Serratia phage Parlo

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Parlovirus; Serratia virus Parlo

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 87 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482MFK3|A0A482MFK3_9CAUD Uncharacterized protein OS=Serratia phage Parlo OX=2557554 GN=CPT_Parlo_046 PE=4 SV=1
MM1 pKa = 7.41FGLFFLLCLSGEE13 pKa = 4.22PEE15 pKa = 4.29CYY17 pKa = 9.87RR18 pKa = 11.84FEE20 pKa = 5.26GYY22 pKa = 10.38VYY24 pKa = 10.21PDD26 pKa = 4.65EE27 pKa = 4.95INCNLDD33 pKa = 2.89IEE35 pKa = 4.89DD36 pKa = 3.68RR37 pKa = 11.84HH38 pKa = 6.22LVRR41 pKa = 11.84SDD43 pKa = 3.59HH44 pKa = 6.76ACLPVDD50 pKa = 3.81AVARR54 pKa = 11.84FPQQ57 pKa = 3.6

Molecular weight: 6.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482MH20|A0A482MH20_9CAUD Uncharacterized protein OS=Serratia phage Parlo OX=2557554 GN=CPT_Parlo_067 PE=4 SV=1
MM1 pKa = 7.41TGHH4 pKa = 7.24LRR6 pKa = 11.84YY7 pKa = 9.44ALKK10 pKa = 10.37GWQCTGPFAFHH21 pKa = 6.27RR22 pKa = 11.84QVAGSQLDD30 pKa = 3.64GFRR33 pKa = 11.84RR34 pKa = 11.84ALCFPVQIGAQRR46 pKa = 11.84AAKK49 pKa = 10.41LFGRR53 pKa = 11.84PFLRR57 pKa = 11.84PVHH60 pKa = 5.55IHH62 pKa = 6.3SPQLQLEE69 pKa = 5.03CIHH72 pKa = 6.69KK73 pKa = 10.83SPGFSSQIMQVLHH86 pKa = 7.26DD87 pKa = 3.9YY88 pKa = 11.2RR89 pKa = 11.84RR90 pKa = 11.84FTGDD94 pKa = 2.32AHH96 pKa = 7.48AIIASGGAGG105 pKa = 3.03

Molecular weight: 11.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

87

0

87

20198

34

3764

232.2

25.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.234 ± 0.467

1.03 ± 0.2

6.347 ± 0.188

6.322 ± 0.301

3.198 ± 0.159

7.605 ± 0.242

1.852 ± 0.19

5.025 ± 0.197

5.357 ± 0.247

7.436 ± 0.257

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.768 ± 0.127

3.891 ± 0.181

4.634 ± 0.215

4.976 ± 0.288

6.199 ± 0.275

5.679 ± 0.176

5.699 ± 0.241

6.189 ± 0.25

1.654 ± 0.141

2.906 ± 0.158
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski