Amino acid dipepetide frequency for Serratia phage Parlo

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.695AlaAla: 15.695 ± 1.285
1.139AlaCys: 1.139 ± 0.288
7.674AlaAsp: 7.674 ± 0.758
8.467AlaGlu: 8.467 ± 0.799
3.218AlaPhe: 3.218 ± 0.444
8.318AlaGly: 8.318 ± 0.812
1.584AlaHis: 1.584 ± 0.309
5.149AlaIle: 5.149 ± 0.426
6.536AlaLys: 6.536 ± 0.706
8.368AlaLeu: 8.368 ± 0.807
3.565AlaMet: 3.565 ± 0.57
3.713AlaAsn: 3.713 ± 0.448
4.308AlaPro: 4.308 ± 0.437
6.239AlaGln: 6.239 ± 0.695
5.743AlaArg: 5.743 ± 0.97
5.446AlaSer: 5.446 ± 0.526
6.041AlaThr: 6.041 ± 0.65
6.783AlaVal: 6.783 ± 0.741
1.436AlaTrp: 1.436 ± 0.276
2.872AlaTyr: 2.872 ± 0.469
0.0AlaXaa: 0.0 ± 0.0
Cys
1.188CysAla: 1.188 ± 0.299
0.347CysCys: 0.347 ± 0.152
0.644CysAsp: 0.644 ± 0.203
0.594CysGlu: 0.594 ± 0.169
0.396CysPhe: 0.396 ± 0.154
0.693CysGly: 0.693 ± 0.243
0.198CysHis: 0.198 ± 0.134
0.693CysIle: 0.693 ± 0.198
0.545CysLys: 0.545 ± 0.164
0.644CysLeu: 0.644 ± 0.219
0.248CysMet: 0.248 ± 0.153
0.297CysAsn: 0.297 ± 0.132
0.297CysPro: 0.297 ± 0.143
0.149CysGln: 0.149 ± 0.093
0.743CysArg: 0.743 ± 0.277
0.248CysSer: 0.248 ± 0.127
0.446CysThr: 0.446 ± 0.136
1.089CysVal: 1.089 ± 0.332
0.347CysTrp: 0.347 ± 0.163
0.495CysTyr: 0.495 ± 0.171
0.0CysXaa: 0.0 ± 0.0
Asp
8.516AspAla: 8.516 ± 0.684
0.495AspCys: 0.495 ± 0.171
3.911AspAsp: 3.911 ± 0.59
4.555AspGlu: 4.555 ± 0.694
2.278AspPhe: 2.278 ± 0.329
5.347AspGly: 5.347 ± 0.425
0.891AspHis: 0.891 ± 0.251
3.07AspIle: 3.07 ± 0.417
3.565AspLys: 3.565 ± 0.388
5.001AspLeu: 5.001 ± 0.509
1.337AspMet: 1.337 ± 0.298
2.575AspAsn: 2.575 ± 0.403
2.872AspPro: 2.872 ± 0.296
2.723AspGln: 2.723 ± 0.388
3.515AspArg: 3.515 ± 0.451
2.971AspSer: 2.971 ± 0.419
2.575AspThr: 2.575 ± 0.339
3.713AspVal: 3.713 ± 0.34
1.139AspTrp: 1.139 ± 0.213
2.426AspTyr: 2.426 ± 0.289
0.0AspXaa: 0.0 ± 0.0
Glu
6.437GluAla: 6.437 ± 0.534
0.594GluCys: 0.594 ± 0.183
3.02GluAsp: 3.02 ± 0.501
4.11GluGlu: 4.11 ± 0.696
1.485GluPhe: 1.485 ± 0.294
4.308GluGly: 4.308 ± 0.567
1.287GluHis: 1.287 ± 0.297
4.01GluIle: 4.01 ± 0.411
3.317GluLys: 3.317 ± 0.465
6.09GluLeu: 6.09 ± 0.662
1.535GluMet: 1.535 ± 0.271
2.921GluAsn: 2.921 ± 0.319
2.674GluPro: 2.674 ± 0.461
3.911GluGln: 3.911 ± 0.606
4.803GluArg: 4.803 ± 0.576
3.565GluSer: 3.565 ± 0.36
3.07GluThr: 3.07 ± 0.581
3.515GluVal: 3.515 ± 0.385
1.139GluTrp: 1.139 ± 0.221
1.436GluTyr: 1.436 ± 0.336
0.0GluXaa: 0.0 ± 0.0
Phe
2.822PheAla: 2.822 ± 0.344
0.347PheCys: 0.347 ± 0.135
2.377PheAsp: 2.377 ± 0.303
1.733PheGlu: 1.733 ± 0.264
1.238PhePhe: 1.238 ± 0.239
2.525PheGly: 2.525 ± 0.249
0.99PheHis: 0.99 ± 0.214
1.733PheIle: 1.733 ± 0.308
1.832PheLys: 1.832 ± 0.307
2.278PheLeu: 2.278 ± 0.346
0.842PheMet: 0.842 ± 0.208
1.485PheAsn: 1.485 ± 0.321
1.337PhePro: 1.337 ± 0.278
1.139PheGln: 1.139 ± 0.305
1.683PheArg: 1.683 ± 0.369
2.327PheSer: 2.327 ± 0.273
1.881PheThr: 1.881 ± 0.318
2.03PheVal: 2.03 ± 0.376
0.248PheTrp: 0.248 ± 0.142
1.139PheTyr: 1.139 ± 0.23
0.0PheXaa: 0.0 ± 0.0
Gly
6.239GlyAla: 6.239 ± 0.668
0.693GlyCys: 0.693 ± 0.22
5.248GlyAsp: 5.248 ± 0.532
4.654GlyGlu: 4.654 ± 0.501
2.525GlyPhe: 2.525 ± 0.382
5.941GlyGly: 5.941 ± 0.629
1.337GlyHis: 1.337 ± 0.269
4.11GlyIle: 4.11 ± 0.426
5.991GlyLys: 5.991 ± 0.531
4.902GlyLeu: 4.902 ± 0.442
3.07GlyMet: 3.07 ± 0.446
3.07GlyAsn: 3.07 ± 0.484
1.832GlyPro: 1.832 ± 0.368
3.565GlyGln: 3.565 ± 0.493
4.159GlyArg: 4.159 ± 0.433
4.456GlySer: 4.456 ± 0.506
5.248GlyThr: 5.248 ± 0.584
4.803GlyVal: 4.803 ± 0.559
1.535GlyTrp: 1.535 ± 0.324
2.674GlyTyr: 2.674 ± 0.392
0.0GlyXaa: 0.0 ± 0.0
His
1.584HisAla: 1.584 ± 0.278
0.644HisCys: 0.644 ± 0.251
1.238HisAsp: 1.238 ± 0.215
1.04HisGlu: 1.04 ± 0.227
0.792HisPhe: 0.792 ± 0.197
1.386HisGly: 1.386 ± 0.299
0.693HisHis: 0.693 ± 0.188
1.04HisIle: 1.04 ± 0.253
0.644HisLys: 0.644 ± 0.224
1.683HisLeu: 1.683 ± 0.284
0.347HisMet: 0.347 ± 0.123
0.792HisAsn: 0.792 ± 0.23
0.891HisPro: 0.891 ± 0.238
0.842HisGln: 0.842 ± 0.171
1.436HisArg: 1.436 ± 0.374
0.743HisSer: 0.743 ± 0.236
0.693HisThr: 0.693 ± 0.18
1.04HisVal: 1.04 ± 0.227
0.297HisTrp: 0.297 ± 0.104
0.693HisTyr: 0.693 ± 0.228
0.0HisXaa: 0.0 ± 0.0
Ile
5.149IleAla: 5.149 ± 0.46
0.594IleCys: 0.594 ± 0.206
3.862IleAsp: 3.862 ± 0.503
4.258IleGlu: 4.258 ± 0.483
1.535IlePhe: 1.535 ± 0.279
4.01IleGly: 4.01 ± 0.498
1.337IleHis: 1.337 ± 0.3
2.971IleIle: 2.971 ± 0.47
2.426IleLys: 2.426 ± 0.301
3.119IleLeu: 3.119 ± 0.425
1.139IleMet: 1.139 ± 0.206
2.08IleAsn: 2.08 ± 0.321
2.377IlePro: 2.377 ± 0.341
2.03IleGln: 2.03 ± 0.488
2.624IleArg: 2.624 ± 0.347
2.723IleSer: 2.723 ± 0.446
3.515IleThr: 3.515 ± 0.393
2.525IleVal: 2.525 ± 0.321
0.495IleTrp: 0.495 ± 0.156
1.485IleTyr: 1.485 ± 0.248
0.0IleXaa: 0.0 ± 0.0
Lys
6.981LysAla: 6.981 ± 0.771
0.495LysCys: 0.495 ± 0.193
2.872LysAsp: 2.872 ± 0.385
3.119LysGlu: 3.119 ± 0.414
1.634LysPhe: 1.634 ± 0.292
3.862LysGly: 3.862 ± 0.388
0.891LysHis: 0.891 ± 0.165
2.723LysIle: 2.723 ± 0.351
2.476LysLys: 2.476 ± 0.318
4.159LysLeu: 4.159 ± 0.422
1.535LysMet: 1.535 ± 0.284
2.278LysAsn: 2.278 ± 0.399
2.525LysPro: 2.525 ± 0.396
2.476LysGln: 2.476 ± 0.361
3.763LysArg: 3.763 ± 0.422
2.921LysSer: 2.921 ± 0.416
3.565LysThr: 3.565 ± 0.413
2.872LysVal: 2.872 ± 0.382
1.04LysTrp: 1.04 ± 0.181
1.386LysTyr: 1.386 ± 0.244
0.0LysXaa: 0.0 ± 0.0
Leu
8.269LeuAla: 8.269 ± 0.553
0.743LeuCys: 0.743 ± 0.209
4.357LeuAsp: 4.357 ± 0.486
3.961LeuGlu: 3.961 ± 0.412
1.881LeuPhe: 1.881 ± 0.354
4.605LeuGly: 4.605 ± 0.562
1.535LeuHis: 1.535 ± 0.284
2.872LeuIle: 2.872 ± 0.369
4.506LeuLys: 4.506 ± 0.51
4.704LeuLeu: 4.704 ± 0.453
1.98LeuMet: 1.98 ± 0.404
2.773LeuAsn: 2.773 ± 0.348
4.11LeuPro: 4.11 ± 0.439
2.773LeuGln: 2.773 ± 0.431
5.545LeuArg: 5.545 ± 0.712
4.704LeuSer: 4.704 ± 0.365
6.189LeuThr: 6.189 ± 0.609
5.743LeuVal: 5.743 ± 0.574
1.139LeuTrp: 1.139 ± 0.223
1.98LeuTyr: 1.98 ± 0.322
0.0LeuXaa: 0.0 ± 0.0
Met
2.723MetAla: 2.723 ± 0.35
0.198MetCys: 0.198 ± 0.11
1.287MetAsp: 1.287 ± 0.252
1.436MetGlu: 1.436 ± 0.294
1.139MetPhe: 1.139 ± 0.223
2.228MetGly: 2.228 ± 0.324
0.644MetHis: 0.644 ± 0.171
1.089MetIle: 1.089 ± 0.266
1.782MetLys: 1.782 ± 0.283
2.525MetLeu: 2.525 ± 0.485
1.188MetMet: 1.188 ± 0.285
1.287MetAsn: 1.287 ± 0.192
1.832MetPro: 1.832 ± 0.341
1.337MetGln: 1.337 ± 0.295
1.485MetArg: 1.485 ± 0.356
1.98MetSer: 1.98 ± 0.349
1.535MetThr: 1.535 ± 0.231
1.188MetVal: 1.188 ± 0.24
0.297MetTrp: 0.297 ± 0.193
0.495MetTyr: 0.495 ± 0.156
0.0MetXaa: 0.0 ± 0.0
Asn
4.902AsnAla: 4.902 ± 0.577
0.297AsnCys: 0.297 ± 0.134
2.426AsnAsp: 2.426 ± 0.342
1.881AsnGlu: 1.881 ± 0.304
1.238AsnPhe: 1.238 ± 0.196
4.357AsnGly: 4.357 ± 0.646
0.495AsnHis: 0.495 ± 0.139
1.436AsnIle: 1.436 ± 0.242
1.832AsnLys: 1.832 ± 0.295
2.377AsnLeu: 2.377 ± 0.289
0.941AsnMet: 0.941 ± 0.161
1.634AsnAsn: 1.634 ± 0.338
1.832AsnPro: 1.832 ± 0.307
1.881AsnGln: 1.881 ± 0.341
2.426AsnArg: 2.426 ± 0.383
2.822AsnSer: 2.822 ± 0.374
2.179AsnThr: 2.179 ± 0.425
2.278AsnVal: 2.278 ± 0.306
0.545AsnTrp: 0.545 ± 0.216
1.139AsnTyr: 1.139 ± 0.244
0.0AsnXaa: 0.0 ± 0.0
Pro
5.644ProAla: 5.644 ± 0.76
0.347ProCys: 0.347 ± 0.138
3.367ProAsp: 3.367 ± 0.284
3.812ProGlu: 3.812 ± 0.493
1.683ProPhe: 1.683 ± 0.318
3.169ProGly: 3.169 ± 0.432
0.99ProHis: 0.99 ± 0.258
1.634ProIle: 1.634 ± 0.269
2.179ProLys: 2.179 ± 0.378
3.02ProLeu: 3.02 ± 0.321
1.139ProMet: 1.139 ± 0.253
1.782ProAsn: 1.782 ± 0.322
1.881ProPro: 1.881 ± 0.306
1.931ProGln: 1.931 ± 0.323
2.228ProArg: 2.228 ± 0.289
2.228ProSer: 2.228 ± 0.539
2.822ProThr: 2.822 ± 0.427
4.456ProVal: 4.456 ± 0.564
0.446ProTrp: 0.446 ± 0.145
1.584ProTyr: 1.584 ± 0.259
0.0ProXaa: 0.0 ± 0.0
Gln
6.239GlnAla: 6.239 ± 0.668
0.545GlnCys: 0.545 ± 0.181
2.228GlnAsp: 2.228 ± 0.388
2.723GlnGlu: 2.723 ± 0.465
1.386GlnPhe: 1.386 ± 0.328
2.921GlnGly: 2.921 ± 0.392
0.842GlnHis: 0.842 ± 0.201
2.377GlnIle: 2.377 ± 0.383
2.377GlnLys: 2.377 ± 0.346
3.565GlnLeu: 3.565 ± 0.516
1.485GlnMet: 1.485 ± 0.256
1.634GlnAsn: 1.634 ± 0.395
2.377GlnPro: 2.377 ± 0.342
5.496GlnGln: 5.496 ± 1.048
3.664GlnArg: 3.664 ± 0.498
2.921GlnSer: 2.921 ± 0.357
1.881GlnThr: 1.881 ± 0.387
2.426GlnVal: 2.426 ± 0.318
1.139GlnTrp: 1.139 ± 0.217
1.535GlnTyr: 1.535 ± 0.233
0.0GlnXaa: 0.0 ± 0.0
Arg
6.041ArgAla: 6.041 ± 0.612
0.594ArgCys: 0.594 ± 0.242
4.357ArgAsp: 4.357 ± 0.534
4.555ArgGlu: 4.555 ± 0.548
2.476ArgPhe: 2.476 ± 0.341
3.614ArgGly: 3.614 ± 0.354
1.535ArgHis: 1.535 ± 0.4
3.614ArgIle: 3.614 ± 0.321
3.367ArgLys: 3.367 ± 0.511
5.595ArgLeu: 5.595 ± 0.544
1.634ArgMet: 1.634 ± 0.338
2.03ArgAsn: 2.03 ± 0.311
2.575ArgPro: 2.575 ± 0.348
3.317ArgGln: 3.317 ± 0.423
4.407ArgArg: 4.407 ± 0.557
3.515ArgSer: 3.515 ± 0.536
2.674ArgThr: 2.674 ± 0.322
3.119ArgVal: 3.119 ± 0.413
0.941ArgTrp: 0.941 ± 0.234
2.03ArgTyr: 2.03 ± 0.347
0.0ArgXaa: 0.0 ± 0.0
Ser
5.892SerAla: 5.892 ± 0.616
0.446SerCys: 0.446 ± 0.177
3.812SerAsp: 3.812 ± 0.403
3.02SerGlu: 3.02 ± 0.338
1.584SerPhe: 1.584 ± 0.315
6.041SerGly: 6.041 ± 0.583
0.891SerHis: 0.891 ± 0.216
3.812SerIle: 3.812 ± 0.459
2.773SerLys: 2.773 ± 0.477
3.466SerLeu: 3.466 ± 0.495
1.584SerMet: 1.584 ± 0.326
2.129SerAsn: 2.129 ± 0.335
3.169SerPro: 3.169 ± 0.325
2.575SerGln: 2.575 ± 0.415
3.02SerArg: 3.02 ± 0.524
3.169SerSer: 3.169 ± 0.457
3.169SerThr: 3.169 ± 0.38
3.466SerVal: 3.466 ± 0.377
1.386SerTrp: 1.386 ± 0.248
1.386SerTyr: 1.386 ± 0.293
0.0SerXaa: 0.0 ± 0.0
Thr
7.179ThrAla: 7.179 ± 0.708
0.396ThrCys: 0.396 ± 0.143
3.515ThrAsp: 3.515 ± 0.545
2.921ThrGlu: 2.921 ± 0.399
1.782ThrPhe: 1.782 ± 0.281
5.496ThrGly: 5.496 ± 0.557
0.545ThrHis: 0.545 ± 0.168
2.525ThrIle: 2.525 ± 0.472
2.129ThrLys: 2.129 ± 0.351
4.852ThrLeu: 4.852 ± 0.607
1.337ThrMet: 1.337 ± 0.237
2.129ThrAsn: 2.129 ± 0.324
3.763ThrPro: 3.763 ± 0.431
2.426ThrGln: 2.426 ± 0.41
2.971ThrArg: 2.971 ± 0.349
3.268ThrSer: 3.268 ± 0.525
3.268ThrThr: 3.268 ± 0.511
4.159ThrVal: 4.159 ± 0.471
1.089ThrTrp: 1.089 ± 0.243
1.238ThrTyr: 1.238 ± 0.224
0.0ThrXaa: 0.0 ± 0.0
Val
6.239ValAla: 6.239 ± 0.556
0.594ValCys: 0.594 ± 0.222
4.159ValAsp: 4.159 ± 0.481
3.911ValGlu: 3.911 ± 0.438
1.485ValPhe: 1.485 ± 0.267
4.555ValGly: 4.555 ± 0.59
0.693ValHis: 0.693 ± 0.185
3.515ValIle: 3.515 ± 0.376
3.268ValLys: 3.268 ± 0.465
3.911ValLeu: 3.911 ± 0.598
1.832ValMet: 1.832 ± 0.271
2.525ValAsn: 2.525 ± 0.384
3.812ValPro: 3.812 ± 0.433
2.624ValGln: 2.624 ± 0.261
3.812ValArg: 3.812 ± 0.481
3.565ValSer: 3.565 ± 0.481
4.407ValThr: 4.407 ± 0.541
4.308ValVal: 4.308 ± 0.484
0.891ValTrp: 0.891 ± 0.247
1.782ValTyr: 1.782 ± 0.303
0.0ValXaa: 0.0 ± 0.0
Trp
1.881TrpAla: 1.881 ± 0.332
0.248TrpCys: 0.248 ± 0.126
1.188TrpAsp: 1.188 ± 0.183
0.693TrpGlu: 0.693 ± 0.174
0.891TrpPhe: 0.891 ± 0.152
0.792TrpGly: 0.792 ± 0.208
0.396TrpHis: 0.396 ± 0.128
0.891TrpIle: 0.891 ± 0.242
0.891TrpLys: 0.891 ± 0.221
1.683TrpLeu: 1.683 ± 0.293
0.347TrpMet: 0.347 ± 0.123
0.545TrpAsn: 0.545 ± 0.208
0.644TrpPro: 0.644 ± 0.168
0.693TrpGln: 0.693 ± 0.223
1.139TrpArg: 1.139 ± 0.291
1.04TrpSer: 1.04 ± 0.179
0.743TrpThr: 0.743 ± 0.159
0.792TrpVal: 0.792 ± 0.212
0.495TrpTrp: 0.495 ± 0.214
0.545TrpTyr: 0.545 ± 0.135
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.723TyrAla: 2.723 ± 0.341
0.545TyrCys: 0.545 ± 0.212
1.931TyrAsp: 1.931 ± 0.275
1.782TyrGlu: 1.782 ± 0.316
1.337TyrPhe: 1.337 ± 0.244
1.881TyrGly: 1.881 ± 0.255
0.743TyrHis: 0.743 ± 0.27
0.99TyrIle: 0.99 ± 0.296
1.139TyrLys: 1.139 ± 0.215
2.426TyrLeu: 2.426 ± 0.366
0.545TyrMet: 0.545 ± 0.174
1.238TyrAsn: 1.238 ± 0.279
1.238TyrPro: 1.238 ± 0.28
1.634TyrGln: 1.634 ± 0.331
2.822TyrArg: 2.822 ± 0.393
2.179TyrSer: 2.179 ± 0.274
1.089TyrThr: 1.089 ± 0.354
1.584TyrVal: 1.584 ± 0.327
0.495TyrTrp: 0.495 ± 0.204
0.743TyrTyr: 0.743 ± 0.195
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 87 proteins (20198 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski