Streptococcus phage SpSL1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A0YRU6|A0A0A0YRU6_9CAUD Uncharacterized protein OS=Streptococcus phage SpSL1 OX=1566990 GN=SpSL1_12 PE=4 SV=1
MM1 pKa = 7.17KK2 pKa = 10.44QFIKK6 pKa = 10.82DD7 pKa = 3.72CFDD10 pKa = 3.72EE11 pKa = 5.42SDD13 pKa = 3.89EE14 pKa = 4.47NDD16 pKa = 3.69SITITFSNGDD26 pKa = 3.92KK27 pKa = 10.33IDD29 pKa = 3.77FFQVYY34 pKa = 9.92DD35 pKa = 4.11DD36 pKa = 5.39CSDD39 pKa = 3.65TANHH43 pKa = 6.04IVLVEE48 pKa = 3.88VEE50 pKa = 3.88TDD52 pKa = 3.63FRR54 pKa = 11.84HH55 pKa = 6.13LVNLDD60 pKa = 3.51YY61 pKa = 11.02VVHH64 pKa = 6.27IRR66 pKa = 11.84SNAA69 pKa = 3.12

Molecular weight: 8.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A0YQR3|A0A0A0YQR3_9CAUD HTH cro/C1-type domain-containing protein OS=Streptococcus phage SpSL1 OX=1566990 GN=SpSL1_05 PE=4 SV=1
MM1 pKa = 7.32SVDD4 pKa = 3.06IKK6 pKa = 10.89AIRR9 pKa = 11.84WLLDD13 pKa = 2.92NATAYY18 pKa = 10.25AISKK22 pKa = 9.18NCGVSIQAIDD32 pKa = 3.38KK33 pKa = 9.24YY34 pKa = 11.2KK35 pKa = 11.14NGVSDD40 pKa = 3.87IMNMRR45 pKa = 11.84LKK47 pKa = 10.58HH48 pKa = 6.36AISMTAYY55 pKa = 10.22AHH57 pKa = 5.78TLRR60 pKa = 11.84EE61 pKa = 4.2KK62 pKa = 10.55QQ63 pKa = 3.2

Molecular weight: 7.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

10492

42

1602

209.8

23.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.357 ± 0.626

0.753 ± 0.138

6.109 ± 0.275

7.73 ± 0.318

4.165 ± 0.19

5.804 ± 0.333

1.63 ± 0.188

7.101 ± 0.267

8.378 ± 0.375

8.578 ± 0.421

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.373 ± 0.191

5.957 ± 0.243

2.535 ± 0.172

4.117 ± 0.227

4.518 ± 0.291

6.5 ± 0.377

6.014 ± 0.306

5.928 ± 0.273

1.506 ± 0.137

3.946 ± 0.396
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski