Falsibacillus pallidus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Falsibacillus

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3956 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A370GHB1|A0A370GHB1_9BACI RNase_H_2 domain-containing protein OS=Falsibacillus pallidus OX=493781 GN=DFR59_10487 PE=4 SV=1
MM1 pKa = 6.43QTVINIFSIKK11 pKa = 10.01VNSVSNNGSINIGEE25 pKa = 4.53ALHH28 pKa = 6.41NSPTANQKK36 pKa = 9.87SQGQTTAYY44 pKa = 10.15GDD46 pKa = 3.73YY47 pKa = 10.31AAPASAMEE55 pKa = 4.46NVYY58 pKa = 10.23IDD60 pKa = 4.77PDD62 pKa = 4.03LNDD65 pKa = 3.88QGDD68 pKa = 3.71IMDD71 pKa = 4.21NTPVISNQMM80 pKa = 3.3

Molecular weight: 8.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A370GBE4|A0A370GBE4_9BACI 3-oxoacyl-[acyl-carrier protein] reductase OS=Falsibacillus pallidus OX=493781 GN=DFR59_11092 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.59RR3 pKa = 11.84TFQPNKK9 pKa = 8.24RR10 pKa = 11.84KK11 pKa = 9.6RR12 pKa = 11.84SKK14 pKa = 9.83VHH16 pKa = 5.77GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 9.66NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.99VLSAA44 pKa = 4.11

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3956

0

3956

1115423

25

1674

282.0

31.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.077 ± 0.041

0.725 ± 0.013

5.172 ± 0.029

7.53 ± 0.052

4.702 ± 0.035

7.029 ± 0.037

2.123 ± 0.02

7.776 ± 0.036

7.323 ± 0.035

9.721 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.983 ± 0.02

4.261 ± 0.027

3.684 ± 0.028

3.554 ± 0.026

3.941 ± 0.028

6.294 ± 0.032

4.968 ± 0.026

6.637 ± 0.035

1.063 ± 0.016

3.437 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski