pKa = 4.29ADD44 pKa = 3.7LPFIGIVTRR53 pKa = 11.84DD54 pKa = 3.4EE55 pKa = 4.03VTIQYY60 pKa = 10.41HH61 pKa = 5.74YY62 pKa = 10.77DD63 pKa = 3.29SSLAEE68 pKa = 4.19PSQPTDD74 pKa = 2.76WADD77 pKa = 3.3YY78 pKa = 9.66TFEE81 pKa = 4.67LEE83 pKa = 4.26EE84 pKa = 5.26GEE86 pKa = 4.76VPCPINWKK94 pKa = 9.33PIEE97 pKa = 4.14EE98 pKa = 4.09AVIHH102 pKa = 7.01DD103 pKa = 4.19IQEE106 pKa = 4.1HH107 pKa = 5.2

Molecular weight: 11.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A370UB11|A0A370UB11_9GAMM Uncharacterized protein OS=Marinomonas piezotolerans OX=2213058 GN=DN730_04980 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.42RR12 pKa = 11.84KK13 pKa = 9.04RR14 pKa = 11.84AHH16 pKa = 6.12GFRR19 pKa = 11.84ARR21 pKa = 11.84SATKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 9.22VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.51QLSAA44 pKa = 3.97

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3665

0

3665

1196071

28

3680

326.3

36.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.86 ± 0.039

1.018 ± 0.012

5.71 ± 0.039

6.351 ± 0.036

4.05 ± 0.027

6.872 ± 0.036

2.294 ± 0.02

6.188 ± 0.029

4.865 ± 0.032

10.401 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.618 ± 0.021

3.905 ± 0.023

4.023 ± 0.025

4.392 ± 0.031

4.946 ± 0.031

6.799 ± 0.039

5.347 ± 0.032

7.118 ± 0.04

1.279 ± 0.015

2.965 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski