Amino acid dipepetide frequency for Streptococcus phage SpSL1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.288AlaAla: 2.288 ± 0.848
0.572AlaCys: 0.572 ± 0.287
4.575AlaAsp: 4.575 ± 0.532
5.529AlaGlu: 5.529 ± 0.879
2.288AlaPhe: 2.288 ± 0.437
3.622AlaGly: 3.622 ± 0.639
0.953AlaHis: 0.953 ± 0.289
5.719AlaIle: 5.719 ± 1.459
4.957AlaLys: 4.957 ± 0.754
5.719AlaLeu: 5.719 ± 0.706
1.906AlaMet: 1.906 ± 0.567
3.622AlaAsn: 3.622 ± 0.99
1.43AlaPro: 1.43 ± 0.417
2.669AlaGln: 2.669 ± 0.454
2.574AlaArg: 2.574 ± 0.485
3.527AlaSer: 3.527 ± 0.482
3.622AlaThr: 3.622 ± 0.66
3.908AlaVal: 3.908 ± 0.617
1.334AlaTrp: 1.334 ± 0.38
2.764AlaTyr: 2.764 ± 0.452
0.0AlaXaa: 0.0 ± 0.0
Cys
0.286CysAla: 0.286 ± 0.159
0.0CysCys: 0.0 ± 0.0
0.286CysAsp: 0.286 ± 0.166
0.858CysGlu: 0.858 ± 0.342
0.286CysPhe: 0.286 ± 0.161
0.477CysGly: 0.477 ± 0.243
0.572CysHis: 0.572 ± 0.22
0.191CysIle: 0.191 ± 0.142
0.381CysLys: 0.381 ± 0.204
0.953CysLeu: 0.953 ± 0.286
0.095CysMet: 0.095 ± 0.09
0.572CysAsn: 0.572 ± 0.347
0.095CysPro: 0.095 ± 0.101
0.191CysGln: 0.191 ± 0.123
0.477CysArg: 0.477 ± 0.226
0.381CysSer: 0.381 ± 0.203
0.381CysThr: 0.381 ± 0.218
0.381CysVal: 0.381 ± 0.205
0.191CysTrp: 0.191 ± 0.103
0.477CysTyr: 0.477 ± 0.198
0.0CysXaa: 0.0 ± 0.0
Asp
3.146AspAla: 3.146 ± 0.688
0.667AspCys: 0.667 ± 0.344
3.527AspAsp: 3.527 ± 0.62
4.099AspGlu: 4.099 ± 0.805
3.05AspPhe: 3.05 ± 0.555
4.385AspGly: 4.385 ± 0.733
0.858AspHis: 0.858 ± 0.275
4.671AspIle: 4.671 ± 0.66
5.243AspLys: 5.243 ± 0.677
4.575AspLeu: 4.575 ± 0.542
1.62AspMet: 1.62 ± 0.459
3.336AspAsn: 3.336 ± 0.56
2.097AspPro: 2.097 ± 0.496
1.43AspGln: 1.43 ± 0.483
2.383AspArg: 2.383 ± 0.477
3.622AspSer: 3.622 ± 0.464
3.717AspThr: 3.717 ± 0.49
4.099AspVal: 4.099 ± 0.432
0.953AspTrp: 0.953 ± 0.241
3.622AspTyr: 3.622 ± 0.611
0.0AspXaa: 0.0 ± 0.0
Glu
4.957GluAla: 4.957 ± 0.922
0.572GluCys: 0.572 ± 0.34
4.003GluAsp: 4.003 ± 0.629
6.863GluGlu: 6.863 ± 0.963
2.288GluPhe: 2.288 ± 0.439
2.478GluGly: 2.478 ± 0.513
0.953GluHis: 0.953 ± 0.327
6.577GluIle: 6.577 ± 0.768
7.626GluLys: 7.626 ± 1.569
9.151GluLeu: 9.151 ± 0.919
1.811GluMet: 1.811 ± 0.446
4.194GluAsn: 4.194 ± 0.769
2.002GluPro: 2.002 ± 0.374
3.241GluGln: 3.241 ± 0.616
3.432GluArg: 3.432 ± 0.53
4.194GluSer: 4.194 ± 0.545
4.671GluThr: 4.671 ± 0.499
4.861GluVal: 4.861 ± 0.555
1.525GluTrp: 1.525 ± 0.456
1.906GluTyr: 1.906 ± 0.397
0.0GluXaa: 0.0 ± 0.0
Phe
1.906PheAla: 1.906 ± 0.442
0.191PheCys: 0.191 ± 0.165
4.099PheAsp: 4.099 ± 0.576
3.813PheGlu: 3.813 ± 0.508
1.62PhePhe: 1.62 ± 0.333
2.002PheGly: 2.002 ± 0.472
0.667PheHis: 0.667 ± 0.239
3.146PheIle: 3.146 ± 0.656
3.527PheLys: 3.527 ± 0.718
2.86PheLeu: 2.86 ± 0.796
1.43PheMet: 1.43 ± 0.439
2.955PheAsn: 2.955 ± 0.468
0.858PhePro: 0.858 ± 0.318
1.049PheGln: 1.049 ± 0.295
2.002PheArg: 2.002 ± 0.445
3.05PheSer: 3.05 ± 0.62
2.669PheThr: 2.669 ± 0.471
1.906PheVal: 1.906 ± 0.562
0.763PheTrp: 0.763 ± 0.255
1.144PheTyr: 1.144 ± 0.327
0.0PheXaa: 0.0 ± 0.0
Gly
3.622GlyAla: 3.622 ± 1.079
0.286GlyCys: 0.286 ± 0.169
3.05GlyAsp: 3.05 ± 0.487
3.432GlyGlu: 3.432 ± 0.659
2.955GlyPhe: 2.955 ± 0.514
3.813GlyGly: 3.813 ± 0.567
1.049GlyHis: 1.049 ± 0.389
4.48GlyIle: 4.48 ± 0.581
4.957GlyLys: 4.957 ± 0.581
5.338GlyLeu: 5.338 ± 0.876
1.239GlyMet: 1.239 ± 0.449
3.813GlyAsn: 3.813 ± 0.579
0.667GlyPro: 0.667 ± 0.299
2.574GlyGln: 2.574 ± 0.602
2.764GlyArg: 2.764 ± 0.469
3.908GlySer: 3.908 ± 0.61
2.955GlyThr: 2.955 ± 0.502
3.527GlyVal: 3.527 ± 0.565
1.334GlyTrp: 1.334 ± 0.483
2.288GlyTyr: 2.288 ± 0.367
0.0GlyXaa: 0.0 ± 0.0
His
0.667HisAla: 0.667 ± 0.234
0.191HisCys: 0.191 ± 0.163
1.049HisAsp: 1.049 ± 0.347
1.43HisGlu: 1.43 ± 0.454
0.953HisPhe: 0.953 ± 0.256
0.667HisGly: 0.667 ± 0.254
0.0HisHis: 0.0 ± 0.0
2.192HisIle: 2.192 ± 0.435
0.572HisLys: 0.572 ± 0.21
1.811HisLeu: 1.811 ± 0.545
0.381HisMet: 0.381 ± 0.162
0.667HisAsn: 0.667 ± 0.254
0.381HisPro: 0.381 ± 0.204
0.572HisGln: 0.572 ± 0.259
0.381HisArg: 0.381 ± 0.218
0.763HisSer: 0.763 ± 0.327
1.62HisThr: 1.62 ± 0.469
1.239HisVal: 1.239 ± 0.355
0.0HisTrp: 0.0 ± 0.0
0.763HisTyr: 0.763 ± 0.24
0.0HisXaa: 0.0 ± 0.0
Ile
5.624IleAla: 5.624 ± 0.764
0.763IleCys: 0.763 ± 0.396
4.957IleAsp: 4.957 ± 0.767
5.529IleGlu: 5.529 ± 0.756
2.574IlePhe: 2.574 ± 0.463
4.003IleGly: 4.003 ± 0.693
1.239IleHis: 1.239 ± 0.308
3.622IleIle: 3.622 ± 0.765
6.482IleLys: 6.482 ± 0.827
5.243IleLeu: 5.243 ± 0.773
1.62IleMet: 1.62 ± 0.375
4.957IleAsn: 4.957 ± 0.653
2.097IlePro: 2.097 ± 0.381
3.146IleGln: 3.146 ± 0.438
2.955IleArg: 2.955 ± 0.646
5.147IleSer: 5.147 ± 0.705
4.575IleThr: 4.575 ± 0.636
3.241IleVal: 3.241 ± 0.61
0.858IleTrp: 0.858 ± 0.343
2.383IleTyr: 2.383 ± 0.743
0.0IleXaa: 0.0 ± 0.0
Lys
6.482LysAla: 6.482 ± 0.875
0.191LysCys: 0.191 ± 0.147
5.147LysAsp: 5.147 ± 0.783
7.244LysGlu: 7.244 ± 0.815
2.86LysPhe: 2.86 ± 0.501
4.671LysGly: 4.671 ± 0.806
1.525LysHis: 1.525 ± 0.392
5.624LysIle: 5.624 ± 0.642
6.291LysLys: 6.291 ± 0.974
7.34LysLeu: 7.34 ± 0.999
2.192LysMet: 2.192 ± 0.456
4.099LysAsn: 4.099 ± 0.616
1.62LysPro: 1.62 ± 0.365
4.289LysGln: 4.289 ± 0.644
3.813LysArg: 3.813 ± 0.683
5.719LysSer: 5.719 ± 0.634
5.433LysThr: 5.433 ± 0.762
4.003LysVal: 4.003 ± 0.676
1.239LysTrp: 1.239 ± 0.29
4.003LysTyr: 4.003 ± 0.815
0.0LysXaa: 0.0 ± 0.0
Leu
6.482LeuAla: 6.482 ± 0.782
0.953LeuCys: 0.953 ± 0.371
5.433LeuAsp: 5.433 ± 0.555
6.577LeuGlu: 6.577 ± 0.977
3.622LeuPhe: 3.622 ± 0.621
5.052LeuGly: 5.052 ± 0.811
1.716LeuHis: 1.716 ± 0.438
5.719LeuIle: 5.719 ± 0.699
7.435LeuLys: 7.435 ± 0.873
5.338LeuLeu: 5.338 ± 0.934
2.86LeuMet: 2.86 ± 0.731
4.861LeuAsn: 4.861 ± 0.78
2.288LeuPro: 2.288 ± 0.517
2.764LeuGln: 2.764 ± 0.565
3.717LeuArg: 3.717 ± 0.666
6.863LeuSer: 6.863 ± 0.983
5.433LeuThr: 5.433 ± 0.838
4.766LeuVal: 4.766 ± 0.871
0.667LeuTrp: 0.667 ± 0.395
3.241LeuTyr: 3.241 ± 0.595
0.0LeuXaa: 0.0 ± 0.0
Met
1.716MetAla: 1.716 ± 0.37
0.286MetCys: 0.286 ± 0.185
1.334MetAsp: 1.334 ± 0.33
1.525MetGlu: 1.525 ± 0.53
0.572MetPhe: 0.572 ± 0.261
1.334MetGly: 1.334 ± 0.292
0.0MetHis: 0.0 ± 0.0
1.239MetIle: 1.239 ± 0.474
3.146MetLys: 3.146 ± 0.61
1.716MetLeu: 1.716 ± 0.424
0.667MetMet: 0.667 ± 0.264
2.097MetAsn: 2.097 ± 0.494
0.763MetPro: 0.763 ± 0.281
0.667MetGln: 0.667 ± 0.233
1.43MetArg: 1.43 ± 0.413
1.811MetSer: 1.811 ± 0.593
1.049MetThr: 1.049 ± 0.363
1.811MetVal: 1.811 ± 0.395
0.191MetTrp: 0.191 ± 0.122
0.286MetTyr: 0.286 ± 0.151
0.0MetXaa: 0.0 ± 0.0
Asn
2.764AsnAla: 2.764 ± 0.633
0.286AsnCys: 0.286 ± 0.15
3.336AsnAsp: 3.336 ± 0.69
4.861AsnGlu: 4.861 ± 0.516
2.002AsnPhe: 2.002 ± 0.401
4.194AsnGly: 4.194 ± 0.695
1.144AsnHis: 1.144 ± 0.389
3.432AsnIle: 3.432 ± 0.456
4.766AsnLys: 4.766 ± 0.601
4.957AsnLeu: 4.957 ± 0.746
1.239AsnMet: 1.239 ± 0.51
3.622AsnAsn: 3.622 ± 0.578
2.288AsnPro: 2.288 ± 0.348
3.336AsnGln: 3.336 ± 0.572
3.146AsnArg: 3.146 ± 0.427
4.099AsnSer: 4.099 ± 0.662
3.05AsnThr: 3.05 ± 0.374
3.908AsnVal: 3.908 ± 0.516
0.763AsnTrp: 0.763 ± 0.265
2.383AsnTyr: 2.383 ± 0.541
0.0AsnXaa: 0.0 ± 0.0
Pro
1.906ProAla: 1.906 ± 0.353
0.286ProCys: 0.286 ± 0.171
1.906ProAsp: 1.906 ± 0.433
1.43ProGlu: 1.43 ± 0.361
0.763ProPhe: 0.763 ± 0.291
0.858ProGly: 0.858 ± 0.264
0.572ProHis: 0.572 ± 0.247
1.62ProIle: 1.62 ± 0.397
2.383ProLys: 2.383 ± 0.408
2.383ProLeu: 2.383 ± 0.378
0.286ProMet: 0.286 ± 0.172
0.953ProAsn: 0.953 ± 0.312
0.191ProPro: 0.191 ± 0.134
1.62ProGln: 1.62 ± 0.365
1.049ProArg: 1.049 ± 0.267
1.525ProSer: 1.525 ± 0.494
2.097ProThr: 2.097 ± 0.429
2.288ProVal: 2.288 ± 0.366
0.477ProTrp: 0.477 ± 0.168
0.763ProTyr: 0.763 ± 0.281
0.0ProXaa: 0.0 ± 0.0
Gln
3.717GlnAla: 3.717 ± 0.656
0.191GlnCys: 0.191 ± 0.103
1.239GlnAsp: 1.239 ± 0.3
3.813GlnGlu: 3.813 ± 0.758
1.62GlnPhe: 1.62 ± 0.381
2.955GlnGly: 2.955 ± 0.538
0.763GlnHis: 0.763 ± 0.27
3.05GlnIle: 3.05 ± 0.508
3.05GlnLys: 3.05 ± 0.547
3.813GlnLeu: 3.813 ± 0.581
0.858GlnMet: 0.858 ± 0.284
2.288GlnAsn: 2.288 ± 0.563
1.049GlnPro: 1.049 ± 0.358
1.62GlnGln: 1.62 ± 0.447
2.002GlnArg: 2.002 ± 0.493
2.764GlnSer: 2.764 ± 0.836
2.002GlnThr: 2.002 ± 0.575
2.955GlnVal: 2.955 ± 0.717
0.286GlnTrp: 0.286 ± 0.161
1.144GlnTyr: 1.144 ± 0.473
0.0GlnXaa: 0.0 ± 0.0
Arg
2.669ArgAla: 2.669 ± 0.4
0.286ArgCys: 0.286 ± 0.176
1.716ArgAsp: 1.716 ± 0.379
3.527ArgGlu: 3.527 ± 0.615
1.811ArgPhe: 1.811 ± 0.364
1.906ArgGly: 1.906 ± 0.516
0.667ArgHis: 0.667 ± 0.255
3.813ArgIle: 3.813 ± 0.632
4.385ArgLys: 4.385 ± 0.446
4.671ArgLeu: 4.671 ± 0.571
0.858ArgMet: 0.858 ± 0.307
2.955ArgAsn: 2.955 ± 0.745
1.525ArgPro: 1.525 ± 0.422
2.097ArgGln: 2.097 ± 0.494
2.383ArgArg: 2.383 ± 0.616
2.097ArgSer: 2.097 ± 0.346
1.811ArgThr: 1.811 ± 0.505
2.383ArgVal: 2.383 ± 0.531
0.953ArgTrp: 0.953 ± 0.227
2.669ArgTyr: 2.669 ± 0.602
0.0ArgXaa: 0.0 ± 0.0
Ser
4.671SerAla: 4.671 ± 1.07
0.381SerCys: 0.381 ± 0.208
4.194SerAsp: 4.194 ± 0.734
4.957SerGlu: 4.957 ± 0.845
3.05SerPhe: 3.05 ± 0.409
4.289SerGly: 4.289 ± 0.649
0.667SerHis: 0.667 ± 0.304
4.671SerIle: 4.671 ± 0.703
4.385SerLys: 4.385 ± 0.55
5.052SerLeu: 5.052 ± 0.892
0.953SerMet: 0.953 ± 0.261
4.289SerAsn: 4.289 ± 0.799
1.62SerPro: 1.62 ± 0.445
2.669SerGln: 2.669 ± 0.564
2.478SerArg: 2.478 ± 0.495
5.433SerSer: 5.433 ± 1.174
4.289SerThr: 4.289 ± 0.869
4.099SerVal: 4.099 ± 0.879
0.953SerTrp: 0.953 ± 0.365
1.906SerTyr: 1.906 ± 0.373
0.0SerXaa: 0.0 ± 0.0
Thr
4.671ThrAla: 4.671 ± 0.83
0.477ThrCys: 0.477 ± 0.228
3.908ThrAsp: 3.908 ± 0.697
2.669ThrGlu: 2.669 ± 0.467
2.86ThrPhe: 2.86 ± 0.732
3.908ThrGly: 3.908 ± 0.862
0.667ThrHis: 0.667 ± 0.234
4.957ThrIle: 4.957 ± 0.707
4.575ThrLys: 4.575 ± 0.473
4.861ThrLeu: 4.861 ± 0.611
0.858ThrMet: 0.858 ± 0.295
3.336ThrAsn: 3.336 ± 0.513
0.953ThrPro: 0.953 ± 0.466
2.669ThrGln: 2.669 ± 0.513
3.527ThrArg: 3.527 ± 0.678
3.432ThrSer: 3.432 ± 0.547
5.052ThrThr: 5.052 ± 0.963
3.527ThrVal: 3.527 ± 0.599
0.858ThrTrp: 0.858 ± 0.289
2.383ThrTyr: 2.383 ± 0.467
0.0ThrXaa: 0.0 ± 0.0
Val
2.955ValAla: 2.955 ± 0.66
0.381ValCys: 0.381 ± 0.185
4.289ValAsp: 4.289 ± 0.571
5.338ValGlu: 5.338 ± 0.609
2.955ValPhe: 2.955 ± 0.755
4.194ValGly: 4.194 ± 0.915
0.858ValHis: 0.858 ± 0.274
2.955ValIle: 2.955 ± 0.459
5.243ValLys: 5.243 ± 0.887
4.099ValLeu: 4.099 ± 0.788
1.43ValMet: 1.43 ± 0.286
4.003ValAsn: 4.003 ± 0.526
1.906ValPro: 1.906 ± 0.326
1.811ValGln: 1.811 ± 0.423
2.192ValArg: 2.192 ± 0.49
3.622ValSer: 3.622 ± 0.608
4.003ValThr: 4.003 ± 0.559
4.194ValVal: 4.194 ± 0.721
0.858ValTrp: 0.858 ± 0.257
2.002ValTyr: 2.002 ± 0.507
0.0ValXaa: 0.0 ± 0.0
Trp
0.667TrpAla: 0.667 ± 0.229
0.191TrpCys: 0.191 ± 0.141
0.381TrpAsp: 0.381 ± 0.147
0.572TrpGlu: 0.572 ± 0.191
0.953TrpPhe: 0.953 ± 0.277
0.953TrpGly: 0.953 ± 0.239
0.286TrpHis: 0.286 ± 0.18
1.334TrpIle: 1.334 ± 0.342
1.144TrpLys: 1.144 ± 0.411
1.62TrpLeu: 1.62 ± 0.506
0.477TrpMet: 0.477 ± 0.208
1.43TrpAsn: 1.43 ± 0.377
0.095TrpPro: 0.095 ± 0.089
0.858TrpGln: 0.858 ± 0.25
0.572TrpArg: 0.572 ± 0.18
1.334TrpSer: 1.334 ± 0.575
0.477TrpThr: 0.477 ± 0.2
0.763TrpVal: 0.763 ± 0.256
0.095TrpTrp: 0.095 ± 0.075
0.858TrpTyr: 0.858 ± 0.551
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.383TyrAla: 2.383 ± 0.543
0.381TyrCys: 0.381 ± 0.162
2.669TyrAsp: 2.669 ± 0.421
3.241TyrGlu: 3.241 ± 0.483
2.574TyrPhe: 2.574 ± 0.572
2.288TyrGly: 2.288 ± 0.441
1.144TyrHis: 1.144 ± 0.343
2.002TyrIle: 2.002 ± 0.508
3.241TyrLys: 3.241 ± 0.622
4.289TyrLeu: 4.289 ± 0.647
0.858TyrMet: 0.858 ± 0.238
1.525TyrAsn: 1.525 ± 0.473
1.43TyrPro: 1.43 ± 0.44
1.906TyrGln: 1.906 ± 0.387
1.906TyrArg: 1.906 ± 0.533
1.716TyrSer: 1.716 ± 0.373
1.239TyrThr: 1.239 ± 0.312
1.43TyrVal: 1.43 ± 0.429
0.763TyrTrp: 0.763 ± 0.231
2.478TyrTyr: 2.478 ± 0.733
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 50 proteins (10492 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski